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    Wyświetlanie 1-11 z 11
    Tytuł:
    Novel designs and technologies for cell engineering
    Autorzy:
    Ziółkowska, K.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115526.pdf
    Data publikacji:
    2011
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    lab-on-a-chip
    multicellular tumor spheroid
    cancer
    in vitro cell culture
    cell engineering
    Opis:
    Microfluidic devices, such as lab-on-a-chip systems, are highly advantageous for cell engineering and cell based assays. It is a particularly useful approach for development of the in vitro cellular systems mimicking the in vivo environment. In this paper, a novel lab-on-a-chip device for three-dimensional human cell culture and anticancer drug testing is presented. Cells were cultured as Multicellular Tumor Spheroids (MCTS) — the best cancer tumor model developed so far. Diff erent designs were tested and novel technique of microfabrication in poly(dimethylsiloxane) was developed. MCTS were cultured in a system of polymeric microwells, with the network of microfluidic channels for culture medium flow. Design included optimal shear stress and proper nutrients supply for cultured cells. Final design provided MCTS culture for four weeks with the homeostasis-like state achievement, which is characteristic for the in vivo situation.
    Źródło:
    Challenges of Modern Technology; 2011, 2, 4; 54-60
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    ‘Lab-on-a-chip’ for cell engineering: towards cellular models mimicking in vivo
    Autorzy:
    Ziółkowska, K.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115885.pdf
    Data publikacji:
    2011
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    Lab-on-a-chip
    microfluidic tools
    cancer
    cell culture
    Multicellular Tumor Spheroid (MCTS)
    Opis:
    One of the main scopes of modern cell engineering is development of cellular models that can replace animals in drug screening and toxicological tests, so called alternative methods. Construction of the alternative model is a very challenging task due to a richness of factors creating the in vivo environment. The monolayer cell culture — cultivation of adhesive cells on artificial surfaces such as glass or polymer — lack most of the in vivo-like interactions, but still is the only tool for the majority of applications. One of the most prospective approaches on mimicking in vivo environment is “Lab-on-a-chip” technology. Microfluidic devices offer lots of advantages over traditional in vitro culture, e.g. much higher cell volume-to-extracellular fluid volume ratio or possibility of regulation of hydrodynamic stress. This presentation aims to introduce latest advances of our team in microfluidic cell culture devices. Our novel approach is to cultivate three dimensional multicellular aggregates (spheroids) in microenvironments arranged in a microfluidic system. The geometry and materials of the system allow for cultivation, observation and analysis of multicellular spheroids. The results presented concern multicellular tumor spheroids (MCTS) rising from human cancer cells, which are considered to represent most of the conditionings of cancer tumor in vivo. The fully developed MCTS microdevice will be a reliable tool for anticancer drug screening, as the results most likely will be in a close accordance with the results obtained in vivo.
    Źródło:
    Challenges of Modern Technology; 2011, 2, 1; 79-82
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Miniaturized device for a cell lysis process
    Autorzy:
    Kwapiszewski, R.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115684.pdf
    Data publikacji:
    2011
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    microdevice
    microfludic chip
    chemical cell lysis
    sheath flow
    hydrodynamic focusing
    Opis:
    Single-cell studies are crucial for gaining knowledge on complexity of intracellular processes. In many cases, carrying researches into cell ingredients must be proceeded by a lysis process. Cell lysis leads to disintegration of the plasma membrane which is the barrier separating cell contents from the environment. However, investigations at the cellular level would not be possible without proper miniaturized tools, which offer many advantages as low reagents consumption, short reaction time, integration, automation or versatility. The goal of this work was to design and develop a microfluidic chip for a chemical cell lysis process. The geometry of a microsystem presented is based on the hydrodynamic focusing of a cell suspension stream. Applying non-denaturing cell lysis buffer enables to analyze released cell ingredients during next steps of investigations.
    Źródło:
    Challenges of Modern Technology; 2011, 2, 2; 50-52
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of a Point-of-Care system for early diagnostics of genetic diseases
    Autorzy:
    Kwapiszewski, R.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115790.pdf
    Data publikacji:
    2012
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    lysosomal storage disorders
    Fabry disease
    Gaucher disease
    early diagnostics
    intracellular enzyme activity
    Point-of-Care system
    lab-on-a-chip system
    Opis:
    Lysosomal storage disorders (LSDs) represent a group of more than 45 genetically inherited diseases caused by the absence or deficiency of one or more specific lysosomal enzymes. Nowadays, there is a lack of reports on fast, reliable methods for the diagnostics of LSDs. Currently applied diagnostic approaches generate many false-negative and false-positive results, which results in classification of patients to inappropriate therapeutic groups. Moreover, these methods are time-consuming (even 20 hours), and are carried out only in a few laboratories in the world. The goal of this work was to develop a method and a tool, a Point-of-Care system, for diagnostics of LSDs. The polymeric microdevice consists of a cell lysis module, a mixing microchannel and an optical detection module. The system enables to determine the activity of α-galactosidase (deficient in Fabry disease), and to reduce the time of analysis to 10 min. Due to its easy fabrication steps and low price, it seems to be a prospective tool for a point-of-care approach.
    Źródło:
    Challenges of Modern Technology; 2012, 3, 2; 6-8
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microfluidic devices — application in anticancer studies
    Autorzy:
    Jędrych, E.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115929.pdf
    Data publikacji:
    2012
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    microfluidic system
    PDMS
    adherent cell culture
    concentration gradient generator (CGG)
    cytotoxicity tests
    photodynamic therapy (PDT) procedures
    Opis:
    A rapidly growing pharmaceutical industry requires faster and more efficient ways to find and test new drugs. One of the new method for cell culture and examining the toxic effects of drugs is application of microfluidic systems. They provide new types of microenvironments and new methods for investigation of anticancer therapy. The use of microsystems is a solution that gives the opportunity to reduce not only cost and time, but also a number of tests on animals. In this paper we present designed and fabricated hybrid microfluidic systems which are applicable for cell culture, cell based cytotoxicity assays and photodynamic therapy procedures. Polydimethylsiloxane (PDMS) and sodium glass were used for fabrication of microdevices. The designed geometry of the microdevices includes cell culture microchambers and a concentration gradient generator (CGG). The CGG enables to obtain diff erent concentrations of tested drugs in a single step, which is a significant simplification of cytotoxicity assay procedure. In the designed microsystems three various cell lines (normal and carcinoma) were cultured and analyzed.
    Źródło:
    Challenges of Modern Technology; 2012, 3, 2; 3-5
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Lab-on-a-chip microdevice with contactless conductivity detector
    Autorzy:
    Blaszczyk, K.
    Chudy, M.
    Brzozka, Z.
    Dybko, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/221617.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    lab-on-a-chip
    poly(dimethylsiloxane)
    contactless conductivity
    pseudoelectrodes
    microfluidic
    Opis:
    This paper describes a new contactless conductivity detector, whose electrodes are constructed of microchannels filled with solution of KCl - called pseudoelectrodes. The lab-on-a-chip microdevice was fabricated in poly(dimethylsiloxane) PDMS, using a moulding technique. The mould was made from a dry negative photoresist with a thickness of 50 µm. During the tests, the dimension and arrangement of pseudoelectrodes' microchannels were evaluated. The analyte was pumped into the microchannel using a syringe pump with a flow rate of 50 µL/min. Reproducible changes of the signal were obtained.
    Źródło:
    Metrology and Measurement Systems; 2013, 20, 2; 299-306
    0860-8229
    Pojawia się w:
    Metrology and Measurement Systems
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    An integrated gas removing system for microfluidic application. Design and evaluation
    Autorzy:
    Hofman, I. M.
    Ziółkowska, K.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115931.pdf
    Data publikacji:
    2013
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    gas removing system
    debubbler
    debubbling system
    lab-on-a-chip
    microfluidics
    long-term cell culture
    cell spheroids
    HT-29 cells
    Opis:
    Microfluidic systems are used in a wide range of applications, including medical diagnostics, cell engineering and bioanalytics. In this work we focused on “Lab-on-a-chip” microsystems for cell cultivation. A troublesome problem of gas bubbles entering microdevices causing signal interferences and cells damage was emphasized. A novel, integrated debubbler in the form of cylindrical traps covered with thin PDMS membrane was designed and manufactured. Demonstrated debubbler was successfully applied in a long-lasting culture of HT-29 cell aggregates.
    Źródło:
    Challenges of Modern Technology; 2013, 4, 3; 10-15
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    New microfluidic device for lactate dehydrogenase (LDH) activity analysis
    Autorzy:
    Jędrych, E.
    Mazur, M.
    Grabowska-Jadach, I.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115778.pdf
    Data publikacji:
    2012
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    lactate dehydrogenase (LDH) activity
    microfluidic system
    PDMS
    adherent cell culture
    cytotoxicity analysis
    Opis:
    In this paper, we present cytotoxicity analysis (determination of lactate dehydrogenase — LDH activity performed in a designed and fabricated microfl uidic system. This method allowed for analysis of a supernatant collected from A549 (human lung cancer) and HT-29 (human colon cancer epithelial) cells, which were incubated for 24 h with selected compounds. LDH is an intracellular enzyme present in tissues, which is released into the supernatant caused by membrane damage or cell lyses. In our tests, LDH-Cytotoxicity Assay Kit (BioVision) was used. The toxic eff ect of drugs was measured in the developed microsystem made of PDMS (poly(dimethylsiloxane)). Analytical reaction took place in the special designed microchannel geometry. Then, the LDH activity was measured at 490 nm using spectrophotometer. In subsequent experiments, appropriate conditions for measurements using a microfl uidic system were chosen. It was found that the selected reagent is sensitive to temperature changes and light exposure. Reaction time in the microsystem was determined by changes of fl ow rates of reagents. Afterwards, for the various reaction time, the toxic eff ect of 5-fl uorouracil, celecoxib and 1,4-dioxane was performed. The obtained results were compared with the results carried out in 96-well plates. Based on these results, it was noted that the enzymatic reaction time in the microsystem is shorter than in 96-well plate. Moreover, the advantage of using microsystem is also the small amount of reagents.
    Źródło:
    Challenges of Modern Technology; 2012, 3, 4; 3-8
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Inżynieria komórkowa w systemach lab-on-a-chip
    Cell engineering in lab-on-chip systems
    Autorzy:
    Tomecka, E.
    Tokarska, K.
    Jastrzębska, E.
    Chudy, M.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/172147.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    inżynieria komórkowa
    mikrosystemy przepływowe
    dwuwymiarowa hodowla komórkowa
    trójwymiarowa hodowla komórkowa
    cell engineering
    microfluidic systems
    two-dimensional cell culture
    three-dimensional cell culture
    Opis:
    Lab-on-a-chip systems are promising tools in the field of cell engineering. Microfluidic systems are integrated microlaboratories consisting of many microstructures such as microchannels and microchambers, which can be used for cell analysis and cell culture. Appropriately designed geometry of the chip allows to mimic in vivo conditions. Microsystems enables continuous culture medium perfusion. During cell culture, regulation of the flow rate of medium is possible, which allows to control conditions of the cultivation. In this paper we present a review of microfluidics systems which are used in cell engineering. We describe methods of microsystems fabrication, parameters which influence cell proliferation in microscale and examples of microsystems for cell analysis and cell culturing. Microfluidic systems for maintaining cell culture are mainly fabricated of poly(dimethylsiloxane) (PDMS) and glass, non-toxic materials for cells. The most commonly used method for fabrication of PDMS microsystems is photolithography and replica molding techniques. Cell culture in microsystems can be carried out in two ways: as a two-dimensional (2D) cell culture and three-dimensional (3D) cell culture. In two-dimensional culture cells grow as a monolayer on a flat surface of microchambers or microchannels. Microsystems for two-dimensional cell culture are widely described in the literature. They are mainly used for: (i) cell proliferation after exposure to external stimuli, (ii) testing the activity of cytotoxic drugs, (iii) interactions and cell migration and (iv) the evaluation of procedures applicable in tumor therapy e. g. photodynamic therapy. However, two-dimensional cell culture do not mimic fully in vivo conditions. In living organisms cells grow spatially creating three-dimensional structures like tissues. Therefore, nowadays microsystems for 3D cell culture are being developed intensively. Three-dimensional cell culture in microfluidic systems can be achieved in three ways: by the design of suitable geometry and topography of microchannels, by the use of hydrogels or by spheroids formation. Three-dimensional cell culture in microfluidic systems are much better experimental in vitro models than cell culture in traditional culture vessels. It is the main reason why microsystems should be still improved, as to become widely used research tools in cellular engineering.
    Źródło:
    Wiadomości Chemiczne; 2015, 69, 9-10; 909-929
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Photodynamic therapy procedures in the microfluidic system
    Autorzy:
    Jędrych, E.
    Pawlicka, Z.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115635.pdf
    Data publikacji:
    2010
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    photodynamic therapy (PDT)
    microfl uidic culture system
    PDMS
    adherent cell culture
    concentration gradient generator (CGG)
    5-aminolevulinic acid (ALA)
    Opis:
    Evaluation of the effi ciency of photodynamic therapy (PDT) in a hybrid microfl uidic culture system was studied. The geometry of the utilized microsystem for PDT procedures consists microchambers for cell culture and microchannels, which create a concentration gradient generator (CGG). 5-aminolevulinic acid (ALA) as a precursor of the photosensitizer was used. The geometry of the microchip allowed to test diff erent concentrations of ALA in a single assay. Evaluation of the effi ciency of photodynamic therapy was determined 24 hours after PDT procedure (irradiation with light which induced accumulated in carcinoma cells). The performed microsystem contained two independent micropatterns, that enables examination simultaneously various cell lines (carcinoma and normal) or various photosensitizers.
    Źródło:
    Challenges of Modern Technology; 2010, 1, 1; 30-33
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Potentiometric detection of the metabolic activity of human tumor cells
    Autorzy:
    Kwapiszewska, K.
    Stępień, K.
    Sendys, P.
    Chudy, M.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115519.pdf
    Data publikacji:
    2013
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    cancer
    in vitro cell culture
    potentiometry
    cell culture monitoring
    Opis:
    Monitoring of cellular viability is a key part of toxicological assays in vitro. On-line monitoring of metabolic activity would be particularly useful for evaluation of responses to potential therapeutic compounds. Current assays are mostly based on fluorescent dyes and optical detection methods. These methods offer high sensitivity and specificity, however are not suitable for long-term on-line observations. Electrochemical methods can be an alternative for current protocols. Electrochemical detection is low cost and label-free, therefore suitable for long-term cell culture monitoring. In this work investigations on human cancer cells viability will be presented. Cells were cultured as two-dimensional monolayer or three-dimensional spheroids. Different cell culture media were examined. Potentiometric detection was used for continuous monitoring of cell culture as well as end-point investigations. Different growth phases were identified using applied method. Finally, response to an anticancer drug was successfully observed.
    Źródło:
    Challenges of Modern Technology; 2013, 4, 1; 41-46
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-11 z 11

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