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    Wyświetlanie 1-12 z 12
    Tytuł:
    Studies of plasma interactions with tungsten targets in PF-1000U facility
    Autorzy:
    Ladygina, M. S.
    Skladnik-Sadowska, E.
    Zaloga, D. R.
    Sadowski, M. J.
    Kubkowska, M.
    Kowalska-Strzeciwilk, E.
    Krawczyk, N.
    Paduch, M.
    Miklaszewski, R.
    Garkusha, I. E.
    Powiązania:
    https://bibliotekanauki.pl/articles/147524.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    plasma-surface interaction
    plasma streams
    tungsten
    plasma focus
    PF-1000U
    Opis:
    This paper presents results of experimental studies of tungsten samples of 99.95% purity, which were irradiated by intense plasma-ion streams. The behaviour of tungsten, and particularly its structural change induced by high plasma loads, is of great importance for fusion technology. The reported measurements were performed within a modifi ed PF-1000U plasma-focus facility operated at the IFPiLM in Warsaw, Poland. The working gas was pure deuterium. In order to determine the main plasma parameters and to study the behaviour of impurities at different instants of the plasma discharge, the optical emission spectroscopy was used. The dependence of plasma parameters on the initial charging voltage (16, 19 and 21 kV) was studied. Detailed optical measurements were performed during interactions of a plasma stream with the tungsten samples placed at the z-axis of the facility, at a distance of 6 cm from the electrode outlets. The recorded spectra showed distinct WI and WII spectral lines. Investigation of a target surface morphology, after its irradiation by intense plasma streams, was performed by means of an optical microscope. The observations revealed that some amounts of the electrodes material (mainly copper) were deposited upon the irradiated sample surface. In all the cases, melted zones were observed upon the irradiated target surface, and in experiments performed at the highest charging voltage there were formed some cracks.
    Źródło:
    Nukleonika; 2016, 61, 2; 149-153
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of optical spectra recorded during DPF-1000U plasma experiments with gas-puffing
    Autorzy:
    Zaloga, D. R.
    Skladnik-Sadowska, E.
    Kubkowska, M.
    Ladygina, M. S.
    Malinowski, K.
    Kwiatkowski, R.
    Sadowski, M. J.
    Paduch, M.
    Zielinska, E.
    Makhlaj, V. A.
    Powiązania:
    https://bibliotekanauki.pl/articles/147780.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    electron density
    gas-puff
    optical spectrum
    plasma focus
    plasma stream
    Opis:
    The results are presented of the optical spectra measurements for free plasma streams generated with the use of the modifi ed DPF-1000U machine. This facility was recently equipped with a gas injection system (the so-called gas-puff) placed on the symmetry axis behind the central opening in the inner electrode. The DPF-1000U experimental chamber was fi lled up with pure deuterium at the initial pressure of 1.6 or 2.4 mbar. Additionally, when the use was made of the gas-puff system about 1 cm3 of pure deuterium was injected at the pressure of 2 bars. The gas injection was initiated 1.5 or 2 ms before the triggering of the main discharge. The investigated plasma discharges were powered from a condenser bank charged initially to 23 kV (corresponding to the energy of 352 kJ), and the maximum discharge current amounted to about 1.8 MA. In order to investigate properties of a dense plasma column formed during DPF-1000U discharges the use was made of the optical emission spectroscopy. The optical spectra were recorded along the line of sight perpendicular to the vacuum chamber, using a Mechelle®900 spectrometer. The recent analysis of all the recorded spectra made it possible to compare the temporal changes in the electron density of a freely propagating plasma stream for discharges without and with the gas-puffing. Using this data an appropriate mode of operation of the DPF-1000U facility could be determined.
    Źródło:
    Nukleonika; 2015, 60, 2; 309-314
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena aktywności biologicznej karboksymetylowanych pochodnych alfa-(1→3)-glukanów wyizolowanych z owocników uprawnych gatunków boczniaka (pleurotus)
    Assessing biological activity of carboxymethylated derivatives of alpha-(1→3)-glucans isolated from fruiting bodies of cultivated pleurotus species
    Autorzy:
    Wiater, A.
    Paduch, R.
    Prochniak, K.
    Pleszczynska, M.
    Siwulski, M.
    Bialas, W.
    Szczodrak, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/828789.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Technologów Żywności
    Opis:
    α-(1→3)-Glukany stanowią najmniej poznaną grupę polisacharydów budujących ścianę komórkową grzybów. Celem pracy była ocena aktywności biologicznej karboksymetylowanych pochodnych α-(1→3)-glukanów uzyskanych z owocników uprawnych gatunków rodzaju Pleurotus (P. citrinopileatus, P. djamor, P. erynii i P. precoce). Polisacharydy wyizolowane z owocników poddano analizom strukturalnym (FT-IR i ¹H NMR), w wyniku czego wykazano, że są one liniowymi α-(1→3)-glukanami. Po zastosowaniu metody karboksymetylacji nierozpuszczalne w wodzie α-(1→3)-glukany przeprowadzono w formy rozpuszczalne, tj. karboksymetyl-α-(1→3)-glukany (KM-α-(1→3)-glukany). Następnie analizowano ich wpływ na żywotność komórek prawidłowych CCD 841 CoTr (ludzkie komórki nabłonka jelita grubego) i CCD-18Co (miofibroblasty jelitowe) oraz komórek nowotworowych HeLa (ludzki rak szyjki macicy). Wykonano analizy aktywności metabolicznej i toksyczności (MTT i NR) oraz zdolności do redukcji wolnych rodników (DPPH). Wykazano, że żywotność komórek nowotworowych była najsilniej redukowana przez KM-α-(1→3)-glukan otrzymany z owocników P. citrinopileatus. Z kolei metodą NR wykazano, że wszystkie badane KM-α-glukany w całym zakresie stężeń powodowały statystycznie istotny wzrost żywotności komórek prawidłowych (CCD 841 CoTr i CCD-18Co). Badane KM-α-glukany nie wykazywały aktywności zmierzającej do redukcji wolnych rodników tlenowych. Na podstawie analizy wyników różnicowego barwienia fluorescencyjnego stwierdzono, że KM-α-glukan z owocników P. citrinopileatus zaburza integralność błony komórek nowotworowych. Z kolei barwienie fluorescencyjne filamentów F-aktynowych (F-aktyny) wykazało, że nie wpływał on destrukcyjnie na cytoszkielet badanych komórek.
    α-(1→3)-Glucans constitute the least known group of polysaccharides that make up the fungal cell wall. The objective of the study was to assess the biological activity of carboxymethylated derivatives of α-(1→3)-glucans extracted from fruiting bodies of the cultivated species of Pleurotus (P. citrinopileatus, P. djamor, P. erynii, and P. precoce) genus. The polysaccharides extracted from the fruiting bodies were structurally analyzed (using FT-IR and ¹H NMR) and, based on the analysis results, it was proved that they were linear α-(1→3)-glucans. With the use of a carboxymethylation method, the water-insoluble α-(1→3)-glucans were converted into soluble forms, i.e. carboxymethyl-α-(1→3)-glucans (KM-α-(1→3)-glucans). Next, their effect was investigated on the viability of normal CCD 841 CoTr cells (normal human colonic epithelial cells), CCD-18Co (intestinal myofibroblasts), and HeLa tumour cells (human cervical cancer). The metabolic activity, toxicity (MTT and NR), and free radical scavenging ability (DPPH) were analyzed. It was shown that the effect of KM-α-(1→3)-glucan derived from the P. citrinopileatus fruiting bodies was the strongest as regards the reduction of the cancer cell viability. Then, the NR method used showed that all the tested KM-α-(1→3)-glucans within the entire concentration range caused the viability of normal cells (CCD 841 CoTr and CCD-18Co) to increase statistically significantly. The KM-α-glucans analyzed did not exhibit any free oxygen radical reduction activity. Based on the results of the differential fluorescent staining analysis, it was confirmed that the KM-α-glucan from fruiting bodies of P. citrinopileatus disturbed the membrane integrity of tumour cells. On the other hand, the fluorescent staining of the F-actin filaments proved that this KM-α-glucan did not have any destructive effect on the cytoskeleton of the cells studied.
    Źródło:
    Żywność Nauka Technologia Jakość; 2015, 22, 1
    1425-6959
    Pojawia się w:
    Żywność Nauka Technologia Jakość
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Recent ion measurements within the modified DPF-1000U facility
    Autorzy:
    Kwiatkowski, R.
    Czaus, K.
    Skladnik-Sadowska, E.
    Sadowski, M. J.
    Zaloga, D. R.
    Paduch, M.
    Zielinska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/148249.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    DPF-1000U facility
    ion beams
    ion energy spectra
    ion pinhole images
    Opis:
    In this note we describe measurements of ion beams emitted along the z-axis of the DPF-1000U facility operated at 23 kV, 334 kJ, and with the initial deuterium pressure of 1.6–2 hPa. The DPF-1000U device was recently renewed and equipped with a dynamic gas-puff valve placed inside the inner electrode. The investigated ions were recorded by means of ion pinhole cameras equipped with solid state nuclear track detectors of the PM-355® (PADC) type. The energy spectra of ions were determined using a Thomson spectrometer placed on the symmetry axis at a distance of 160 cm from the electrodes outlets. The ion images recorded during discharges performed under different experimental conditions show that the ion beams have a complex structure, usually in the form of a central bunch and an annular stream composed of many micro-beams. Energies of the registered deuterons have been in the range of 30–700 keV, while the fast protons (which originated from the hydrogen remnants) had energies in the range of 300–850 keV.
    Źródło:
    Nukleonika; 2015, 60, 2; 297-302
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study of tungsten surface interaction with plasma streams at DPF-1000U
    Autorzy:
    Ladygina, M. S.
    Skladnik-Sadowska, E.
    Zaloga, D. R.
    Malinowski, K.
    Sadowski, M. J.
    Kubkowska, M.
    Kowalska-Strzeciwilk, E.
    Paduch, M.
    Zielinska, E.
    Miklaszewski, R.
    Garkusha, I. E.
    Gribkov, V. A.
    Powiązania:
    https://bibliotekanauki.pl/articles/147638.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    DPF-1000U
    electron density
    gas puffing
    plasma stream
    tungsten
    Opis:
    In this note experimental studies of tungsten (W) samples irradiated by intense plasma-ion streams are reported. Measurements were performed using the modified plasma focus device DPF-1000U equipped with an axial gas-puffing system. The main diagnostic tool was a Mechelle®900 optical spectrometer. The electron density of a freely propagating plasma stream (i.e., the plasma stream observed without any target inside the vacuum chamber) was estimated on the basis of the half-width of the Dβ spectral line, taking into account the linear Stark effect. For a freely propagating plasma stream the maximum electron density amounted to about 1.3 × 1017 cm–3 and was reached during the maximum plasma compression. The plasma electron density depends on the initial conditions of the experiments. It was thus important to determine first the plasma flow characteristics before attempting any target irradiation. These data were needed for comparison with plasma characteristics after an irradiation of the investigated target. In fact, spectroscopic measurements performed during interactions of plasma streams with the investigated W samples showed many WI and WII spectral lines. The surface erosion was determined from mass losses of the irradiated samples. Changes on the surfaces of the irradiated samples were also investigated with an optical microscope and some sputtering and melting zones were observed.
    Źródło:
    Nukleonika; 2015, 60, 2; 293-296
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Measurements of electron and ion beams emitted from the PF-1000 device in the upstream and downstream direction
    Autorzy:
    Kwiatkowski, R.
    Skladnik-Sadowska, E.
    Malinowski, K.
    Sadowski, M. J.
    Czaus, K.
    Zebrowski, J.
    Karpinski, L.
    Paduch, M.
    Scholz, M.
    Garkusha, I. E.
    Kubeš, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/146626.pdf
    Data publikacji:
    2011
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    electron beam (EB)
    ion beams
    PF-1000 facility
    magnetic analyzer
    ion pinhole cameras (IPC)
    Opis:
    In this paper we report on measurements of the energy spectra and other properties of the electron and fast ion beams emitted in the upstream and downstream direction along the z-axis of a large plasma focus device PF-1000, that was operated at 21-27 kV, delivering 290–480 kJ pulse. Measurements of the electron beam (EB) properties were performed using a magnetic analyzer. Properties of the ion beams were measured by means of pinhole cameras equipped with PM-355 nuclear track detectors and placed at various angles (0, 60 and 180 centigrade) to the discharge axis. Measurements revealed a complex spatial structure of the fast ion beams. The ion measurements behind the PF-1000 collector proved that some fast deuterons are emitted also in the upstream direction. Measurements of the EBs emitted in the upstream and downstream direction revealed electron energies in the range from approximately 40 keV to approximately 800 keV. These spectra confirm that in localized regions within the PF-1000 plasma column there appear strong fields accelerating charged particles in different directions along the discharge axis.
    Źródło:
    Nukleonika; 2011, 56, 2; 119-123
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nitric oxide (NO) expression in co-culture of colon tumour spheroids with normal cells after incubation with interleukin-1Beta (IL-1Beta) and/or camptothecin (CPT-11)
    Autorzy:
    Paduch, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/3731.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Journal of Pre-Clinical and Clinical Research; 2009, 03, 1
    1898-2395
    Pojawia się w:
    Journal of Pre-Clinical and Clinical Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Clinical usefulness of determining C-reactive protein and fibrinogen concentrations and lipid profile in blood serum of patients undergoing surgery due to atherosclerosis
    Autorzy:
    Niedziela, P.
    Szczepanowski, A.
    Paduch, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/3753.pdf
    Data publikacji:
    2008
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Journal of Pre-Clinical and Clinical Research; 2008, 02, 1
    1898-2395
    Pojawia się w:
    Journal of Pre-Clinical and Clinical Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Essential oil composition and in vitro biological activity of Achillea millefolium L. extracts
    Autorzy:
    Paduch, R.
    Matysik, G.
    Nowak-Kryska, M.
    Niedziela, P.
    Kandefer-Szerszen, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/3131.pdf
    Data publikacji:
    2008
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Journal of Pre-Clinical and Clinical Research; 2008, 02, 1
    1898-2395
    Pojawia się w:
    Journal of Pre-Clinical and Clinical Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    PF-6 an effective plasma focus as a source of ionizing radiation and plasma streams for application in material technology, biology and medicine
    Autorzy:
    Gribkov, V.
    Dubrovsky, A.
    Scholz, M.
    Jednorog, S.
    Karpiński, L.
    Tomaszewski, K.
    Paduch, M.
    Miklaszewski, R.
    Pimenov, V.
    Ivanov, L. I.
    Dyomina, E. V.
    Maslyaev, S. A.
    Orlova, M. A.
    Powiązania:
    https://bibliotekanauki.pl/articles/147424.pdf
    Data publikacji:
    2006
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    dense plasma focus (DPF)
    ion beam
    plasma beam
    X-ray beams
    surface damage
    microlithography
    radioenzymology
    positron emission tomography (PET)
    Opis:
    A review of results on the design and operation of the new efficient Dense Plasma Focus device PF-6 of medium size (transportable) having bank energy of ca. 7 kJ and possessing a long lifetime is presented. New data on the interaction of the pulsed fast ion beams and dense plasma streams generated at this apparatus with various materials are given. These results are compared with the analogous information received at the biggest facility PF-1000. It is shown that it is possible to have about the same power flux density (in the range of 105 109 W/cm2) in both devices however in different areas. Doses of soft X-rays produced by the device within the resists for the goals of microlithography and micromachining appear to be several times less that it is with the conventional X-ray tube. In biological application of this device, medium- and hard-energy X-rays are exploited in the field of radioenzymology. It was found that the necessary dose producing activation/inactivation of enzymes can be by several orders of magnitude lower if used at a high-power flux density in comparison with those received with isotope sources. In medicine, short-life isotope production for the goals of the positron emission tomography (medicine diagnostics) is possible by means of the fast ions generated within DPF. All these experiments are discussed in the framework of pulsed radiation physics and chemistry in its perfect sense thereto the criteria are formulated.
    Źródło:
    Nukleonika; 2006, 51, 1; 55-62
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative analysis of different methodological approaches to the in vitro study of tumour cells chemosensitivity
    Autorzy:
    Paduch, R
    Slotwinska, M.
    Stachura, A.
    Rzeski, W.
    Zdzisinska, B.
    Kandefer-Szerszen, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/961243.pdf
    Data publikacji:
    2001
    Wydawca:
    Uniwersytet Marii Curie-Skłodowskiej. Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej
    Tematy:
    chemosensitivity
    etoposide
    tumour cell
    drug sensitivity
    cisplatin
    gelatin sponge
    growth
    in vitro
    Opis:
    Drug sensitivity assay was performed using two human tumour celi lines: HeLa and Hep-2 cultivated in two-dimensional monolayer celi cultures and three-dimensional cultures on gelatine sponge SpongostanK. Two cytostatics with different mechanisms of anti-tumour action were used: cisplatin and etoposide. Chemosensitivity of tumour cells was assessed by counting the number of viable and nonviable cells (cytostatic and cytotoxic activity of drugs), by counting the number of apoptotic cells and by clonogenic assay of viable cells. We found that the clonogenic assay was morę sensitive than the other tests used, especially after long-term (7 days) treatment of tumour cells with cytostatics. A short (24h) treatment with cytostatics gave false results which were not confirmed after prolonged treatment with cytostatics. We suppose that short treatment tests should not be used for examination of the chemosensitivity of tumour cells isolated from patients. Tumour cells growing on SpongostanH were viable for a longer time than in monolayer cultures and exhibited chemosensitivity comparable to monolayer celi cultures despite of their multilayer growth on gelatine sponge.
    Do badań wrażliwości na cytostatyki użyto dwie ludzkie linie nowotworowe: HeLa i Hep-2, hodowane w formie murawy dwuwymiarowej (płaskiej) oraz w formie przestrzennej, trójwymiarowej, na gąbce żelatynowej Spongostan". W badaniach użyto cytostatyki posiadające różny mechanizm działania przeciwnowotworowego, a mianowicie cisplatynę i etopozyd. Wrażliwość komórek nowotworowych na chemioterapeutyki określano ilością komórek przeżywających i martwych (cytostatyczne i cytotoksyczne właściwości leków), ilością komórek apoptotycznych oraz oceniano klonogenne właściwości komórek przeżywających. Stwierdzono, że ocena klonogennych właściwości komórek jest metodą bardziej czułą w porównaniu z innymi testami, szczególnie po długim (7-dniowym) kontakcie komórek z cytostatykami. Ocena krótkotrwałego (24-godz.) kontaktu komórek nowotworowych z cytostatykami dawała fałszywe wyniki, nie potwierdzone po długotrwałej inkubacji komórek z cytostatykami. Uważamy, że testy polegające na ocenie efektu krótkotrwałej inkubacji komórek z lekami nie powinny być stosowane do oceny wrażliwości na chemioterapeutyki komórek nowotworowych izolowanych od pacjenta. Komórki nowotworowe rosnące na Spongostanie” były żywe dłużej niż rosnące w hodowlach płaskich i pomimo wielowarstwowego wzrostu na gąbce żelatynowej wykazywały wrażliwość na leki porównywalną z hodowlami płaskimi.
    Źródło:
    Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia; 2001, 56
    2083-3563
    0066-2232
    Pojawia się w:
    Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Recent progress in 1 MJ Plasma-Focus research
    Autorzy:
    Scholz, M.
    Karpiński, L.
    Paduch, M.
    Tomaszewski, K.
    Miklaszewski, R.
    Szydlowski, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/147553.pdf
    Data publikacji:
    2001
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    neutron emission
    plasma focus
    scaling laws
    soft X-ray radiation
    Opis:
    The paper reports on actual operational characteristics of the PF-1000 facility and it presents results of the first neutron experiments performed with a new set of large Mather-type electrodes, which were designed for shots up to 1000 kJ. Dynamics of a current-sheath was studied by means of two high-speed streak cameras and a two-frame camera. The last one was used for the side-on observation of the pinch column. The high-speed streak pictures were taken side-on behind slits oriented in the radial or axial direction. On the basis of the pictures taken behind the radial slit, average values of the radial compression and expansion velocities were estimated. Time-resolved X-ray signals were measured with PIN diodes covered by different filters, and they were compared with other traces (voltage waveforms, dI/dt signals, and neutron-induced pulses) in order to determine their correlation. The total neutron yield (Ytot), i.e. the number of neutrons produced during a single discharge and emitted in various directions, was measured by means of several silver-activation detectors placed at different angles around the PF-1000 experimental chamber.
    Źródło:
    Nukleonika; 2001, 46, 1; 35-39
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-12 z 12

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