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Wyszukujesz frazę "Lin, X.Y." wg kryterium: Autor

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    Wyświetlanie 1-14 z 14
    Tytuł:
    A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
    Autorzy:
    Hu, L.
    Lin, X.Y.
    Yang, Z.X.
    Yao, X.P.
    Li, G.L.
    Peng, S.Z.
    Wang, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/30249.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A key metric and its calculation models for a continuous diagnosis capability based on a dependency matrix
    Autorzy:
    Shi, J.-Y.
    Lin, X.-G.
    Shi, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/221741.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    fault diagnosis
    dependency matrix
    measurement
    Opis:
    This paper is devoted to measuring the continuous diagnosis capability of a system. A key metric and its calculation models are proposed enabling us to measure the continuous diagnosis capability of a system directly without establishing and searching the sequential fault tree (SFT) of the system. At first a description of a D matrix is given and its metric is defined to determine the weakness of a continuous diagnosis. Then based on the definition of a sequential fault combination, a sequential fault tree (SFT) is defined with its establishment process summarized. A key SFT metric is established to measure the continuous diagnosis capability of a system. Two basic types of dependency graphical models (DGMs) and one combination type of DGM are selected for characteristics analysis and establishment of metric calculation models. Finally, both the SFT searching method and direct calculation method are applied to two designs of one type of an auxiliary navigation equipment, which shows the high efficiency of the direct calculation method.
    Źródło:
    Metrology and Measurement Systems; 2012, 19, 3; 509-520
    0860-8229
    Pojawia się w:
    Metrology and Measurement Systems
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study of flow softening mechanisms of a nickel-based superalloy with o phase
    Autorzy:
    Lin, Y. C.
    He, D.-G
    Chen, M. S.
    Chen, X.-M.
    Zhao, C.-Y.
    Ma, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/958203.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    alloys
    hot deformation
    flow softening behavior
    microstructural evolution
    Opis:
    The flow softening behaviors of a nickel-based superalloy with o phase are investigated by hot compression tests over wide ranges of deformation temperature and strain rate. Electron backscattered diffraction (EBSD). optical microscopy (OM), and scanning electron microscopy (SEM) are employed to study the flow softening mechanisms of the studied superalloy. It is found that the flow softening behaviors of the studied superalloy are sensitive to deformation temperature and strain rate. At high strain rate and low deformation temperature, the obvious flow softening behaviors occur. With the increase of deformation temperature or decrease of strain rate, the flow softening degree becomes weaken. At high strain rate (1s-1), the flow softening is mostly induced by the plastic deformation heating and flow localization. However, at low strain rate domains (0.001-0.01s-1), the effects of deformation heating on flow softening are slight. Moreover, the flow softening at low strain rates is mainly induced by the discontinuous dynamic recrystallization and the dissolution of 6 phase (Ni3Nb).
    Źródło:
    Archives of Metallurgy and Materials; 2016, 61, 3; 1537-1546
    1733-3490
    Pojawia się w:
    Archives of Metallurgy and Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein
    Autorzy:
    Wu, X.
    Xiao, L.
    Peng, B.
    Wang, Y.
    Yang, Z.
    Yao, X.
    Hu, L.
    Lin, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/30963.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antioxidant activity and mechanism in flower of Hylocereus undatus (Haw.) Britt. et Rose
    Autorzy:
    Li, X.
    Gao, Y.
    Han, W.
    Lin, J.
    Hu, Q.
    Chen, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/19838.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Hylocereus undatus flower is commonly used as food or for medicinal purposes in south China. To study its antioxidant activity and mechanism we used antioxidant and chemical assays to compare two commercial samples from different locations (Shenjing, Qixing). The difference in antioxidant levels corresponded with differences in chemical content (including total phenolics, total flavonoids, kaempferol and quercetin) between Shenjing and Qixing. The antioxidant ability of H. undatus flower seems attributable to total phenolics (mainly total flavonoids). Kaempferol is one of the main bioactive components. H. undatus flower exerts its antioxidant effects through metal chelation and radical scavenging via hydrogen atom (H•) and electron (e) donation.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2013, 55, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    CO2 concentration affects in vitro pig embryo developmental capacity
    Autorzy:
    Zhang, L.
    Lin, Z.
    Bi, Y.
    Zheng, X.
    Xiao, H.
    Hua, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087657.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    CO2
    developmental capacity
    pig embryos
    in vitro
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 609-614
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Trace muscovite dissolution separation from vein quartz by elevated temperature and pressure acid leaching using sulphuric acid and ammonia chloride solutions
    Autorzy:
    Lin, M.
    Pei, Z.-Y.
    Lei, S.-M.
    Liu, Y.-Y.
    Xia, Z.-J.
    Xie, F.-X.
    Powiązania:
    https://bibliotekanauki.pl/articles/110755.pdf
    Data publikacji:
    2018
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    vein quartz
    muscovite
    sulphuric acid
    ammonium chloride
    acid leaching
    Opis:
    Effects of sulphuric acid and ammonia chloride on muscovite dissolution were studied in acid leaching of vein quartz under elevated temperature and pressure. The leaching processes have been studied in detail by analyzing sources of impurity minerals, optimizing leaching process, analyzing leaching kinetics of Al in muscovite and charactering leaching mechanism of muscovite. The results showed that elements of Al and K mainly occurred in muscovite, and 98.10% or more of muscovite could be removed by acid leaching, while the process had limited influence on the particle size of quartz sand. Leaching of Al in the quartz ore was mainly controlled by chemical reaction. A calcination process and ammonia chloride were used for reducing chemical reaction resistance by damaging crystal structure of muscovite and providing stable acid leaching environment. Combined with the calcination process, muscovite, as a main gangue mineral, was effectively extracted during acid leaching of vein quartz at elevated temperature and pressure.
    Źródło:
    Physicochemical Problems of Mineral Processing; 2018, 54, 2; 448-458
    1643-1049
    2084-4735
    Pojawia się w:
    Physicochemical Problems of Mineral Processing
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Seasonal dynamics in cambial activity and the formation of xylem and phloem in the branches of Cinnamomum camphora
    Autorzy:
    Dong, M.
    Xu, Y.M.
    Lin, H.
    Li, X.Q.
    Xia, Q.Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/41385.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    seasonal dynamics
    cambial activity
    formation
    xylem
    phloem
    branch formation
    tree
    tropical tree
    Cinnamomum camphora
    wood formation
    phenology
    Opis:
    The cambial activity and periodicity of secondary xylem and phloem formation have been less studied in tropical tree species than in temperate ones. Cambium activity is the only source of timber production. Studies on its activity and xylem formation can provide helpful data for dendrochronology and possible factors that control tree growth, the yield and quality of wood. Cinnamomum camphora is an important fast-growing tree for furniture and sculpture wood in South China. Its dynamics of cambial activity was not reported. During the growth season in 2011, five trees of Cinnamomum camphora plantation were monitored on the campus of Huazhong Agricultural University in central china (located on 113°41’ to 115°05’ E and 29°58’ to 31°22’ N). Sampling time was from 13, February 2011 to 13, February 2012. Some small stems of 15–20 cm length were selected to sample in the middle of 2–3 years old health branches in the central crown of these trees. The observational study found that the cambium of camphor trees was active from March to October and dormant from November to February. Onset of cambial cell division was observed in early March, one week after sprouting of new buds. The morphology of the cells and number of cell layers in the cambium zone varied throughout the year. In early March, cambium reactivated with the rising of temperature, the cell wall was relative thick. The number of cell layers increased from 2–3 in middle Feb. to 3–5. In June, the number of cell layers in cambium reached the maximum of 5–7 in a radial direction. In mid March, phloem cells began to differentiate, followed by xylem three weeks later. The number of cell layers in immature phloem and xylem increased dramatically before mid June and the increment of cell layers in xylem was almost fifth times of phloem in the differentiating process. The phloem cells mainly stopped dividing new cells in end of October, but xylem cells were still in the activity a week later. The annual activities of cambium was in accordance with the air mean temperature change in 2011–2012. The results showed that the cambial activity and formation of xylem and phloem were related to the environmental factors, especially the temperature.
    Źródło:
    Dendrobiology; 2016, 75
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of TaqMan-based real-time PCR assay based on the E1 genefor the quantitative detection of the Getah virus
    Autorzy:
    Lin, A.
    Hu, X.
    Cui, S.
    Yang, T.
    Zhang, Z.
    Li, P.
    Guo, M.
    Lu, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647453.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    Getah virus
    real-time PCR
    TaqMan
    detection
    Opis:
    To develop a sensitive, specific, and rapid approach for the detection Getah virus (GETV), a set of primers targeting the conserved region of the E1 gene was created. The TaqMan-based real-time PCR method for GETV detection was developed by optimizing the reaction conditions. The method demonstrated excellent specificity, and amplification did not occur with the causative agents of all prevalent swine viral infections (CSFV, PRRSV, PRV, PEDV, PTV, and JEV), except GETV. Additionally, upon assessing the sensitivity of the method, the minimum detection limit for GETV was found to be 5.94 copies/μL, which is 10 times higher than that of the traditional PCR approach. Further, the intra- and inter-assay variation coefficients were less than 1%, demonstrating good repeatability. Moreover, GETV was found in 10 of the 20 field serum samples using real-time PCR but only in three of the samples using traditional PCR. Consequently, the first GETV TaqMan-based real-time PCR approach based on the E1 gene was developed for GETV pathogenic diagnoses, and this exhibited high specificity, sensitivity, and repeatability. This assay is practical for the pathogenic diagnosis and epidemiology of GETV.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 1; 21-28
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dft investigation of a high energy density polynitro compound, 2,2’-Bis(trinitromethyl)-5,5’-azo-1,2,3,4- tetrazole
    Autorzy:
    Lin, H.
    Zhu, S. G.
    Chen, P. Y.
    Li, K.
    Li, H. Z.
    Peng, X. H.
    Powiązania:
    https://bibliotekanauki.pl/articles/358030.pdf
    Data publikacji:
    2013
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    polynitro
    electronic structure
    thermodynamic properties
    crystal structure
    detonation performance
    stability
    Opis:
    A novel polynitro compound, 2,2’-bis(trinitromethyl)-5,5’-azo- 1,2,3,4-tetrazole, was designed and investigated at the DFT-B3LYP/6-31G(d) level. Its properties, such as electronic structure, IR spectrum, heat of formation, thermodynamic properties and crystal structure, were predicted. This compound is most likely to crystallize in the P21 space group, and the corresponding cell parameters are Z = 2, a = 5.46 Å, b = 9.72 Å, c = 14.05 Å, α = 90°, β = 90°, γ = 90°. In addition, the detonation velocity and pressure were also estimated by using the empirical Kamlet-Jacobs equations, and were predicted to be 8.28 km/s and 31.61 GPa respectively. The oxygen balance of this compound is +13.79%, which indicates that it could serve as an oxidizer. Bond dissociation energy calculations show that the C(13)-N(21)O2 and C(14)-N(30)O2 bonds are the locations of thermal decomposition and that this compounds meets the thermal stability requirements as an exploitable explosive. Keywords: polynitro, electronic structure, thermodynamic properties, crystal structure, detonation performance, stability.
    Źródło:
    Central European Journal of Energetic Materials; 2013, 10, 3; 325-338
    1733-7178
    Pojawia się w:
    Central European Journal of Energetic Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A new adaptation for in vitro co-culture of single porcine parthenogenetic embryos using feeder cells
    Autorzy:
    Zhang, L.
    Lin, Z.
    Hua, Z.
    Zheng, X.
    Xiao, H.
    Hua, W.
    Ren, H.
    Zhu, Z.
    Molenaar, A.
    Bi, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087368.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    feeder cells
    porcine
    embryos
    in vitro
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 4; 711-716
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation and sequence analysis of the complete VP2 gene of canine parvovirus from Chinese domestic pets and determination of the pathogenesis of these circulating strains in beagles
    Autorzy:
    Chen, M.R.
    Guo, X.Y.
    Wang, Z.Y.
    Jiang, Y.T.
    Yuan, W.F.
    Xin, T.
    Hou, S.H.
    Song, T.Q.
    Lin, W.D.
    Zhu, H.F.
    Jia, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087542.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    canine parvovirus
    molecular epidemiology
    phylogenetic analysis
    pathogenesis
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 2; 287-296
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The distribution of different virulence grass carp reovirus strains in some neglected tissues
    Autorzy:
    Liang, H.R.
    Fu, X.Z.
    Li, N.Q.
    Liu, L.H.
    Lin, Q.
    Li, Y.G.
    Peng, Y.A.
    Huang, Z.B.
    Wu, S.Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/30668.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-14 z 14

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