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Tytuł:
New tool microscopy technique
Autorzy:
Rybka, S.
Powiązania:
https://bibliotekanauki.pl/articles/951236.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
VS120 system
high speed scanning system
differential interference contrast microscopy
virtual microscopy
virtual slide system
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mikroskopowe metody badania cytoszkieletu
Microscopy techniques for cytoskeleton resarch
Autorzy:
Nowak, Natalia
Pomorski, Paweł
Powiązania:
https://bibliotekanauki.pl/articles/1033968.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Tematy:
cytoskeleton
electron microscopy
immunocytochemistry
molecular probes
optical microscopy
superresolution microscopy
cytoszkielet
immunocytochemia
mikroskopia elektronowa
mikroskopia optyczna
mikroskopia superrozdzielcza
sondy molekularne
Opis:
Cytoszkielet to sieć białkowych polimerów oraz związanych z nimi setek białek motorycznych, regulatorowych i łączących cytoszkielet z innymi strukturami komórkowymi. Rozwój wiedzy o cytoszkielecie jest nierozerwalnie zwiększany z postępem technik mikroskopowych używanych do jego obserwacji. Początki tych badań to niespecyficzne, nieskomplikowane barwienia utrwalonego materiału biologicznego, które później rozwinęły się w nowoczesną mikroskopię strukturalną, pozwalającą na precyzyjne znakowanie określonych białek tworzących cytoszkielet, badanie ich stanu fizjologicznego czy też oddziaływań cytoszkieletu z luźno związanymi białkami błony czy cytoplazmy. Obecnie możliwe jest nie tylko obrazowanie struktury i funkcji cytoszkieletu ze znacznie lepszą rozdzielczością przestrzenną, ale także prowadzenie tych obserwacji na żywym materiale biologicznym. Z drugiej strony, stabilność cytoszkieletu umożliwia poszukiwanie nowych metod jego obrazowania, co niewątpliwie należy do kół napędowych postępu, jaki dokonał się i wciąż dokonuje się w dziedzinie mikroskopii.
Cytoskeleton is basically a network of protein polymers, but it also contains thousands of motor, regulatory and scaffolding proteins that interact with this network. Discoveries related to the cytoskeleton were strictly connected to the development of microscopy techniques used to observe the cytoskeletal structures. At first, the imaging involved only unspecific, very simple staining of fixed material. Then, the methods evolved into advanced structural microscopy, which enabled accurate detection of specific cytoskeletal proteins, their physiological status, and interactions with loosely bound membrane and cytoplasmic proteins. Today, it is possible not only to visualize the structure and function of the cytoskeleton with better spatial resolution but also to perform the imaging in vivo on live biological specimens. On the other hand, one should also notice that observations of the stable, well defined cytoskeletal structures from their very discovery have continuously stimulated the progress in the microscopy field.
Źródło:
Kosmos; 2018, 67, 1; 219-232
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
ARMScope – the versatile platform for scanning probe microscopy systems
Autorzy:
Świadkowski, Bartosz
Piasecki, Tomasz
Rudek, Maciej
Świątkowski, Michał
Gajewski, Krzysztof
Majstrzyk, Wojciech
Babij, Michał
Dzierka, Andrzej
Gotszalk, Teodor
Powiązania:
https://bibliotekanauki.pl/articles/221551.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Scanning probe microscopy
AFM
Kelvin Probe force microscopy
scanning tunnelling microscopy
Opis:
Scanning probe microscopy (SPM) since its invention in the 80’s became very popular in examination of many different sample parameters, both in university and industry. This was the effect of bringing this technology closer to the operator. Although the ease of use opened a possibility for measurements without high labour requirement, a quantitative analysis is still a limitation in Scanning Probe Microscopes available on the market. Based on experience of Nano-metrology Group, SPM still can be considered as a tool for quantitative examination of thermal, electrical and mechanical surface parameters. In this work we present an ARMScope platform as a versatile SPM controller that is proved to be useful in a variety of applications: from atomic-resolution STM (Scanning Tunnelling Microscopy) to Multi-resonance KPFM (Kelvin Probe force microscopy) to commercial SEMs (Scanning electron microscopes).
Źródło:
Metrology and Measurement Systems; 2020, 27, 1; 119-130
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mikroskopia świetlna i konfokalna
Autorzy:
Snopiński, P.
Jarka, P.
Bilewicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/274046.pdf
Data publikacji:
2017
Wydawca:
Roble
Tematy:
badania materiałowe
mikroskopia świetlna
mikroskopia konfokalna
fluorescencyjna mikroskopia konfokalna
materials science
light microscopy
confocal microscopy
fluorescent confocal microscopy
Źródło:
LAB Laboratoria, Aparatura, Badania; 2017, 22, 6; 18-22
1427-5619
Pojawia się w:
LAB Laboratoria, Aparatura, Badania
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seebeck coefficient measurement by Kelvin-probe force microscopy
Autorzy:
Ikeda, H.
Salleh, F.
Asai, K.
Powiązania:
https://bibliotekanauki.pl/articles/384281.pdf
Data publikacji:
2009
Wydawca:
Sieć Badawcza Łukasiewicz - Przemysłowy Instytut Automatyki i Pomiarów
Tematy:
Seebeck coefficient
Kelvin-probe force microscopy
nanostructure
Opis:
In order to measure the Seebeck coefficient of nanometer-scale thermoelectric materials, we propose a new technique in which the thermoelectric-motive force (TEMF) is evaluated by Kelvin-probe force microscopy (KFM). In this study, we measured the Seebeck coefficient of an n-type Si wafer. The surface-potential difference between the high- and low-temperature regions on the Si wafer increases with increasing temperature difference. This indicates that the TEMF can be measured by KFM. The Seebeck coefficient evaluated from the surface-potential difference is 0.71š0.08 mV/K, which is close to that obtained by the conventional method.
Źródło:
Journal of Automation Mobile Robotics and Intelligent Systems; 2009, 3, 4; 49-51
1897-8649
2080-2145
Pojawia się w:
Journal of Automation Mobile Robotics and Intelligent Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Quantitative estimation of lysosomal storage in mucopolysaccharidoses by electron microscopy analysis
Autorzy:
Narajczyk, Magdalena
Moskot, Marta
Konieczna, Aleksandra
Powiązania:
https://bibliotekanauki.pl/articles/1039691.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
mucopolysaccharidoses
transmission electron microscopy
glycosaminoglycans
Opis:
Mucopolysaccharidoses (MPS) are severe inherited metabolic disorders caused by storage of glycosaminoglycans (GAGs). The level of accumulated GAGs is an important parameter in assessment of the severity of the disease and the efficacy of treatment; unfortunately, biochemical methods are often unreliable and only semi-quantitative. Therefore, finding other methods for estimation of GAG levels and/or assessment of the efficacy of applied therapy is very important. Although monitoring of GAG levels during therapy is crucial, in this work it is proposed that analysis of the ultrastructure of MPS cells by electron microscopic techniques can be considered as an alternative and reliable method for assessment of lysosomal storage. The number of complex lysosomal structures was found to be significantly higher in MPS cells relative to controls, while it decreased significantly in response to either enzyme replacement therapy or substrate reduction therapy. Thus, this parameter, easily assessed by electron microscopy, appears to correspond to the physiological state of MPS cells.
Źródło:
Acta Biochimica Polonica; 2012, 59, 4; 693-696
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phase Object Observation System Based on Diffraction Phase Microscopy
Autorzy:
Babicz, S.
Stawarz-Graczyk, B.
Wierzba, P.
Powiązania:
https://bibliotekanauki.pl/articles/221523.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
diffraction phase microscopy
DPM
phase objects
Opis:
In the paper authors present a special measurement system for observing phase objects. The diffraction phas microscopy makes it possible to measure the dimensions of a tested object with a nanometre resolution. To meet this requirement, it is proposed to apply a spatial transform. The proposed setup can be based either on a two lenses system (called 4f) or a Wollaston prism. Both solutions with all construction aspects are described in the paper. To make a full analysis of the object shape the authors developed an accurate image processing algorithm, also presented in the paper.
Źródło:
Metrology and Measurement Systems; 2018, 25, 1; 213-221
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigations of HaH 286 eucrite by analytical electron microscopy
Autorzy:
Szurgot, M.
Polański, K.
Powiązania:
https://bibliotekanauki.pl/articles/411480.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Meteorytowe
Tematy:
Eucrite
Hammadah al Hamra 286
analytical electron microscopy
meteorite
Opis:
The elemental composition, mineral composition and microstructure of the HaH 286 eucrite found in Lybia in 2000 were studied by analytical electron microscopy. It was established that the mean elemental composition of HaH 286 and atomic and molar ratios: Fe/Mn = 34, Mg/Mg+Fe = 36, Na/Al = 0.066, and Ca/Al = 0.73 are typical of eucrites, and two main meteorite minerals have the mean composition: clinopyroxene En34Fs59Wo7 and plagioclase feldspar An88Ab12. Variations in the composition of pyroxenes and plagioclases are as follows: pyroxene En34-36Fs53-62Wo3-13 and plagioclase: An86-100Ab14-0. Pyroxene is represented by pigeonite and by orthopyroxene. Chromite, ilmenite and silica are minor minerals. The composition, atomic ratios and microstructure indicate that the HaH 286 meteorite is a pyroxene-plagioclase basaltic achondrite, a non-cumulate eucrite with the composition of plagioclase changing between anorthite and bytownite.
Źródło:
Meteorites; 2011, 1, 1; 29-38
2299-0313
2299-1220
Pojawia się w:
Meteorites
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of Using Acoustic Microscopy to Evaluate Defects in Spot Welding Joints
Autorzy:
Korzeniowski, M.
Białobrzeska, B.
Powiązania:
https://bibliotekanauki.pl/articles/352373.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
resistance spot welding
acoustic microscopy
aluminium
metallography
Opis:
The article presents the possibilities of using acoustic microscopy to evaluate defects in resistance spot welding joints. For this purpose, the welded joints were made from two grades of aluminium plates EN AW5754 H24 and EN AW6005 T606, which were then subjected to non-destructive testing using acoustic microscopy and conventional destructive testing using traditional light microscopy techniques. Additionally, the study examined the influence of the typical contaminants found in industrial conditions on the quality of the joint.
Źródło:
Archives of Metallurgy and Materials; 2016, 61, 2B; 1009-1019
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of enzymatic effects on pit membranes using light and scanning electron microscopy
Autorzy:
Durmaz, S.
Yildiz, U.C.
Ozturk, M.
Serdar, B.
Powiązania:
https://bibliotekanauki.pl/articles/52338.pdf
Data publikacji:
2016
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Technologii Drewna
Tematy:
investigation
enzymatic effect
pit membrane
light electron microscopy
scanning electron microscopy
spruce wood
permeability
enzymatic treatment
Źródło:
Drewno. Prace Naukowe. Doniesienia. Komunikaty; 2016, 59, 198
1644-3985
Pojawia się w:
Drewno. Prace Naukowe. Doniesienia. Komunikaty
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Review of the advanced microscopy techniques used for diagnostics of grinding wheels with ceramic bond
Autorzy:
Kapłonek, W.
Nadolny, K.
Powiązania:
https://bibliotekanauki.pl/articles/99733.pdf
Data publikacji:
2012
Wydawca:
Wrocławska Rada Federacji Stowarzyszeń Naukowo-Technicznych
Tematy:
microscopy
abrasive tools
diagnostics
Opis:
A wide range of abrasive tools is used in modern removal machining techniques. Their condition has significant influence on the quality of the shaped surfaces of the produced elements and the functions they perform. Abrasive tools diagnostics is in this case an essential element of the production process, connected with assessment of the abrasive tool surface condition. Such an assessment can be carried out with microscopic methods The work presents selected microscopic techniques on the cutting edge of technology such as: digital microscopy, confocal laser scanning microscopy and scanning electron microscopy. Characteristics of each of the methods and the applied devices were briefly presented. The experimental part presents exemplary results of measurements and analyses carried out using the described methods. The works were carried out on, among others, grinding wheels with ceramic bond used in internal cylindrical grinding processes. The assessed tools were characterized by a number of features such as impregnated active surface, modified bond microstructure or smearing the active surface with chips of the machined material. The obtained results confirmed the possibility of application of the above-mentioned microscopic techniques in abrasive tools diagnostics.
Źródło:
Journal of Machine Engineering; 2012, 12, 4; 81-98
1895-7595
2391-8071
Pojawia się w:
Journal of Machine Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microscopic analysis of the nanostructures impact on endothelial cells
Autorzy:
Kołodziejczyk, Agnieszka Maria
Kucińska, Magdalena
Jakubowska, Aleksandra
Siatkowska, Małgorzata
Sokołowska, Paulina
Kotarba, Sylwia
Makowski, Krzysztof
Komorowski, Piotr
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/1844983.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanostructures
atomic force microscopy
transmission electron microscopy
scanning electron microscopy
cell morphology
Opis:
Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
Źródło:
Engineering of Biomaterials; 2020, 23, 154; 2-8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microscopic analysis of the nanostructures impact on endothelial cells
Autorzy:
Kołodziejczyk, Agnieszka Maria
Kucińska, Magdalena
Jakubowska, Aleksandra
Siatkowska, Małgorzata
Sokołowska, Paulina
Kotarba, Sylwia
Makowski, Krzysztof
Komorowski, Piotr
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/970974.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanostructures
atomic force microscopy
transmission electron microscopy
scanning electron microscopy
cell morphology
Opis:
Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
Źródło:
Engineering of Biomaterials; 2020, 23, 154; 2-8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of noise in surface topography measurement using structured illumination microscopy
Autorzy:
Li, Zhen
Gröger, Sophie
Powiązania:
https://bibliotekanauki.pl/articles/2052124.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
surface topography measurement
measurement noise
uncertainty
structured illumination microscopy
Opis:
Noise is a fundamental metrological characteristic of the instrument in surface topography measurement. Therefore, measurement noise should be thoroughly studied in practical measurement to understand instrument performance and optimize measurement strategy. This paper investigates the measurement noise at different measurement settings using structured illumination microscopy. The investigation shows that the measurement noise may scatter significantly among different measurement settings. Eliminating sample tilt, selecting low vertical scanning interval and high exposure time is helpful to reduce the measurement noise. In order to estimate the influence of noise on the measurement, an approach based on metrological characteristics is proposed. The paper provides a practical guide to understanding measurement noise in a wide range of applications.
Źródło:
Metrology and Measurement Systems; 2021, 28, 4; 767-779
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Atomic force microscopy investigation of electron beam (EB) irradiated composites based on biodegradable polymers and coconut fiber*
Autorzy:
Kodama, Y.
Oishi, A.
Nagasawa, N.
Nakayama, K.
Tamada, M.
Machado, L. D. B.
Powiązania:
https://bibliotekanauki.pl/articles/147470.pdf
Data publikacji:
2013
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
atomic force microscopy (AFM)
coconut fiber
electron beam (EB) radiation
force modulation microscopy (FMM)
poly(ε-caprolactone) (PCL)
poly(L-lactic acid) (PLLA)
scanning electron microscopy (SEM)
Opis:
In this study, the addition of natural fibers to biodegradable PCL, PLLA blend and the effect of ionizing radiation on the surface of composites were investigated by force modulation microscopy (FMM), atomic force microscopy (AFM) and scanning electron microscopy (SEM). Hot pressed sheets were prepared using pellets of twin extruded PCL:PLLA 20:80 (w:w) blend containing 5% or 10% weight concentration of coconut fiber. In this study coconut fibers non treated chemically and acetylated ones were used. Irradiation was performed using an electron beam (EB) accelerator and an absorbed dose of 100 kGy. FMM images acquisition was obtained using a silicon cantilever, intermittent contact mode (tapping mode). Also, AFM images were obtained using tapping mode but J scanner. By FMM, it was possible to observe regions with different elasticity indicating fiber presence under the surface of the composite. Furthermore, it appears that the spherical structures size decreased on composites. This probably was induced by ionizing radiation.
Źródło:
Nukleonika; 2013, 58, 4; 459-468
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mikroskopia konfokalna a mikroskopia szerokiego pola - dwa podejścia do badań przyżyciowych
Confocal microscopy and wide-field microscopy, two approaches to the live study
Autorzy:
Brutkowski, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1192769.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Opis:
Doświadczenia na żywych organizmach od lat były podstawą badań w naukach biologicznych. Pozwalają one na bezpośrednią obserwację mechanizmów biologicznych zachodzących w różnego rodzaju komórkach, mikrobach czy nawet w żywych zwierzętach. Dzięki rozwojowi różnego rodzaju technik fluorescencyjnych i wykorzystaniu tradycyjnej mikroskopii fluorescencyjnej czy mikroskopii konfokalnej badania przyżyciowe prowadzi się obecnie w laboratoriach na całym świecie. Rozwój technik obrazowania przyżyciowego na wielu płaszczyznach technologicznych, zaczynając od różnego rodzaju komponentów optycznych, mechanicznych czy elektronicznych, poprzez koncepcyjne podejścia do tego typu badań, a kończąc na tworzeniu nowych znaczników fluorescencyjnych, których właściwości chemiczne są dostosowane do konkretnych doświadczeń, pozwolił na obserwacje i śledzenie nawet pojedynczych molekuł czy badanie interakcji pomiędzy białkami. Artykuł ten przybliża podstawowe techniki badań przyżyciowych z wykorzystaniem mikroskopu szerokiego pola oraz mikroskopu konfokalnego, jak również zalety i wady stosowania obu rodzajów mikroskopów.
Experiments on living organisms has always been one of the most important research in the life sciences. They allow direct observation of biological mechanisms occurring in different types of cells, microbes, or even in living animals. With the development of the various techniques for measuring fluorescence signals, using either wide field or confocal microscopy, are by now extremely well established and routine in many laboratories in the world. This resulted in development of live techniques in many areas of technology, starting with the various components (optical components, mechanical or electronic) by a conceptual approach to this type of research, ending with the creation of new fluorescent dyes which chemical properties are adjusted to the specific experiments. Thanks to the current available techniques, it is possible to observe and track even single molecules or study the interactions between proteins. This article introduces the basic techniques of live experiments using a wide field microscopy and confocal microscopy as well as the advantages and disadvantages of those two microscopes.
Źródło:
Kosmos; 2013, 62, 2; 171-180
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Deformation Mechanisms and Fracture of Ni-Based Metallic Glasses
Autorzy:
Lesz, S.
Griner, S.
Nowosielski, R.
Powiązania:
https://bibliotekanauki.pl/articles/353790.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
metals
transmission electron microscopy
scanning electron microscopy
fracture
shear bands
Opis:
The cracking of materials and fracture surface is of great practical and academic importance. Over the last few years the development of the fractography of crystalline alloys resulted in a useful tool for the prediction or failure analysis. Many attempts have been made to observe cracks using optical microscopy, X-ray topography and transmission electron microscopy (TEM). Of these techniques, the resolution of optical microscopy and X-ray topography is too poor. By contrast, the resolution of TEM is high enough for detailed information to be obtained. However, in order to apply TEM observations, a thin foil specimen must be prepared, and it is usually extremely difficult to prepare such a specimen from a pre-selected region containing a crack. In the present work, deformation mechanisms fracture surfaces of Ni-based metallic glass samples have been studied by specially designed experiments. In order to study the deformation mechanisms and fracture the Ni-based metallic glasses have been investigated in the tensile test. The structure and fracture surfaces after the decohesion process in tensile tests were observed using transmission electron microscope (TEM) and scanning electron microscope (SEM), respectively. The studies of structure were performed on thin foils. Moreover the investigated tape was subjected to a banding test. Then, the tape was straightened and the thin foil from the area of maximum strain was prepared. This thin foil sample was deformed before the TEM investigation to obtain local tears.
Źródło:
Archives of Metallurgy and Materials; 2016, 61, 2A; 791-796
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diversity of labellar micromorphological structures in selected species of Malaxidinae [Orchidales]
Autorzy:
Kowalkowska, A
Margonska, H.B.
Powiązania:
https://bibliotekanauki.pl/articles/58622.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
light microscopy
scanning electron microscopy
Orchidaceae
micromorphology
Malaxidinae
pollination
Opis:
There are no papers examining the labellar micromorphology in Malaxidinae in detail. The aim of this paper is to present the results of labellar micromorphology of our studies on Malaxidinae. The micromorphology of nine species was examined and described (calli, nectaries, OP structures, concavities). These species are divided into six groups of which the lip morphology probably represents different forms of attracting and rewarding pollinators. Group one consists of Disticholiparis gregaria and Platystyliparis aurita with characteristic globular nectary and pad-like callus. The second group with Liparis nervosa has two conelike convexities. The third one is represented by Liparis sutupensis with spread lip, the basal callus, and the epichile often eroding with age. The fourth group (Stichorkis cespitosum) has a massive hypochil with basal callus and delicate epichil. The fifth group (Microstylis ophioglossoides, Dienia ophrydis) is characterized by a lip with developed lateral lobes, three deep concavities in the middle part, an erect callus, and many raphide cells.Crepidium cf. rheedii and Crepidium dryadum from the sixth group have a three-lobed lip with entire or toothed mid-lobe, basal callus, cavity surrounded by a rim and OP structures with raphide cells. We also emphasized the different way and place of nectar secretion in the subtribe and gave some suggestions about pollination strategies.
Źródło:
Acta Societatis Botanicorum Poloniae; 2009, 78, 2; 141-150
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enhancing capabilities of Atomic Force Microscopy by tip motion harmonics analysis
Autorzy:
Babicz, S.
Smulko, J.
Zieliński, A.
Powiązania:
https://bibliotekanauki.pl/articles/199918.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
atomic force microscopy (AFM)
harmonics
van der Waals forces
Opis:
Motion of a tip used in an atomic force microscope can be described by the Lennard-Jones potential, approximated by the van der Waals force in a long-range interaction. Here we present a general framework of approximation of the tip motion by adding three terms of Taylor series what results in non-zero harmonics in an output signal. We have worked out a measurement system which allows recording of an excitation tip signal and its non-linear response. The first studies of spectrum showed that presence of the second and the third harmonics in cantilever vibrations may be observed and used as a new method of the investigated samples characterization.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2013, 61, 2; 535-539
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mikroskopowe techniki korelacji fluorescencji
Fluorescence correlation microscopy
Autorzy:
Bernaś, Tytus
Powiązania:
https://bibliotekanauki.pl/articles/1192779.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Opis:
Ruchliwość molekuł ma fundamentalne znaczenie w wielu procesach fizycznych, chemicznych oraz biologicznych. Mikroskopowe pomiary z użyciem korelacji zmian (fluktuacji) fluorescencji pozwalają wyznaczyć współczynniki dyfuzji, stałe wiązania oraz stężenia znakowanych molekuł. Niniejszy artykuł opisuje podstawy pomiarów punktowych przy użyciu korelacji fluorescencji w czasie (FCS) oraz pochodnych metod wykorzystujących przestrzenną korelację fluorescencyjnego obrazu mikroskopowego i korelację czasowo-przestrzenną.
Molecular mobility is crucial in numerous physical, chemical and biological processes. Microscopy measurements of fluorescence changes (fluctuations) can be used to estimate diffusion coefficients, binding constants and concentrations of labeled molecules. This short review describes the principles of point-wise microscopio measurements with fluorescence correlation spectroscopy. Furthermore, basics of its derivatives using spatial and spatio-temporal correlation of fluorescence microscopy data are given.
Źródło:
Kosmos; 2013, 62, 2; 205-211
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in magnetic domain structure of maraging steel studied by magnetic force microscopy
Autorzy:
Bramowicz, M.
Kulesza, S.
Mrozek, G.
Powiązania:
https://bibliotekanauki.pl/articles/298122.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
maraging steel
magnetic force microscopy
fractal analysis
Opis:
The paper presents results on whether and how the magnetic domain structure in maraging steel undergoes any change due to the aging process. It continues the works on application of correlation methods and fractal analysis into studies of magnetic properties of various steel alloys using Scanning Probe Microscopy. It is aimed at verifying the usefulness of the structure function for description of spatial changes in shape and orientation of magnetic domains, and the magnitude of magnetic stray field affected by the heat treatment. Obtained results suggest that despite vanishing magnetic tip-surface interaction, and hence vanishing magnetic stray field, magnetic domains remain nearly perfectly isotropic although randomly oriented. Similar to isotropic real surfaces, the fractal dimension of the magnetic domains turns out to be independent of the structural changes induced by the aging process, whereas the topothesy is found to vary within order of magnitude along with changes in the magnetic stray field. After all, obtained results lead to conclusion that the aging process leaves its fingerprints in the structure of the steel alloys, which can be studied using numerical analysis of images of magnetic interactions between vibrating tip and the stray field.
Źródło:
Technical Sciences / University of Warmia and Mazury in Olsztyn; 2014, 17(4); 371-379
1505-4675
2083-4527
Pojawia się w:
Technical Sciences / University of Warmia and Mazury in Olsztyn
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cast iron component failure: A metallurgical investigation
Autorzy:
Sudhakar, K. V.
Powiązania:
https://bibliotekanauki.pl/articles/382757.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cast iron
hardness test
optical microscopy
scanning electron microscopy
fractography
żeliwo
testy twardości
mikroskopia optyczna
mikroskop skaningowy
Opis:
A fractured nutcracker was examined for determining the root cause/s for premature fracture/failure. This is one of the common tools used typically for cracking hard nuts. In this study, metallurgical failure analysis techniques namely, visual inspection, optical microscopy, SEM, and hardness tests were used in investigating the broken product. From the metallurgical analysis, it was determined that the combined effect of low carbon equivalent and presence of inclusions contributed to the sudden fracture of the nut cracking tool.
Źródło:
Archives of Foundry Engineering; 2012, 12, 2s; 67-70
1897-3310
2299-2944
Pojawia się w:
Archives of Foundry Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacteria meet nanoparticles - electron microscopy insights
Autorzy:
Pajerski, Wojciech
Gołda-Cępa, Monika
Pawłyta, Mirosława
Parlińska-Wojtan, Magdalena
Brzychczy-Włoch, Monika
Kotarba, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1844929.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanoparticles
medicine
microscopy
nanocząstki
medycyna
mikroskopia
Źródło:
Engineering of Biomaterials; 2020, 23, 158 spec. iss.; 39
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie mikroskopii AFM do wizualizacji efektów prac nad otrzymaniem tlenków cyny SNO2
The SNO2 formation proces observation and visualisation using AFM
Autorzy:
Lubańska, Z.
Grudniewski, T.
Chodyka, M.
Powiązania:
https://bibliotekanauki.pl/articles/252767.pdf
Data publikacji:
2015
Wydawca:
Instytut Naukowo-Wydawniczy TTS
Tematy:
mikroskopia (AFM)
SnO2
mikroskopia skaningowa
mikroskopia atomowa
STM
AFM
AFM microscopy
scanning microscopy
atomic microscopy
Opis:
W artykule przedstawiono charakterystyki cienkich warstw SnO2 na czystym szkle. Warstwy SnO2 nanoszone były metodą magnetronowego sputteringu (nanoszenie) na podłoże szklane w różnych temperaturach. Wykorzystano do tego urządzenie Magnetron Line 440. Do badania składu i morfologii warstwy użyto mikroskopii skaningowej i atomowej (STM/AFM). Na podstawie badań stwierdzono, że powłoki wykazują dobre połączenie z materiałem podłoża, charakteryzują się zróżnicowaną chropowatością i są jednorodnie chemiczne.
Atomic force microscopy is one of the most popular method used in surface imaging. This method allows to measure the surface topography and determine the dimensions of the structures in the subatomic resolution [1]. Due to its properties, it can be applied to the measurement of conductors and semiconductor surfaces prepared in various processes. The experiment is fo-cused on SnO2 and ITO thin layers which can be used as transparent electrodes [2]. The authors are trying to illustrate the correlation between process parameters - creation of semiconductor in magnetron sputtering by different process conditions (temperature and cooling process, gas pressure and composition), surface of the sample and its other electrooptical parameters.
Źródło:
TTS Technika Transportu Szynowego; 2015, 12; 944-946, CD
1232-3829
2543-5728
Pojawia się w:
TTS Technika Transportu Szynowego
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of resonance raman microscopy to in vivo carotenoid
Autorzy:
Uragami, Chiasa
Yamashita, Eiji
Gall, Andrew
Robert, Bruno
Hashimoto, Hideki
Powiązania:
https://bibliotekanauki.pl/articles/1039772.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Raman mapping
resonance Raman microscopy
Astaxanthin
in vivo carotenoid
Opis:
The high antioxidant activity of astaxanthin has been attracted considerable attention in these days. One of the major antioxidant activities of this carotenoid is anti-photoaging. We have been focusing our attention on this particular issue. The anti-photoaging activity should be functioning in inner skin. In this study we tried to find out the fact that astaxanthin that has been swabbed on the outer surface of the skin has really passed through and reached to the inner skin. For this purpose resonance Raman microscopy was applied to the rat skin sample on which astaxanthin was swabbed on its outer surface. Astaxanthin gives rise to a unique Raman spectrum that is characteristic of its molecular structure. Therefore, we can easily identify the presence or absence of astaxanthin in the area of the rat skin that is subjected to this spectroscopic measurement. We used 532 nm laser light for probing the resonance Raman scattering of astaxanthin. Astaxanthin shows three strong Raman lines at 1508, 1145, and 993 cm-1. These three lines are ascribable to the C=C stretching, C-C stretching, and C-CH3 in-plane rocking vibrational modes, respectively. We have constructed confocal Raman microscope that has the spatial resolution of ca. 500 nm. Three-dimensional mapping of the Raman spectrum of astaxanthin has been performed in order to determine its distribution in the rat skin.
Źródło:
Acta Biochimica Polonica; 2012, 59, 1; 53-56
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
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