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Tytuł:
A new approach to phosphorylation of nucleosides using oxyonium phosphobetaines as intermediates
Autorzy:
Materna, Magdalena
Stawinski, Jacek
Sobkowski, Michał
Powiązania:
https://bibliotekanauki.pl/articles/16673995.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
synthesis
phosphorylation
N-oxides
nucleotides
oxyonium phosphobetaines
H-phosphonates
Opis:
Oxyonium phosphobetaines are recently discovered molecules with a unique ¯O–P–O–N+ bond system, which makes them useful and versatile intermediates for the synthesis of phosphates and their derivatives. In this paper, the preliminary data on the application of these compounds in nucleoside phosphorylation were presented.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 1; 93-99
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
NH4plus -mediated protein phosphorylation in rice roots
Autorzy:
Zhu, X.F.
Cai, W.H.
Jung, J.H.
Xuan, Y.H.
Powiązania:
https://bibliotekanauki.pl/articles/19339.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
ammonium
protein phosphorylation
rice
root
phosphoproteomics
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 2
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phosphorylation of basic amino acid residues in proteins: important but easily missed
Autorzy:
Cieśla, Joanna
Frączyk, Tomasz
Rode, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1039902.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
basic amino acids
posttranslational modification
phosphorylation
acid-labile
base-stable
phosphoramidate
Opis:
Reversible phosphorylation is the most widespread posttranslational protein modification, playing regulatory role in almost every aspect of cell life. The majority of protein phosphorylation research has been focused on serine, threonine and tyrosine that form acid-stable phosphomonoesters. However, protein histidine, arginine and lysine residues also may undergo phosphorylation to yield acid-labile phosphoramidates, most often remaining undetected in conventional studies of protein phosphorylation. It has become increasingly evident that acid-labile protein phosphorylations play important roles in signal transduction and other regulatory processes. Beside acting as high-energy intermediates in the transfer of the phosphoryl group from donor to acceptor molecules, phosphohistidines have been found so far in histone H4, heterotrimeric G proteins, ion channel KCa3.1, annexin 1, P-selectin and myelin basic protein, as well as in recombinant thymidylate synthase expressed in bacterial cells. Phosphoarginines occur in histone H3, myelin basic protein and capsidic protein VP12 of granulosis virus, whereas phospholysine in histone H1. This overview of the current knowledge on phosphorylation of protein basic amino-acid residues takes into consideration its proved or possible roles in cell functioning. Specific requirements of studies on acid-labile protein phosphorylation are also indicated.
Źródło:
Acta Biochimica Polonica; 2011, 58, 2; 137-148
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of vanadate on Pichia pastoris growth, protein kinase A activity and ribosomal protein phosphorylation.
Autorzy:
Frajnt, Magdalena
Cytryńska, Małgorzata
Jakubowicz, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/1043701.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
protein phosphorylation
vanadate
Pichia pastoris
PKA
ribosomal proteins
protein kinases
Opis:
It was found that wild type yeast Pichia pastoris can tolerate vanadate concentration as high as 25 mM in the growth medium. Moreover, four vanadate-resistant P. pastoris strains designated JC100/1, JC100/3, JC100/9 and JC100/15 exhibiting tolerance up to 150 mM vanadate were selected. Growth of P. pastoris was correlated with vanadate to vanadyl reduction and its accumulation in the growth medium. In two selected strains, JC100/9 and JC100/15, protein kinase A activity was much higher in comparison to the wild type strain even without vanadate addition to the growth medium. Moreover, in the presence of vanadate, protein kinase A activity was significantly increased in the wild type and the vanadate-resistant JC100/1 and JC100/3 strains. It was also found that phosphorylation of a 40 kDa protein associated with ribosomes occured in all vanadate-resistant strains from the logarithmic, while in the wild type strain from the stationary growth phase. From the presented results it can be concluded that a protein kinase A signalling pathway(s) might be involved in the mechanism of P. pastoris vanadate resistance. The results also indicate a possible role of the 40 kDa protein in protection of P. pastoris against vanadate toxicity.
Źródło:
Acta Biochimica Polonica; 2002, 49, 4; 959-968
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tissue variation of mitochondrial oxidative phosphorylation efficiency in cold-acclimated ducklings
Autorzy:
Salin, Karine
Teulier, Loïc
Rey, Benjamin
Rouanet, Jean-Louis
Voituron, Yann
Duchamp, Claude
Roussel, Damien
Powiązania:
https://bibliotekanauki.pl/articles/1040299.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
liver
skeletal muscle
thermogenesis
mitochondrial oxidative phosphorylation
proton conductance
Opis:
We investigated the oxidative phosphorylation efficiency of liver and gastrocnemius muscle mitochondria in thermoneutral and cold-acclimated ducklings. The yield of oxidative phosphorylation was lower in muscle than in liver mitochondria, a difference that was associated with a higher proton conductance in muscle mitochondria. Cold exposure did not affect oxidative phosphorylation efficiency or basal proton leak in mitochondria. We conclude that the basal proton conductance of mitochondria may regulate mitochondrial oxidative phosphorylation efficiency, but is not an important contributor to thermogenic processes in cold-acclimated ducklings.
Źródło:
Acta Biochimica Polonica; 2010, 57, 4; 409-412
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phosphorylation sites of HER2/c-erbB-2: role in cell growth and in disease
Autorzy:
Khurshid, Rukhshan
Saleem, Mahjabeen
Gul-e-Raana, -
Akhthar, Muhammad
Powiązania:
https://bibliotekanauki.pl/articles/1039198.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phosphorylation sites
HER2/c-erbB-2
cell growth and disease
Opis:
The protein kinase c-erbB-2 belongs to the family of receptor tyrosine kinase and is involved in oncogenesis. The present study predicts different phosphorylation sites of HER2/c-erbB-2 which are important in preventing or developing cancer, especially breast cancer. Sequence homology showed highest homology (77%) with epidermal growth factor receptor kinase domain. According to PROSITE search result, active sites of c-erbB-2 are N-lobe (glycine rich phosphate binding loop). Catalytic loop with presumptive catalytically active of Asp108 is phosphorylated by tyrosine protein kinase. A-loop, activation loop, becomes phosphorylated and activates the substrate binding. The study strengthens our knowledge regarding HER2 signaling by the detection of uncharacterized signaling proteins, establishing phosphorylation of an activation loop and helps us to make assumptions about the role of such previously unidentified proteins. On the basis of importance of HER2 in breast cancer as well as in other diseases, this study provides fruitful information for designing new therapeutic strategies.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 699-703
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Komputerowe modelowanie regulacji fosforylacji oksydacyjnej
Computer modelling of regulation of oxidative phosphorylation
Autorzy:
Korzeniewski, Bernard
Powiązania:
https://bibliotekanauki.pl/articles/1198616.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Opis:
Quantitative description of complex biological systems is presented using as an example a dynamic computer model of oxidative phosphorylation in mitochondria. Theoretical studies concerning the regulation of this process in response to varying energy demand in intact skeletal muscles and heart are discussed. Computer simulations demonstrate that it is possible to predict theoretically, at least to try to, the existence of completely new phenomena in biological systems.
Źródło:
Kosmos; 2005, 54, 2-3; 163-173
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Is MLC phosphorylation essential for the recovery from ROCK inhibition in glioma C6 cells?
Autorzy:
Korczyński, Jarosław
Sobierajska, Katarzyna
Krzemiński, Patryk
Wasik, Anna
Wypych, Dorota
Pomorski, Paweł
Kłopocka, Wanda
Powiązania:
https://bibliotekanauki.pl/articles/1039964.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
RhoA
myosin II
calcium signaling
actin
MLC phosphorylation
Opis:
Inhibition of Rho-associated protein kinase (ROCK) activity in glioma C6 cells induces changes in actin cytoskeleton organization and cell morphology similar to those observed in other types of cells with inhibited RhoA/ROCK signaling pathway. We show that phosphorylation of myosin light chains (MLC) induced by P2Y2 receptor stimulation in cells with blocked ROCK correlates in time with actin cytoskeleton reorganization, F-actin redistribution and stress fibers assembly followed by recovery of normal cell morphology. Presented results indicate that myosin light-chain kinase (MLCK) is responsible for the observed phosphorylation of MLC. We also found that the changes induced by P2Y2 stimulation in actin cytoskeleton dynamics and morphology of cells with inhibited ROCK, but not in the level of phosphorylated MLC, depend on the presence of calcium in the cell environment.
Źródło:
Acta Biochimica Polonica; 2011, 58, 1; 125-130
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Starches Modified by Combination of Phosphorylation and High-Voltage Electrical Discharge (HVED) Treatment
Autorzy:
Grgić, Ivanka
Grec, Marijana
Gryszkin, Artur
Zięba, Tomasz
Kopjar, Mirela
Ačkar, Đurđica
Jozinović, Antun
Miličević, Borislav
Zavadlav, Sandra
Babić, Jurislav
Powiązania:
https://bibliotekanauki.pl/articles/1363283.pdf
Data publikacji:
2021
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
cereal starch
tuber starch
HVED
phosphorylation
physicochemical properties
Opis:
Starch is extensively used in the food industry as a texture modifier, a fat substitute, and in other applications. To optimise starch functional properties for specific use, it is subjected to various modifications. High-voltage electrical discharge (HVED) treatment, as a non-thermal and rapid process, was applied in this research as a single method and in combination with phosphorylation in order to explore its potential for improving starch physicochemical properties. Maize, wheat, potato, and tapioca starches were modified, and Na5P3O10 and Na2HPO4 were used for phosphorylation. Starch gelatinisation parameters (by DSC); paste clarity; and contents of amylose, damaged starch, and resistant starch were determined; and FTIR-ATR spectra were recorded. All modifications reduced the enthalpy of gelatinisation and decreased contents of amylose, resistant starch, and damaged starch. The effect of the HVED treatment on starch properties depended on starch type and combinations with chemicals. HVED could act as an aid in the starch phosphorylation process since the properties analysed were more effectively improved when HVED was combined with phosphorylation than by phosphorylation alone.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2021, 71, 1; 79-88
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Binding of Aedes aegypti trypsin modulating oostatic factor [Aea-TMOF] to its receptor stimulates phosphorylation and protease processing of gut-membrane proteins
Autorzy:
Borovsky, D.
Hamdaoui, A.
Powiązania:
https://bibliotekanauki.pl/articles/55079.pdf
Data publikacji:
2008
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
protease processing
Aedes aegypti
electrophoresis
trypsin modulating oostatic factor
phosphorylation
mosquito
insect
larva
gut-membrane protein
fluorography
gut receptor
Opis:
The binding of TMOF to its gut receptor was followed by incubating guts removed from male and female Aedes aegypti. TMOF at physiological concentrations, in the presence of [γ32P]ATP, causes phosphorylation and release of gut-membrane protein (45 kDa) that is further processed by proteolysis. In the presence of protease inhibitors only the 45 kDa protein was released. The phosphorylation and processing of the 45 kDa protein does not happen in the absence of TMOF. Both larvae and adult guts release the protein in the presence of TMOF. Male Ae. aegypti do not synthesize trypsin in their gut and do not release the 45 kDa protein in the presence of TMOF because a TMOF receptor is probably absent. Homogenized guts do not release the 45 kDa protein, indicating that the protease processing or the ecto-protein kinase activity is probably reduced after breaking the tissue. The 45 kDa phosphorylated protein can be dephosphorylated by alkaline phosphatase and protein phosphatase, indicating that the phosphate group is covalently linked to either a serine or a tyrosine moiety. This is the first report that shows that in insects, binding of a peptide hormone activates its receptor by proteolysis.
Źródło:
Pestycydy; 2008, 1-2; 13-25
0208-8703
Pojawia się w:
Pestycydy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Liver mitochondria and insulin resistance
Autorzy:
Vial, Guillaume
Dubouchaud, Hervé
Leverve, Xavier
Powiązania:
https://bibliotekanauki.pl/articles/1040285.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
oxidative phosphorylation
insulin resistance
mitochondria
membrane potential
Opis:
With a steadily increasing prevalence, insulin resistance (IR) is a major public health issue. This syndrome is defined as a set of metabolic dysfunctions associated with, or contributing to, a range of serious health problems. These disorders include type 2 diabetes, metabolic syndrome, obesity, and non-alcoholic steatohepatitis (NASH). According to the literature in the field, several cell types like β-cell, myocyte, hepatocyte and/or adipocyte, as well as related complex signaling environment involved in peripheral insulin sensitivity are believed to be central in this pathology. Because of the central role of the liver in the whole-body energy homeostasis, liver insulin sensitivity and its potential relationship with mitochondrial oxidative phosphorylation appear to be crucial. The following short review highlights how liver mitochondria could be implicated in IR and should therefore be considered as a specific therapeutic target in the future.
Źródło:
Acta Biochimica Polonica; 2010, 57, 4; 389-392
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
IAA and BAP affect protein phosphorylation-dependent processes during sucrose-mediated G1 to S and G2 to M transitions in root meristem cells of Vicia faba
Autorzy:
Polit, J T
Maszewski, J.
Rosiak, M.
Powiązania:
https://bibliotekanauki.pl/articles/58127.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Vicia faba
cell cycle
sucrose
auxin
protein phosphorylation
cytokinin
control point
meristem
root
Opis:
In carbohydrate-starved root meristems of Vicia faba subsp. minor, the expression of two Principal Control Points located at the final stages of the G1 (PCP1) and G2 (PCP2) phases has been found to be correlated with a marked decrease of protein phosphorylation within cell nuclei, nucleoli and cytoplasm. Adopting the same experimental model in our present studies, monoclonal FITC conjugated antibodies that recognize phosphorylated form of threonine (αTPab-FITC) were used to obtain an insight about how the indole-3-acetic acid (IAA), benzyl-6-aminopurine (BAP), and the mixture of both phytohormones influence the time-course changes in an overall protein phosphorylation during sucrose-mediated PCP1→S and PCP2→M transitions. Unsuspectedly, neither IAA, BAP, nor the mixture of both phytohormones supplied in combination with sucrose did up-regulate protein phosphorylation. However using the block-and-release method, it was shown that root meristems of Vicia provided with sucrose alone indicated higher levels of αTPab-FITC. Contrarily, phytohormones supplied in combination with sucrose induced apparent decline in phosphorylation of cell proteins, which - when compared with the influence of sucrose alone - became increasingly evident in time. Thus, it seems probable, that a general decline in the amount of αTPab-FITC labeled epitopes may overlay specific phosphorylations and dephosphorylations governed by the main cell cycle kinases and phosphatases.
Źródło:
Acta Societatis Botanicorum Poloniae; 2004, 73, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
"MitoTea": Geranium robertianum L. decoctions decrease blood glucose levels and improve liver mitochondrial oxidative phosphorylation in diabetic Goto-Kakizaki rats
Autorzy:
Ferreira, Fernanda
Peixoto, Francisco
Nunes, Elsa
Sena, Cristina
Seiça, Raquel
Santos, Maria
Powiązania:
https://bibliotekanauki.pl/articles/1040293.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
oxidative phosphorylation
herbal medicine
Vaccinium myrtillus L.
Goto-Kakizaki (GK) rats
type 2 diabetes mellitus
Geranium robertianum L.
Opis:
Several chemical compounds found in plant products have proven to possess beneficial properties, being currently pointed out due to their pharmacological potential in type 2 diabetes mellitus complications. In this context, we studied the effect of Geranium robertianum L. (herb Robert) leaf decoctions in Goto-Kakizaki (GK) rats, a model of type 2 diabetes. Our results showed that oral administration of G. robertianum leaf decoctions over a period of four weeks lowered the plasma glucose levels in diabetic rats. Furthermore, the treatment with G. robertianum extracts improved liver mitochondrial respiratory parameters (state 3, state 4 and FCCP-stimulated respiration) and increased oxidative phosphorylation efficiency.
Źródło:
Acta Biochimica Polonica; 2010, 57, 4; 399-402
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Short-term regulation of the mammalian pyruvate dehydrogenase complex
Autorzy:
Strumiło, Sławomir
Powiązania:
https://bibliotekanauki.pl/articles/1041314.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
pyruvate dehydrogenase complex
thiamine diphosphate
phosphorylation
dephosphorylation
regulation
allosteric effect
Opis:
In this minireview the main mechanism of control of mammalian pyruvate dehydrogenase complex (PDHC) activity by phosphorylation-dephosphorylation is presented in the first place. The information recently obtained in several laboratories includes new data about isoforms of the PDH converting enzymes (kinase and phosphatase) and their action in view of short-term regulation of PDHC. Moreover, interesting influence of exogenous thiamine diphosphate (TDP) and some divalent cations, especially Mn2+, on the kinetic parameters of PDHC saturated with endogenous tightly bound TDP, is discussed. This influence causes a shortening of the lag-phase of the catalyzed reaction and a strong decrease of the Km value of PDHC mainly for pyruvate. There are weighty arguments that the effects have an allosteric nature. Thus, besides reversible phosphorylation, also direct manifold increase of mammalian PDHC affinity for the substrate by cofactors seems an important aspect of its regulation.
Źródło:
Acta Biochimica Polonica; 2005, 52, 4; 759-764
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phosphorylation of yeast ribosomal proteins by CKI and CKII in the presence of heparin
Autorzy:
Wojda, Iwona
Cytryńska, Małgorzata
Frajnt, Magdalena
Jakubowicz, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/1044559.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1999, 46, 1; 211-215
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of phosphorylation and succinilation of yeast protein on its enzymatic digestibility and functional properties
Wpływ fosforylacji i sulecynylacji białka drożdżowego na jego strawność enzymatyczną i właściwości funkcjonalne
Autorzy:
Giec, A.
Stasińska, B.
Lampart-Szczapa, E.
Skupin, J.
Powiązania:
https://bibliotekanauki.pl/articles/1399535.pdf
Data publikacji:
1989
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
protein of the yeast Torula utilis
protein phosphorylation
protein succinilation
functional properties
enzymatic digestibility
Opis:
It was found that succinilation and phosphorylation of protein lead to its greater yield from yeast compared to the traditional alkaline extraction, causing a more effective reduction of nucleic acids content in the final product and a considerable improvement of the functional properties of isolated substances. At the same time phosphorylation caused a smaller reduction of enzymatic digestibility of the protein accompanying all the advantageous effects.
Włączenie białka mikrobiologicznego do żywności wymaga dostosowania jego właściwości fizykochemicznych. W literaturze opisano wiele metod osiągnięcia tego celu dzięki różnym modyfikacją chemicznym. Brak jest analizy porównawczej ich skuteczności oraz wpływu na wartość biologiczną uzyskanych preparatów białkowych. Celem niniejszej pracy było porównanie efektywności zabiegów sulecynylacji i fosforylacji białka drożdżowego z tradycyjną estrakcją alkaliczną po hydrolizie kwa sów nukleinowych. Badania wykazały że proces sulecynylacji oraz fosforylacji zwiększa ekstraktywność białka wskutek przesunięcia punktu izoelektrycznego, co wpływa istotnie na obniżenie zawartości kwasów nukleinowych w produkcie o ok. 60 % i poprawę właściwości funkcjonalnych (tabela). Fosforylacja w znacznie mniejszym topniu obniżyła strawność enzymatyczną preparatu niż sulecynylacja, a dodatkowo z uwagi na swą odwracalność wydaje się być godną polecenia metodą uzyskiwania z homogenatów drożdżowych białka przeznaczonego do żywności.
Źródło:
Acta Alimentaria Polonica; 1989, 15(39), 1; 63-66
0137-1495
Pojawia się w:
Acta Alimentaria Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro phosphorylation of ex-preprotachykinin by protein kinase C
Autorzy:
Hrabec, Elżbieta
Hrabec, Zbigniew
Lachowicz, Lilla
Greger, Janusz
Płucienniczak, Grażyna
Płucienniczak, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1045392.pdf
Data publikacji:
1994
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1994, 41, 2; 194-195
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dinitrofenol – mieszanina izomerów. Dokumentacja proponowanych wartości dopuszczalnych wielkości narażenia zawodowego
Dinitrophenol
Autorzy:
Gralewicz, S.
Powiązania:
https://bibliotekanauki.pl/articles/137927.pdf
Data publikacji:
2005
Wydawca:
Centralny Instytut Ochrony Pracy
Tematy:
dinitrofenol
fosforylacja oksydatywna
metabolizm
zaćma
dinitrophenol
oxidative phosphorylation
metabolism
cataract
Opis:
Dinitrofenol (DNP) – mieszanina izomerów: 2,3- DNP, 2,4- DNP i 2,6-DNP z przewagą 2,4-DNP, jest stosowany w produkcji barwników, kwasu pikrynowego i pikraminowego, wywoływaczy fotograficznych i materiałów wybuchowych oraz jako pestycyd w rolnictwie i sadownictwie. Dinitrofenol jest trucizną metaboliczną, a mechanizm jego działania toksycznego polega na rozprzęganiu fosforylacji oksydatywnej. Zawodowe narażenie na pary i pyły 2,4-DNP może wywoływać objawy wzmożonego metabolizmu. 2,4-DNP nie jest kancerogenem, nie wykazuje także działania genotoksycznego ani mutagennego. Za podstawę wyliczenia wartości NDS dla 2,4-DNP przyjęto wartość LOAEL dla skutków metabolicznych. Wartość ta u człowieka wynosi 1,2 mg/kg/dzień. W warunkach narażenia drogą oddechową taką dawkę pracownik może wchłonąć, gdy stężenie 2,4-DNP we wdychanym powietrzu wynosi 10,5 mg/m3. Przyjmując współczynnik niepewności równy 16 (2 dla różnic we wrażliwości osobniczej, 2 dla przejścia z wartości LOAEL do wartości NOAEL, 2 dla różnicy w sposobie narażenia i 2 dla ekstrapolacji z narażenia średnioterminowego do przewlekłego), to wyliczona wartość normatywu będzie wynosiła 0,66 mg/m3. W związku z powyższym proponuje się przyjęcie stężenia 0,5 mg/m3 za wartość NDS dla 2,4-DNP. Przyjmując, że 2,4-DNP jest najbardziej toksycznym izomerem dinitrofenolu oraz, że udział tego izomeru w dinitrofenolu – mieszaninie izomerów jest dominujący, proponujemy przyjęcie dla dinitrofenolu – mieszaniny również takiej samej wartości NDS jaką zaproponowano dla 2,4-DNP.
Dinitrophenol (DNP) is a mixture of 2,4-DNP and smaller amounts of 2,3-DNP and 2,6-DNP). It is a yellow, crystalline solid. DNP is used in synthesis of dyes, picric acid, picramic acid, wood preservatives, photographic developers, explosives, and insecticides. In the 1930s, 2,4-DNP was used as a weight-reducing drug. Short-term exposure to DNP may affect metabolism resulting in hyperthermia. High-level exposure may be fatal. The existing data concerning the health effects of 2,4-DNP oral exposure in humans indicate that the characteristic effects of 2,4-DNP for this route are: increased basal metabolic rate and perspiration, weight loss, a sensation of warmth, and – at higher dosage – increased heart and respiratory rate, and increased body temperature. Repeated or prolonged contact with the skin may cause dermatitis. Exposure to DNP may result in changes in the functional state of the peripheral nervous system, cardiovascular system and gastrointestinal system. It may also induce cataracts. Taking into account the results obtained in clinical studies on people ingesting 2,4-DNP (LOAEL for metabolic effects was 1.2 mg/kg/day), a concentration 0.5 mg of dinitrophenol/m3 is proposed as a maximum exposure limit (maximum admissible concentration) with a skin notation. With regard to systemic effects of DNP no STEL value has beeen established.
Źródło:
Podstawy i Metody Oceny Środowiska Pracy; 2005, 1 (43); 67-89
1231-868X
Pojawia się w:
Podstawy i Metody Oceny Środowiska Pracy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rabbit muscle fructose-1,6-bisphosphatase is phosphorylated in vivo.
Autorzy:
Rakus, Dariusz
Zarzycki, Marek
Dzugaj, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1043652.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
aldolase
muscle
phosphorylation
AMP
fructose-1,6-bisphosphatase
Opis:
Phosphorylated fructose-1,6-bisphosphatase (FBPase) was isolated from rabbit muscle in an SDS/PAGE homogeneous form. Its dephosphorylation with alkaline phosphatase revealed 2.8 moles of inorganic phosphate per mole of FBPase. The phosphorylated FBPase (P-FBPase) differs from the dephosphorylated enzyme in terms of its kinetic properties like Km and kcat, which are two times higher for the phosphorylated FBPase, and in the affinity for aldolase, which is three times lower for the dephosphorylated enzyme. ephosphorylated FBPase can be a substrate for protein kinase A and the amount of phosphate incorporated per FBPase monomer can reach 2-3 molecules. Since interaction of muscle aldolase with muscle FBPase results in desensitisation of the latter toward AMP inhibition (Rakus & Dzugaj, 2000, Biochem. Biophys. Res. Commun. 275, 611-616), phosphorylation may be considered as a way of muscle FBPase activity regulation.
Źródło:
Acta Biochimica Polonica; 2003, 50, 1; 115-121
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phosphorylation of acidic ribosomal proteins by ribosome-associated protein kinases of Saccharomyces cerevisiae and Schizosaccharofnyces pombe
Autorzy:
Jakubowicz, Teresa
Cytryńska, Małgorzata
Kowalczyk, Wiktor
Gąsior, Eugeniusz
Powiązania:
https://bibliotekanauki.pl/articles/1045441.pdf
Data publikacji:
1993
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1993, 40, 4; 497-505
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of cholesterol and sphigomyelin in tyrosine phosphorylation of proteins and capping of Fcγ receptor II
Autorzy:
Drzewiecka, Anna
Kwiatkowska, Katarzyna
Sobota, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1044547.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1999, 46, 1; 107-116
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Possible Involvement of cAMP and Protein Phosphorylation in the Cell Signaling Pathway for Resting Cyst Formation of Ciliated Protozoan Colpoda cucullus
Autorzy:
Sogame, Y
Asami, H.
Powiązania:
https://bibliotekanauki.pl/articles/763744.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Opis:
Possible Involvement of cAMP and Protein Phosphorylation in the Cell Signaling Pathway for Resting Cyst Formation of Ciliated Protozoan Colpoda cucullus
Źródło:
Acta Protozoologica; 2011, 50, 2
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of dual specificity protein kinase from maize seedlings.
Autorzy:
Trojanek, Joanna
Klimecka, Maria
Fraser, Anna
Dobrowolska, Grażyna
Muszyńska, Grażyna
Powiązania:
https://bibliotekanauki.pl/articles/1041540.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
dual specificity kinase
tyrosine phosphorylation
maize
Opis:
A protein kinase of 57 kDa, able to phosphorylate tyrosine in synthetic substrates pol(Glu4,Tyr1) and a fragment of Src tyrosine kinase, was isolated and partly purified from maize seedlings (Zea mays). The protein kinase was able to phosphorylate exogenous proteins: enolase, caseins, histones and myelin basic protein. Amino acid analysis of phosphorylated casein and enolase, as well as of phosphorylated endogenous proteins, showed that both Tyr and Ser residues were phosphorylated. Phosphotyrosine was also immunodetected in the 57 kDa protein fraction. In the protein fraction there are present 57 kDa protein kinase and enolase. This co-purification suggests that enolase can be an endogenous substrate of the kinase. The two proteins could be resolved by two-dimensional electrophoresis. Specific inhibitors of typical protein-tyrosine kinases had essentially no effect on the activity of the maize enzyme. Staurosporine, a nonspecific inhibitor of protein kinases, effectively inhibited the 57 kDa protein kinase. Also, poly L-lysine and heparin inhibited tyrosine phosphorylation by 57 kDa maize protein kinase. The substrate and inhibitor specificities of the 57 kDa maize protein kinase phosphorylating tyrosine indicate that it is a novel plant dual-specificity protein kinase.
Źródło:
Acta Biochimica Polonica; 2004, 51, 3; 635-647
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
40S subunit Met-tRNAi_f complexes and initiation factor elF-2 phosphorylation in mammalian cells accumulating uncharged tRNA
Autorzy:
Jakubowicz, Teresa
Frielle, Debra
Vaughan, Maurice
Powiązania:
https://bibliotekanauki.pl/articles/1045979.pdf
Data publikacji:
1985
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1985, 32, 3; 199-210
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of phosphorylation of the EF-2 isolated from rat liver cells on protein biosynthesis in vitro
Autorzy:
Gajko, Anna
Średzińska, Krystyna
Marcinkiewicz, Cezary
Gałasiński, Władysław
Powiązania:
https://bibliotekanauki.pl/articles/1045707.pdf
Data publikacji:
1991
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1991, 38, 3; 353-358
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Human mitochondria in health, disease, ageing and cancer
Autorzy:
Bartnik, E
Lorenc, A.
Mroczek, K.
Powiązania:
https://bibliotekanauki.pl/articles/2041927.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
health
oxidative phosphorylation
disease
genetics
mitochondrion
man
human cell
aging
metabolic process
cancer
Źródło:
Journal of Applied Genetics; 2001, 42, 1; 65-71
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cloning and characterization of the gene encoding ribosomal P0 phosphoprotein from Neurospora crassa.
Autorzy:
Krokowski, Dawid
Tchórzewski, Marek
Grankowski, Nikodem
Powiązania:
https://bibliotekanauki.pl/articles/1043800.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phosphorylation
cloning
ribosomal P0 protein
Neurospora crassa
Opis:
A gene for ribosomal protein P0 that belongs to the family of ribosomal P proteins was isolated from a Neurospora crassa cDNA library, using polyclonal antibodies against recombinant P0 protein from Saccharomyces cerevisiae. This is the first gene for ribosomal P0 protein to be cloned from filamentous fungi. The derived P0 protein sequence has a strong homology to other eukaryotic P0 proteins; yet, there is a notable alteration in the conservative C-terminal region, placing this protein among the unique sequences from protozoan parasites.
Źródło:
Acta Biochimica Polonica; 2002, 49, 1; 11-18
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Advantage of a baculovirus expression system for protein-protein interaction studies. Involvement of posttranslational phosphorylation in the interaction between wt p53 protein and poly(ADP-ribose) polymerase-1
Autorzy:
Schmid, Gerald
Wojciechowski, Jacek
Węsierska-Gądek, Józefa
Powiązania:
https://bibliotekanauki.pl/articles/1041382.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
nucleocytoplasmic shuttling
NES
p53 isomers
p53 stability
cell cycle arrest
2D-PAGE
p53 nuclear export
FACS analysis
p53 pull-down assay
p53 phosphorylation
Opis:
We recently observed an interaction between poly(ADP-ribose) polymerase-1 (PARP-1) and the tumor suppressor p53 protein. However, more extensive studies on both proteins, especially those on characterization of their domains involved in the interaction were difficult due to very low expression levels of p53 in mammalian cells. Therefore, we generated recombinant proteins for such studies. To clarify which domains of human PARP-1 and of human wild-type (wt) p53 were involved in this protein-protein interaction, we generated baculoviral constructs encoding full length or distinct functional domains of both proteins. Full length PARP-1 was simultaneously coexpressed in insect cells with full length wt p53 protein or its distinct truncated fragments and vice versa. Reciprocal immunoprecipitation of Sf9 cell lysates revealed that the central and carboxy-terminal fragments of p53 each were sufficient to confer binding to PARP-1, whereas the amino-terminal part harbouring the transactivation functional domain was dispensable. On the other hand, the amino-terminal and central fragments of PARP-1 were both necessary for complex formation with p53 protein. Since the most important features of p53 protein are regulated by phosphorylation, we addressed the question whether its phosphorylation is essential for the binding between the two proteins. Baculovirally expressed wt p53 was post-translationally modified. At least six distinct p53 isomers were resolved by immunoblotting following two-dimensional separation of baculovirally expressed wt p53 protein. Using specific phospho-serine antibodies, we identified phosphorylation of baculovirally expressed p53 protein at five distinct sites. To define the role of p53 phosphorylation, pull-down assays using untreated and dephosphorylated p53 protein were performed. Dephosphorylated p53 failed to bind PARP-1, indicating that complex formation between the two proteins was regulated by phosphorylation of p53. The marked phosphorylation of p53 at Ser392 observed in unstressed cells suggests that the phosphorylated carboxy-terminal part of p53 undergoes complex formation with PARP-1 resulting in masking of the NES and thereby preventing its export.
Źródło:
Acta Biochimica Polonica; 2005, 52, 3; 713-719
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The phosphorylation sites of ribosomal P proteins from Saccharomyces cerevisiae cells by endogenous CK-2, PK60S and RAP protein kinases
Autorzy:
Boguszewska, Aleksandra
Szyszka, Ryszard
Grankowski, Nikodem
Powiązania:
https://bibliotekanauki.pl/articles/1044936.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1997, 44, 2; 191-200
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differential phosphorylation of ribosomal acidic proteins from yeast cell by two endogenous protein kinases: casein kinase-2 and 60S kinase
Autorzy:
Szyszka, Ryszard
Boguszewska, Aleksandra
Grankowski, Nikodem
Ballesta, Juan
Powiązania:
https://bibliotekanauki.pl/articles/1045228.pdf
Data publikacji:
1995
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1995, 42, 3; 357-362
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vivo phosphorylation of alloxymethyl purine and pyrimidine acyclonucleosides and the inhibitory effect of these compounds on thymidine and deoxyguanosine kinases
Autorzy:
Modrzejewska, Hanna
Greger, Janusz
Lewandowska, Urszula
Fidek, Weronika
Powiązania:
https://bibliotekanauki.pl/articles/1045389.pdf
Data publikacji:
1994
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1994, 41, 2; 185-187
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prp4 kinase is required for proper segregation of chromosomes during meiosis in Schizosaccharomyces pombe
Autorzy:
Pozgajova, Miroslava
Cipak, Lubos
Trakovicka, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1039511.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Prp4 protein kinase
S. pombe
meiosis
segregation
protein phosphorylation
Opis:
Chromosome segregation during meiosis is a complex process, which leads to production of four haploid gametes from two precursor cells. Reversible phosphorylation of proteins plays a crucial role in this process. The Schizosaccharomyces pombe Prp4 is an essential serine/threonine protein kinase, which belongs to the Clk/Sty family. To study the role of Prp4 in meiosis, we analysed chromosome segregation in a strain carrying conditional analog-sensitive allele of Prp4 protein kinase (prp4-as2). Our data show, that Prp4 protein kinase plays important role in chromosome segregation during meiosis, as revealed by enhanced missegregation of chromosomes in prp4-as2 mutant cells.
Źródło:
Acta Biochimica Polonica; 2013, 60, 4; 871-873
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modulation of the human preadipocyte mitochondrial activity by beta-carotene
Autorzy:
Śliwa, Agnieszka
Góralska, Joanna
Czech, Urszula
Gruca, Anna
Polus, Anna
Zapała, Barbara
Dembińska-Kieć, Aldona
Powiązania:
https://bibliotekanauki.pl/articles/1039768.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
oxidative phosphorylation
preadipocytes
mitochondria
beta-carotene
Opis:
Increased ROS generation by the overload by metabolic substrates mitochondria paralleled by decrease of antioxidant activity are typical events found in metabolic syndrome and diabetes type 2. Metabolites of beta-carotene (BC) such as retinoic acid (RA), as well as low concentration of reactive oxygen species (ROS) modify the mitochondrial bioenergetic function. The aim of the study was to investigate the effect of beta-carotene on mitochondrial activity in human preadipocytes. BC used in concentrations, 10 or 30 µM, decreased mitochondrial membrane potential, inhibited mitochondrial respiration and decreased cellular ATP content. We conclude, that BC, the known antioxidant may decrease oxidative phosphorylation capacity of mitochondria.
Źródło:
Acta Biochimica Polonica; 2012, 59, 1; 39-41
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
High sequence homology between protein tyrosine acid phosphatase from boar seminal vesicles and human prostatic acid phosphatase
Autorzy:
Wysocki, Paweł
Płucienniczak, Grażyna
Strzeżek, Jerzy
Powiązania:
https://bibliotekanauki.pl/articles/1040544.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phosphorylation
boar
protein tyrosine phosphatase
seminal plasma
prostatic acid phosphatase
Opis:
Boar seminal vesicle protein tyrosine acid phosphatase (PTAP) and human prostatic acid phosphatase (PAP) show high affinity for protein phosphotyrosine residues. The physico-chemical and kinetic properties of the boar and human enzymes are different. The main objective of this study was to establish the nucleotide sequence of cDNA encoding boar PTAP and compare it with that of human PAP cDNA. Also, the amino-acid sequence of boar PTAP was compared with the sequence of human PAP. PTAP was isolated from boar seminal vesicle fluid and sequenced. cDNA to boar seminal vesicle RNA was synthesized, amplified by PCR, cloned in E. coli and sequenced. The obtained N-terminal amino-acid sequence of boar PTAP showed 92% identity with the N-terminal amino-acid sequence of human PAP. The determined sequence of a 354 bp nucleotide fragment (GenBank accession number: GQ184596) showed 90% identity with the corresponding sequence of human PAP. On the basis of this sequence a 118 amino acid fragment of boar PTAP was predicted. This fragment showed 89% identity with the corresponding fragment of human PAP and had a similar hydropathy profile. The compared sequences differ in terms of their isoelectric points and amino-acid composition. This may explain the differences in substrate specificity and inhibitor resistance of boar PTAP and human PAP.
Źródło:
Acta Biochimica Polonica; 2009, 56, 3; 481-486
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inhibition of mitochondrial bioenergetics: the effects on structure of mitochondria in the cell and on apoptosis.
Autorzy:
Lyamzaev, Konstantin
Izyumov, Denis
Avetisyan, Armine
Yang, Fuyu
Pletjushkina, Olga
Chernyak, Boris
Powiązania:
https://bibliotekanauki.pl/articles/1043303.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
oxidative phosphorylation
inhibitors
mitochondria
apoptosis
Opis:
The effects of specific inhibitors of respiratory chain, FoF1ATP synthase and uncouplers of oxidative phosphorylation on survival of carcinoma HeLa cells and on the structure of mitochondria in the cells were studied. The inhibitors of respiration (piericidingg, antimycin, myxothiazol), the F1-component of ATP synthase (aurovertin) and uncouplers (DNP, FCCP) did not affect viability of HeLa cells, apoptosis induced by TNF or staurosporin and the anti-apoptotic action of Bcl-2. Apoptosis was induced by combined action of respiratory inhibitors and uncouplers indicating possible pro-apoptotic action of reactive oxygen species (ROS) generated by mitochondria. Short-term incubation of HeLa cells with the mitochondrial inhibitors and 2-deoxyglucose followed by 24-48 h recovery resulted in massive apoptosis. Apoptosis correlated to transient (3-4 h) and limited (60-70%) depletion of ATP. More prolonged or more complete transient ATP depletion induced pronounced necrosis. The inhibitors of respiration and uncouplers caused fragmentation of tubular mitochondria and formation of small round bodies followed by swelling. These transitions were not accompanied with release of cytochrome c into the cytosol and were fully reversible. The combined effect of respiratory inhibitors and uncouplers developed more rapidly indicating possible involvement of ROS generated by mitochondria. More prolonged (48-72 h) incubation with this combination of inhibitors caused clustering and degradation of mitochondria.
Źródło:
Acta Biochimica Polonica; 2004, 51, 2; 553-562
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Protein phosphatase 2A: Variety of forms and diversity of functions
Autorzy:
Lechward, Katarzyna
Awotunde, Olubunmi
Świątek, Wojciech
Muszyńska, Grażyna
Powiązania:
https://bibliotekanauki.pl/articles/1044037.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
protein phosphatase 2A
signal transduction
protein-protein interactions
reversible phosphorylation
cell cycle
carcinogenesis.
Opis:
Protein phosphatase 2A (PP2A) comprises a diverse family of phosphoserine-and phosphothreonine-specific phosphatases present in all eukaryotic cells. All forms of PP2A contain a catalytic subunit (PP2Ac) which forms a stable complex with the structural subunit PR65/A. The heterodimer PP2Ac-PR65/A associates with regulatory proteins, termed variable subunits, in order to form trimeric holoenzymes attributed with distinct substrate specificity and targeted to different subcellular compartments. PP2Ac activity can be modulated by reversible phosphorylation on Tyr307 and methylation on C-terminal Leu309. Studies on PP2A have shown that this enzyme may be implicated in the regulation of metabolism, transcription, RNA splicing, translation, differentiation, cell cycle, oncogenic transformation and signal transduction.
Źródło:
Acta Biochimica Polonica; 2001, 48, 4; 921-933
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Protein kinases CKI and CKII are implicated in modification of ribosomal proteins of the yeast Trichosporon cutaneum
Autorzy:
Wojda, Iwona
Cytryńska, Małgorzata
Frajnt, Magdalena
Jakubowicz, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/1043700.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
CKII
CKI
phosphorylation
ribosomal phosphoproteins
Trichosporon cutaneum
Opis:
Phosphorylation of acidic ribosomal proteins P1/P2-P0 is a common phenomenon in eukaryotic organisms. It was found previously that in Trichosporon cutaneum, unlike in other yeast species, in addition to the two acidic ribosomal proteins, two other proteins of 15 kDa and 19 kDa of the small ribosomal subunit were phosphorylated. Here we describe two protein kinases: CKI and CKII, which are engaged in the modification of T. cutaneum ribosomal proteins. The acidic ribosomal proteins and the protein of 19 kDa were modified by CKII associated with ribosomes, while the protein of 15 kDa was modified by CKI. Protein kinase CKI was purified from cell-free extract (CKIC) and from ribosomal fraction (CKIR). The molecular mass of CKIC was established at 33 kDa while that of CKIR at 35-37 kDa. A protein of 40 kDa copurified with CKIR but not CKIC. Heparin significantly increased 40 kDa protein phosphorylation level by CKIR. Microsequencing analysis revealed the presence of CKI recognition motifs in the N-terminal fragment of the 40 kDa protein.
Źródło:
Acta Biochimica Polonica; 2002, 49, 4; 947-957
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
SDS/PAGE characteristics of protein kinases tightly associated with chick embryo brain ribosomes
Autorzy:
Sanecka-Obacz, Maria
Powiązania:
https://bibliotekanauki.pl/articles/1044449.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
protein phosphorylation
chick brain development
ribosomal kinases
CK2
PKA
CK1
fetal brain
brain ribosomes
Opis:
Protein kinases tightly associated with chick embryo brain ribosomes washed with Triton X-100 and KCl were characterized by their ability to phosphorylate ribosomes and two exogenous substrates, histone IIA and casein. c-AMP-dependent kinase (PKA) and casein kinases (CK1, CK2) were examined in the presence of specific modulators by SDS/ PAGE followed by  renaturation in gel assay according to Kameshita & Fujisawa (Anal. Biochem. 1989, 183, 139-143). Basing on these data it can be presumed that PKA activity increases, but the levels of CK2 and CK1 decrease during chick embryo development.
Źródło:
Acta Biochimica Polonica; 1999, 46, 4; 911-917
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of cAMP and cGMP on the activity and substrate specificity of protein kinase A from methylotrophic yeast Pichia pastoris.
Autorzy:
Frajnt, Magdalena
Cytryńska, Małgorzata
Jakubowicz, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/1043395.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
protein phosphorylation
cAMP
PKA
cGMP
yeast
Opis:
Cyclic AMP dependent protein kinase (PKA) from Pichia pastoris yeast cells was found to be activated by either cAMP or cGMP. Analogs of cAMP such as 8-chloro-cAMP and 8-bromo-cAMP were as potent as cAMP in PKA activation while N6,2'-O-dibutyryl-cAMP did not stimulate the enzyme activity. It was shown that protamine sulfate was almost equally phosphorylated in the presence of 1-2 × 10-6 M cAMP or cGMP while other substrates such as Kemptide, ribosomal protein S6, were phosphorylated to a lower extent in the presence of cGMP. It was demonstrated that pyruvate kinase is a substrate of PKA which co-purified with the P. pastoris enzyme. Moreover, pyruvate kinase was phosphorylated by PKA in the presence of cAMP and cGMP to comparable levels.
Źródło:
Acta Biochimica Polonica; 2003, 50, 4; 1111-1118
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Catalytic activity of mutants of yeast protein kinase CK2α
Autorzy:
Sajnaga, Ewa
Kubiński, Konrad
Szyszka, Ryszard
Powiązania:
https://bibliotekanauki.pl/articles/1040685.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
protein phosphorylation
ATP binding
mutagenesis
protein kinase CK2
ATP-competitive inhibitors
Saccharomyces cerevisiae
spermine
heparin
yeast
Opis:
Yeast CK2 is a highly conserved member of the protein kinase CGMC subfamily composed of two catalytic (α and α') and two regulatory (β and β') subunits. The amino-acid sequences of both catalytic subunits are only 60% homologous. Modelling of the tertiary structure of the CK2α displays additional α-helical structures not present in the CK2α' subunit, connecting the ATP-binding loop with the catalytic and activation loops. Deletion of this part causes drastic structural and enzymatic changes of the protein (CK2αΔ91-128) with characteristics similar to yeast CK2α' (low sensitivity to salt, heparin and spermine). Additionally, the deletion causes an over 5-fold decrease of the binding affinity for ATP and ATP-competitive inhibitors (TBBt and TBBz). The structural basis for TBBt and TBBz selectivity is provided by the hydrophobic pocket adjacent to the ATP/GTP binding site, which is smaller in CK2 than in the majority of other protein kinases. The importance of hydrophobic interactions in the binding of specific inhibitors was investigated here by mutational analysis of CK2α residues whose side chains contribute to reducing the size of the hydrophobic pocket. Site-directed mutagenesis was used to replace Val67 and Ile213 by Ala. The kinetic properties of the single mutants CK2αVal67Ala and CK2αIle213Ala, and the double mutant CK2Val67Ala Ile213Ala were studied with respect to ATP, and both inhibitors TBBt and TBBz. The Km values for ATP did not change or were very close to those of the parental kinase. In contrast, all CK2α mutants analysed displayed higher Ki values towards the inhibitors (10 to 12-fold higher with TBBt and 3 to 6-fold with TBBt) comparing to recombinant wild-type CK2α.
Źródło:
Acta Biochimica Polonica; 2008, 55, 4; 767-776
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Different properties of four molecular forms of protein kinase CK2 from Saccharomyces cerevisiae
Autorzy:
Domańska, Katarzyna
Zieliński, Rafał
Kubiński, Konrad
Sajnaga, Ewa
Masłyk, Maciej
Bretner, Maria
Szyszka, Ryszard
Powiązania:
https://bibliotekanauki.pl/articles/1041353.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
isoenzyme specificity
protein phosphorylation
protein kinase CK2
yeast
Opis:
CK2 is a pleiotropic constitutively active serine/threonine protein kinase composed of two catalytic α- and two regulatory β-subunits, whose regulation is still not well understood. It seems to play an essential role in regulation of many cellular processes. Four active forms of CK2, composed of αα'ββ', α2ββ', α'2ββ', and a free α'-subunit were isolated from wild-type yeast and strains containing a single deletion of the catalytic subunit. Each species exhibits properties typical for CK2, but they differ in substrate specificity and sensitivity to inhibitors. This suggests that each CK2 isomer may regulate different process or may differ in the way of its regulation.
Źródło:
Acta Biochimica Polonica; 2005, 52, 4; 947-951
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
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