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Wyświetlanie 1-15 z 26
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in nutrient and heavy metal content levels of sawdust due to vermicomposting by Allomyrina dichotoma subsp. tunobosonis
Autorzy:
Hoang, C.N.
Yang, Z.-W.
Huang, M.-Y.
Lin, K.-H.
Yang, C.-M.
Nguyen, M.K.
Powiązania:
https://bibliotekanauki.pl/articles/81229.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
vermicomposting
organic waste
nutrient
heavy metal content
sawdust substrate
Allomyrina dichotoma
beetle
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Construction of a recombinant baculovirus expressing swine hepatitis E Virus ORF2 and preliminary research on its immune effect
Autorzy:
Yang, Z.
Wang, K.
Hu, Y.
Yuan, P.
Yang, Y.
Xie, L.Y.
Huang, S.L.
Liu, J.
Ran, L.
Song, Z.H.
Powiązania:
https://bibliotekanauki.pl/articles/2087759.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
swine HEV
ORF2
baculovirus
immunogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 47-54
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
Autorzy:
Xu, T.
Huang, X.
Li, Y.
Liu, Q.
Liu, X.
Han, K.
Liu, Y.
Yang, J.
Zhao, D.
Bi, K.
Sun, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087719.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
goose-derived
duck-derived
chicken-derived
Tembusu virus
pathogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of gas holdup on the efficiency of cyclonic-static microbubble flotation column for oily wastewater treatment
Autorzy:
Ning, K.
Huang, G.
Xu, H.
Wu, L.
Li, X.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/208139.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
bubble columns
efficiency
flotation
wastewater treatment
oil water separation
kolumna flotacyjna
efektywność
flotacja
oczyszczanie ścieków
separacja wody
Opis:
A cyclonic-static microbubble flotation column of a novel construction was used in oil-water separation fields and has high efficiency for oil-water separation. The gas holdup is a key parameter for the evaluation of the performance of a flotation column. The gas holdup, closely related to the bubble size, bubble velocity and superficial gas velocity, is one of the most important parameters characterizing the hydrodynamics of a bubble column. The effect of gas holdup in a cyclonic-static microbubble flotation column was investigated. In addition, several operating parameters such as the circulating pressure, superficial gas velocity, and frother consumption were also investigated. The gas holdup was positively correlated to the superficial gas velocity. The gas holdup of clean water and oil wastewater increased along with the increase of the frother consumption. The separation mechanism of cyclonic-static microbubble flotation column was analyzed.
Źródło:
Environment Protection Engineering; 2018, 44, 2; 5-17
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Vacuum Hot-Press Process on The Sintered Characteristics and Mechanical Properties of A High-Density Cr-31.2 Mass% Ti Alloy
Autorzy:
Chang, S.-H.
Li, C.-L.
Huang, K.-T.
Yang, T.-H.
Powiązania:
https://bibliotekanauki.pl/articles/356911.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Cr-31.2 mass% Ti alloy
vacuum hot-press sintering
relative density
TRS
electrical conductivity
Opis:
In this study, two different compositions of submicron-structured titanium (760 nm) and micron-structured chromium (4.66 μm) powders were mixed to fabricate Cr-31.2 mass% Ti alloys by vacuum hot-press sintering. The research imposed various hot-press sintering pressures (20, 35 and 50 MPa), while the sintering temperature maintained at 1250°C for 1h. The experimental results showed that the optimum parameters of the hot-press sintered Cr-31.2 mass% Ti alloys were 1250°C at 50 MPa for 1h. Also, the relative density reached 99.94%, the closed porosity decreased to 0.04% and the hardness and transverse rupture strength (TRS) values increased to 81.90 HRA and 448.53 MPa, respectively. Moreover, the electrical conductivity is enhanced to 1.58 × 104 S·cm-1 However, the grain growth generated during the high-temperature and high-pressure of the hot-press sintering process resulted in the grain coarsening phenomenon of the Cr-31.2 mass% Ti alloys after 1250°C hot-press sintering at 50 MPa for 1h. In addition, the Cr-31.2 mass% Ti alloys were fabricated with the submicron-structured titanium (760 nm) and chromium (588 nm) powders showed more effective compaction than the micron-structured titanium (760 nm) and chromium (4.66 μm) powders did. The closed porosity decreases to 0.02% and the hardness values increase to 83.23 HRA. However, the agglomeration phenomenon of the Cr phase and brittleness of the TiCr2 Laves phases easily led to a slight decrease in TRS (400.54 MPa).
Źródło:
Archives of Metallurgy and Materials; 2018, 63, 4; 1715-1723
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotyping and pathogenic characterization of canine distemper virus based on mutations in the hemagglutinin gene in Chinese domestic dogs
Autorzy:
Chen, M.
Xin, T.
Hou, S.
Lin, W.
Song, W.
Zhu, H.
Huang, K.
Jia, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087680.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
canine distemper virus
hemagglutinin
phylogenetic analysis
animal infection
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 623-629
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kinetic research of quinoline, pyridine and phenol adsorption on modified coking coal
Autorzy:
Sun, X.
Xu, H.
Wang, J.
Ning, K.
Huang, G.
Yu, Y.
Ma, L.
Powiązania:
https://bibliotekanauki.pl/articles/110365.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
adsorption kinetics
modification
coking coal
coking wastewater
Opis:
Adsorption is widely used in wastewater treatment. In this work, the removal of quinoline, pyridine and phenol from coking wastewater by using modified coking coal, which was treated by four different modification methods i.e. acidification sodium hydroxide (5 mol/dm3), hydrochloric acid (5 mol/dm3) and acetic acid (5 mol/dm3) and low-temperature (105 oC) oxidation, was investigated. The modified coal was characterized by the surface area analysis, SEM, total acidity and basicity and FT-IR. The results showed that the surface area from high to low follows the order: modification with acetic acid, modification with hydrochloric acid, raw coal, modification with sodium hydroxide and modification with low-temperature. Experimental data were fitted to pseudo-first-order, pseudo-second-order and intra-particle diffusion. The adsorption of all followed pseudo-second-order kinetics. The result showed that the removal efficiency of coal modified by hydrochloric acid and acetic acid are higher than raw coal, while modified by sodium hydroxide and low-temperature are lower than raw coal., The coal modified by hydroxide acid had the best adsorption capacity.
Źródło:
Physicochemical Problems of Mineral Processing; 2018, 54, 3; 965-974
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microstructure and Impact Toughness of Offshore Steel
Autorzy:
Cheng, T.-C.
Yu, C.
Yang, T.-C.
Huang, C.-Y.
Lin, H.-C.
Shiue, R.-K.
Powiązania:
https://bibliotekanauki.pl/articles/351264.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
low-temperature impact toughness
prior austenite grain size
hardenability
bainite
Opis:
This study focused on the effects of microstructures on the low-temperature impact toughness of directly water-quenched offshore steel. Martensite dominated the microstructure directly below the quenched surface. In contrast, mainly lower bainite, martensite, and low amount of ferrite were observed in the central region of the quenched specimen. The ductile to brittle transition temperature of tempered martensite was significantly lower than that of a bainite-dominated microstructure. It is found that low-angle boundaries within the bainite packets greatly impair the low-temperature impact toughness of the steel. The absence of high-angle boundaries in the lower bainite packets significantly deteriorates the low-temperature impact energy of offshore steel.
Źródło:
Archives of Metallurgy and Materials; 2018, 63, 1; 167-172
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie nowoczesnych cieczy roboczych w chwytakach robotów
Autorzy:
Osowski, K.
Fernández, M. A.
Olszak, A.
Huang, C. Y.
Kęsy, A.
Chang, J. Y.
Kęsy, Z.
Powiązania:
https://bibliotekanauki.pl/articles/305102.pdf
Data publikacji:
2018
Wydawca:
Wydawnictwo Druk-Art
Tematy:
chwytaki
sprzęgła wiskotyczne
ciecz elektroreologiczna
ciecz magnetoreologiczna
grasper
viscous coupling
electro-medical liquid
magnetorheological fluid
Opis:
W artykule omówiono wykorzystanie cieczy o nietypowych właściwościach w chwytakach robotów. Ciecze elektro- i magnetoreologiczne, zmieniające swoje właściwości reologiczne w obecności pola elektrycznego lub magnetycznego, zastosowano jako ciecze robocze sprzęgieł wiskotycznych, wchodzących w skład układu przeniesienia napędu chwytaków. Przedstawiono dwa rozwiązania konstrukcyjne prototypowych chwytaków robotów przemysłowych z takimi sprzęgłami. Rozważono również ciecze luminescencyjne, reagujące na nacisk, pod kątem ich zastosowania w mikroczujnikach siły nacisku szczęk chwytaka.
The paper discusses the use of modern fluids with untypical properties in robot grippers. The electro- and magnetorheological fluids, changing their rheological properties in the presence of an electric or magnetic field, were used as working fluids of viscous clutches included in the griper drive transmission system. Two design solutions were presented for prototype industrial robot grippers with such clutches. Luminescent pressure-sensitive fluids have also been considered for their use in the micro-sensors for sensing force of the gripper’s jaw.
Źródło:
Napędy i Sterowanie; 2018, 20, 3; 100-106
1507-7764
Pojawia się w:
Napędy i Sterowanie
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Design, preparation and properties of novel flame retardant thermosetting vinyl ester copolymers based on castor oil and industrial dipentene
Autorzy:
Mao, W.
Li, S.
Li, M.
Huang, K.
Xia, J.
Powiązania:
https://bibliotekanauki.pl/articles/777956.pdf
Data publikacji:
2017
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
castor oil
vinyl ester resin
copolymers
flammability
thermal properties
Opis:
A novel bio-based flame-retardant thermosetting vinyl ester resin monomer was synthesized from castor oil. The chemical structures of the monomer was characterized by FTIR and 1H-NMR. In order to improve its rigidity and expand its application in the field of bio-based materials, it was mixed with certain proportions of another reactive bio-based VER monomer, which had rigid and strong polar groups, and then a series of copolymers were prepared with thermal curing method. Then their tensile property, hardness, morphology of fractured surface, flame retardant property, DMA and thermostability were all investigated. The results indicated that the copolymers had relatively high tensile strength of 11.2 MPa, and the limiting oxygen index is above 23% in all prepared copolymers. DMA demonstrates that the glass transition temperature of the cured resins is up to 56.1°C. Thermogravimetric analysis shows that the copolymers have excellent thermal stability.
Źródło:
Polish Journal of Chemical Technology; 2017, 19, 3; 1-8
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of preparation methods of mixed calcium and zinc thermal stabilizers derived from dimer fatty acid and tung-oil based C22 triacid on properties of PVC
Autorzy:
Li, M.
Wang, M.
Li, S.
Huang, K.
Mao, W.
Xia, J.
Powiązania:
https://bibliotekanauki.pl/articles/778123.pdf
Data publikacji:
2017
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
poly(vinyl chloride)
mixed calcium and zinc thermal stabilizer
synthesis technology
thermal stabilization
Opis:
Calcium and zinc salts of dimer fatty acids (DFA-Ca and DFA-Zn) were synthesized using direct neutralization and metathesis technologies, respectively. The adduct of maleic anhydride and methyl eleostearate (MAME) was also converted to the corresponding zinc soap (C22TA-Zn) and calcium soap (C22TA-Ca) by the two different synthetic routes. Mixed Ca/Zn salts between DFA-Ca and DFA-Zn, and between C22TA-Zn and C22TA-Ca were used as thermal stabilizers for poly(vinyl chloride) (PVC). The PVC thermal stability was determined using Congo red test, discoloration test, torque rheological analysis and TGA. Dynamic mechanical properties were also tested. Results indicated that the DFA-Ca/DFA-Zn thermal stabilizer from direct neutralization technology was found to be superior to that of the metathesis product. The C22TA-Ca/C22TA-Zn thermal stabilizer from direct neutralization method had overall superior thermal stability, and displayed modulus and glass transition comparable to that of metathesis product. Direct neutralization method was more excellent and convenient than metathesis technology.
Źródło:
Polish Journal of Chemical Technology; 2017, 19, 2; 78-87
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Potato waste treatment by microbial fuel cell. Evaluation based on electricity generation, organic matter removal and microbial structure
Autorzy:
Du, H.
Li, F.
Huang, K.
Li, W.
Feng, C.
Powiązania:
https://bibliotekanauki.pl/articles/206898.pdf
Data publikacji:
2017
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
water treatment
performance
technology
bacterial
recovery
sludge
carbon
microbial fuel cell
COD
potato waste
uzdatnianie wody
ChZT
węgiel
odpady ziemniaczane
Opis:
The performance of microbial fuel cell (MFC) in treating potato waste was evaluated using a two-chamber MFC supplied with potato liquid after mastication of market available fresh potato. Evaluation was conducted based on electricity generation, organic matter removal (CODCr, DOC and volatile fatty acids (VFAs)), and microbial structure on the anode and in the anodic solution of the reactor. Current density exhibited a trend that followed the concentration changes of organic matter in the solution, with its highest value being observed as 208 mA/m2. Effective removal of organic matter was also observed. By the end of the experiment, the removal for total COD reached about 84%. Bacterial structure analysis based on PCR, DGGE and sequencing indicated that more species were developed in the anodic solution than on the anode, with Proteobacteria, Firmicutes and Bacteroides being dominant. Geobacter, a well reported exoelectrogenic species, was found more predominant on the anode than in the anodic solution. The results thus indicated that simultaneous stabilization and electricity generation could be achieved when potato waste is treated in MFC.
Źródło:
Environment Protection Engineering; 2017, 43, 1; 5-18
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
Autorzy:
Han, K.
Zhao, D.
Liu, Y.
Liu, Q.
Huang, X.
Yang, J.
Bi, K.
Xu, T.
Li, Y.
Powiązania:
https://bibliotekanauki.pl/articles/31495.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 26

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