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    Wyświetlanie 1-13 z 13
    Tytuł:
    Significance of stigma receptivity in intergeneric cross-pollination of Salix × Populus
    Autorzy:
    Wojciechowicz, M.K.
    Zarychta, L.
    Zenkteler, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/57606.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    The pollen–stigma interaction plays an important role in reproductive process and has been continuously studied in many interspecific and intergeneric crossing experiments. The aim of this study was to investigate stigma receptivity (SR) of willow in order to determine the most suitable period for its pollination with poplar pollen and improve the effectiveness of Salix × Populus crosses. Tissue samples were examined histologically using light, epifluorescent, scanning, and transmission electron microscopy. Willow SR was determined by stigma morphological traits, test of pollen germination rate, Peroxtesmo test of peroxidase and esterase activity on stigma surface as well as papilla ultrastructure at anthesis. We have ascertained that the SR duration in willow is short, lasting from 1 to 2 DA. The poplar pollen germination rate on willow stigmas on 1 DA ranged from 26.3 to 11.2%.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2016, 85, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dependence of callus regeneration potency on the structure and presence of the suspensor of lettuce parthenogenetic proembryos cultured in vitro
    Autorzy:
    Piosik, L.
    Zenkteler, M.
    Zenkteler, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/951288.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    plant regeneration
    embryo
    suspensor
    lettuce
    Lactuca sativa
    pathogenesis
    haploid plant
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    65 years of in vitro culture in Poland
    Autorzy:
    Zenkteler, M.
    Zenkteler, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/58090.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    in vitro culture
    history
    plant tissue
    in vitro technology
    Polska
    Opis:
    This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2013, 82, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection, isolation, and preliminary characterization of bacteria contaminating plant tissue cultures
    Izolacja, wstępna charakterystyka i wykrywanie bakterii zanieczyszczających kultury roślinne in vitro
    Autorzy:
    Kaluzna, M.
    Mikicinski, A.
    Sobiczewski, P.
    Zawadzka, M.
    Zenkteler, E.
    Orlikowska, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/27869.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Opis:
    In order to limit the contamination problem in plant tissue cultures experiments on selection of media suitable for detection and isolation of bacteria contaminating plant tissue explants, and preliminary characterization of isolates were made. In the first experiment aiming at detection of bacteria in plant explants four strains representing genera most often occurring at our survey of plant tissue cultures, and earlier isolated and identified (Bacillus, Methylobacterium, Pseudomonas and Xanthomonas) were streaked on five bacteriological media (NA, King B, K, R2A and 523) and on the medium used for plant culture initiation – 1 MS with milk albumin (IM). All strains grew on all media but on K and IM at the slowest rate and on 523 medium at the fastest. The IM medium proved to be useful for immediate bacteria detection at the initial stage of culture. In the second experiment, aiming at characterization of isolates on the basis of colony growth and morphology 14 strains (Agrobacterium, Bacillus, Curtobacterium, Flavobacterium, Lactobacillus, Methylobacterium – 2 strains Mycobacterium, Paenibacillus, Plantibacterium, Pseudomonas, Stenotrophomonas, Xanthomonas, and species Serratia marcescens) were streaked on five microbiological media: KB, NBY, YDC, YNA and YPGA. All strains grew on all those media but at different rates. The only exception was the strain of Lactobacillus spp., which did not grow on King B medium. This medium allowed the detection of such characteristic traits as fluorescence (Pseudomonas) and secretion of inclusions (Stenotrophomonas). The third experiment was focussed on assessment of the sensitivity of detection of specific bacteria in pure cultures and in plant tissue cultures using standard PCR and BIO-PCR techniques with genus specific primers and 2 methods of DNA isolation. Results showed that the use of Genomic Mini kit enabled an increase of the sensitivity by 100 times as compared to extraction of DNA by boiling. Moreover, the application of BIO-PCR increased sensitivity of detection from 102 to 105 times over the standard PCR. If looking for unknown cultivable bacteria more effective detection seems to be use of microbiological method enabling detection on bacteriological media single cells in the fragments of explants or in wash liquids, in which fragmented explants were shaken.
    W pracy określono możliwość wykrywania obecności bakterii najczęściej spotykanych w kulturach roślinnych in vitro, należących do różnych rodzajów, przy pomocy technik mikrobiologicznej i molekularnej. Za najbardziej przydatną do izolacji uznano pożywkę 523 a także pożywkę zawierającą 1 soli mineralnych MS stosowaną do inicjacji kultur roślinnych, z dodatkiem 0,025% albuminy mlecznej. Większą czułość wykrywania bakterii przy pomocy markerowego DNA uzyskano przy zastosowaniu techniki polegającej na preinkubacji badanego materiału na pożywkach bakteriologicznych, a następnie na izolacji DNA ze wzrostu (BIO-PCR). Przy porównaniu sposobów izolacji DNA większą czułość uzyskano stosując zestaw Genomic Mini (A&A Biotechnology) niż po zastosowaniu ekstrakcji w temperaturze wrzenia.
    Źródło:
    Acta Agrobotanica; 2013, 66, 4
    0065-0951
    2300-357X
    Pojawia się w:
    Acta Agrobotanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of devernalization on the transition from vegetative to prefloral phase of the broccoli (Brassica oleracea var. italica cv. 'Fiesta') shoot meristem
    Wpływ dewernalizacji na przejście merystemu pędu brokuła Brassica oleracea var. italica cv. 'Fiesta' z fazy wegetatywnej w fazę początku kwitnienia
    Autorzy:
    Zenkteler, E.
    Samardakiewicz, S.
    Kaluzewicz, A.
    Knaflewski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/27417.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    devernalization
    transition
    flowering
    vegetative phase
    broccoli
    Brassica oleracea var.italica
    Fiesta cultivar
    shoot
    meristem
    zonation
    Opis:
    The morphological and histological changes which occur in the cold-induced apical meristem of broccoli (B. oleracea var. italica cv. ‘Fiesta’) during transition from the vegetative to the generative phase have been demonstrated in our previous investigations. Now, a light microscopic study on the micromorphological parameters of the tunica and corpus of the meristem was undertaken to ascertain their association with devernalisation. Changes in the apex of broccoli seedlings grown for 26 days at 24oC, succeeded by 35 days of cold treatment (16oC - vernalization) followed by 14 days of warm treatment (24oC - devernalization), were studied and described. Microscopic analysis according to the new method of slide preparation showed cytohistological zonation and the spatial distribution of cells in the central and peripheral zones of the broccoli shoot meristem more precisely than possible hitherto. Comparison between subsequent stages of meristem reorganization after devernalization revealed variation in the structure of the apex during its transition to the evocation phase of flowering. The results of measurements show that the tunica width changes irregularly, increasing from its minimum value (112.39±5.78 μm) through the medium one to the maximum values (260.32±2.50), to decrease again, while its height increased and decreased sinusoidally from its minimum value (21.52±4.30μm) to the maximum one (76.98±7.44μm) and then decreasing again to the minimum value (21.52±4.30). The stratification of the tunica as well as its width and height revealed relatively low variation within the analyzed time. These structural traits correlated with cold/warm treatments may be further utilized in the broccoli breeding programme against developmental disturbances.
    Aby zbadać skutki dewernalizacji (skokowego podwyższenia temperatury po indukcji chłodem) wykonano analizę morfometryczną wierzchołków wzrostu pędu brokuła (B. oleracea var. italica cv. ‘Fiesta’). Sadzonki brokuła otrzymane z wysiewu 23 marca 2006 r., po sześciu dniach przepikowano do paletodoniczek wypełnionych substratem torfowym. Do fazy 5 liści sadzonki rosły w fitotronie (temp. 24oC), po czym były wernalizowane (temp. 16oC, 35 dni) i dewernalizowane (temp. 24oC, 14 dni). Od 27 maja do 10 czerwca w odstępach 2 dniowych pobierano wierzchołki wzrostu brokuła do analiz. Pomiary morfometryczne wykazały, że dewernalizacja spowodowała zaburzenia w przejściu z fazy wegetatywnej do fazy ewokacji kwitnienia, co przejawiało się w opóźnieniu i rozchwianiu poszerzania i spłaszczania się wierzchołka. Natomiast tendencja do stopniowego redukowania warstw tuniki (w miarę spłaszczania się wierzchołka) nie uległa zmianie. Rośliny poddane dewernalizacji, chciaż z opóźnieniem, zawiązały pąki kwiatostanowe. Zastosowanie devernalizacji u żadnej rośliny nie spowodowało całkowitego zahamowania rozwoju ‘róży’ brokuła.
    Źródło:
    Acta Agrobotanica; 2012, 65, 1
    0065-0951
    2300-357X
    Pojawia się w:
    Acta Agrobotanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Screening of Asparagus officinalis L. seeds for occurence and ploidy of twin embryos
    Autorzy:
    Zenkteler, M.
    Debowska, W.
    Knaflewski, M.
    Zenkteler, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/19220.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    We used germination tests to assess the frequency of polyembryony in 9 asparagus cultivars with a high propensity to produce double embryos with different ploidy levels: Alpha, Andreas, Boonlim, Cipres, Eposs, Helios, Limbras, Ravel and Sartaguda. Twin embryos inside a single seed were found in 3 cultivars: Eposs 2n, Ravel 2n and Sartaguda 2n, at 0.60% frequency (15 seeds with twin embryos out of 2500 seeds). Of 30 obtained seedlings, 14 were separated diploid-diploid twins, 6 were conjoined diploid pairs, 8 were separated diploid-haploid and 2 were diploid-haploid pairs conjoined in the hypocotyl region. Some embryos showed unilateral dominance of one embryo (size and shape). The haploid status of the smallest embryo was confirmed by chromosome number (n=x=10) and flow cytometry (nuclear C DNA amount 1.95 pg). The haploid obtained in this manner possessed enough vegetative vigor to undergo chromosome doubling.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2012, 54, 2
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Morphology of uredinia and urediniospores of the fungus Melampsora larici-epitea Kleb. a damaging pathogen of common osier (Salix viminalis L.) in Poland
    Morfologia urediniów i urediniospor grzyba Melampsora larici-epitea Kleb. - groźnego patogena wierzby wiciowej (Salix viminalis L.) w Polsce
    Autorzy:
    Ciszewska-Marciniak, J.
    Jedryczka, M.
    Jezowski, S.
    Przyborowski, J.
    Wojciechowicz, K.
    Zenkteler, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/26940.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    morphology
    uredinium
    urediniospore
    fungi
    Melampsora larici-epitea
    pathogen
    common osier
    Salix viminalis
    Polska
    Opis:
    Rust (Melampsora spp.) is a damaging disease of willows (Salix spp.), including common osier (S. viminalis L.). So far, the pathogens of this species found in Europe were identified as M. larici-epitea Kleb. or M. ribesii-viminalis Kleb. Moreover, a stem infecting form (SIF), deprived of a sexual stage in its life cycle was reported. The aim of this study was to find out which species of the rust fungi cause disease symptoms on common osier in Poland. The isolates from common osier were compared to the ones originating from its putative hybrids with trembling aspen (Populus tremula L.) and Simon’s poplar (P. simonii Carr.). Fungal isolates were obtained in 2008–2010 from 15 different genotypes of willows, including seven varieties of common osier (4 Swedish and 3 Polish), two landraces of common osier and six putative hybrids with poplars. Fungal isolates originated from three experiment sites, including west (Wielkopolska and Lubuskie) and north-east (Warmia) regions of Poland. To ensure the genetic uniformity, the isolates were derived from single uredinia, obtained from natural infection conditions. In all collected samples the position of uredinia was always hypophyllous. The diameter of uredinia was measured by Sigma Scan Pro software, after inoculation of four standard genotypes, including two common osier and two willow hybrids. The studies proved that the main cause of common osier rust is Melampsora larici-epitea f. typica. All studied isolates, including the ones obtained on putative hybrids, were very similar according to the size of uredinia and the size and morphology of urediniospores. The average size of a uredinium was 1.1 mm diameter and slightly differed between the isolates (from 0.9 to 1.3 mm), depending on willow genotype, the quality of plant material used for artificial inoculations and infection conditions. The average size of a typical urediniospore was 12.4 x 10.5 μm, but the sizes varied from 9.8 to 13.2 μm. Urediniospores from common osier were slightly bigger and more oval (12.5 x 10.4 μm) as compared to spores from the putative hybrids Salix x Populus (12.3 x 10.6 μm), but the differences were statistically insignificant. The spores were ovoid, globoid or angular, evenly echinulated. The size of uredinia, as well as the size and morphology of urediniospores were in full agreement with literature data for M. larici-epitea f. typica.
    Źródło:
    Acta Agrobotanica; 2010, 63, 2
    0065-0951
    2300-357X
    Pojawia się w:
    Acta Agrobotanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary results on studies of in vivo and in vitro sexual reproduction of Salix viminalis L.
    Autorzy:
    Zenkteler, M
    Wojciechowicz, M.
    Bagniewska-Zadworna, A.
    Jezowski, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/41263.pdf
    Data publikacji:
    2003
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    embryonic development
    Salix viminalis
    willow
    in vivo
    sexual reproduction
    in vitro
    self-pollination
    poplar
    Opis:
    In vivo and in vitro self-pollination of whole pistils of some clones of Salix viminalis enabled to obtain mature seeds containing cotyledonary embryos which after the transfer to MS medium developed into wholly formed seedlings. Pollination in vitro of placentae led to abundant pollen germination and formation of tubes which occasionally they were entering the ovules through micropyle. Fertilized ovules normally developed into germinable seeds. Distant pollination of stigmas in vivo and in vitro with pollen grains of Populus tremula, P.tomentosa, P. lasiocarpa showed the ability of pollen to germinate and to form tubes several hours after pollination. Some tubes penetrated the styles but did not enter into the placenta. When placentae were directly pollinated than pollen germinated abundantly and occasionally pollen tubes were found entering the micropyle. Embryological analysis of those ovules performed 3-5 days after pollination demonstrated the presence of globular embryos with several endosperm nuclei. The technique of in vitro placental pollination works well for Salix viminalis and it could probably be applied to other Salix species.
    Źródło:
    Dendrobiology; 2003, 50
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Budowa anatomiczna sadzonek pedowych rozanecznikow i powstawanie w nich korzeni przybyszowych
    Autorzy:
    Krzyminska, A
    Zenkteler, E.
    Czekalski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/878166.pdf
    Data publikacji:
    2001
    Wydawca:
    Uniwersytet Przyrodniczy w Poznaniu
    Tematy:
    budowa anatomiczna
    rozaneczniki
    korzenie przybyszowe
    sadzonki pedowe
    botanika
    Opis:
    One-year old shoots of Rhododendron ‘Cunningham’s White’ and R. ‘Nova Zembla’ from which cuttings were obtained. The cuttings consisted of epidermis, periderm, cortex, sclerenchyma, phloem, cambium, xylem and pith. Adventitious roots of cuttings of that taxa were developed throughout basal burls or directly in cambium region.
    Źródło:
    Roczniki Akademii Rolniczej w Poznaniu. Botanika; 2001, 04; 137-140
    1508-9193
    Pojawia się w:
    Roczniki Akademii Rolniczej w Poznaniu. Botanika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Jeszcze o reologii drewna
    More about wood rheology
    Autorzy:
    Zenkteler, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/818935.pdf
    Data publikacji:
    1961
    Wydawca:
    Polskie Towarzystwo Leśne
    Źródło:
    Sylwan; 1961, 105, 02
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-13 z 13

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