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Tytuł:
Antibiotic susceptibility of bacteria isolated from respiratory tract of pigs in Poland between 2004 and 2008
Autorzy:
Markowska-Daniel, I
Urbaniak, K.
Stepniewska, K.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/30384.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Polska
pig
respiratory tract
bacteria
isolation
antibiotic susceptibility
animal health
productivity
animal production
Opis:
Antibiotic susceptibility of bacteria isolated from nasal swabs and lungs of pigs, to 16 commonly used antibiotics, was determined by disc diffusion test, ß-lactams showed the best activity against Streptococcus suis (S. suis) (> 99% of susceptible strains). The lowest sensitivity of S. suis was evidenced to: tylosin, tetracycline and neomycin (50%, 40% and 25%, respectively). Isolates of Escherichia coli (E. coli) demonstrated the highest susceptibility to cephalosporin (85% strains), gentamicin and norfloxacin (over 74%). The lowest susceptibility of E. coli was demonstrated to tiamulin and penicillin (11.3% and 1.9%, respectively). Over 80% of Actinobacillus pleuropneumoniae (App) strains were susceptible to all antibiotics tested. The highest resistance of App, but demonstrated by below 20% of tested isolates only, was evidenced to neomycin and LxS. Isolates of Pasteurella multocida (Pm), Haemophilus parasuis (Hps) and Arcanobacterium pyogenes (A. pyogenes) were highly susceptible to the most antibiotics included in the analysis. The comparison of the in vitro susceptibility of pathogens to the chemotherapeutics used on Polish farms for the therapy of bacterial infection of pigs within the last five years and the last 10 years, showed an increasing percent of E. coli and S. suis strains resistant to commonly used antibiotics. It is also shown that Pm, Hps, App and A. pyogenes isolates were continuously susceptible to the most chemotherapeutics applied.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 29-36
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antybiotykooporność bakterii zoonotycznych występujących u zwierząt i w żywności
Autorzy:
Truszczynski, M.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/862197.pdf
Data publikacji:
2010
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
czynniki chorobotworcze
bakterie
bakterie zoonotyczne
opornosc na antybiotyki
Źródło:
Życie Weterynaryjne; 2010, 85, 11
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dynamic changes of immunoglobulin concentrations in pig colostrum and serum around parturition
Autorzy:
Markowska-Daniel, I
Pomorska-Mol, M.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/30942.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pig
serum
colostrum
immunoglobulin
immunity
dynamic change
parturition
perinatal period
piglet
porcine serum
Opis:
The aim of the study was the determination of IgA, IgM and IgG concentrations in porcine serum and colostrum, in order to evaluate their variations in the perinatal period, as well as to clarify whether there is a correlation between colostrum intake, initial level of immunoglobulins (Ig) in piglet serum and development of their own immunity. The mean IgA, IgM and IgG concentrations in sow serum 10 days before parturition were 1.58, 6.12 and 39.56 mg/ml, respectively. Seven days later only the IgG level was insignificantly lower (34.94 mg/ml, p=0.55), while concentrations of IgA and IgM increased to 2.25 and 7.25 mg/ml, respectively (p=0.23 and 0.62, respectively). The mean initial IgG concentration in colostrum at farrowing was 118.5 mg/ml and differed between sows. The average value of IgA in colostrum at birth was 23.8 mg/ml and decreased to 7.85 mg/ml at 6 hours (h) and to 4.59 mg/ml at 24 h after the onset of farrowing. IgM concentration at birth was 12.1 mg/ml and decreased to 4.23 mg/ml at 24 h postpartum. Positive relationships were found between concentrations of IgM and IgA in serum of piglets at 14 and 56 days of life (r = 0.41 and 0.80, respectively, p≤0.05) as well as for IgG concentration in the piglets serum at 7 days and 56 days of age (r = 0.48, p≤0.05). The above observations suggest that there is a correlation between the level of Ig in piglet serum in the first days of life and improvement of their own immunity.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 21-27
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence of leptospiral infections in swine population in Poland evaluated by ELISA and microscopic agglutination test
Autorzy:
Wasinski, B.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/31105.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
occurrence
pig
population
Polska
ELISA test
microscopic agglutination test
leptospirosis
animal infection
Leptospira
serogroup
zoonotic disease
Opis:
Swine are one of significant reservoirs and sources of Leptospira infections for man. Serological screenings help to effectively control the epidemiological situation in swine herds and to prevent transmission of Leptospira from animals to man. The purpose of this study was to investigate, by the use of serological methods, the prevalence of infections caused by selected Leptospira serogroups in swine population in Poland. A total of 7112 swine serum samples were examined. The samples were collected from January to October 2008 and came from 280 counties situated in all 16 provinces of Poland. All sera were examined preliminary by enzyme-linked immunosorbent assay (ELISA) using heat-stable antigenic preparation. The samples positive or doubtful in ELISA were investigated by microscopic agglutination test (MAT) with use of serovars Icterohaemorrhagiae, Pomona, Canicola, Sejroe, Tarassovi and Grippotyphosa. Of the collected sera examined by ELISA 73 (1.02%) samples were positive, 85 (1.20%) – doubtful and 6954 – negative. Among ELISA-positive and doubtful sera 64 samples (coming from 14 provinces) were recognized in MAT as positive. Among MAT positive samples 42.19% of sera demonstrated titres with serovar Pomona, 32.81% – with Sejroe, 14.06% – with Icterohaemorrhagiae, 6.25% – with Tarassovi, 3.13% – with Grippotyphosa and 1.56% with Canicola.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tematyka 21. Kongresu IPVS w Vancouver. Część I. Zakażenia cirkowirusowe świń
Subject-matter of 21st IPVS Congress in Vancouver. Part I. Infections caused by circoviruses in swine
Autorzy:
Pejsak, Z.
Truszczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/857844.pdf
Data publikacji:
2010
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
Miedzynarodowe Towarzystwo Specjalistow Chorob Swin
konferencje
Vancouver konferencja
tematyka
trzoda chlewna
choroby zwierzat
choroby wirusowe
cirkowirusy
wirus PCV-2
infekcja wirusowa
szczepienia ochronne
szczepionki
efektywnosc szczepien
Źródło:
Życie Weterynaryjne; 2010, 85, 12
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wytyczne unijne dotyczące nadzoru, monitoringu, zwalczania oraz eradykacji klasycznego pomoru świń u dzików
European Union guidelines on surveillance, monitoring, control and eradication of classical swine fever in wild boars
Autorzy:
Truszczynski, M.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/858812.pdf
Data publikacji:
2010
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
zwierzeta dziko zyjace
dziki
choroby zwierzat
klasyczny pomor swin
epidemiologia
profilaktyka
szczepienia ochronne
szczepionki doustne
zwalczanie chorob zwierzat
monitoring chorob
nadzor
eradykacja chorob
wymagania unijne
dokument SANCO/7032/2010
Źródło:
Życie Weterynaryjne; 2010, 85, 09
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comparison of immunohistochemistry and in situ hybridization for the detection of porcine circovirus type 2 in pigs
Autorzy:
Szczotka, A.
Stadejek, T.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/30256.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to develop and to optimize an immunohistochemistry (IHC) method for PCV2 identification and to compare it with an in situ hybridization (ISH) technique. The results demonstrated that both ISH and IHC successfully detected PCV2 viral antigens or nucleic acid in the examined tissues. Most of the slides identified previously in ISH as PCV2-positive were also positive in IHC. In the case of nearly half of the slides the results of IHC examination revealed an increase in the intensity of staining. IHC presented higher sensitivity and specificity than ISH. No negative impact of the time of paraffin block storage on ISH detection results was observed. In addition, IHC results were easier to interpret due to better image quality after staining. Overall results confirmed IHC was a reliable and useful technique for PMWS diagnosis.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical detection of Lawsonia intracellularis in tissue section from pigs
Autorzy:
Szczotka, A.
Stadejek, T.
Zmudzki, J.
Nowak, A.
Osinski, Z.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/31904.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the present study was to develop an immunohistochemical method (IHC) for detection of Lawsonia intracellularis (L. intracellularis) in formalin-fixed, paraffin embedded sections of intestines from pigs and to implement this method in differential diagnosis of swine diseases with diarrhea in postweaning pigs. The study was conducted on 165 sections of intestines (ileum, caecum and colon) collected from 76 pigs, representing 42 Polish pig farms. The animals included in the analysis suffered from diarrhea, with bloody or grey to brown feces, and were suspected of porcine proliferative enteropathy (PPE). Sections of intestines were analyzed for the presence of L. intracellularis by polymerase chain reaction (PCR) and IHC. Among 165 intestinal samples from pigs with diarrhea, L. intracellularis DNA was detected by PCR in 33 (20.0%) samples. In this group, 30 samples (18.2% of all the samples tested) were also found positive in IHC, while only 3 (1.8%) were IHC-negative. One hundred thirty-two (80.0%) samples were negative in both tests. The PCR- and IHC-positive samples originated from 11 pigs, 4- to 20-week old, from 8 farms. L. intracellularis antigen was visualized by IHC mostly in intestinal crypts and/or in mononuclear cells of the lamina propria). The positive signal in epithelial cells was observed close to the luminal borders, creating typical specifically stained rims around the crypt lumina.ą The results of the present study further confirm the usefulness of IHC in the detection of L. intracellularis antigen in the intestinal tissues.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Usefulness of PCR/RFLP and ERIC PCR techniques for epidemiological study of Haemophilus parasuis infections in pigs
Autorzy:
Jablonski, A.
Zebek, S.
Kolacz, R.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/30649.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
animal disease
pig
animal infection
epidemiology
Haemophilus parasuis
genotyping
polymerase chain reaction
virulence
diagnosis
microorganism
DNA fragment
electrophoretic separation
environmental factor
molecular method
Opis:
Haemophilus parasuis belongs to opportunistic microorganisms of undefined virulence. The purpose of the studies was to compare suitability of PCR/RFLP in our modification and ERIC PCR for epidemiological study of domestic strains of H. parasuis. The results were evaluated taking into account two different aspects: suitability of the tests for isolating the highest possible number of clone groups and subjective evaluation of the method judged with respect to the following criteria: difficulty, availability of equipment and reagents as well as time and cost of the study. The results obtained in the present study show that the two methods used for typing of H. parasuis had high discriminatory power. Taking into account this parameter it can be concluded that ERIC PCR is more suitable than PCR/RFLP. This justifies the use of ERIC PCR for routine epidemiological analyses of mentioned pathogen. Taking into account the complexity of method used, ERIC-PCR based on random amplification of DNA, proved to be comparable to PCR/RFLP. The last mentioned technique is relatively less expensive and labour-consuming, especially when diagnostic PCR method is used for the epidemiological studies.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antimicrobial susceptibility of Brachyspira hyodysenteriae isolated from 21 Polish farms
Autorzy:
Zmudzki, J.
Szczotka, A.
Nowak, A.
Strzelecka, H.
Grzesiak, A.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/32546.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Swine dysentery (SD) is a common disease among pigs worldwide, which contributes to major production losses. Antimicrobial susceptibility testing of B. hyodysenteriae, the etiological agent of SD, is mainly performed by the agar dilution method. This method has certain limitations due to difficulties in interpretation of results. The aim of this study was the analysis of antimicrobial susceptibility of Brachyspira hyodysenteriae (B. hyodysenteriae) Polish field isolates by broth microdilution procedure. The study was performed on 21 isolates of B. hyodysenteriae, collected between January 2006 to December 2010 from cases of swine dysentery. VetMIC™ Brachyspira panels with antimicrobial agents (tiamulin, valnemulin, doxycycline, lincomycin, tylosin and ampicillin) were used for susceptibility testing of B. hyodysenteriae. The minimal inhibitory concentration (MIC) was determined by the broth dilution procedure. The lowest antimicrobial activity was demonstrated for tylosin and lincomycin, with inhibition of bacterial growth using concentrations >128 μg/ml and 32 μg/ml, respectively. In the case of doxycycline, the MIC values were ≤ 2.0 μg/ml. No decreased susceptibility to tiamulin was found among the Polish isolates and MIC values for this antibiotic did not exceed 1.0 μg/ml. The results of the present study confirmed that Polish B. hyodysenteriae isolates were susceptible to the main antibiotics (tiamulin and valnemulin) used in treatment of swine dysentery. Further studies are necessary to evaluate a possible slow decrease in susceptibility to tiamulin and valnemulin of B. hyodysenteriae strains in Poland.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of real-time PCR for detection of Lawsonia intracellularis and Brachyspira hyodysenteriage in fecal samples from pigs
Autorzy:
Zmudzki, J.
Szczotka, A.
Podgorska, K.
Nowak, A.
Grzesiak, A.
Dors, A.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/30775.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the study was to develop and validate real-time PCR method for the quantification of Lawsonia intracellularis and Brachyspira hyodysenteriae in porcine feces. Before the optimization process was performed two different extraction methods were compared to select the more efficient one. Based on the results achieved at this stage the boiling procedure was rejected and a commercially available silica-membrane based method was chosen for further analysis. The primers and the Taqman probe for B. hyodysenteriae and L. intracellularis were based on the sequence of NADH oxidase gene and 16S rDNA gene, respectively. The detection limit of the real-time PCR for suspension of feces inoculated with B. hyodysenteriae and L. intracellularis was determined to be 1.5x103 CFU/ml and 6.5x101 CFU/ml, respectively. The results of this study demonstrate that our real-time PCR is able to detect low number of B. hyodysenteriae and L. intracellularis cells which is satisfying in routine diagnosis of swine dysentery and proliferative enteropathy. Therefore, it is possible to identify both subclinically infected pigs and those representing an acute form of mentioned diseases. In summary, the quantitative real-time PCR is useful for routine diagnosis of L. intracellularis and B. hyodysenteriae. Compared to conventional PCR, the new validated quantification method based on real-time PCR is fast and with reduced risk of laboratory contamination. The novel technique is specific and even more sensitive than the previously used one. Furthermore, the new real-time PCR enables quick detection and quantification of both pathogens in fecal samples, which helps to estimate the health status of a pig herd.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of PCR methods for detection of classical swine fever virus and other pestiviruses
Autorzy:
Podgorska, K.
Kamieniecka
Stadejek, T.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2088001.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Classical swine fever (CSF) is a notifiable, highly contagious disease of swine controlled mainly with costly administrative methods. Swine may be infected not only with classical swine fever virus (CSFV), but also with other, non porcine, genetically and antigenically related pestiviruses. Differentiation of infections with CSFV and other pestiviruses is a crucial element of diagnostics. In the present study two real-time PCR methods and conventional one-tube nested PCR for specific detection of CSFV were compared. Additionally, two methods designed for detection of all pestivirus species real-time SYBR Green I and one-tube nested PCR were included into the study. Analyzed methods varied considerably regarding their sensitivity and specificity, what suggests that careful selection of diagnostic methods and their evaluation on a regular basis is necessary.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of antibiotics on acquired immunity in vivo - current state of knowledge
Autorzy:
Pomorska-Mol, M.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/31788.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antibiotic
acquired immunity
in vivo culture
knowledge
immunomodulation
Opis:
Antibiotics are widely used in the therapy of infections. Besides the respective interactions between antibiotics and pathogens it seems that antibiotics also directly interact with the immune system. Some commonly used antibiotics are currently known to have effects on the innate immune response, as shown by in vitro, ex vivo and also in vivo animal experiments and clinical studies. Most of the experimental papers published to date, as well as most reviews, relate to how antibiotics affect the innate immune response or non-specific monocyte or lymphocyte proliferation. However the effects of antibiotics on the adaptive immune response are still not well characterized. This review of the literature considering different in vivo experiments indicate the real importance of interrelations existing between acquired immune responses and antibiotics, however, the mechanism of immunomodulatory effects of antibiotics are still poorly understood. Currently, data on the immunomodulating effects of antibiotics often remain heterogeneous, contradictory or insufficient, but most results published to date revealed the immunosuppressive effect of antibiotics on the antigen- specific immune response in vivo. In pigs as well as in poultry herds, it is not uncommon practice to add antibiotics to drinking water or feed at the time of vaccination. Information on the effects of such practices on the immune system of animals is restricted and more in vivo studies are needed to investigate the effects of antimicrobial drugs on the immune system, especially in the field conditions.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of long-term vaccination of a breeding herd of pigs against PCV2 on reproductive parameters
Autorzy:
Pejsak, Z.
Kusior, G.
Pomorska-Mol, M.
Podgorska, K.
Powiązania:
https://bibliotekanauki.pl/articles/32499.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
long-term vaccination
vaccination
breeding herd
animal breeding
pig
PCV2 virus
reproductive parameter
porcine circoviral disease
circovirus
sow
animal reproduction
reproductive cycle
Opis:
The objective of the study was to evaluate an efficacy of sows vaccination protocols in the herd with serious problems affecting efficacy of reproduction. The study was performed in a large pig herd with about 1200 sows. Before vaccination against PCV2, farrowing rate in this farm was about 65%. Sows, boar and replacement gilts were immunized using Circovac vaccine (Merial, France) according to producer’s recommendations. Parameters of production were analyzed since 2007 until 2010 in selected batches of sows inseminated at the same weeks of the year (17th, 18th, 19th and 20th) to eliminate seasonal variability. In total, 940 sows were subjected to the study. No significant changes in management during these years were introduced. The applied protocol of sow herd long-term vaccination proved to be very efficient. All measured production parameters: reproduction rate, number of piglets born alive, birth weight of piglets and number of piglets weaned per a litter improved after implementation of immunization program. Moreover, further improvement was observed with vaccination in the following reproduction cycles. The most spectacular effect of vaccination regarded average farrowing rate that increased from 64.76% in control group to 86.93% after basic vaccination. Two years after implementation of vaccination program this parameter reached 93.6%. Number of piglets weaned per sow per a litter improved from 10.31 to 11.74 after one year of vaccination and remained relatively stable through the following year. Simultaneously, the percentage of newborn piglets with birth weight < 1 kg decreased significantly (p < 0.05). To summarize, vaccination against PCV2 influenced positively the insemination rate, number of piglets born alive and weaned per litter as well as birth body weight and percentage of piglets weighing < 1 kg.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reactivity of heat-stable Leptospira antigenic preparation used in enzyme-linked immunosorbent assay for detection of antibodies in swine serum
Autorzy:
Wasinski, B.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/31088.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
reactivity
Leptospira
antigen preparation
enzyme-linked immunosorbent
detection
antibody
pig
serum
antigen
animal disease
leptospirosis
laboratory diagnosis
ELISA test
Opis:
Serology plays an important role in laboratory diagnosis of leptospirosis. Apart from the most often used microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA) seems to be useful especially in screenings of animal herds. The ELISA used for detection of antibodies against selected Leptospira serogroups in swine serum samples was investigated during the study. An essential element of this test is heat-stable antigenic preparation from cultures of Leptospira interrogans serovars Icterohaemorrhagiae, Pomona and L. borgpetersenii serovar Sejroe. The aim of the present study was to identify and analyze ELISA heat-stable antigen fractions playing a role in the reaction with leptospiral antibodies indicated in swine serum. Reactivity of the three-component antigenic preparation was compared in immunoblotting with reactivity of six heat-stable antigenic preparations made from the following single serovars: L. interrogans serovars Icterohaemorrhagiae, Pomona, Canicola, L. borgpetersenii serovars Sejroe, Tarassovi and L. kirshneri serovar Grippotyphosa. All antigenic preparations were submitted to SDS-PAGE and transferred to a nitrocellulose membrane using a semidry system. After the transfer, the membrane was incubated with diluted swine serum containing antibodies specific for one of the six above mentioned Leptospira serovars. For the three-component antigenic preparation and antigens prepared from single serovars the immunoblot revealed reaction of sera with fractions of the 20-26 kDa region and around the 14.5 kDa region. The investigated heat-stable Leptospira antigenic preparation contains fractions demonstrating serogroup- and species-specificity. Fraction 20-26 kDa showed serogroup-specific activity, whereas the fraction around 14.5 kDa showed species-specific activity.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł

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