Wszystkie pola: glutathione reductase - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Glutathione level and glutathione reductase activity in serum of coronary heart disease patients
Autorzy:: Zuzak, Ewa
Horecka, Anna
Kiełczykowska, Małgorzata
Dudek, Aneta
Musik, Irena
Kurzepa, Joanna
Kurzepa, Jacek
Powiązania:: https://bibliotekanauki.pl/articles/972495.pdf
Data publikacji:: 2017
Wydawca:: Instytut Medycyny Wsi
Tematy:: glutathione reductase
glutathione
coronary heart disease
oxidative stress
Opis:: Introduction. Oxidative stress induced by the generation of excess reactive oxygen species is one of the cause of atherosclerosis finally leading to coronary heart disease (CHD). Glutathione reductase (GR), a flavoprotein antioxidant enzyme, regenerates glutathione (GSH) from its oxidized form, which is essential for scavenging of hydrogen peroxide by glutathione peroxidase. The aim of this study was to analyze the activity of GR and GSH level in serum of patients with various stages of CHD. Materials and method. Sixty consecutive patients with diagnosis of stable angina (SA, n=20), unstable angina (UA, n=20) and non-fatal myocardial infarction (MI, n=20) together with 20 healthy control subjects were enrolled. Venous blood samples were collected just before the coronary catheterization procedure (in study patients). GR activities and GSH concentration was determined using commercially available colorimetric kits. Results. The highest GR activity and GSH serum level was noticed in UA patients. However, statistical evaluation showed that only glutathione reductase activity was significantly higher in serum obtained from myocardial infarction patients, compared to stable angina pectoris patients (19.52 ± 13.88 vs 11.63 ± 5.45 nmol/min/ml for MI and SA, respectively; p<0.048). There were no significant differences in GSH between the CHD patients and controls. Conclusion. The elevated activity of glutathione reductase in serum of patients with unstable angina pectoris and myocardial infarction suggests the role of antioxidant system acute coronary syndromes.
Źródło:: Journal of Pre-Clinical and Clinical Research; 2017, 11, 2; 103-105
1898-2395
Pojawia się w:: Journal of Pre-Clinical and Clinical Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: In vitro effects of compounds isolated from Sideritis brevibracteata on bovine kidney cortex glutathione reductase
Autorzy:: Tandogan, Berivan
Güvenç, Ayşegül
Çalış, İhsan
Ulusu, Nuriye
Powiązania:: https://bibliotekanauki.pl/articles/1039826.pdf
Data publikacji:: 2011
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: inhibition
glutathione reductase
Sideritis brevibracteata
kinetic
8-hydroxyflavone glycosides
Opis:: Glutathione reductase (GR, E.C 1.6.4.2) is a flavoprotein that catalyzes NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH). The aim of this study was to investigate in vitro effects of phenolic compounds isolated from Sideritis brevibracteata on bovine kidney GR. The Sideritis species are widely found in nature and commonly used as medicinal plants. 7-O-glycosides of 8-OH-flavones (hypolaetin, isoscutellarein and 3'-hydroxy-4'-O-methylisoscutellarein) were isolated from aerial parts of Sideritis brevibracteata. These compounds inhibited bovine kidney cortex GR in a concentration-dependent manner. Kinetic characterization of the inhibition was also performed.
Źródło:: Acta Biochimica Polonica; 2011, 58, 4; 471-475
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Glutathione reductase activity correlates with concentration of extracellular matrix degradation products in synovial fluid from patients with joint diseases
Autorzy:: Średzińska, Krystyna
Galicka, Anna
Porowska, Halina
Średziński, Łukasz
Porowski, Tadeusz
Popko, Janusz
Powiązania:: https://bibliotekanauki.pl/articles/1040477.pdf
Data publikacji:: 2009
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: glutathione reductase
collagen
glycosaminoglycans
synovial fluid
Opis:: The mechanisms underlying cartilage matrix degradation in joint diseases is not fully understood but reactive oxygen species are implicated as main causative factors. Comparative studies of glutathione reductase (GR) activity in synovial fluid from patients with rheumatoid arthritis (RA), reactive arthritis (ReA) and osteoarthritis (OA) as well as correlations between GR activity and concentration of the major cartilage components in synovial fluid are presented in this study. We found significantly higher activity of GR in RA (about three-fold) and ReA (about two-fold) than in OA. In RA and ReA patients, GR activity in synovial fluid correlates negatively with the concentrations of collagen and degradation products of sulfated glycosaminoglycans. In OA patients the activity of GR was significantly lower than in RA and ReA, which positively correlated with the concentration of collagen and showed a tendency for positive correlation with the degradation products of sulfated glycosaminoglycans. Our results suggest that in RA and ReA patients increased activity of GR does not prevent the increased degradation of collagen and proteoglycans by ROS.
Źródło:: Acta Biochimica Polonica; 2009, 56, 4; 635-640
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Changes in GSH-antioxidant system induced by daunorubicin in human normal and diabetic fibroblasts.
Autorzy:: Zatorska, Agnieszka
Maszewski, Janusz
Jóźwiak, Zofia
Powiązania:: https://bibliotekanauki.pl/articles/1043460.pdf
Data publikacji:: 2003
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: glutathione S-transferase
glutathione reductase
glutathione peroxidase
daunorubicin
apoptosis
glutathione
oxidative stress
Opis:: We investigated the effect of daunorubicin on glutathione content and activity of GSH-related enzymes in cultured normal and diabetic human fibroblasts. Cells were incubated with 4 μM daunorubicin (DNR) for 2 h followed by culture in drug-free medium for up to 72 h. Treatment of diabetic cells with the drug caused a time-dependent depletion of intracellular GSH and a decrease of the GSH to total glutathione ratio. GSH depletion was accompanied by apoptotic changes in morphology of the nucleus. Analysis of GSH-related enzymes showed a significant increase of the activities of Se-dependent and Se-independent peroxidases and glutathione S-transferase. In contrast, glutathione reductase activity was reduced by 50%. Significant differences between normal and diabetic cells exposed to DNR were observed in the level of GST and Se-dependent glutathione peroxidase activities. These findings indicated that daunorubicin efficiently affects the GSH antioxidant defense system both in normal and diabetic fibroblasts leading to disturbances in glutathione content as well as in the activity of GSH-related enzymes.
Źródło:: Acta Biochimica Polonica; 2003, 50, 3; 825-835
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Glutathione and GSH-dependent enzymes in patients with liver cirrhosis and hepatocellular carcinoma
Autorzy:: Czeczot, Hanna
Ścibior, Dorota
Skrzycki, Michał
Podsiad, Małgorzata
Powiązania:: https://bibliotekanauki.pl/articles/1041298.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: malondialdehyde
glutathione S-transferase
glutathione reductase
liver cirrhosis
glutathione peroxidase
glutathione
hepatocellular carcinoma
Opis:: We investigated glutathione level, activities of selenium independent GSH peroxidase, selenium dependent GSH peroxidase, GSH S-transferase, GSH reductase and the rate of lipid peroxidation expressed as the level of malondialdehyde in liver tissues obtained from patients diagnosed with cirrhosis or hepatocellular carcinoma. GSH level was found to be lower in malignant tissues compared to adjacent normal tissues and it was higher in cancer than in cirrhotic tissue. Non-Se-GSH-Px activity was lower in cancer tissue compared with adjacent normal liver or cirrhotic tissue, while Se-GSH-Px activity in cancer was found to be similar to its activity in cirrhotic tissue and lower compared to control tissue. An increase in GST activity was observed in cirrhotic tissue compared with cancer tissue, whereas the GST activity in cancer was lower than in adjacent normal tissue. The activity of GSH-R was similar in cirrhotic and cancer tissues, but higher in cancer tissue compared to control liver tissue. An increased level of MDA was found in cancer tissue in comparison with control tissue, besides its level was higher in cancer tissue than in cirrhotic tissue. Our results show that the antioxidant system of cirrhosis and hepatocellular carcinoma is severely impaired. This is associated with changes of glutathione level and activities of GSH-dependent enzymes in liver tissue. GSH and enzymes cooperating with it are important factors in the process of liver diseases development.
Źródło:: Acta Biochimica Polonica; 2006, 53, 1; 237-242
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Intrygujący wpływ albendazolu na aktywność reduktazy glutationu w mięśniach myszy zarażonych Trichinella spiralis
The intriguing influence of albendazole on glutathione reductase activity in the muscles from Trichinella spiralis infected mice.
Autorzy:: Przydanek, A.
Boczoń, K.
Wandurska-Nowak, E.
Wojt, W.
Wojtkowiak, A.
Powiązania:: https://bibliotekanauki.pl/articles/2146462.pdf
Data publikacji:: 2004
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: aktywnosc enzymatyczna
Trichinella spiralis
miesnie
parazytologia
albendazol
myszy
reduktaza glutationowa
infekcja pasozytnicza
Opis:: A clear stimulation of the activity of glutathione reductase (RG, EC 1.6.4.2), one of the major enzymes participating in glutathione metabolism, was observed in the skeletal muscles from Trichinella spiralis infected mice. The maximal, almost twofold increase in RG activity was observed in week 5 post-infection. Administration of albendazole, an anthelmintic widely used in trichinellosis treatment, resulted in an additional, about 40% stimulation of RG activity (small, but statistically significant) on week 2 post-infection. In weeks 6 and 7 postinfection, when no stimulation of RG activity in infected mice caused by drug was observed, the drug modified the structure of that allosteric enzyme, affecting the kinetics of its substrate saturation in this way that the shape of the substrate saturation curve changed from „double sigmoidal”, typical of this enzyme kinetics in the muscles from infected mice, to hyperbolic, typical of this enzyme kinetics in the control, uninfected animals.
Źródło:: Wiadomości Parazytologiczne; 2004, 50, 2; 273-277
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Effect of temperature on oxidative stress induced by lead in the leaves of Plantago major L.
Autorzy:: Balakhnina, T.I.
Borkowska, A.
Nosalewicz, M.
Nosalewicz, A.
Wlodarczyk, T.M.
Kosobryukhov, A.A.
Fomina, I.R.
Powiązania:: https://bibliotekanauki.pl/articles/24178.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:: temperature effect
oxidative stress
lead
heat stress
oxidative destruction
leaf
Plantago major
ascorbate peroxidase
glutathione reductase
Źródło:: International Agrophysics; 2016, 30, 3
0236-8722
Pojawia się w:: International Agrophysics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Response of strawberry "Selva" plants on foliar application of sodium nitroprusside (nitric oxide donor) under saline conditions
Autorzy:: Jamali, B.
Eshghi, S.
Kholdebarin, B.
Powiązania:: https://bibliotekanauki.pl/articles/2004.pdf
Data publikacji:: 2014
Wydawca:: Instytut Ogrodnictwa
Tematy:: strawberry
Selva cultivar
salt stress
nitric oxide
antioxidative enzyme
proline
saline
sodium nitroprusside
superoxide dismutase
glutathione reductase
foliar application
Źródło:: Journal of Horticultural Research; 2014, 22, 2
2300-5009
Pojawia się w:: Journal of Horticultural Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: NADPH oxidase C is not the main source of ROS under arsenic toxicity but regulates antioxidants and metal transporters
Autorzy:: Gupta, D.
Inouhe, M.
Rodriguez-Serrano, M.
Romero-Puertas, M.
Sandalio, L.
Powiązania:: https://bibliotekanauki.pl/articles/81189.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
NADPH oxidase
arsenic
toxicity
oxidative metabolism
Arabidopsis thaliana
malondialdehyde
glutathione reductase activity
oxidative stress
reactive oxygen species
antioxidant
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Effect of priming of seeds of Medicago sativa "Bami" with gibberellic acid on germination, seedlings growth and antioxidant enzymes activity under salinity stress
Autorzy:: Younesi, O.
Moradi, A.
Powiązania:: https://bibliotekanauki.pl/articles/1828.pdf
Data publikacji:: 2014
Wydawca:: Instytut Ogrodnictwa
Tematy:: salinity
seed priming
Medicago sativa
gibberellic acid
germination
seedling growth
growth
antioxidative enzyme
alfalfa
guaiacol peroxidase
catalase
ascorbate peroxidase
glutathione reductase
Źródło:: Journal of Horticultural Research; 2014, 22, 2
2300-5009
Pojawia się w:: Journal of Horticultural Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Enzymatic antioxidant defense in isolated rat hepatocytes exposed to cadmium
Autorzy:: Skrzycki, M.
Czeczot, H.
Majewska, M.
Podsiad, M.
Karlik, W.
Grono, D.
Wiechetek, M.
Powiązania:: https://bibliotekanauki.pl/articles/30152.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: antioxidative enzyme
rat
hepatocyte
cadmium exposure
oxidative stress
lipid peroxidation
reduced glutathione
glutathione
environment contaminant
animal health
superoxide dismutase
catalase
glutathione peroxidase
glutathione transferase
glutathione reductase
Opis:: The aim of the study was the evaluation of cadmium effects on the activity of antioxidant enzymes in rat hepatocytes. The studies were conducted with isolated rat hepatocytes incubated for 1 or 2 hours in a modified (deprived of carbonates with phosphates) Williams’ E medium (MWE) in the presence of cadmium chloride (25, 50 and 200 μM). Hepatocytes incubated in the MWE medium without cadmium chloride were used as a control. The application of the modified Williams’ E medium allowed for the appearance of cadmium compounds in a soluble form that is indispensable for suitable estimation of its toxic action. There were evaluated markers of the oxidative stress such as: concentration of thiobarbiturate reactive substances (TBARS) – proportional to the level of lipid peroxidation, concentration of reduced glutathione (GSH), and the activity of antioxidant enzymes, including superoxide dismutase (SOD1 and SOD2), catalase (CAT), total glutathione peroxidase (GSHPx), selenium – dependent glutathione peroxidase (SeGSHPx), glutathione transferase (GST) and glutathione reductase (GSHR). Alterations of antioxidant enzymes activity, the level of TBARS and GSH in isolated rat hepatocytes caused by cadmium in vitro, were shown to depend on the concentration and time of exposure of cells to this metal. The increased level of TBARS and GSH was observed as well as changes in the activity of antioxidant enzymes. The activity of SOD isoenzymes and CAT was increased, whereas GSHPx and GST were decreased. These results indicate that cadmium induces oxidative stress followed by alterations in the cellular antioxidant enzyme system in isolated rat hepatocytes.
Źródło:: Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Antioxidant defence in maize cultivars triggered by the two-spotted spider mite attack and soil drought – a comparative study
Autorzy:: Czapla, A.
Grudkowska, M.
Miazek, A.
Zagdanska, B.
Kielkiewicz, M.
Powiązania:: https://bibliotekanauki.pl/articles/80869.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
antioxidant defense
maize cultivar
two-spotted spider mite
Tetranychus urticae
soil drought
oxidative stress
ascorbate peroxidase
catalase
glutathione reductase
peroxidase
polyphenol oxidase
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Wpływ karboplatyny na aktywność peroksydazy glutationu (GSH-Px), reduktazy glutationu (GR) oraz stężenie dialdehydu malonowego (MDA) w mediach hodowlanych ludzkich komórek czerniaka złośliwego linii Me45 in vitro
In vitro study of the infl uence of carboplatin on glutathione peroxidase (GSH-Px), glutathione reductase (GR) activities and malondialdehyde (MDA) concentration in human malignant melanoma Me45 media cells
Autorzy:: Bułdak, Rafał J.
Polaniak, Renata
Kukla, Michał
Kubina, Robert
Skonieczna, Magdalena
Bułdak, Magdalena
Stygar, Dominika
Sawczyn, Tomasz
Gowarzewski, Marcin
Żwirska-Korczala, Krystyna
Powiązania:: https://bibliotekanauki.pl/articles/1038289.pdf
Data publikacji:: 2011
Wydawca:: Śląski Uniwersytet Medyczny w Katowicach
Tematy:: karboplatyna
enzymy antyoksydacyjne
dialdehyd malonowy
peroksydacja lipidów
carboplatin
antioxidant enzyme activities
malondialdehyde
lipid peroxidation
Opis:: INTRODUCTION Reactive oxygen species (ROS) production in cancer cells usually occurs following anticancer drug exposure such as: etoposide, carboplatin compounds, doxorubicin and holoxan treatment. Normal and malignant cells contain a variety of free radical scavenging systems such as antioxidant enzymes that protect them from the eff ects of drugs that generate oxidative stress inside the cells. MATERIALS AND METHODS The culture of human malignant melanoma Me45 cell line was performed in standard conditions. The colonies were treated with two concentrations of carboplatin: (C1) 10 μg/ml and (C2) 100 μg/ml of the culture medium. The control group were colonies without a cytostatic drug in the culture medium. The medium was collected at 24 hours from addition of the carboplatin preparation and used for determination of activity of the following antioxidative enzymes: glutathione peroxidase (GSH-Px), glutathione reductase (GR), as well as the concentration of the malondialdehyde (MDA). RESULTS After 24 hours in the carboplatin treated group (C1-C2) glutathione peroxidase (GSH-Px) media activity was signifi cantly increased in concentration dependent manner in comparison to the control group (K); (125.2 ± 12.1 IU/l, 99.1 ± 13.3 IU/l vs 91.6 ± 12.1 IU/l, respectively, p < 0.001; p < 0.05). Gluthatione reductase (GR) activity was decreased in carboplatin treated groups (C1) with respect to control group (K); (8.49 ± 0.31 IU/l vs 9.49 ± 0.49 IU/l; p < 0.05). MDA media cells level was almost a 2-fold lower in carboplatin (C1) treated group compared with untreated control group (K); (0.71 ± 0.7 μmol/ml, 1.79 ± 0.15 μmol/ml vs 1.49 ± 0.11 μmol/ml, p < 0.05). CONCLUSION Carboplatin at the concentration of 10 μg/ml leads to an increase antioxidative enzyme activity (GSH-Px) and prevent to lipid peroxidation in malignant melanoma Me45 media cells.
WSTĘP Wytwarzanie reaktywnych form tlenu (RFT) w komórkach nowotworowych zwykle następuje podczas ekspozycji na leki cytostatyczne, takie jak: karboplatyna, etopozyd, doksorubicyna oraz holoksan. Prawidłowe i nowotworowe komórki mają różne systemy zmiataczy wolnych rodników, w tym: enzymy antyoksydacyjne, które chronią je przed generowanym przez cytostatyki stresem oksydacyjnym we wnętrzu komórek. MATERIAŁ I METODY Hodowla ludzkich komórek czerniaka złośliwego linii Me45 była prowadzona w standardowych warunkach. Kolonie komórek były traktowane karboplatyną w dwóch różnych stężeniach (C1) 10 μg/ml medium oraz (C2) 100 μg/ml medium. Grupę kontrolną stanowiły komórki niezawierające w medium hodowlanym leku cytostatycznego. Medium zbierano po 24 godzinach od podania preparatu, wirowano i w supernatantach oznaczano aktywności badanych enzymów antyoksydacyjnych: peroksydazy glutationu (GSH-Px) i reduktazy glutationu (GR), oraz stężenie dialdehydu malonowego (MDA). WYNIKI Po 24 godzinach w grupie komórek traktowanych karboplatyną (C1 oraz C2) aktywność peroksydazy glutationu (GSH-Px) w medium hodowlanym znacząco wzrosła zależnie od stężenia w porównaniu z grupą kontrolną (K); (12512 ± 12,1 IU/l, 99,1 ± 13,3 IU/l vs 91,6 ± 12,1 IU/l, odpowiednio, p < 0,001; p < 0,05). Aktywność reduktazy glutationu (GR) była obniżona w grupie komórek traktowanych karboplatyną (C1) w odniesieniu do grupy kontrolnej (K); (8,49 ± 0,31 IU/l vs 9,49 ± 0,49 IU/l; p < 0,001). Poziom MDA w medium komórkowym był niemal 2-krotnie niższy w grupie komórek traktowanych karboplatyną w stężeniu 10 μg/ml niż w komórkach grupy kontrolnej (K); 0,71 ± 0,7 μmol/ml vs 1,49 ± 0, 11 μmol/ml, p < 0,05). WNIOSKI Karboplatyna w stężeniu 10 μg/ml zwiększa aktywność enzymu GSH-Px i zapobiega procesowi peroksydacji lipidów w mediach komórek czerniaka złośliwego linii Me45 in vitro.
Źródło:: Annales Academiae Medicae Silesiensis; 2011, 65, 3; 7-15
1734-025X
Pojawia się w:: Annales Academiae Medicae Silesiensis
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Changes in the ascorbate-glutathione system during storage of recalcitrant seeds of Acer saccharinum L.
Autorzy:: Ratajczak, E
Pukacka, S.
Powiązania:: https://bibliotekanauki.pl/articles/57429.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: ascorbate-glutathione system
ascorbate peroxidase
storage
seed
Acer saccharinum
monodehydroascorbate reductase
Opis:: Two seed lots of Acer saccharinum (recalcitrant), with an initial moisture content of 50% and 55%, were stored at +3oC for 6 months. After this time, their viability (measured as germinability) reached 100% and 30%, respectively. In embryo axes and cotyledons extracted from seeds, two major low molecular antioxidants were assayed: ascorbate (ASA and DHA) and glutathione (GSH and GSSG); and activities of enzymes of the ascorbate-glutathione cycle were measured: ascorbate peroxidase (APO) (E.C. 1.11.1.11), monodehydroascorbate reductase (MR) (E.C. 1.6.5.4), dehydroascorbate reductase (DHAR) (E.C. 1.8.5.1), and glutathione reductase (GR) (E.C. 1.6.4.2.). GSH and GSSG contents of embryo axes of stored seeds decreased, as compared to the control (fresh, non-stored seeds), but a larger decrease was observed in seeds with 30% viability. In cotyledons, a particularly high increase in the GSH content in relation to the control was observed in seeds with 100% viability, while the GSSG content was significantly lower in both stored seed lots than in the control. The ASA level was twice as high in seeds with 30% viability as in the control, both in embryo axes and in cotyledons. The activity of enzymes of the ascorbate-glutathione cycle was higher in embryo axes than in cotyledons. In embryo axes of seeds with 100% viability, enzyme activities were slightly lower than in the control, while in those of seeds with 30% viability, their activities were higher than in the control. The observed changes in activities of enzymes of the ascorbate-glutathione cycle and in ascorbate and glutathione levels suggest that the stored seeds of A. saccharinum have an active antioxidant system, which plays an important role in maintaining their viability during storage.
Źródło:: Acta Societatis Botanicorum Poloniae; 2006, 75, 1; 23-27
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Tocopherol esters inhibit human glutathione S-transferase omega
Autorzy:: Sampayo-Reyes, Adriana
Zakharyan, Robert
Powiązania:: https://bibliotekanauki.pl/articles/1041213.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: glutathione S-transferase
human
hGSTO1-1
MMA(V) reductase
Vitamin E
Opis:: Human glutathione S-transferase omega 1-1 (hGSTO1-1) is a newly identified member of the glutathione S-transferase (GST) family of genes, which also contains alpha, mu, pi, sigma, theta, and zeta members. hGSTO1-1 catalyzes the reduction of arsenate, monomethylarsenate (MMA(V)), and dimethylarsenate (DMA(V)) and exhibits thioltransferase and dehydroascorbate reductase activities. Recent evidence has show that cytokine release inhibitory drugs, which specifically inhibit interleukin-1b (IL-1b), directly target hGSTO1-1. We found that (+)-α-tocopherol phosphate and (+)-α-tocopherol succinate inhibit hGSTO1-1 in a concentration-dependent manner with IC50 values of 2 µM and 4 µM, respectively. A Lineweaver-Burk plot demonstrated the uncompetitive nature of this inhibition. The molecular mechanism behind the inhibition of hGSTO1-1 by α-tocopherol esters (vitamin E) is important for understanding neurodegenerative diseases, which are also influenced by vitamin E.
Źródło:: Acta Biochimica Polonica; 2006, 53, 3; 547-552
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Redox-regulation of S-nitrosoglutathione reductase
Autorzy:: Holzmeister, C.
Kovacs, I.
Romling, G.
Wirtz, M.
Durner, J.
Lindermayr, C.
Powiązania:: https://bibliotekanauki.pl/articles/80722.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
nitric oxide
signalling molecule
S-nitrosoglutathione
protein
glutathione level
redox signalling
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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