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Wyszukujesz frazę "Ścisłowska-Czarnecka, A." wg kryterium: Autor

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    Wyświetlanie 1-13 z 13
    Tytuł:
    Immobilization of collagen - an effective method of improving cell adhesion on polymeric materials
    Autorzy:
    Pamuła, E.
    Ścisłowska-Czarnecka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/284946.pdf
    Data publikacji:
    2007
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    polimery
    polymers
    Opis:
    Surface properties of poly(L-lactide-co-glycolide) (PLG), and two reference materials: hydrophobic polystyrene (PS) and hydrophilic tissue culture polystyrene (TCPS) were modified by collagen adsorption. The morphology of the obtained collagen film was observed by using atomic force microscopy. On PLG and TCPS collagen layer was uniform, while on PS collagen formed isolated patches. The differences in supramolecular organization of collagen were due to differences in surface wettability. The behaviour of L929 fibroblasts incubated on all raw and collagen-modified surfaces was then evaluated. The best adhesion and spreading of cells, as expected, were observed on TCPS. Collagen adsorbed on PLG and PS considerably improved adhesion and spreading of fibroblasts.
    Źródło:
    Engineering of Biomaterials; 2007, 10, no. 63-64; 20-23
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fibroblast biological activity on poly(l-lactide) and poly(l-lactide-co-trimethylene carbonate)
    Autorzy:
    Ścisłowska-Czarnecka, A.
    Pamuła, E.
    Kołaczkowska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/284073.pdf
    Data publikacji:
    2011
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    fibroblasts
    biomaterials
    PLLA
    Opis:
    Poly-L-lactide (PLLA) is acknowledged biocompatible polyester. However, it possesses high crystallinity/brittleness/stiffness and requires long time for complete degradation. In the current study we present data on PLTMC, a copolymer of L-lactide and trimethylene carbonate (TMC). Poly(trimethylene carbonate) (PTMC) is characterised by good mechanical properties and rapid degradation rate and for this it might possess new desired features for medical applications. During the experiments, adhesion and activity of fibroblasts cultured on PLLA and PLTMC were studied and compared during two time points of 3 and 5 days. On day 3, the number of adherent fibroblasts was compromised when fibroblasts were cultured in the presence of PLTMC but the proper adherence was recovered by day 5. The same pat-tern was observed when we evaluated some activity parameters of fibroblasts. In particular, the release of proteins and nitric oxide was studied as the increased levels of the mediators might indicate unwanted inflammatory-like condition. Overall, the results suggest that the synthesized PLTMC initially shows unwanted effects on fibroblasts but with the time these effects are abolished. Therefore PLTMC seems to represent a new material that is non-cytotoxic and compatible with the living cells.
    Źródło:
    Engineering of Biomaterials; 2011, 14, 102; 7-10
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ biomateriałów na przyleganie i aktywność mysich fibroblastów L929
    Effects of biomaterials on adhesion and activity of murine fibroblasts L929
    Autorzy:
    Ścisłowska-Czarnecka, A.
    Pamuła, E.
    Płytycz, B.
    Kołaczkowska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/285238.pdf
    Data publikacji:
    2008
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    biomateriały
    fibroblasty
    przeszczepy chirurgiczne
    biomaterials
    fibroblasts
    transplantation
    Źródło:
    Engineering of Biomaterials; 2008, 11, no. 81-84; 83-86
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Enhanced cell integration to titanium-grafit composite
    Autorzy:
    Menaszek, E.
    Ścisłowska-Czarnecka, A.
    Deptuła, P.
    Dąbrowski, J. R.
    Powiązania:
    https://bibliotekanauki.pl/articles/285876.pdf
    Data publikacji:
    2010
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    composites
    titanium alloys
    implants
    Źródło:
    Engineering of Biomaterials; 2010, 13, no. 99-101; 122
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Chemical modification of poly ε-caprolactone with wollastonite and its influence on biological properties of osteoblast like-cells MG-63
    Autorzy:
    Ścisłowska-Czarnecka, A.
    Menaszek, E.
    Kołaczkowska, E.
    Błażewicz, M.
    Podporska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/284898.pdf
    Data publikacji:
    2011
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    polycaprolactone
    wollastonite
    osteoblast-like MG-63 cells
    cell adhesion
    viability
    proliferation
    alkaline phosphatase
    Opis:
    PCL (poly-ε-caprolactone) is a biocompatible and biodegradable polymer of aliphatic polyester group. However, PCL does not effectively bind to the bone in contrast to bioactive inorganic compounds such as wollastonite. For this wollastonite (WS) is regarded as a potential bioactive material for bone tissue engeenering although its main drawback is brittlennes. Therefore we synthesized polymer nanocomposite materials composed of poly-ε-caprolactone and wollastonite (PCL/wollastonite) containing either 0.5% or 5% of the latter modifying filler. And we aimed to verify biological properties of the nanocomposite PCL/WS materials, in comparison to the pure PCL, on cultures of osteoblast-like cells MG-63. The study revealed that the adherence of the osteoblast-like cells to the tested materials was enhanced by the PCL modification (PCL/5WS > PCL/0.5WS > PCL) while cell viability/proliferation was not altered. Furthermore, the activity of alkaline phosphatase indicative of osteoblast differentiation (maturation) was enhanced when the cells were cultured with either PCL/5WS or PCL/0.5WS. Overall, our results indicate that PCL-modified wollastonite improves biological properties of the basic biomaterial suggesting its potential usefulness/application for the bone tissue regeneration.
    Źródło:
    Engineering of Biomaterials; 2011, 14, 102; 11-14
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of titanium alloy modified with a-C:N:H and a-SiCxNY(H) coatings on adhesion and immune response of human osteoblast-like MG-63 cells
    Autorzy:
    Ścisłowska-Czarnecka, A.
    Menaszek, E.
    Kołaczkowska, E.
    Januś, M.
    Stypuła, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/284995.pdf
    Data publikacji:
    2008
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    titanium alloy
    a-C:N:H and a-SiCxNy(H) coatings
    osteoblast-like MG-63 cells
    cell adhesion
    cytokines
    Opis:
    The study was conducted in order to determine the effects of modified titanium alloy (Ti-6Al-4V) surfaces on the biological response of a human osteoblast-like cell line. MG-63 cells were cultured on disk-shaped Ti-alloys: unmodified, and covered with a-C:N:H or a-SiCxNy(H) layers. Interactions between materials and cells were examined through determination of cells adhesion and secretion of cytokines involved in the development of immune response.
    Źródło:
    Engineering of Biomaterials; 2008, 11, no. 81-84; 126-128
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Porous titanium scaffolds with modified surface: in vitro cell biology assessment
    Autorzy:
    Ścisłowska-Czarnecka, A.
    Menaszek, E.
    Szaraniec, B.
    Chłopek, J.
    Kołaczkowska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/285246.pdf
    Data publikacji:
    2009
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    osteoblasts
    cytokines
    cell adherence
    morphology
    titanium
    hydroxyapatite
    bioglass
    CaO-SiO2
    Opis:
    Interaction of host cells with a biomaterial surface is important for biocompatibility and thus is essential for biomedical applications. Therefore investigations are undertaken to scrutinize for an appropriate surface coating with physical and chemical properties minimizing undesirable activation of immunological response. For this the current study was aimed at examining the effects of different surface modifications of titanium by its coating with ceramic materials - hydroxyapatite, bioglass and CaO-SiO2on osteoblast morphology and secretory activity. Titanium is known for its excellent mechanical properties but its surface has low bioactivity. We report that CaO-SiO2coating decreased a number of attached osteoblasts and altered their morphology. Moreover, the ceramic coatings temporarily upregulated release of pro-inflammatory cytokines IL-6 (all of them) and TNF-α (CaO-SiO2). However, overall the levels of the cytokines were low. In contrast, levels of neutrophil-attracting chemokine IL-8 were the highest. IL-8 was produced mostly by cells incubated with hydroxyapatite titanium coating in contrary to those incubated with either bioglass or CaO-SiO2titanium modifications. In conclusion, the titanium coated with ceramics such as hydroxyapatite or bioglass had the best effect on cell adhesion; however, hydroxyapatite might potentially stimulate destructive neutrophils while CaO-SiO2-coating has a negative effect on cell adhesion.
    Źródło:
    Engineering of Biomaterials; 2009, 12, 88; 5-10
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of cytocompatibility of PLGA and PGLA-based nanocomposite biomaterials in osteoblast cultures
    Autorzy:
    Ścisłowska-Czarnecka, A.
    Stodolak-Zych, E.
    Prawdzik, E.
    Boguń, M.
    Menaszek, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/285320.pdf
    Data publikacji:
    2012
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    kompozyty
    nanokompozyty
    cells materials interaction
    cells factors
    composites
    nanocomposites
    Opis:
    The aim of this study was to evaluate biocompatibility of multilevel composites based on bioresorbable poly(lactide-co-glycolide) (PGLA). Polymer matrix was modified with multidimensional (MD) short biopolymer fibers of calcium alginate (CA) containing bioactive ceramic nanoparticles (nanohydroxyapatite - HA or nanosilica - SiO2). The nanocomposite fibres present in the polymer samples influenced cells morphology, viability and secretory activity which was estimated using human osteoblasts cells (NHOst). The results indicate that biodegradable nanocomposite CA-HA/PGLA improves biological properties of the basic biomaterial (PGLA) suggesting its potential application for bone tissue engineering.
    Źródło:
    Engineering of Biomaterials; 2012, 15, 112; 2-5
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biocomatibility study of BOC polymer mesh enriched with HAp and TCP covered by PCL fibres
    Autorzy:
    Menaszek, E.
    Ścisłowska-Czarnecka, A.
    Draczyński, Z.
    Bogun, M.
    Stodolak-Zych, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/286223.pdf
    Data publikacji:
    2012
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    polymer mesh
    HAp
    TCP
    osteoblasts NHOst
    Opis:
    The study was conducted in order to determine the biocomatibility of polimer mesh based on BOC and enriched with HAp or TCP coverd by PCL sub-micrometric fibres. Human osteoblast cell line NHOst was cultured in standard conditions on disk-shaped polymer samples. Interactions between materials and cells were examined through microscopic observation of cells' adhesion and morphology, and tests of viability/proliferation and cytotoxicity. The study proved the biocompatibility of all examined materials, though the surface of TCP enriched polymer didn't promote the adhesion of cells.
    Źródło:
    Engineering of Biomaterials; 2012, 15, no. 116-117 spec. iss.; 151-152
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biocompatibility of hybrid fibrous materials basing on poly-L/DL-lactide
    Autorzy:
    Stodolak, E.
    Ścisłowska-Czarnecka, A.
    Błażewicz, M.
    Bogun, M.
    Mikolajczyk, T.
    Menaszek, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/286193.pdf
    Data publikacji:
    2010
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    hybrid biomaterials
    composites
    nanocomposite materials
    Opis:
    Hybrid biomaterials due to their unique structure may become an alternative for many popular composite and nanocomposite materials. Multilevel modification of their matrix manifesting itself in the presence of particles of different sizes i.e., micrometric, submicrometric and nanometric together with the variety of shapes of a modyfing phase (nanometric fibres, submicron particles, coated nanoparticles) and its different chemical character make the hybrid materials similar to natural tissue. Bone tissue structure is particulary close to this model in which collagen fibres and hydroxyapatite particles and nanoparticles have not only different form but first of all they play different role in the tissue which depends on their chemical nature. In the biomedical engineering syntetic hybride biomaterials are usually produced using resorbable and degradable polymer matrices and inorganic filers (ceramic bioactive particles; HAp, TCP, SiO2) or organic filers (collagen, polysaccharides e.g. alginate fibres). The main function of the modyfing phase is inprovement of the polymer matrix leading to bioactive, stronger material showing high biofunctionality. Production of hybrid materials is based mainly on experimental works, which is related to the presence in their matrix few phases with different properties which may interact. Hybrid materials do not follow the rule of mixtures thus it is difficult to predict behaviour of a material in which co-exis different chemical and phisical phases. In the work hybrid composite foils were produced in which modyfing phase consisted in; nanocomposite calcium alginate fibres modyfied with ceramic nanoparticles; HAp (CAH fibres), TCP (CAT fibres), SiO2 (CAS fibres) and MMT (CAM fibres). Short fibres were subjected to additional size reduction in vibration ball mill resultiong in submicron and nanometric phases. Size of the particels after grinding was determined by screening analysis and DLS method (for particels smaller than 500 nm). It was observed than the population of short fibres consist in three fractions i.e.; micrometric (~2μm, 50 wt.%), submicrometric (500–800 nm, 40 wt.%) and nanometric ( below 500 nm, 10 wt.%). The fibres and products of their grinding were homogenised in P(L/ DL)LA polymer solution (poly-L/DL-lactide, Purarorb 80, Purac Germany). A hybride material in the form of thin foils containing 2 wt.% of a modyfing phase were subjected to durability tests consisting in incubation in distilled water (30 days/37C). Monitoring of the medium pH and conductivity did not show changes related to harmful products of their decomposition. Osteoblast-like cells from MG-63 line contacted with the surface of the materials showed high viability (MMT test) comparable with the reference material (TCPS). High degree of adherence of the cells to the materal surface (CV test) testifies of potential abilities of the material stimulating proliferation of bone tissue cells. The highes rate of dynamic growth (increase of the cells number after 7 days of incubation) was observed for the material which was modified with CAS fibres and products of their grinding. The performed investigations have a preliminary character. Their results testify for potential osteoconductive or osteoinductive abilities of hybride materials basing on P(L/DL)LA and alginate nanocomposite fibres.
    Źródło:
    Engineering of Biomaterials; 2010, 13, no. 99-101; 110
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fibrous structures based of natural polymers for tissue engineering applications
    Autorzy:
    Stodolak-Zych, E.
    Ścisłowska-Czarnecka, A.
    Kolesinska, B.
    Cieślak, M.
    Puchowicz, D.
    Kaminska, I.
    Bogun, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/285455.pdf
    Data publikacji:
    2018
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    polymers
    tissue engineering
    spectroscopy
    Źródło:
    Engineering of Biomaterials; 2018, 21, 148; 63
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative study of hydroxyapatite and hydroxyapatite mixed with bioglass coatings of metallic implants, deposited by PLD method
    Autorzy:
    Mróz, W.
    Jedyński, M.
    Burdyńska, S.
    Prokopiuk, A.
    Ślósarczyk, A.
    Menaszek, E.
    Ścisłowska-Czarnecka, A.
    Łączka, M.
    Cholewa-Kowalska, K.
    Niedzielska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/286137.pdf
    Data publikacji:
    2008
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    implants
    hydroxyapatite
    bioglasses
    Opis:
    Hydroxyapatite and bioglasses are important bioactive materials as they exhibit direct bonding with human bone. Therefore they are used as coatings for metallic implants. The aim of the present study was to compare two types of layers: monophase hydroxyapatite (HA) and composite-type HA+BG hydroxyapatite mixed with bioglass (BG) (BG 50% of weight) during the initial stage of their interaction with cell medium. In vitro studies were performed in order to determine the effect of the investigated layers on cell response. After 3 and 7 days the behaviour of the cells grown on the above surfaces was estimated through determination of the cell adhesion (CV colorimetric assay). Cell morphology and properties of biomaterials surfaces were analysed by atomic force microscopy (AFM).
    Źródło:
    Engineering of Biomaterials; 2008, 11, no. 81-84; 121-123
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The membrane with polylactide and hyaluronic fibers for skin substitute
    Autorzy:
    Stodolak-Zych, E.
    Rozmus, K.
    Dzierzkowska, E.
    Zych, Ł.
    Kapacz-Kmita, A.
    Gargas, M.
    Kołaczkowska, E.
    Cieniawska, M.
    Książek, A.
    Ścisłowska-Czarnecka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/306599.pdf
    Data publikacji:
    2018
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    nanowłókna
    elektroprzędzenie
    kwas hialuronowy
    polilaktyd
    nanofibrous scaffold
    electrospinning
    skin substitute
    hyaluronic acid
    polylactide
    Opis:
    Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells. We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation. Methods: Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions. By changing the parameters, it was possible to control the diameter and properties of both polymer fibers. The spinning solution were characterized by surface tension and rheology. A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA. Structure of the PLA/HA nonwoven was analyzed using spectroscopy (FTIR/ATR). Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions. Results: The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt. solution of the polymer, dissolved in a 3:1 mixture of DCM:DMF at 45 °C. The hyaluronic fibers were prepared from a 12% wt. solution of the polymer dissolved in a 2:1 mixture of ammonia water and ethyl alcohol. All materials were biocompatible but to a different degree. Conclusions: The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers. The material positively underwent biocompatibility testing in contact with fibroblasts.
    Źródło:
    Acta of Bioengineering and Biomechanics; 2018, 20, 4; 91-99
    1509-409X
    2450-6303
    Pojawia się w:
    Acta of Bioengineering and Biomechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-13 z 13

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