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    Wyświetlanie 1-6 z 6
    Tytuł:
    Use of fluorescence quantitative polymerase chain reaction (PCR) for the detection of Escherichia coli adhesion to pig intestinal epithelial cells
    Autorzy:
    Dai, C.H.
    Gan, L.N.
    Qin, W.U.
    Zi, C.
    Zhu, G.Q.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31510.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    An efficient and accurate method to test Escherichia coli (E. coli) adhesion to intestinal epithelial cells will contribute to the study of bacterial pathogenesis and the function of genes that encode receptors related to adhesion. This study used the quantitative real-time polymerase chain reaction (qPCR) method. qPCR primers were designed from the PILIN gene of E. coli F18ab, F18ac, and K88ac, and the pig β-ACTIN gene. Total deoxyribonucleic acid (DNA) from E. coli and intestinal epithelial cells (IPEC-J2 cells) were used as templates for qPCR. The 2−ΔΔCt formula was used to calculate the relative number of bacteria in cultures of different areas. We found that the relative numbers of F18ab, F18ac, and K88ac that adhered to IPEC-J2 cells did not differ significantly in 6-, 12-, and 24-well culture plates. This finding indicated that there was no relationship between the relative adhesion number of E. coli and the area of cells, so the method of qPCR could accurately test the relative number of E. coli. This study provided a convenient and reliable testing method for experiments involving E. coli adhesion, and also provided innovative ideas for similar detection methods.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The Research on Characterization of Crushability for Foundry Sand Particles
    Autorzy:
    Dai, Y.
    Ma, Q. Y.
    Li, X. H.
    Zhang, X.
    Hu, F. P.
    Zhang, Y.
    Xie, W. D.
    Powiązania:
    https://bibliotekanauki.pl/articles/379689.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    foundry sand particle
    mechanical load
    AFS grain fineness
    crushability
    piasek odlewniczy
    obciążenie mechaniczne
    rozdrobnienie ziarna
    Opis:
    In this paper, crushability of foundry sand particles was studied. Three kinds of in-service silica sands in foundry enterprises selected as the study object, and foundry sand particles were subjected to mechanical load and thermal load during service were analyzed. A set of methods for simulating mechanical load and thermal load by milling and thermal-cold cycling were designed and researched, which were used to characterize the crushability for silica sand particles, the microstructure was observed by SEM. According to the user’s experience in actual application, the crushability of Sand C was the best and then Sand B, the last Sand A. The results indicated that mechanical load, thermal load and thermal-mechanical load can all be used to characterize the crushability of foundry sand particles. Microscopic appearances can qualitatively characterize the crushability of foundry sand particles to a certain extent, combining with the additions and cracks which are observed on the surface.
    Źródło:
    Archives of Foundry Engineering; 2017, 17, 4; 231-235
    1897-3310
    2299-2944
    Pojawia się w:
    Archives of Foundry Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
    Autorzy:
    Dai, G.
    Huang, X.
    Liu, Q.
    Li, Y.
    Zhang, L.
    Han, K.
    Yang, J.
    Liu, Y.
    Xue, F.
    Zhao, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647377.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    epitope
    goose astrovirus
    capsid protein
    monoclonal antibody
    Opis:
    Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fast and effective extraction for equivalent shunt resistances of triple-junction concentrator solar cells
    Autorzy:
    Lv, H
    Dai, J
    Sheng, F
    Liu, W
    Ma, X
    Cheng, C
    Lv, Q
    Powiązania:
    https://bibliotekanauki.pl/articles/174425.pdf
    Data publikacji:
    2015
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    concentrator photovoltaic
    triple junction solar cell
    equivalent shunt resistance
    Opis:
    Fast and effective extraction of equivalent shunt resistance for each subcell of GaInP/GaInAs/Ge triple-junction concentrator solar cells is presented. The two-diode model of single junction was introduced to establish the equivalent circuit of triple-junction solar cells. The current-voltage characteristic of the triple-junction solar cells was measured under AM1.5D spectrum, C = 576 and T = 303 K. Equivalent shunt resistance of each subcell was extracted from its estimated current-voltage curve. The estimated current-voltage curve of the triple-junction solar cells shows a good agreement with the experimental data in 0.31% deviation. The degradation in the equivalent shunt resistance for Ge subcell was intentionally introduced to indicate the mechanism of current-matching operation for different subcells, with the maximum output power of the triple-junction solar cells deteriorating from 3.5 to 3.17 W. The results can offer performance analysis and optimum design of photovoltaic applications.
    Źródło:
    Optica Applicata; 2015, 45, 2; 227-235
    0078-5466
    1899-7015
    Pojawia się w:
    Optica Applicata
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Experimental study on thermo physical parameter of loose coal bulk
    Badania eksperymentalne parametrów termofizycznych luźnych brył węgla
    Autorzy:
    Chen, Q. H.
    Dai, G. L.
    Zhang, G. S.
    Fan, X. Q.
    Qin, R. X.
    Powiązania:
    https://bibliotekanauki.pl/articles/348819.pdf
    Data publikacji:
    2012
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Wydawnictwo AGH
    Tematy:
    parametry termofizyczne
    luźny węgiel
    badania eksperymentalne
    współczynniki wpływu
    thermo-physical parameters
    loose coal
    experimental study
    influence factors
    Opis:
    Experimental study was carried out on the thermo physical parameter of loose coal bulk, based on hot-wired method, the relationship between thermo physical parameter of loose coal bulk and each influence factors obtained, and the result shows that the thermal conductivity increased and thermal capacity decreased on condition that the coal rank increased, with the increasing of water content, thermal conductivity and thermal capacity are both have the trend of ascent. Within the experimental temperature limit (< 110 degrees of Celsius), thermal conductivity shows linear growth trend with the increasing of temperature. When voidage increased, thermal conductivity decreased, but the relevance between thermal capacity and voidage is unnoticeable.
    Wyniki badań eksperymentalnych przeprowadzonych w celu określenia parametrów termofizycznych luźnych brył węgla metodą "gorącej nici" (ang. hot-wire method) i zależności pomiędzy parametrem termofizycznym luźnych brył węgla a każdym z uzyskanych współczynników wpływu pokazują wzrost przewodności cieplnej i obniżenie pojemności cieplnej wraz z poprawą jakości węgla. Przy zwiększaniu zawartości wody przewodność cieplna i pojemność cieplna wykazują tendencje wzrostowe. W badanym zakresie temperatur (< 110 stopni Celsjusza) przewodność cieplna wzrasta liniowo wraz ze wzrostem temperatury. Zwiększenie przestrzeni pomiędzy elementami skutkowało obniżeniem przewodności cieplnej, jednak nie zaobserwowano związku pomiędzy pojemnością cieplną a większą przestrzenią między elementami.
    Źródło:
    AGH Journal of Mining and Geoengineering; 2012, 36, 3; 89-98
    1732-6702
    Pojawia się w:
    AGH Journal of Mining and Geoengineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cell wall-degrading enzymes and aggressiveness in Stagonopspora nodorum
    Autorzy:
    Cui, K. R.
    Krupinsky, J. M.
    Dai, Q.
    Arseniuk, E.
    Ueng, P. P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2198866.pdf
    Data publikacji:
    2005-06-23
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    aggressiveness
    β-1,3-glucanase
    cellulase
    isozymes
    isoelectric focusing
    pectinase
    secretion
    Stagonospora nodorum
    xylanase
    Opis:
    Stagonospora nodorum produces cell wall degrading enzymes when grown in culture media containing cell wall components. The pathogen grew as well on minimal agar plates containing cellulose, xylan and pectin as glucose, except having sparser mycelia. Four cell wall-degrading enzymes, cellulase, xylanase, pectinase and b-1,3-glucanase were coordinately induced in culture filtrates growing on xyaln and cellulose as substrates. An aggressive isolate (sn26-1) secreted more cell wall-degrading enzymes than the others. Based on isoelectric focusing profiles, six to seven xylanase isozymes were induced by cellulose and xylan. No difference was found in the high (sn26-1) and low (9074) aggressive isolates. Addition of cell wall-degrading enzyme mixtures, not high xylanase alone, to a spore suspension of a low aggressive isolate (9074) caused a limited increase in tissue necrosis. We conclude that the cell wall degrading enzymes play a role in early penetration of the host by the fungus, but they are not important elicitors for disease development.
    Źródło:
    Plant Breeding and Seed Science; 2005, 51; 21-30
    1429-3862
    2083-599X
    Pojawia się w:
    Plant Breeding and Seed Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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