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Wyświetlanie 1-10 z 68
Tytuł:
Oznaczanie monenzyny w jajach
Monensin determination in eggs by thin-layer chromatography
Autorzy:
Karkocha, K.
Powiązania:
https://bibliotekanauki.pl/articles/876822.pdf
Data publikacji:
1984
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Źródło:
Roczniki Państwowego Zakładu Higieny; 1984, 35, 3
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Quantification of triazine herbicides using chloroplasts in conjunction with thin-layer chromatography
Autorzy:
Broszat, M.
Ernst, H.
Spangenberg, B.
Powiązania:
https://bibliotekanauki.pl/articles/363070.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
połączone analizy bioefektywne
derywatyzacja
HPTLC
liniowy zakres kalibracji
kwantyfikacja
TLC
herbicydy triazynowe
video densytometria
bioeffective-linked analysis
derivatization
linear calibration range
quantification
triazine herbicides
videodensitometry
Opis:
We present a video-densitometric quantification method for the triazine herbicides atraton, terbumeton, simazine, atrazine and terbutylazine. Triazine herbicides were separated on silica gel using methyl-t-butyl ether, cyclohexane (1+1, v/v) as mobile phase. The quantification was based on a bioeffective-linked analysis using chloroplast and 2,6-dichlorophenolindophenol. Within 1-2 minutes HILL-reaction inhibitor substances show blue-grey zones on a pale yellowgreen background. To increase the contrast, the moist plate can be dipped into a solution of PEG-600 (10% PEG-600 in methanol) for 2s. Measurements were carried out using a 16 bit ST-1603ME CCD camera with 1.56 megapixels (from Santa Barbara Instrument Group, Inc., Santa Barbara, USA). A white LED was used for illumination purposes. The range of linearity covers more than one magnitude using the (1/R) - 1 expression data transformation. The method can be used for herbicide screenings in environmental samples, because not spectral sensitivity but herbicide activity is measured. The separation method is cheap, fast and reliable.
Źródło:
Environmental Biotechnology; 2011, 7, 2; 47-52
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of high performance thin layer chromatography method for ophthalmological preparations containing anthocyanins fractions
Autorzy:
Skalska-Kaminska, A.
Matysik, A.
Gerkowicz, M.
Matysik, G.
Niedziela, P.
Powiązania:
https://bibliotekanauki.pl/articles/3546.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Źródło:
Journal of Pre-Clinical and Clinical Research; 2007, 01, 1
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Quantification of taxol by high-performance thin layer chromatography in Taxus wallichiana callus cultivated in vitro
Autorzy:
Mujib, A.
Tonk, D.
Gulzar, B.
Maqsood, M.
Ali, M.
Powiązania:
https://bibliotekanauki.pl/articles/2096321.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antioxidant enzymes
callus induction
stress
taxol
Taxus wallichiana
2
4-dichlorophenoxyacetic
acid
Opis:
In the present study, the induction of callus, callus biomass growth and the yield of taxol were investigated in Taxus wallichiana. This is the first report of a quantification study of taxol in in vitro grown tissues of Taxus obtained from Jammu and Kashmir provinces, India. For callus induction, three different explant types (leaf, cone and stem) were cultured in media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). The stem was most responsive to callusing, and the maximum callus induction frequency of 71.5% was noted on medium amended with 2.0 mg/l 2,4-D. The medium amended with 2,4-D and ascorbic acid (AA) showed better callus growth and the maximum biomass (0.082 g, fresh weight). The yield of taxol was quantified in the callus grown on a medium supplemented with different plant growth regulators (PGRs) by using high-performance thin layer chromatography (HPTLC). The taxol yield was maximum (1.053 μg/g dry weight) in 2,4-D-stimulated callus, followed by callus treated with NAA in which 0.896 μg/g dry weight taxol was detected. The maximum taxol yield (56.6%) was obtained in the callus grown on the medium amended with 2,4-D, and a 33.3% increased yield was noted on the NAA-supplemented medium. As a stress marker, the activities of the antioxidant enzymes superoxide dismutase (SOD) and ascorbate peroxidase (APX) and the level of proline were measured in an auxin (2,4-D and NAA)-supplemented medium. The callus grown on the 2,4-D-supplemented medium had high levels of SOD (3.91 min-1 * mg-1 protein), APX (1.61 min -1 * mg-1 protein) and proline (6.57 mg/g), thus suggesting a higher stress level; the callus grown on the NAA-supplemented medium had slightly lower levels of SOD and APX enzyme activities and proline content (3.01 min-1 * mg -1 protein, 1.04 min-1 * mg -1 protein and 5.90 mg/g, respectively). BAP had little influence on stress parameters. The present study thus indicates a good taxol yield in the callus cultured in 2,4-D, which functioned as a signalling element and a stressor. The taxol yield in response to PGRs was analysed in in vitro cultivated tissues.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 4; 337-347
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Oznaczenie monenzyny w mięsie drobiu metodą chromatografii cienkowarstwowej
Monensine determination by thin-layer chromatography
Opredelenie monenzina metodom tonkoslojjnojj chromatografii
Autorzy:
Karkocha, I.
Mazur, H.
Powiązania:
https://bibliotekanauki.pl/articles/873278.pdf
Data publikacji:
1983
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
oznaczanie
metodyka badan
monenzyna
mieso
drob
chromatografia
Źródło:
Roczniki Państwowego Zakładu Higieny; 1983, 34, 3
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie chromatografii cienkowarstwowej do oznaczania spoiw olejnych
THE USE OF THIN-LAYER CHROMATOGRAPHY IN THE DETERMINATION OF OIL BLNDERS
Autorzy:
Baczko, Krystyna
Powiązania:
https://bibliotekanauki.pl/articles/535766.pdf
Data publikacji:
1981
Wydawca:
Narodowy Instytut Dziedzictwa
Tematy:
zastosowanie chromatografii cienkowarstwowej do oznaczania spoiw olejnych
chromatografia cienkowarstwowa
identyfikacja spoiw malarskich
identyfikacja spoiw olejnych
analiza chromatograficzna cienkowarstwowa w badaniu spoiw białkowych i węglowodanowych
skład i budowa chemiczna spoiw olejnych
spoiwa olejne
analiza jakościowa olejów
Opis:
The present work made at a scientific-research laboratory of the State Enterprise for Conservation of Art was aimed at identifying oil binders by means of thin-layer chromatographic analysis. The work presented a survey of techniques employed in the determination of lipids by means of chromatographic analysis on the painted material. The procedure o f the identification of oil binders was worked out on this basis, which made it possible to implement it in conservation practices. Samples o f oils such as linseed oil (cold-pressed), poppy-seed oil (sun-dried, made by Winsor-Newton) and oil coming from the egg yolk were hydrolyzed in the solution o f KOH in methanol at room temperature. After neutralizing, the samples were extracted with chloroform. Solutions of individual oils were then put on chromatograms. Silicon gel (Kieselgel 60) was used as an adsorbent. Gel- covered glass plates (20 x 20 cm or 20 x 50 cm in size, made by Merck), were activated thermically. A number of developing systems were examined, out of which naptha oil: ethyl ether: acetic acid (80:20:1) was considered as optimum. Iodine pairs associated with sulfuric acid proved to be the best developer. The sensitivity of such a methodical procedure was defined at 10~4 g. Basing on the results of the studies, the above procedure was used for painting techniques containing oil binders: non-yellowing medium, traditional medium, oil-casein distemper, yolk distemper, distemper with arabie gum. The effect of various siccatives and inhibiting (cinnabar) and catalyzing (white lead) agents on the results o f determinations was studied in the process o f drying after prior artificial aging of samples. The method obtained makes possible to distinguish the oil technique from yolk and oil-resin techniques.
Źródło:
Ochrona Zabytków; 1981, 1-2; 86-92
0029-8247
Pojawia się w:
Ochrona Zabytków
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie metody chromatografii cienkowarstwowej do oznaczania pigmentów nieorganicznych
THE USE OF THIN LAYER CHROMATOGRAPHY IN THE DETERMINATION OF INORGANIC PIGMENTS
Autorzy:
Wróbel, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/535976.pdf
Data publikacji:
1981
Wydawca:
Narodowy Instytut Dziedzictwa
Tematy:
chromatografia cienkowarstwowa
oznaczanie pigmentów nieorganicznych
identyfikacja pigmentów
Thin-Layer Chromatography
TLC
rodzaje chromatografii cienkowarstwowej
analiza kationów
metody identyfikacji kationów
identyfikacja anionów wchodzących w skład pigmentów
metody oznaczania anionów
Opis:
It was in 1978 that a scientific and research laboratory of the State Enterprise for Monuments Conservation in Warsaw used — for the first time — thin layer chromatography to identify inorganic pigments. Basing on comprehensive literature on the analysis of inorganic ions by means of thin layer chromatography (TLC) nearly 50 methods of the determination of simple ions and their combinations were studied, partially restricting the task to methods of distribution on the most popular carriers. 15 cations entering into the composition of 28 pigments and 1 2 anions entering into the composition of 20 pigments were identidfied. To give all results of the examinations set up in more than 2 0 big tables would exceed the framework of this article. Therefore some details of the examined methods of distribution have been quoted and briefly discussed as representative. When working out methods of the distribution of individual ions or their groups standard pigments and — for comparison purposes — standard salts available in the laboratory were used. Both pigments and standard salts were prepared for spreading on plates by means of proper solvents. Most frequently it was a diluted or concentrated acid and only occasionally a flux or a diluted base. Pigment solutions were prepared and their concentration did not exceed a few per cent (usually 1 per cent solution was employed). Solutions of the examined pigments or corresponding standard salts were spread on plates covered with a layer of a carrier. As a rule Merck’s plates with silicone gel (Kieselgel 60 F22) and a carrier layer (0.25 mm thick) cut from the plates of 20 X 20 cm down to 4 X 9 cm or Merck’s plates with a cellulose carrier (Cellulose F) of 0 .1 mm in thickness and also cut down to 4 x 9 cm were used. Before spreading standards, plates with cellulose were activated in a dryer at 105° С for 10 minÿ plates with silicone gel were activated at 110° С for 1 hour. Standards were spread on plates by means of capillaries, the diameter of which was 0.5 mm. The plates were numbered and then developed in small chromatographic cells (5x3x11 cm) saturated with vapours of the developing system. After developing a chromatogram, the plates were dried in a dryer and developed by spraying with special reagents. Developed chromatograms were photographesd with a Polaroid camera. The obtained results of the studies make it possible to introduce the analysis of pigments by means of thin layer chromatography as a method supplementary to other traditional methods of determination, particularly when only a small quantity of the sample is available and it is composed of several pigments and their determination by traditional methods is impossible.
Źródło:
Ochrona Zabytków; 1981, 3-4; 188-196
0029-8247
Pojawia się w:
Ochrona Zabytków
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of Organophosphorus Compounds. 1. Application of Iodine-Azide Reaction for Detection of Thiophosphoorganic Compounds in Thin-Layer Chromatography
Autorzy:
Kotyński, Andrzej
Kudzin, Zbigniew H
Ciesielski, Witold
Powiązania:
https://bibliotekanauki.pl/articles/896207.pdf
Data publikacji:
2004
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
induced iodine-azide reaction
organophosphorus inductors
phosphorothioates
sugar phosphorothioates
thiophosphoryl nucleotides
microdetermination of thiophophoryl inductors
detection
TLC
Opis:
Wydrukowano z dostarczonych Wydawnictwu UŁ gotowych materiałów
Źródło:
Acta Universitatis Lodziensis. Folia Chimica; 2004, 13
0208-6182
Pojawia się w:
Acta Universitatis Lodziensis. Folia Chimica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Visualizing reagents for the identification and the examination of the identity of diclofenac
Autorzy:
Pyka-Pająk, Alina
Parys, Wioletta
Powiązania:
https://bibliotekanauki.pl/articles/895621.pdf
Data publikacji:
2019-12-29
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
diclofenac
thin layer chromatography
visualizing reagents
Opis:
Diclofenac sodium is often used drug with a strong anti-inflammatory, analgesic and antipyretic effect, belonging to NSAID. Diclofenac sodium was detected on plates precoated with silica gel 60F254. Nineteen new visualizing (known as dyes) namely: brilliant cresyl blue, malachite green, alkaline blue, methylene blue, methylene violet, Janus blue, bromophenol blue, brilliant green, methyl green, methyl red, bromocresol green, bromothymol green, gentian violet, crystalline violet, aniline blue, neutral red, titan yellow, thymol blue, bromothymol blue were used to detected diclofenac sodium. Rhodamine B and fuchsine were used as comparative visualizing reagents for detection of diclofenac sodium. Among all studied new visualizing reagents, brilliant-cresol blue, malachite green and titan yellow are the best to detect diclofenac sodium. Similar detection and quantitative limit values of diclofenac sodium were obtained without using visualizing reagent and after detection using the following reagents: fuchsin, alkaline blue, Janus blue, methylene blue, aniline blue and crystal violet. The coloured spots and spectrodensitograms of diclofenac sodium obtained after the use of individual visualizing reagents can be an additional parameter in studying its identity.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2019, 76, 6; 987-995
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
I Ogólnopolskie Sympozium na temat chromatografii cienkowarstwowej
Ist Polish Symposium on thin-layer chromatography
I Vsepolskijj Simpozion na temu tonkoslojjnojj khromatografii
Autorzy:
Kucharczyk, W.
Powiązania:
https://bibliotekanauki.pl/articles/838733.pdf
Data publikacji:
1967
Wydawca:
Polskie Towarzystwo Parazytologiczne
Źródło:
Annals of Parasitology; 1967, 13, 2-3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
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