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    Wyświetlanie 1-11 z 11
    Tytuł:
    Cytotoxicity and genotoxicity of GO-Fe3O4 hybrid in cultured mammalian cells
    Autorzy:
    Jedrzejczak-Silicka, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/779480.pdf
    Data publikacji:
    2017
    Wydawca:
    Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
    Tematy:
    GO-Fe3O4 hybrid
    relative viability
    DNA fragmentation
    micronucleus assay
    genotoxicity
    Opis:
    The study was aimed at investigating the effect of the Fe3O4  hybrid deposited on graphene oxide (GO- Fe3O4) on the relative viability and DNA integrity. The properties of the GO-Fe3O4  hybrid were analyzed using a transmission electron microscopy (TEM), X-ray diffraction technique (XRD) and thermal gravimetric method (TGA), while the efficiency of graphene oxide covalent functionalization with iron oxide nanospheres was determined by Fourier transform infrared spectroscopy (FT-IR). L929 and MCF-7 cell lines were selected to analyze the biocompatibility of GO-Fe3O4  nanoparticles. The hybrid was tested using WST-1 and LDH leakage assays. DNA integrity was analyzed by agarose gel electrophoresis and micronucleus assay was performed to examine chromosomal damage in the exposed cell lines. The tested GO-Fe3O4  hybrid did not significantly reduce cell metabolism of L929 cells. GO-Fe3O4 hybrid particles only slightly affected the integrity of cell membranes. DNA integrity and micronucleus assays did not indicate genotoxicity of the hybrid.
    Źródło:
    Polish Journal of Chemical Technology; 2017, 19, 1; 27-33
    1509-8117
    1899-4741
    Pojawia się w:
    Polish Journal of Chemical Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dose and Time Dependent Response of HL - 60 Cells Against Newly Isolated Phenolic Compound from Pandanus odoratissimus
    Autorzy:
    Kamble, Abhaykumar
    Reddy, Chinnappa
    Kulkarni, Amitkumar
    Powiązania:
    https://bibliotekanauki.pl/articles/1194031.pdf
    Data publikacji:
    2015
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Cytotoxicity
    HL- 60
    MTT assay
    Phenolic compound
    Opis:
    Bioassay guided chromatographic purification of methanolic extract of Pandanus odoratissium leaves afforded new phenolic compound. The isolated compound was identified as 4-(4-(3,4-dimethoxyphenyl) hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate by the spectroscope methods. The puropose of this investigation was to check the effect of the phenolic compound 4-(4-(3, 4-dimethoxyphenyl)hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate isolated pure compound, the human leukemia (HL-60) cell line as model to evaluate the cytoxicity of compound on the MTT assay. The results obtained from the parameter indicates the compound has significantly reduced the viability of HL-60 cells shows LD50 values of 21.24 ± 0.5 μg/mL, 10.25 ± 0.3 μg/mL and 5.2 ± 0.6 μg/mL, upon 12, 24 and 48 hours of exposure respective indicates a dose and dependent response relationship. The present study indicate the compound is highly cytotoxic to human leukemia (HL-60) cells supports it as an effective therapeutic agent against leukemia.
    Źródło:
    World Scientific News; 2015, 10; 44-58
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of an antimutagen of 1,4-dihydropyridine series on cell survival and DNA damage in L5178Y murine sublines
    Autorzy:
    Dalivelya, O.
    Savina, N.
    Kuzhir, T.
    Buraczewska, I.
    Wojewódzka, M.
    Szumiel, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/146264.pdf
    Data publikacji:
    2006
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    1,4-dihydropyridine
    DNA repair
    neutral comet assay
    L5178Y cells
    cytotoxicity
    radioprotective effect
    Opis:
    In a series of studies it was shown that 1,4-dihydropyridine derivatives (1,4-DHP) show antimutagenic and anticlastogenic properties and accelerate repair of oxidant and ionising radiation generated DNA damage. Here, effects of one of 1,4-DHP compounds (sodium 3,5-bis-ethoxycarbonyl-2,6-dimethyl-1,4-dihydropyridine-4-carboxylate denoted as DHP) in X-irradiated L5178Y cells (murine lymphoma sublines, LY-R and LY-S) are reported. DHP treatment 1 h before, during and after X-irradiation gave a radioprotective effect in double strand break (DSB) repair competent LY-R cells: there was an increase in post-irradiation proliferation and cell viability as well as a slight acceleration of break rejoining as measured by the neutral comet assay. In the radiosensitive LY-S cells with impaired non-homologous end-joining system, the radioprotective effect was seen as enhanced growth and viability. There was, however, no effect on the DSB repair rate. Notably, there was no dependence of the biological effects on DHP concentration in the range of concentrations studied (1 nM - 100 mM), suggesting an all-or-none effect, as in cellular signaling induction observed in radioadaptation or bystander effect. We assume that DHP acts by decreasing fixation of radiation inflicted DNA damage, among others, by increasing the rate of DNA repair and enhancing the efficiency of checkpoint control. Direct confirmation of this assumption is necessary.
    Źródło:
    Nukleonika; 2006, 51, 3; 141-146
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro assay of the biological activity of bisphosphonates
    Autorzy:
    Chmielewska, E.
    Miszczyk, P.
    Kafarski, P.
    Kempińska, K.
    Wietrzyk, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/1190037.pdf
    Data publikacji:
    2015
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    bisfosfoniany
    cytotoksyczność
    in vitro
    aktywność biologiczna
    inhibitor
    bisphosphonates
    cytotoxicity
    biological activity
    Opis:
    Nowadays, the use of bisphosphonates are the gold standard of treatment of bone diseases. Due to the high utility of these compounds, scientists still search for better and better structures, with a variety of substituents at carbon’s atom and create new libraries or improve methods of their synthesis. To determine the potential effect of newly discovered bisphosphonates, for example antiosteoporotic activity, there are in vitro tests which allow to determine the half maximal inhibitory concentrations (IC50) of cells. This paper describes in detail the methodology for test the biological activity of bisphosphonates. Shows the results of biological activity for synthesized bisphosphonates in relation to the commercial-incadronate and zoledronate.
    Źródło:
    Interdisciplinary Journal of Engineering Sciences; 2014, 2, 1; 13--16
    2300-5874
    Pojawia się w:
    Interdisciplinary Journal of Engineering Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of fungi and cytotoxicity of the species: Aspergillus ochraceus, Aspergillus niger and Aspergillus flavus isolated from the air of hospital wards
    Autorzy:
    Gniadek, Agnieszka
    Krzyściak, Paweł
    Twarużek, Magdalena
    Macura, Anna B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2161861.pdf
    Data publikacji:
    2017-03-30
    Wydawca:
    Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
    Tematy:
    cytotoxicity
    airborne fungi
    MTT assay
    Aspergillus
    air sampling
    hospital environment
    Opis:
    Objectives The basic care requirement for patients with weakened immune systems is to create the environment where the risk of mycosis is reduced to a minimum. Material and Methods Between 2007 and 2013 air samples were collected from various wards of a number of hospitals in Kraków, Poland, by means of the collision method using MAS-100 Iso MH Microbial Air Sampler (Merck Millipore, Germany). The air mycobiota contained several species of fungi, and almost 1/3 of it was made up of the species of the Aspergillus genus. Sixty-one strains of species other than A. fumigatus were selected for the research purposes, namely: 28 strains of A. ochraceus, 22 strains of A. niger and 11 strains of A. flavus species. Selected fungi underwent a cytotoxicity evaluation with the application of the MTT colorimetric assay (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide). The assay assesses cell viability by means of reducing the yellow tetrazolium salt to insoluble formazan. A semi-quantitative scale for cytotoxicity grading was adopted: low cytotoxic effect (+) with half maximal inhibitory concentration (IC₅₀) for values ranging from 31.251 cm²/ml to 7.813 cm²/ml, medium cytotoxic effect (++) for values ranging from 3.906 cm²/ml to 0.977 cm²/ml and the high one (+++) for values ranging from 0.488 cm²/ml to 0.061 cm²/ml. The absence of cytotoxicity was determined when the IC₅₀ values was at ≥ 50. Results For 48 samples the analyzed fungi displayed the cytotoxic effect with A. ochraceus in 26 out of 28 cases, with 11 strains displaying the high cytotoxic effect. The lowest cytotoxicity was displayed by fungi of A. niger in 13 out of 22 cases, and the major fungi of A. flavus species were toxic (9 out of 11 cases). Conclusions A half of the fungi displayed the low cytotoxic effect. On the basis of the comparison of average cytotoxicity levels it was determined that there were significant differences in the levels of cytotoxicity of the analyzed fungi. However, such statement may not provide grounds for a definite conclusion about the compared species of fungi that display a more cytotoxic effect than others. Int J Occup Med Environ Health 2017;30(2):231–239
    Źródło:
    International Journal of Occupational Medicine and Environmental Health; 2017, 30, 2; 231-239
    1232-1087
    1896-494X
    Pojawia się w:
    International Journal of Occupational Medicine and Environmental Health
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena cytotoksyczności kopolimeru glikolidu z laktydem (PLGA) w warunkach in vitro
    Cytotoxicity of polylactide-co-glycolide (PLGA) - evaluation in vitro
    Autorzy:
    Cieślik, M.
    Król, W.
    Mertas, A.
    Morawska-Chochół, A.
    Ziąbka, M.
    Chłopek, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/284968.pdf
    Data publikacji:
    2008
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    biomateriały
    kopolimer PLGA
    cytotoksyczność
    test MTT
    test LDH
    osteoblasty ludzkie
    badania biologiczne
    biomaterials
    copolymer PLGA
    cytotoxicity
    MTT assay
    LDH assay
    human osteoblasts
    biological evaluations
    Opis:
    Celem pracy była ocena in vitro cytotoksycznego działania bioresorbowalnego kopolimeru glikolidu z laktydem (PLGA) na ludzkie osteoblasty linii hFOB 1.19 poprzez pomiar aktywności dehydrogenazy mitochondrialnej (test MTT) oraz dehydrogenazy mleczanowej (test LDH). Do badań użyto ekstrakt uzyskany po 8 dniach inkubacji kopolimeru PLGA w medium wykorzystywanym do hodowli osteoblastów. Ekstrakt ten następnie kontaktowano przez 24 oraz 48 godziny z zaadherowanymi do dna naczynia hodowlanego osteoblastami. Po upływie założonego czasu inkubacji zarówno test MTT, jak i test LDH nie wykazał cytotoksycznego działania kopolimeru PLGA na ludzkie komórki kościotwórcze.
    The aim of the work was to evaluate in vitro the cytotoxic effect of bioresorbable polylactide-co-glycolide (PLGA) on the hFOB 1.19 human osteoblastic cell line by measuring the activity of mitochondrial dehydrogenase (MTT test) and lactate dehydrogenase (LDH test). The research made use of an extract obtained after 8 days of PLGA incubation in a medium used for osteoblast culturing. The extract was then brought into contact with osteoblasts adhered to the bottom of the culture vessel for 24 and 48 hours. After the set incubation time neither the MTT test nor the LDH test showed a cytotoxic effect of PLGA on human osteogenic cells.
    Źródło:
    Engineering of Biomaterials; 2008, 11, no. 81-84; 35-39
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synthesis and anticancer activity evaluation of novel derivatives of 7-amino-4-methylquinolin-2(1H)-one
    Autorzy:
    Kubica, Krzysztof P.
    Taciak, Przemyslaw P.
    Czajkowska, Agnieszka
    Sztokfisz-Ignasiak, Alicja
    Wyrebiak, Rafal
    Mlynarczuk-Bialy, Izabela
    Malejczyk, Jacek
    Mazurek, Aleksander P.
    Powiązania:
    https://bibliotekanauki.pl/articles/895717.pdf
    Data publikacji:
    2018-08-31
    Wydawca:
    Polskie Towarzystwo Farmaceutyczne
    Tematy:
    anticancer activity
    cytotoxicity assay
    cell migration assay
    quinolin-2(1H)-one
    Opis:
    In this study we designed and synthesized sixteen new derivatives of 7-amino-4-methylquinolin-2(1H)-one with potential anticancer activity. The structures of synthesized compounds were confirmed by 1H and 13C NMR. The activity of novel substances was evaluated by cell viability assay and wound healing assay. In vitro tests for series of sixteen novel compounds were performed. The results showed that examined compounds are selective for cancer cells, but their activity for various types of cancer is different. Three of new compounds presented ability to inhibit cells migration. The novel compounds constitute a good starting point for further studies and optimization of structure for new therapeutically effective anti-cancerous drugs. Seven compounds, which showed the highest rate of cell inhibition, were selected for further studies.
    Źródło:
    Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 903-910
    0001-6837
    2353-5288
    Pojawia się w:
    Acta Poloniae Pharmaceutica - Drug Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synthesis and anticancer activity evaluation of some new derivatives of 2-(4-benzoyl-1-piperazinyl)-quinoline and 2-(4-cinnamoyl-1-piperazinyl)-quinoline.
    Autorzy:
    Kubica, Krzysztof P.
    Taciak, Przemyslaw P.
    Czajkowska, Agnieszka
    Sztokfisz-Ignasiak, Alicja
    Wyrebiak, Rafal
    Podsadni, Piotr
    Mlynarczuk-Bialy, Izabela
    Malejczyk, Jacek
    Mazurek, Aleksander P.
    Powiązania:
    https://bibliotekanauki.pl/articles/895388.pdf
    Data publikacji:
    2018-08-31
    Wydawca:
    Polskie Towarzystwo Farmaceutyczne
    Tematy:
    anticancer activity
    cytotoxicity assay
    2-quinoline
    cell migration assay
    Opis:
    In this study we designed and synthesized twenty new derivatives of 2-(4-benzoyl-1-piperazinyl)-quinoline and 2-(4-cinnamoyl-1-piperazinyl)-quinoline with potential anticancer activity. The structures of synthesized compounds were confirmed by 1H and 13C NMR spectroscopy and MS spectrometry. The activity of novel compounds was evaluated in the cell viability assay as well as in the wound healing assay. Presented data show that examined substances have anticancer activity in cell culture. Seven compounds which showed a high rate of cell growth inhibition were selected for further studies. Three of them strongly reduced growth of B16F10 cells. The novel compounds constitute a good base for further studies and optimization of structure for new therapeutically effective anti-cancerous drugs.
    Źródło:
    Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 891-901
    0001-6837
    2353-5288
    Pojawia się w:
    Acta Poloniae Pharmaceutica - Drug Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The Cytotoxicity of Some Organic Solvents on Isolated Hepatocytes in Monolayer Culture
    Autorzy:
    Zapór, L.
    Skowroń, J.
    Gołofit-Szymczak, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/91193.pdf
    Data publikacji:
    2002
    Wydawca:
    Centralny Instytut Ochrony Pracy
    Tematy:
    in vitro methods
    hepatocytes in monolayer culture
    organic solvents
    MTT assay
    kwasy organiczne
    toksykologia
    metoda in vitro
    cytotoksyczność
    hepatocyty
    Opis:
    The cytotoxic effects of volatile and water-insoluble organic solvents (ethylbenzene, tetrachloroethylene, n-hexane) were tested on isolated hepatocytes in monolayer culture by using the 3-(4,5 dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) reduction assay. All of the tested compounds inhibited metabolic activity of hepatocytes and this effect depended on the concentration of solvents in the incubatory medium. The presence of fetal calf serum in the medium did not change the cytotoxicity of xenobiotics. IC50 values calculated on the basis of the MTT assay indicated that ethylbenzene was more cytotoxic than tetrachloroethylene and n-hexane. Using hepatocyte monolayer culture and the MTT assay to assess cytotoxicity of organic solvents causes many technical problems. It seems that it cannot be used as a rapid, cheap, and credible method.
    Źródło:
    International Journal of Occupational Safety and Ergonomics; 2002, 8, 1; 121-129
    1080-3548
    Pojawia się w:
    International Journal of Occupational Safety and Ergonomics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Validation of a flow cytometry-based assay for monitoring complement-dependent cytotoxicity of an anti-CD20 biosimilar candidate antibody
    Autorzy:
    Blanco-Santana, R.
    Cedeno-Arias, M.
    Garcia-Perez, Z.
    Columbie-Gilbert, D.
    Portillo-Vaquer, A.
    Rengifo-Calzado, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/80888.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    flow cytometry
    validation
    complement-dependent cytotoxicity
    monoclonal antibody
    CD20 antigen
    biological activity
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2014, 95, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ wzmocnionego włóknami węglowymi kopolimeru glokolidu z laktydem na odpowiedź komórkową
    The impact of polylactide-co-glycolide reinforced with carbon fibres on cellular response
    Autorzy:
    Cieślik, M.
    Król, W.
    Mertas, A.
    Morawska-Chochół, A.
    Ziąbka, M.
    Chłopek, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/285677.pdf
    Data publikacji:
    2008
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    biomateriały
    kopolimer PLGA
    włókna węglowe
    cytotoksyczność
    test MTT
    test LDH
    osteoblasty ludzkie
    badania biologiczne
    biomaterials
    copolymer PLGA
    carbon fibres
    cytotoxicity
    MTT assay
    LDH assay
    human osteoblasts
    biological evaluations
    Opis:
    W pracy dokonano oceny cytotoksycznego wpływu wzmocnionego włóknami węglowymi kopolimeru glikolidu z laktydem (PLGA+CF) na ludzkie osteoblasty linii hFOB 1.19. Przeprowadzono w tym celu pomiar aktywności dehydrogenazy mitochondrialnej metodą MTT oraz dehydrogenazy mleczanowej (test LDH) w warunkach in vitro. Oba testy nie wykazały toksycznego działania badanego kompozytu na ludzkie komórki kościotwórcze.
    This work evaluates the cytotoxic impact of poly-lactide-co-glycolide reinforced with carbon fibres (PLGA+CF) on the hFOB 1.19 human osteoblastic cell line. To this end the levels of miochondrial dehydrogenase (MTT method) and lactate dehydrogenase (LDH test) were measured in vitro. Neither test showed a toxic effect of the studied composite on the human osteogenic cells.
    Źródło:
    Engineering of Biomaterials; 2008, 11, no. 81-84; 40-44
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-11 z 11

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