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Wyświetlanie 1-10 z 72
Tytuł:
Multi-method approach to identification of species of Trochulus (Gastropoda: Hygromiidae)
Autorzy:
Prockow, M.
Strzala, T.
Kuznik-Kowalska, E.
Prockow, J.
Mackiewicz, P.
Powiązania:
https://bibliotekanauki.pl/articles/83710.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
Tematy:
species identification
identification method
identification
Trochulus
snail
species number
morphology
anatomy
Gastropoda
Hygromiidae
Źródło:
Folia Malacologica; 2016, 24, 1
1506-7629
Pojawia się w:
Folia Malacologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of Cercospora species in southwestern Iran
Autorzy:
Behrooz, Seyed Yousef
Salari, Mohamad
Pirnia, Mahdi
Kazem Sabbagh, Seyed
Powiązania:
https://bibliotekanauki.pl/articles/961647.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cercosporoid fungi
leaf spot
plant diseases
taxonomy
Opis:
Cercospora species are associated with leaf spot symptoms on various host plants. In this research, nine species of the genus on some medicinal and economic crops were found in different locations in Kohgiluyeh and Boyerahmad Provinces (southwestern Iran) and examined according to morphological characteristics of stromata, conidiophores, conidiogenous cells and conidia. Results showed that Cercospora species on Cichorium intybus (C. cichorii) and Nasturtium officinale (C. nasturtii) are new for the mycobiota of Iran. However, characteristics of Cercospora on Plantago lanceolata are very similar to the description of C. plantaginis, but morphologically indistinguishable from C. apii s. lat. Other species have already been reported from other parts of Iran, but are new in southwestern Iran. Furthermore, Rumex crispus and Trifolium resupinatum are new hosts for C. apii and C. zebrina in Iran, respectively.
Źródło:
Journal of Plant Protection Research; 2017, 57, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia
Autorzy:
Sawicki, J.
Szczecinska, M.
Powiązania:
https://bibliotekanauki.pl/articles/57748.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Acutifolia
random amplified polymorphic DNA
Sphagnum
genetic similarity
molecular identification
molecular marker
polymerase chain reaction
genetic relationship
species identification
peat moss
chloroplast
nuclear genome
Opis:
RAPDs, ISJs, ISSRs, ITS and katGs were applied to determine genetic relationships between common Sphagnum species of the section Acutifolia. Twenty populations were genotyped using ten ISJ primers, 12 pairs of katG primers, 10 ISSR and 10 RAPD primers, and a restriction analysis of ITS1 and ITS2. ISSR and katG markers revealed the greatest number of species-specific bands. An analysis of ITS1 and ITS2 regions with restriction enzymes also proved to be a highly effective tool for species identification.
Źródło:
Acta Societatis Botanicorum Poloniae; 2011, 80, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie metod molekularnych w identyfikacji gatunku, wieku i płci owadów użytecznych w entomologii sądowej
Use of molecular methods in the identification of the species, age and sex of insects useful in forensic entomology
Autorzy:
Stojak, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1373968.pdf
Data publikacji:
2014
Wydawca:
Centralne Laboratorium Kryminalistyczne Policji
Tematy:
entomologia sądowa
barkoding DNA
mtDNA
metody molekularne
identyfikacja gatunkowa
forensic entomology
DNA barcoding
molecular methods
identification of species
Opis:
Entomologia sądowa wykorzystuje owady do ustalania czasu i przyczyny śmierci, a nawet miejsca, w którym nastąpiła. W tym celu stosowane są dwie metody. Metoda rozwojowa opiera się na wzorcach rozwoju larw w określonych warunkach temperaturowo-środowiskowych. Metoda sukcesyjna analizuje występujące w różnych środowiskach wzorce pojawiania się poszczególnych taksonów na zwłokach. W obu tych metodach najistotniejszą kwestią jest poprawna identyfikacja gatunków. W poniższym artykule zaprezentowane zostały molekularne metody identyfikacji, takie jak barkoding DNA czy analiza krzywych denaturacji DNA o wysokiej rozdzielczości (DNA-HRM-PCR).
Forensic entomology uses insects to determine the time, cause and place of death. To this end, two entomological methods are used. The development-based method uses the patterns of insect larvae development under the specific thermal and environmental conditions. The succession-based method analyzes the sequence of insect succession on the body in various environmental conditions. The proper insect species identification is essential in both methods. In this article, the molecular methods of species, age and sex identification are presented such as DNA barcoding or DNA-HRM-PCR.
Źródło:
Problemy Kryminalistyki; 2014, 286; 22-26
0552-2153
Pojawia się w:
Problemy Kryminalistyki
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molekularna identyfikacja gatunków z rodzaju Abies na podstawie polimorfizmu DNA mitochondrialnego
Molecular identification of species from Abies genus based on the mitochondrial DNA polymorphism
Autorzy:
Pawlaczyk, E.M.
Staniak, J.
Maliński, T.
Bobowicz, M.A.
Powiązania:
https://bibliotekanauki.pl/articles/989708.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
drzewa lesne
genetyka roslin
jodla
Abies
gatunki roslin
identyfikacja
haplotypy
DNA mitochondrialny
polimorfizm DNA
abies species
haplotype
capillary electrophoresis
mitochondrial marker
Opis:
The plant material was collected on 34 individuals growing in the Dendrological Garden of Poznań University of Life Sciences (52°25'32,95" N 16°53'39,83" E) and Botanical Garden of Adam Mickiewicz University in Poznań (52°25'11,70" N 16°52'55,07" E). The species for this study originated from Europe, Asia Minor, central and eastern Asia and North America and included: Abies alba, Abies cephalonica, Abies cilicica, Abies equi−trojani, Abies sibirica, Abies koreana, Abies pinsapo, Abies ×insignis, Abies bornmulleriana, Abies homolepsis, Abies holophylla, Abies grandis, Abies concolor, Abies concolor var. violacea, Abies concolor var. lowiana, Abies nordmanniana, Abies ×arnoldiana, Abies nephrolepis and Abies balsamea. The aim of this study was to define the species haplotypes (the length of allele) on the basis of nad5−4 mitochondrial DNA marker detected by capillary electrophoresis. This marker has been suggested as an easy−to−use tool to distinguish species of the Abies genus and it could be species−specific. Seven different haplotypes were identified. The first one appears in the species from Europe, Asia and North America. The second one was detected in firs from Europe and Asia Minor. A. cephalonica and A. sibirica were identified by the third haplotype, which occurs also in A. alba from the Balkan region. The fourth haplotype is characteristic for species from Asia and North America. The fifth and sixth haplotypes were identified in A. pinsapo and A. numidica. The seventh haplotype was detected only in A. holophylla. Applied marker is a very useful for verification of fir species especially allopatric species, less for parapatric ones. This marker is more helpful to exclude the species than to precisely identify them.
Źródło:
Sylwan; 2015, 159, 08; 675-683
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of Ephedra species by phylogenetic analyses using matK and ITS1 sequences
Autorzy:
Zhao, Y.S.
Xie, L.X.
Mao, F.Y.
Cao, Z.
Wang, W.P.
Zhao, Q.P.
Zhang, X.H.
Powiązania:
https://bibliotekanauki.pl/articles/56673.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Botaniczne
Opis:
In this study, the species identifications of seven Ephedra plants, including three medicinal plants from the Pharmacopoeia of the People’s Republic of China, were conducted using phylogenetic analyses, and the method’s validity was verified. The phylogenetic trees constructed from the maturase-coding gene (matK) and internal transcribed spacer 1 (ITS1) sequences showed that the former could be used for identifying five Ephedra plants, Ephedra intermedia, E. equisetina, E. antisyphilitica, E. major, and E. aphylla, but it had less power to discriminate E. sinica and E. przewalskii, while the latter could distinguish five Ephedra plants, E. przewalskii, E. equisetina, E. antisyphilitica, E. major, and E. aphylla, but it had less power to discriminate E. sinica and E. intermedia. However, when the two genes were combined, the seven species could be completely distinguished from each other, especially the medicinal plants from the others, which is significant in developing their pharmaceutical uses and in performing quality control assessments of herbal medicines. The method presented here could be applied to the analysis of processed Ephedra plants and to the identification of the botanical origins of crude drugs. Additionally, we discovered that E. equisetina and E. major were probably closely related to each other, and that E. sinica, E. intermedia, and E. przewalskii also had a close genetic relationship.
Źródło:
Acta Societatis Botanicorum Poloniae; 2016, 85, 2
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection and molecular identification of Cryptosporidium species in laboratory rats (Rattus norvegicus) in Ibadan, Nigeria
Autorzy:
Ayinmode, Adekunle Bamidele
Ogbonna, Nkeiruka Fortunate
Widmer, Giovanni
Powiązania:
https://bibliotekanauki.pl/articles/972090.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
laboratory rat
microscopy
pcr
sequencing
Cryptosporidium species
Opis:
To study the occurrence of Cryptosporidium infection in laboratory rats (Rattus norvegicus) raised for experimental usage, 134 faecal samples were obtained from two rearing houses in Ibadan and examined for the presence of Cryptosporidium oocyst using the modified acid fast staining technique. Cryptosporidium species in 2 samples positive for microscopy were further characterized by a nested polymerase chain reaction (PCR) amplifying the 18S rRNA gene. Two of 134 samples were positive for the Cryptosporidium oocysts. Sequencing of the small-subunit rRNA amplicons identified the species in the two PCR positive samples as Cryptosporidium andersoni and Cryptosporidium rat genotype. These findings showed that laboratory rat is a potential reservoir for diverse Cryptosporidium species and suggests that laboratory rats should be screened for Cryptosporidium infection prior to experiments, especially where pathogen free animals are not available. This the first report to identify Cryptosporidium species infecting laboratory rats in Nigeria.
Źródło:
Annals of Parasitology; 2017, 63, 2; 105-109
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of the suitability of mitochondrial DNA for species identification of microtraces and forensic traces
Autorzy:
Natonek-Wiśniewska, Małgorzata
Krzyścin, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1038564.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
biological traces
forensic DNA analysis
species identification of forensic DNA
species identification of biological traces
mtDNA
Opis:
The objective of the study was to demonstrate how mitochondrial DNA (mtDNA) can be used to determine the species origin of animal microtraces. The study included pieces of cat and dog hair without the root, a fragment of cooked chicken bone (0.1g), three goose down samples (0.028 g), a pork swab, a pork scratching (5×5×5 mm), and pork lard (0.22 g). DNA was isolated from all of these samples using the method appropriate for the particular source material. The extracts had DNA concentration exceeding 5.4 ng/µl with A260/280 purity range of 1.14-1.88. Next, the samples were subjected to PCR and real-time PCR with species-specific primers and primers complementary to mitochondrial DNA (mtDNA). Control reactions based on the amplification of eukaryotic-specific fragment (18S rRNA) were additionally performed. PCR and real-time PCR products for detection of species-specific mtDNA were obtained for all templates, whereas during the detection of eukaryote DNA no product was obtained for dog and cat hair only. The poor quality of the obtained DNA did not prevent the analysis. The results showed that mitochondrial DNA is suitable for identification of small or highly processed samples, in which genomic DNA often cannot be analyzed.
Źródło:
Acta Biochimica Polonica; 2017, 64, 4; 705-708
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of three species commonly known as“daqingye” by internal leaf anatomy and high-performance liquid chromatography analyses
Autorzy:
Zhang, Y.
Zhu, Y.
Chen, J.
Xia, C.
Deng, J.
Li, H.
Li, Y.
Li, J.
Liu, P.
Powiązania:
https://bibliotekanauki.pl/articles/59301.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Botaniczne
Opis:
The macroscopic and microscopic morphologies and indigo and indirubin concentration of the traditional Chinese medicine herbs Isatis indigotica Fort., Polygonum tinctorium Ait., and Baphicacanthus cusia (Nees) Bremek, all commonly known as “daqingye”, were determined and compared. The morphological analyses indicated that I. indigotica has leaves with winged petioles and no glandular hairs or crystals, P. tinctorium has leaves with membranous ocrea and clusters of calcium oxalate, and B. cusia has palisade cells in the mesophyll running over the main vein and single cells containing calcium carbonate crystals. Indigo and indirubin are chemical constituents that have been previously isolated from daqingye and were selected in this study as identification markers for high-performance liquid chromatography analysis due to their pharmacological activities. The chromatographic results showed that indigo and indirubin concentration varied significantly among the three species: high concentration of both indigo and indirubin were observed in I. indigotica, the highest concentration among the three daqingye plants was found in P. tinctorium but with low levels of indirubin, and the concentration of indigo and indirubin was quite low in B. cusia. In summary, three different species commonly known as daqingye were accurately distinguished by morphological observation, internal leaf anatomy analysis, and chromatographic analysis.
Źródło:
Acta Societatis Botanicorum Poloniae; 2018, 87, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
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