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Tytuł:
Multi-method approach to identification of species of Trochulus (Gastropoda: Hygromiidae)
Autorzy:
Prockow, M.
Strzala, T.
Kuznik-Kowalska, E.
Prockow, J.
Mackiewicz, P.
Powiązania:
https://bibliotekanauki.pl/articles/83710.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
Tematy:
species identification
identification method
identification
Trochulus
snail
species number
morphology
anatomy
Gastropoda
Hygromiidae
Źródło:
Folia Malacologica; 2016, 24, 1
1506-7629
Pojawia się w:
Folia Malacologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of Cercospora species in southwestern Iran
Autorzy:
Behrooz, Seyed Yousef
Salari, Mohamad
Pirnia, Mahdi
Kazem Sabbagh, Seyed
Powiązania:
https://bibliotekanauki.pl/articles/961647.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cercosporoid fungi
leaf spot
plant diseases
taxonomy
Opis:
Cercospora species are associated with leaf spot symptoms on various host plants. In this research, nine species of the genus on some medicinal and economic crops were found in different locations in Kohgiluyeh and Boyerahmad Provinces (southwestern Iran) and examined according to morphological characteristics of stromata, conidiophores, conidiogenous cells and conidia. Results showed that Cercospora species on Cichorium intybus (C. cichorii) and Nasturtium officinale (C. nasturtii) are new for the mycobiota of Iran. However, characteristics of Cercospora on Plantago lanceolata are very similar to the description of C. plantaginis, but morphologically indistinguishable from C. apii s. lat. Other species have already been reported from other parts of Iran, but are new in southwestern Iran. Furthermore, Rumex crispus and Trifolium resupinatum are new hosts for C. apii and C. zebrina in Iran, respectively.
Źródło:
Journal of Plant Protection Research; 2017, 57, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia
Autorzy:
Sawicki, J.
Szczecinska, M.
Powiązania:
https://bibliotekanauki.pl/articles/57748.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Acutifolia
random amplified polymorphic DNA
Sphagnum
genetic similarity
molecular identification
molecular marker
polymerase chain reaction
genetic relationship
species identification
peat moss
chloroplast
nuclear genome
Opis:
RAPDs, ISJs, ISSRs, ITS and katGs were applied to determine genetic relationships between common Sphagnum species of the section Acutifolia. Twenty populations were genotyped using ten ISJ primers, 12 pairs of katG primers, 10 ISSR and 10 RAPD primers, and a restriction analysis of ITS1 and ITS2. ISSR and katG markers revealed the greatest number of species-specific bands. An analysis of ITS1 and ITS2 regions with restriction enzymes also proved to be a highly effective tool for species identification.
Źródło:
Acta Societatis Botanicorum Poloniae; 2011, 80, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie metod molekularnych w identyfikacji gatunku, wieku i płci owadów użytecznych w entomologii sądowej
Use of molecular methods in the identification of the species, age and sex of insects useful in forensic entomology
Autorzy:
Stojak, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1373968.pdf
Data publikacji:
2014
Wydawca:
Centralne Laboratorium Kryminalistyczne Policji
Tematy:
entomologia sądowa
barkoding DNA
mtDNA
metody molekularne
identyfikacja gatunkowa
forensic entomology
DNA barcoding
molecular methods
identification of species
Opis:
Entomologia sądowa wykorzystuje owady do ustalania czasu i przyczyny śmierci, a nawet miejsca, w którym nastąpiła. W tym celu stosowane są dwie metody. Metoda rozwojowa opiera się na wzorcach rozwoju larw w określonych warunkach temperaturowo-środowiskowych. Metoda sukcesyjna analizuje występujące w różnych środowiskach wzorce pojawiania się poszczególnych taksonów na zwłokach. W obu tych metodach najistotniejszą kwestią jest poprawna identyfikacja gatunków. W poniższym artykule zaprezentowane zostały molekularne metody identyfikacji, takie jak barkoding DNA czy analiza krzywych denaturacji DNA o wysokiej rozdzielczości (DNA-HRM-PCR).
Forensic entomology uses insects to determine the time, cause and place of death. To this end, two entomological methods are used. The development-based method uses the patterns of insect larvae development under the specific thermal and environmental conditions. The succession-based method analyzes the sequence of insect succession on the body in various environmental conditions. The proper insect species identification is essential in both methods. In this article, the molecular methods of species, age and sex identification are presented such as DNA barcoding or DNA-HRM-PCR.
Źródło:
Problemy Kryminalistyki; 2014, 286; 22-26
0552-2153
Pojawia się w:
Problemy Kryminalistyki
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molekularna identyfikacja gatunków z rodzaju Abies na podstawie polimorfizmu DNA mitochondrialnego
Molecular identification of species from Abies genus based on the mitochondrial DNA polymorphism
Autorzy:
Pawlaczyk, E.M.
Staniak, J.
Maliński, T.
Bobowicz, M.A.
Powiązania:
https://bibliotekanauki.pl/articles/989708.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
drzewa lesne
genetyka roslin
jodla
Abies
gatunki roslin
identyfikacja
haplotypy
DNA mitochondrialny
polimorfizm DNA
abies species
haplotype
capillary electrophoresis
mitochondrial marker
Opis:
The plant material was collected on 34 individuals growing in the Dendrological Garden of Poznań University of Life Sciences (52°25'32,95" N 16°53'39,83" E) and Botanical Garden of Adam Mickiewicz University in Poznań (52°25'11,70" N 16°52'55,07" E). The species for this study originated from Europe, Asia Minor, central and eastern Asia and North America and included: Abies alba, Abies cephalonica, Abies cilicica, Abies equi−trojani, Abies sibirica, Abies koreana, Abies pinsapo, Abies ×insignis, Abies bornmulleriana, Abies homolepsis, Abies holophylla, Abies grandis, Abies concolor, Abies concolor var. violacea, Abies concolor var. lowiana, Abies nordmanniana, Abies ×arnoldiana, Abies nephrolepis and Abies balsamea. The aim of this study was to define the species haplotypes (the length of allele) on the basis of nad5−4 mitochondrial DNA marker detected by capillary electrophoresis. This marker has been suggested as an easy−to−use tool to distinguish species of the Abies genus and it could be species−specific. Seven different haplotypes were identified. The first one appears in the species from Europe, Asia and North America. The second one was detected in firs from Europe and Asia Minor. A. cephalonica and A. sibirica were identified by the third haplotype, which occurs also in A. alba from the Balkan region. The fourth haplotype is characteristic for species from Asia and North America. The fifth and sixth haplotypes were identified in A. pinsapo and A. numidica. The seventh haplotype was detected only in A. holophylla. Applied marker is a very useful for verification of fir species especially allopatric species, less for parapatric ones. This marker is more helpful to exclude the species than to precisely identify them.
Źródło:
Sylwan; 2015, 159, 08; 675-683
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of Ephedra species by phylogenetic analyses using matK and ITS1 sequences
Autorzy:
Zhao, Y.S.
Xie, L.X.
Mao, F.Y.
Cao, Z.
Wang, W.P.
Zhao, Q.P.
Zhang, X.H.
Powiązania:
https://bibliotekanauki.pl/articles/56673.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Botaniczne
Opis:
In this study, the species identifications of seven Ephedra plants, including three medicinal plants from the Pharmacopoeia of the People’s Republic of China, were conducted using phylogenetic analyses, and the method’s validity was verified. The phylogenetic trees constructed from the maturase-coding gene (matK) and internal transcribed spacer 1 (ITS1) sequences showed that the former could be used for identifying five Ephedra plants, Ephedra intermedia, E. equisetina, E. antisyphilitica, E. major, and E. aphylla, but it had less power to discriminate E. sinica and E. przewalskii, while the latter could distinguish five Ephedra plants, E. przewalskii, E. equisetina, E. antisyphilitica, E. major, and E. aphylla, but it had less power to discriminate E. sinica and E. intermedia. However, when the two genes were combined, the seven species could be completely distinguished from each other, especially the medicinal plants from the others, which is significant in developing their pharmaceutical uses and in performing quality control assessments of herbal medicines. The method presented here could be applied to the analysis of processed Ephedra plants and to the identification of the botanical origins of crude drugs. Additionally, we discovered that E. equisetina and E. major were probably closely related to each other, and that E. sinica, E. intermedia, and E. przewalskii also had a close genetic relationship.
Źródło:
Acta Societatis Botanicorum Poloniae; 2016, 85, 2
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection and molecular identification of Cryptosporidium species in laboratory rats (Rattus norvegicus) in Ibadan, Nigeria
Autorzy:
Ayinmode, Adekunle Bamidele
Ogbonna, Nkeiruka Fortunate
Widmer, Giovanni
Powiązania:
https://bibliotekanauki.pl/articles/972090.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
laboratory rat
microscopy
pcr
sequencing
Cryptosporidium species
Opis:
To study the occurrence of Cryptosporidium infection in laboratory rats (Rattus norvegicus) raised for experimental usage, 134 faecal samples were obtained from two rearing houses in Ibadan and examined for the presence of Cryptosporidium oocyst using the modified acid fast staining technique. Cryptosporidium species in 2 samples positive for microscopy were further characterized by a nested polymerase chain reaction (PCR) amplifying the 18S rRNA gene. Two of 134 samples were positive for the Cryptosporidium oocysts. Sequencing of the small-subunit rRNA amplicons identified the species in the two PCR positive samples as Cryptosporidium andersoni and Cryptosporidium rat genotype. These findings showed that laboratory rat is a potential reservoir for diverse Cryptosporidium species and suggests that laboratory rats should be screened for Cryptosporidium infection prior to experiments, especially where pathogen free animals are not available. This the first report to identify Cryptosporidium species infecting laboratory rats in Nigeria.
Źródło:
Annals of Parasitology; 2017, 63, 2; 105-109
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of the suitability of mitochondrial DNA for species identification of microtraces and forensic traces
Autorzy:
Natonek-Wiśniewska, Małgorzata
Krzyścin, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1038564.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
biological traces
forensic DNA analysis
species identification of forensic DNA
species identification of biological traces
mtDNA
Opis:
The objective of the study was to demonstrate how mitochondrial DNA (mtDNA) can be used to determine the species origin of animal microtraces. The study included pieces of cat and dog hair without the root, a fragment of cooked chicken bone (0.1g), three goose down samples (0.028 g), a pork swab, a pork scratching (5×5×5 mm), and pork lard (0.22 g). DNA was isolated from all of these samples using the method appropriate for the particular source material. The extracts had DNA concentration exceeding 5.4 ng/µl with A260/280 purity range of 1.14-1.88. Next, the samples were subjected to PCR and real-time PCR with species-specific primers and primers complementary to mitochondrial DNA (mtDNA). Control reactions based on the amplification of eukaryotic-specific fragment (18S rRNA) were additionally performed. PCR and real-time PCR products for detection of species-specific mtDNA were obtained for all templates, whereas during the detection of eukaryote DNA no product was obtained for dog and cat hair only. The poor quality of the obtained DNA did not prevent the analysis. The results showed that mitochondrial DNA is suitable for identification of small or highly processed samples, in which genomic DNA often cannot be analyzed.
Źródło:
Acta Biochimica Polonica; 2017, 64, 4; 705-708
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of three species commonly known as“daqingye” by internal leaf anatomy and high-performance liquid chromatography analyses
Autorzy:
Zhang, Y.
Zhu, Y.
Chen, J.
Xia, C.
Deng, J.
Li, H.
Li, Y.
Li, J.
Liu, P.
Powiązania:
https://bibliotekanauki.pl/articles/59301.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Botaniczne
Opis:
The macroscopic and microscopic morphologies and indigo and indirubin concentration of the traditional Chinese medicine herbs Isatis indigotica Fort., Polygonum tinctorium Ait., and Baphicacanthus cusia (Nees) Bremek, all commonly known as “daqingye”, were determined and compared. The morphological analyses indicated that I. indigotica has leaves with winged petioles and no glandular hairs or crystals, P. tinctorium has leaves with membranous ocrea and clusters of calcium oxalate, and B. cusia has palisade cells in the mesophyll running over the main vein and single cells containing calcium carbonate crystals. Indigo and indirubin are chemical constituents that have been previously isolated from daqingye and were selected in this study as identification markers for high-performance liquid chromatography analysis due to their pharmacological activities. The chromatographic results showed that indigo and indirubin concentration varied significantly among the three species: high concentration of both indigo and indirubin were observed in I. indigotica, the highest concentration among the three daqingye plants was found in P. tinctorium but with low levels of indirubin, and the concentration of indigo and indirubin was quite low in B. cusia. In summary, three different species commonly known as daqingye were accurately distinguished by morphological observation, internal leaf anatomy analysis, and chromatographic analysis.
Źródło:
Acta Societatis Botanicorum Poloniae; 2018, 87, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification and binomial computerization of plant species
Autorzy:
Karthik, K.
Pugalenthi, M.
Sharavanan, P.S.
Powiązania:
https://bibliotekanauki.pl/articles/11186.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
identification
binomial computerization
plant species
new technology
computerization process
Opis:
The binomial computerization method is comparison of certain similarities and differences of plants identification system. The ultimate purpose of the plants identification process is method of using digital tools of database and creating new version of plants identification for constructing the digital keys. The present study of plant identification system is based on computerization processes. The plants species characters are constructing basis on taxonomical literature and followed by suitable classification system and binomial rules.
Źródło:
International Letters of Natural Sciences; 2014, 14
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
ROSMETER: a bioinformatic tool for the identification of reactive oxygen species (ROS) transcriptomic imprints
Autorzy:
Rosenwasser, S.
Sela, N.
Fluhr, R.
Friedman, H.
Powiązania:
https://bibliotekanauki.pl/articles/80882.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
reactive oxygen species
subcellular localization
leaf
transcriptome
abiotic stress
biotic stress
molecular mechanism
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular identification of pathogenic Fusarium species, the causal agents of tomato wilt in western Iran
Autorzy:
Chehri, K.
Powiązania:
https://bibliotekanauki.pl/articles/66185.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular identification
pathogen
Fusarium
causal agent
tomato
Lycopersicon esculentum
wilt pathogen
pathogenicity
phylogenetic analysis
Iran
Opis:
Fusarium species are causal agents of fungal diseases occurring frequently in numerous agriculturally important plants, including potato, garlic and are one of the common pathogens of tomato, causing root rot in the west part of Iran. Therefore, the objectives of this study were to isolate and identify disease-causing Fusarium species from infected tomatoes based on the morphological and molecular characteristics. Twenty-five isolates of Fusarium were obtained from infected root of tomato plants collected from the fields in different regions of western Iran. Based on morphological features, the strains were classified into four following Fusarium species: F. oxysporum, F. redolens, F. proliferatum and F. verticillioides. The phylogenetic trees based on tef1 and tub2 dataset clearly distinguished closely related species. All of the isolates were evaluated for their pathogenicity on healthy tomato seedlings in the greenhouse. This is the first report on molecular identification of Fusarium species isolated from tomato plants cultivated in Iran.
Źródło:
Journal of Plant Protection Research; 2016, 56, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphological and molecular identification of cyst nematode species (Heterodera spp.) in Algerian cereal fields
Autorzy:
Smaha, D.
Mokrini, F.
Imren, M.
Mokabli, A.
Dababat, A.A.
Powiązania:
https://bibliotekanauki.pl/articles/2084684.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cereals
Heterodera spp.
cyst nematode
ITS-rDNA
wheat
Opis:
Cereal cyst nematodes (Heterodera spp.) are distributed globally and cause severe production losses of small grain cereals. To investigate the occurrence of cereal cyst nematodes in wheat-growing areas of Algeria, a survey was conducted and 27 cereal cyst nematode populations were collected. The populations were initially identified based on their morphological and morphometric characters, followed by molecular methods using speciesspecific primers, complemented by ITS-rDNA sequences. The morphological and morphometric features of second-stage juveniles (J2s) and cysts supported the presence of three Heterodera species: H. avenae, H. filipjevi and H. hordecalis. All morphological values of these distinct populations were very similar to those previously described for these species. Using species-specific primers for H. avenae and H. filipjevi, the specific bands of 109 bp and 646 bp confirmed the morphological identification of both species, respectively. In addition, the internal transcribed spacer (ITS) regions were sequenced to study the diversity of the 27 populations. These sequences were compared with those of Heterodera species available in the GenBank database (www.ncbi.nlm.nih.gov) and re-confirmed the identity of the species. Nineteen sequences of ITS-rDNA were similar (99–100%) to the sequences of H. avenae published in the GenBank, six sequences were similar (99–100%) to H. hordecalis, and two were similar (98–99%) to H. filipjevi. The results of this study are of great value to breeding programs and extension services, where they will contribute to the design of control measures to keep damaging nematodes in check.
Źródło:
Journal of Plant Protection Research; 2019, 59, 3; 400-411
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification and diversity of Fusarium species isolated from tomato fruits
Autorzy:
Murad, N.B.A.
Kusai, N.A.
Zainudin, N.A.I.M.
Powiązania:
https://bibliotekanauki.pl/articles/66221.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
identification
diversity
Fusarium oxysporum
Gibberella fujikuroi
isolate
tomato fruit
Opis:
Fruit rot of tomato is a serious disease caused by Fusarium species. Sampling was conducted throughout Selangor, Malaysia and fungal species identification was conducted based on morphological and gene encoding translation elongation factor 1-α (tef1-α) sequence analysis. Five species of Fusarium were discovered namely F. oxysporum (including F. oxysporum f. sp. lycopersici), F. solani, F. equiseti, F. proliferatum and F. verticillioides. Our results provide additional information regarding the diversity of Fusarium species associated with fruit rot disease of tomato.
Źródło:
Journal of Plant Protection Research; 2016, 56, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Screening and identification of novel cellulolytic Trichoderma species from Egyptian habitats
Autorzy:
Hewedy, O.A.
El-Zanaty, A.M.
Fahmi, A.I.
Powiązania:
https://bibliotekanauki.pl/articles/2097005.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Trichoderma
cellulase activity
biodegradation
ITS
tef1
Opis:
The present study aimed to isolate indigenous Trichoderma fungi possessing cellulolytic activities from different geographical locations in Egypt. Such strains can be used to convert crop residues to bioproducts, which presently is one of the most important issues in developing countries. The isolates were identified using molecular approaches and morphological characteristics. Favorable growth conditions for Trichoderma isolates (media & temperature) were assessed in vitro. The growth rate of Trichoderma isolates was assessed on two media, namely Potato Dextrose Agar (PDA) and Synthetic Nutrient Agar (SNA), and morphological parameters, i.e., shape, pigment, and edge were observed. A comparison of sequences encoding for internal transcribed spacer (ITS) and translation elongation factor 1-α (tef1 ) was conducted to determine the species. Cellulase assay was performed using different cellulose substrates (Swollen, CMC (low viscosity carboxymethylcellulose), and Avicel). Among the isolated fungi, Trichoderma viride EGY-T27 had the highest endoglucanase activity, while Trichoderma harzianum EGY-T30 showed the highest exoglucanase activity. A total of 27 strains of Trichoderma were identified using ITS, and the tef1 gene was sequenced for 23 strains. The tested strains were identified with acceptable quality based on ITS sequences, which ranged from 98 to 100%, except for strain MH908499 (85%). This research constitutes the most comprehensive study on screening for fungal strains with the highest biodegradable potential isolated from various locations in Egypt.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 2; 117-133
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The uselessness of cytochrome oxydase gene analysis for genetic identification of selected Gobiidae species from some European countries
Autorzy:
Kiełpińska, Jolanta
Czerniejewski, Przemysław
Adamkowska, Natalia
Uzunova, Eliza
Powiązania:
https://bibliotekanauki.pl/articles/2203561.pdf
Data publikacji:
2022
Wydawca:
Instytut Technologiczno-Przyrodniczy
Tematy:
cytochrome oxidase subunit 1 gene
COI gene
Gobiidae
invasive species
native species
mt DNA
Opis:
The family Gobiidae is the largest teleost family, consisting of more than 2,000 species in more than 200 genera. They have adapted to a wide range of habitats, inhabiting mostly marine and brackish waters. Recently invasive Ponto-Caspian gobies have caused significant changes to the structure of fish assemblage throughout the European waters. The aim of the study was to verify the possibility of identifying three species of the genus Gobiidae based on the analysis of the mitochondrial region of the cytochrome oxidase subunit 1 (COI) gene. 339 samples originated from seven countries and 17 different sites were investigated. Qualitative and quantitative assessment of the extracted DNA was conducted by measuring light absorbance and electrophoretic separation. Approximately 650-bp (base pairs) fragments were amplified from the 5' region of the mitochondrial COI with the PCR (polymerase chain reaction) reaction. Sequences of 652 bp were obtained and compared with reference sequences in the National Center for Biotechnology Information (NCBI). Based on the sequence analysis, we determined that in the analysed geographical regions, the goby preliminary identified morphologically as the round goby was represented by three species: round goby (Neogobius melanostomus), tubenose goby (Proterorhinus marmoratus), and monkey goby (Neogobius fluviatilis). The latter two are newly reported for their new habitats. Genetic characterisation of such populations offers the opportunity to assess their genetic stability, which provides agencies managing the aquatic environment in EU countries with a tool for revising the legislation, including regulations relevant for updating the status of native and invasive species in European waters.
Źródło:
Journal of Water and Land Development; 2022, Special Issue; 8--14
1429-7426
2083-4535
Pojawia się w:
Journal of Water and Land Development
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of bacterial species in milk by MALDI-TOF and assessment of some oxidant-antioxidant parameters in blood and milk from cows with different health status of the udder
Autorzy:
Ozbey, G.
Cambay, Z.
Yilmaz, S.
Aytekin, O.
Zigo, F.
Ozçelik, M.
Otlu, B.
Powiązania:
https://bibliotekanauki.pl/articles/16539172.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
dairy cows
CMT
mastitis
malondialdehyde
glutathione
MALDI-TOF
Opis:
This study aimed to identify bacterial pathogens in milk samples from dairy cows with subclinical and clinical mastitis as well as to assess the concentrations of oxidant-antioxidant parameters [malondialdehyde (MDA), reduced glutathione (GSH), and total GSH levels] in both blood and milk samples. From a total of 200 dairy cows in 8 farms, 800 quarter milk samples obtained from each udder were tested in the laboratory for the presence of udder pathogens. Cultivated bacteria causing intramammary infection from milk samples were identified by Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF). In addition, from tested animals 60 cows were selected includıng 20 healthy cows that were CMT negative, 20 cows with subclinical mastitis (SM), and 20 cows with clinical mastitis (CM) for detection of MDA, GSH, and total GSH levels in blood and milk samples. Three hundred and eighty (47.5%; 380/800), 300 (37.5%; 300/800), and 120 (15%; 120/800) of milk samples, respectively were CMT positive or SM and CM, and those positives were cows from different farms. We observed that 87.4% (332/380), 25.3% (76/300), and 34.2% (41/120) of cows with CMT positive, CMT negative, and CM had bacterial growth. The most predominantly identified bacteria were Staphylococcus chromogenes (18.7%) obtained mainly from SM and Staphylococcus aureus (16.7%) as the most frequent cause of CM. According to our results, dairy cows with CM had the highest MDA levels, the lowest GSH, and total GSH levels in both blood and milk samples however, high MDA levels and low GSH levels in milk samples with SM were observed. Based on our results, lipid oxidant MDA and antioxidant GSH could be excellent biomarkers of cow’s milk for developing inflammation of the mammary gland. In addition, there was no link between nutrition and MDA and GSH levels.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 2; 269-277
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Application of PCR Reaction for Identification of MHB Bacteria Species
Zastosowanie reakcji PCR do identyfikacji gatunkowej bakterii MHB
Autorzy:
Ząbkiewicz, A.
Myga-Nowak, M.
Bandurska, K.
Paczyńska, J.
Szybecka, A.
Krupa, P.
Powiązania:
https://bibliotekanauki.pl/articles/205412.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mycorrhiza
helper bacteria
MHB
16S rRNA gene
mikoryza
bakterie wspomagające
gen 16sRNA
Opis:
This study characterizes mycorrhiza helper bacteria (MHB) from selected unpolluted locations as well as subjected to industrial emissions. To determine the species of bacteria isolated from the roots of ectomycorrhizal pine and birch, a method based on the sequence analysis of a 16S rRNA gene was used. The isolated bacteria were initially characterized by available biochemical methods and phenotypic observation. On the selected bacteria representatives isolation of DNA was performed, on which the PCR reaction was carried out. In this way amplified samples were automatically sequenced and the obtained results were compared to public databases. Among the isolated bacteria Pseudomonas fluorescens SBW25 and Burkholderia xenovorans LB400 species were dominant.
W pracy scharakteryzowano bakterie wspomagające mikoryzę pochodzące z wybranych terenów ekologicznie czystych oraz z terenów poddanych emisji przemysłowej. Przedstawiono wykorzystanie metody opartej na analizie sekwencji genu 16S rRNA do określenia przynależności gatunkowej bakterii wyizolowanych z korzeni ektomikoryzowych sosny i brzozy. Wyizolowane bakterie zostały wstępnie scharakteryzowane przy pomocy dostępnych metod biochemicznych i obserwacji fenotypowej. Dla wybranych przedstawicieli dokonano izolacji DNA, względem którego przeprowadzono reakcję PCR. Powielone w ten sposób próbki automatycznie zsekwencjonowano, a uzyskane sekwencje porównywano w ogólnodostępnych bazach danych. Wśród wyizolowanych bakterii dominowały gatunki Pseudomonas fluorescens SBW25 oraz Burkholderia xenovorans LB400.
Źródło:
Archives of Environmental Protection; 2014, 40, 2; 115-122
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Określenie składu gatunkowego lasów Góry Chojnik (Karkonoski Park Narodowy) z wykorzystaniem lotniczych danych hiperspektralnych APEX
Identification of tree species in Mt Chojnik (Karkonoski National Park) forest using airborne hyperspectal APEX data
Autorzy:
Raczko, E.
Zagajewski, B.
Ochtyra, A.
Jarocińska, A.
Marcinkowska-Ochtyra, A.
Dobrowolski, M.
Powiązania:
https://bibliotekanauki.pl/articles/989774.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
Karkonoski Park Narodowy
gory
Chojnik
lasy
sklad gatunkowy
metody badan
teledetekcja
pomiary hiperspektralne
skaner APEX
svm classification
apex hyperspectral data
species structure
Opis:
We used hyperspectral data from APEX scanner (288 spectral bands in 380−2500 nm spectral range; 3,5 m spatial resolution) to classify five tree species occurring in the area of Mt. Chojnik in the Karkonoski National Park (south−western Poland). Data used to delimit learning and verification polygons were acquired during field research in August 2013, when ground truth polygons were acquired using device equipped with GPS receiver. Raw APEX data went through radiometric and geometric correction at VITO office. To reduce processing time, 40 most informative bands were selected using information content analysis. The Support Vector Machines (SVM) algorithm was used for classification of the following tree species: Fagus sylvatica L., Betula pendula Roth, Pinus sylvestris L., Picea alba L. Karst and Larix decidua Mill. Final classification had 78.66% overall accuracy with Kappa coefficient equal to 0.71. The best classified species included beech (87.09%) and pine (83.96%), while the worst results were obtained for larch (60.29%). Low accuracy for larch could be caused by the fact that most of larch trees in the research area grow in small patches, which made it hard to specify large enough sample of training data. All classified tree species had producer's accuracy of at least 60%, with the highest value reaching 87%. User's accuracies were from 53% for pine to 85% for beech. It is possible to classify tree species using hyperspectral data with moderate to high accuracy even if the data used lacked atmospheric correction. Further work will focus on improving the classification accuracy and use of neural networks based classification methods. Results from this paper will serve as basis for tree species map of the Karkonoski National Park.
Źródło:
Sylwan; 2015, 159, 07; 593-599
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Species identification and phylogenetic analysis of six species of Diplostomum Nord. [Digenea]
Autorzy:
Niewiadomska, K.
Borsuk, P.
Laskowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/837536.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Diplostomum mergi
Diplostomum paracaudum
Diplostomum baeri
Diplostomum
metacercaria
Diplostomum parviventosum
Diplostomum spathaceum
Diplostomum pseudospathaceum
cercaria
Digenea
identification
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A digitized database of bark morphology for identification of common tree species and literature study of bark phytochemicals and therapeutic usage
Autorzy:
Biswas, Shilpa
Gupta, Kaushik
Talapatra, Soumendra Nath
Powiązania:
https://bibliotekanauki.pl/articles/1190149.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Bark morphology
Bark images database
Phytochemicals in bark
Common tree species
Terrestrial ecosystem
Opis:
Bark is used for several therapeutic purposes by traditional knowledge from ancient time. The present study was attempted to digitize the bark morphology by image based database for common tree species and compilation of recent research works by literature survey to know potent phyto- constituents present in the bark of tree species and their ability in disease prevention. In conclusion, present study of digital database on bark morphology may help easy detection of common tree species in biodiversity research work. In the present study, also the compilation of bark phytochemicals and their therapeutic efficiencies may also serve a databank as ready references in future pharmacological researches.
Źródło:
World Scientific News; 2016, 42; 143-155
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Techniki molekularne stosowane w identyfikacji gatunkow Toxocara
Molecular techniques applied in species identification of Toxocara
Autorzy:
Fogt, R
Powiązania:
https://bibliotekanauki.pl/articles/840204.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
identyfikacja gatunkowa
Toxocara canis
Toxocara cati
polimorfizm molekularny
lancuchowa reakcja polimerazy
nicienie
pasozyty
losowa amplifikacja DNA
Toxocara
Opis:
Toxocarosis is still an important and actual problem in human medicine. It can manifest as visceral (VLM), ocular (OLM) or covert (CT) larva migrans syndroms. Complicated life cycle of Toxocara, lack of easy and practical methods of species differentiation of the adult nematode and embarrassing in recognition of the infection in definitive hosts create difficulties in fighting with the infection. Although studies on human toxocarosis have been continued for over 50 years there is no conclusive answer, which of species - T. canis or T. cati constitutes a greater risk of transmission of the nematode to man. Neither blood serological examinations nor microscopic observations of the morphological features of the nematode give the satisfied answer on the question. Since the 90-ths molecular methods were developed for species identification and became useful tools being widely applied in parasitological diagnosis. This paper cover the survey of methods of DNA analyses used for identification of Toxocara species. The review may be helpful for researchers focused on Toxocara and toxocarosis as well as on detection of new species. The following techniques are described: PCR (Polymerase Chain Reaction), RFLP (Restriction Fragment Length Polymorphism), RAPD (Random Amplified Polymorphic DNA) and SSCP (Single Strand Conformation Polymorphism).
Źródło:
Annals of Parasitology; 2006, 52, 1; 31-35
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of three genomic species of Borrelia burgdorferi sensu lato in lysates and isolates originated from Ixodes ricinus ticks. Preliminary results
Autorzy:
Stanczak, J.
Kur, J.
Racewicz, M.
Kubica-Biernat, B.
Kruminis-Lozowska, W.
Dabrowski, J.
Powiązania:
https://bibliotekanauki.pl/articles/839978.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Polska
Lyme disease
Borrelia burgdorferi
Ixodes ricinus
tick
identification
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Techniki molekularne stosowane w identyfikacji gatunków Toxocara
Molecular techniques applied in species identification of Toxocara
Autorzy:
Fogt, R.
Powiązania:
https://bibliotekanauki.pl/articles/2144086.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
identyfikacja gatunkowa
Toxocara canis
Toxocara cati
polimorfizm molekularny
lancuchowa reakcja polimerazy
nicienie
pasozyty
losowa amplifikacja DNA
Toxocara
Opis:
Toxocarosis is still an important and actual problem in human medicine. It can manifest as visceral (VLM), ocular (OLM) or covert (CT) larva migrans syndroms. Complicated life cycle of Toxocara, lack of easy and practical methods of species differentiation of the adult nematode and embarrassing in recognition of the infection in definitive hosts create difficulties in fighting with the infection. Although studies on human toxocarosis have been continued for over 50 years there is no conclusive answer, which of species - T. canis or T. cati constitutes a greater risk of transmission of the nematode to man. Neither blood serological examinations nor microscopic observations of the morphological features of the nematode give the satisfied answer on the question. Since the 90-ths molecular methods were developed for species identification and became useful tools being widely applied in parasitological diagnosis. This paper cover the survey of methods of DNA analyses used for identification of Toxocara species. The review may be helpful for researchers focused on Toxocara and toxocarosis as well as on detection of new species. The following techniques are described: PCR (Polymerase Chain Reaction), RFLP (Restriction Fragment Length Polymorphism), RAPD (Random Amplified Polymorphic DNA) and SSCP (Single Strand Conformation Polymorphism).
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 1; 31-35
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
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