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Tytuł:
A comprehensive and practical reliability allocation method considering failure effects and reliability costs
Kompleksowa i praktyczna metoda alokacji niezawodności uwzględniająca skutki uszkodzeń i koszty niezawodności
Autorzy:
Yu, H.
Zhang, G.
Ran, Y.
Li, M.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/301115.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
reliability allocation
reliability cost
failure modes and effect analysis (FMEA)
relative reliability
fuzzy methods
alokacja niezawodności
koszty niezawodności
analiza przyczynowo-skutkowa
uszkodzenie
względna niezawodność
metody rozmyte
Opis:
In view of the drawbacks in existing allocation methods which are incomplete considerations and poor practicality, a comprehensive fuzzy allocation method considering failure effects and reliability costs is proposed. Fuzzy linguistics and triangular fuzzy numbers are used to evaluate the uncertainty and subjective factors in allocation process. The traditional risk priority numbers (RPNs) are modified to overcome the shortages which are the same factor weights and equal difference of failure effects in original methods. State of the arts, components intricacy and working conditions are used to construct the reliability costs model, which solves the difficulties of costs statistics and avoids the sophisticated calculations which exist in current allocation methods. The relationship between reliability costs and potential risk of subsystem is studied and the value range of it is given in this paper. A case example is given to illustrative the scientificity and practicability of proposed allocation method.
Ze względu na niedostatki istniejących metod alokacji, które nie dają pełnego obrazu problematyki i mają słabe zastosowanie w praktyce, w artykule zaproponowano kompleksową metodę alokacji opartą na logice rozmytej, uwzględniającą skutki uszkodzeń i koszty niezawodności. W pracy wykorzystano lingwistykę rozmytą i trójkątne liczby rozmyte do oceny niepewności i czynników subiektywnych w procesie alokacji. Zmodyfikowano tradycyjny wskaźnik liczby priorytetowej ryzyka (RPN), co pozwoliło na poprawę mankamentów charakteryzujących oryginalną metodę, t.j. takie same współczynniki wagowe i równoważność skutków uszkodzeń o różnym stopniu ciężkości. Na podstawie wiedzy o stanie techniki, złożoności komponentów i warunkach pracy, skonstruowano model kosztów niezawodności, który rozwiązuje trudności dotyczące sporządzania statystyki kosztów i pozwala uniknąć skomplikowanych obliczeń stosowanych w obecnych metodach alokacji. Zbadano związek między kosztami niezawodności a potencjalnym ryzykiem podsystemu, oraz podano jego zakres wartości. Prezentowane studium przypadku demonstruje możliwe zastosowania i efektywność proponowanej metody.
Źródło:
Eksploatacja i Niezawodność; 2018, 20, 2; 244-251
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A hybrid control strategy for a dynamic scheduling problem in transit networks
Autorzy:
Liu, Zhongshan
Yu, Bin
Zhang, Li
Wang, Wensi
Powiązania:
https://bibliotekanauki.pl/articles/2172126.pdf
Data publikacji:
2022
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
service reliability
transit network
proactive control method
deep reinforcement learning
hybrid control strategy
niezawodność usług
sieć tranzytowa
uczenie głębokie
kontrola hybrydowa
Opis:
Public transportation is often disrupted by disturbances, such as the uncertain travel time caused by road congestion. Therefore, the operators need to take real-time measures to guarantee the service reliability of transit networks. In this paper, we investigate a dynamic scheduling problem in a transit network, which takes account of the impact of disturbances on bus services. The objective is to minimize the total travel time of passengers in the transit network. A two-layer control method is developed to solve the proposed problem based on a hybrid control strategy. Specifically, relying on conventional strategies (e.g., holding, stop-skipping), the hybrid control strategy makes full use of the idle standby buses at the depot. Standby buses can be dispatched to bus fleets to provide temporary or regular services. Besides, deep reinforcement learning (DRL) is adopted to solve the problem of continuous decision-making. A long short-term memory (LSTM) method is added to the DRL framework to predict the passenger demand in the future, which enables the current decision to adapt to disturbances. The numerical results indicate that the hybrid control strategy can reduce the average headway of the bus fleet and improve the reliability of bus service.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2022, 32, 4; 553--567
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A Lujiatun-like dinosaurian assemblage from the Jehol Biota of Ningcheng, Inner Mongolia, Northeast China
Autorzy:
Zhang, H.
Yu, D.
Feng, Y.
Pei, R.
Zhou, C.-F.
Powiązania:
https://bibliotekanauki.pl/articles/31341389.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Tematy:
dinosaur
Jehol Biota
Ningcheng county
Yixian Formation
fossil
fauna composition
Early Cretaceous
Cretaceous
Inner Mongolia
China
Źródło:
Acta Palaeontologica Polonica; 2022, 67, 3; 617-621
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A novel method for 3D measurement of RFID multi-tag network using a machine vision system
Autorzy:
Zhuang, X.
Yu, X.
Zhao, Z.
Zhang, W.
Liu, Z.
Lu, D.
Dong, D.
Powiązania:
https://bibliotekanauki.pl/articles/221058.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
3D measurement
RFID multi-tag network
dual-CCD system
neural network
machine vision
Opis:
The three-dimensional (3D) coordinate measurement of radio frequency identification (RFID) multi-tag networks is one of the important issues in the field of RFID, which affects the reading performance of RFID multi-tag networks. In this paper, a novel method for 3D coordinate measurement of RFID multi-tag networks is proposed. A dual-CCD system (vertical and horizontal cameras) is used to obtain images of RFID multi-tag networks from different angles. The iterative threshold segmentation and the morphological filtering method are used to process the images. The template matching method is respectively used to determine the two-dimensional (2D) coordinate and the vertical coordinate of each tag. After that, the 3D coordinate of each tag is obtained. Finally, a back-propagation (BP) neural network is used to model the nonlinear relationship between the RFID multi-tag network and the corresponding reading distance. The BP neural network can predict the reading distances of unknown tag groups and find out the optimal distribution structure of the tag groups corresponding to the maximum reading distance. In the future work, the corresponding in-depth research on the neural network to adjust the distribution of tags will be done.
Źródło:
Metrology and Measurement Systems; 2018, 25, 3; 475-486
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A practical approach to the assessment of waterjet propulsion performance: the case of a waterjet-propelled trimaran
Autorzy:
Zhang, Lei
Zhang, Jia-ning
Shang, Yu-chen
Dong, Guo-xiang
Chen, Wei-min
Powiązania:
https://bibliotekanauki.pl/articles/963425.pdf
Data publikacji:
2019
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
waterjet propulsion thrust
pressure jump method
boundary element method
trimaran
negative thrust deduction
Opis:
To obtain a reasonable evaluation of the performance of waterjet propulsion at the design stage, a semi-theoretical and semi-empirical method is used to calculate the fundamental parameters of waterjet propulsion performance using an iterative approach. To calculate the ship’s resistance, a boundary element method based on three-dimensional potential flow theory is used to solve the wave-making resistance, and an empirical approach is used to evaluate the viscous resistance. Finally, the velocity and pressure of the capture area of the waterjet propulsion control volume are solved based on turbulent boundary layer theory. The iteration equation is established based on the waterjet-hull force-balance equation, and the change in the ship’s attitude and the local loss of the intake duct are considered. The performance parameters of waterjet propulsion, such as resistance, waterjet thrust, thrust deduction, and the physical quantity of the control volume, are solved by iteration. In addition, a PID-controlled free-running ship model is simulated using the RANS CFD method as a comparison. We apply the proposed approach and the RANS CFD method to a waterjetpropelled trimaran model, and the simulation process and the results are presented and discussed. Although there are some differences between the two methods in terms of the local pressure distribution and thrust deduction, the relative error in the evaluation results for the waterjet propulsion performance is generally reasonable and acceptable. This indicates that the present method can be used at the early stages of ship design without partial information about the waterjet propulsion system, and especially in the absence of a physical model of the pump.
Źródło:
Polish Maritime Research; 2019, 4; 27-38
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A quadrature-free Legendre polynomial approach for the fast modelling guided circumferential wave in anisotropic fractional order viscoelastic hollow cylinders
Autorzy:
Zhang, X.
Liang, S.
Shao, S.
Yu, J.
Powiązania:
https://bibliotekanauki.pl/articles/38615994.pdf
Data publikacji:
2021
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
guided wave
fractional order derivative
anisotropic viscoelastic hollow cylinder
quadrature-free Legendre polynomial approach
dispersion
attenuation
Opis:
Compared to the traditional integer order viscoelastic model, a fractional order derivative viscoelastic model is shown to be advantageous. The characteristics of guided circumferential waves in an anisotropic fractional order Kelvin–Voigt viscoelastic hollow cylinder are investigated by a quadrature-free Legendre polynomial approach combining the Weyl definition of fractional order derivatives. The presented approach can obtain dispersion solutions in a stable manner from an eigenvalue/eigenvector problem for the calculation of wavenumbers and displacement profiles of viscoelastic guided wave, which avoids a lot of numerical integration calculation in a traditional polynomial method and greatly improves the computational efficiency. Comparisons with the related studies are conducted to validate the correctness of the presented approach. The full three dimensional spectrum of an anisotropic fractional Kelvin–Voigt hollow cylinder is plotted. The influence of fractional order and material parameters on the phase velocity dispersion and attenuation curves of guided circumferential wave is discussed in detail. Moreover, the difference of the phase velocity dispersion and attenuation characteristics between the Kelvin–Voigt and hysteretic viscoelastic models is also illustrated. The presented approach along with the observed wave features should be particularly useful in non-destructive evaluations using waves in viscoelastic waveguides.
Źródło:
Archives of Mechanics; 2021, 73, 2; 121-152
0373-2029
Pojawia się w:
Archives of Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An early warning method for a slope based on the increment ratio of anchor cable internal force
Autorzy:
Feng, Jianhua
Chen, Jiaxin
Li, Jian
Zhang, Yu
Guo, Jianhua
Qiu, Hongyong
Powiązania:
https://bibliotekanauki.pl/articles/2203436.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Prestressed anchor cables are active reinforcement to improve slope stability. However, the anchoring is not a permanent guarantee of stability, and the slope retains a potential risk of instability. From the perspective of the internal force of anchor cables, a new early warning method for the safety of the slope is provided, and a slope analysis model is established. With the increase in the strength reduction factor, the internal force increment curves of anchor cables under different prestresses are obtained. The point corresponding to strength reduction factors λ1 and λ2 represents a warning point. Key conclusions are drawn as follows: (1) The internal force of an anchor cable can be used to judge the stability of the slope strengthened by a prestressed anchor cable. (2) A warning index based on the internal force increment ratio of anchor cables is established. (3) The internal force increment ratio of anchor cables eliminates the influence of the initial prestress and is convenient for engineering applications.
Źródło:
Archives of Civil Engineering; 2023, 69, 1; 553--569
1230-2945
Pojawia się w:
Archives of Civil Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An improved dynamic surface sliding mode method for autonomous cooperative formation control of underactuated USVS with complex marine environment disturbances
Autorzy:
Dong, Zaopeng
Qi, Shijie
Yu, Min
Zhang, Zhengqi
Zhang, Haisheng
Li, Jiakang
Liu, Yang
Powiązania:
https://bibliotekanauki.pl/articles/32909478.pdf
Data publikacji:
2022
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
underactuated USV
dynamic surface sliding mode control
lateral velocity tracking differentiator
nonlinear disturbance observer
Opis:
In this paper, a novel dynamic surface sliding mode control (DSSMC) method, combined with a lateral velocity tracking differentiator (LVTD), is proposed for the cooperative formation control of underactuated unmanned surface vehicles (USVs) exposed to complex marine environment disturbances. Firstly, in view of the kinematic and dynamic models of USVs and the design idea of a virtual control law in a backstepping approach, the trajectory tracking control problem of USVs’ cooperative formation is transformed into a stabilisation problem of the virtual control law of longitudinal and lateral velocities. Then, aiming at the problem of differential explosion caused by repeated derivation in the process of backstepping design, the first-order low-pass filter about the virtual longitudinal velocity and intermediate state quantity of position is constructed to replace differential calculations during the design of the control law, respectively. In order to reduce the steady-state error when stabilising the virtual lateral velocity control law, the integral term is introduced into the design of the sliding mode surface with a lateral velocity error, and then the second-order sliding mode surface with an integral is structured. In addition, due to the problem of controller oscillation and the role of the tracking differentiator (TD) in active disturbance rejection control (ADRC), the LVTD is designed to smooth the state quantity of lateral velocity. Subsequently, based on the dynamic model of USV under complex marine environment disturbances, the nonlinear disturbance observer is designed to observe the disturbances and compensate the control law. Finally, the whole cooperative formation system is proved to be uniformly and ultimately bounded, according to the Lyapunov stability theory, and the stability and validity of the method is also verified by the simulation results.
Źródło:
Polish Maritime Research; 2022, 3; 47-60
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An improved Otsu method for oil spill detection from SAR images
Autorzy:
Yu, F.
Sun, W.
Li, J.
Zhao, Y.
Zhang, Y.
Chen, G.
Powiązania:
https://bibliotekanauki.pl/articles/47553.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
remote sensing
oil spill
detection
Otsu's method
accident
marine transport
synthetic aperture radar
Opis:
In recent years, oil spill accidents have become increasingly frequent due to the development of marine transportation and massive oil exploitation. At present, satellite remote sensing is the principal method used to monitor oil spills. Extracting the locations and extent of oil spill spots accurately in remote sensing images reaps significant benefits in terms of risk assessment and clean-up work. Nowadays the method of edge detection combined with threshold segmenta- tion (EDCTS) to extract oil information is becoming increasingly popular. However, the current method has some limitations in terms of accurately extracting oil spills in synthetic aperture radar (SAR) images, where heterogeneous background noise exists. In this study, we propose an adaptive mechanism based on Otsu method, which applies region growing combined with both edge detection and threshold segmentation (RGEDOM) to extract oil spills. Remote sensing images from the Bohai Sea on June 11, 2011 and the Gulf of Dalian on July 17, 2010 are utilized to validate the accuracy of our algorithm and the reliability of extraction results. In addition, results according to EDCTS are used as a comparator to further explore validity. The comparison with results according to EDCTS using the same dataset demonstrates that the proposed self-adapting algorithm is more robust and boasts high-accuracy. The accuracy computing by the adaptive algorithm is significantly improved compared with EDCTS and threshold method.
Źródło:
Oceanologia; 2017, 59, 3
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Association between RBMS1 gene rs7593730 and BCAR1 gene rs7202877 and Type 2 diabetes mellitus in the Chinese Han population
Autorzy:
Kazakova, Elena
Chen, Meijun
Jamaspishvili, Esma
Lin, Zhang
Yu, Jingling
Sun, Lulu
Qiao, Hong
Powiązania:
https://bibliotekanauki.pl/articles/1038363.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
type 2 diabetes mellitus
RBMS1 gene
BCAR1 gene
single nucleotide polymorphisms
SNPscan
Opis:
Two recent studies found that RBMS1 gene rs7593730 and BCAR1 gene rs7202877 are related to type 2 diabetes. However, the association of these loci with type 2 diabetes mellitus (T2DM) has not been examined in Chinese. We performed a replication study to investigate the association of the 2 susceptibility loci with T2DM in the Chinese population. We genotyped 1961 Chinese participants (991 with T2DM and 970 controls) for each of the 2 single nucleotide polymorphisms (SNPs) rs7593730 in RBMS1 and rs7202877 near BCAR1 using SNPscan and examined their association with T2DM using logistic regression analysis. We also analyzed the correlation of the SNP alleles and clinical phenotypes. In additive model, genotype association analysis of BCAR1 rs7202877 loci revealed that the homozygous of rs7202877 GG carriers had significantly decreased T2DM risk compared to homozygous carriers of TT (P=0.038, OR 0.44, 95% CI 0.20-0.96). In the recessive model, the GG genotype GG had significantly decreased T2DM risk compared to GT+TT (P=0.043, OR 0.67, 95% CI 0.46-0.99). Allele G was statistically significantly correlated with TC (mmol/L) (P=0.036) and LDL-C (mmol/L) (P=0.007). As for rs7593730, the carriers of CT and TT genotype had significantly decreased T2DM risk compared to the carriers of CC genotype (CT: CC P=0.038, OR 0.71, 95% CI 0.51-0.98; TT: CC P=0.010, OR 0.32, 95% CI 0.13-0.76). In a dominant model, TT+CT: CC (P=0.013, OR 0.673, 95% CI 0.49-0.92) and in a recessive model, TT: CT+CC (P=0.019, OR 0.59, 95% CI 0.39-0.92). The T allele carriers had significantly decreased T2DM risk compared to the carriers of C (P=0.002, OR 0.65, 95% CI 0.50-0.86). Allele T was statistically correlated with FINS (P=0.010). In conclusion, our study showed that RBMS1 gene rs7593730 and BCAR1 gene rs7202877 were significantly associated with type 2 diabetes in the Chinese population.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 377-382
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial communities in PM2.5 and PM10 in broiler houses at different broiler growth stages in spring
Autorzy:
Zhang, J.
Li, Y.
Xu, E.
Jiang, L.
Tang, J.
Li, M.
Zhao, X.
Chen, G.
Zhu, H.
Yu, X.
Zhang, X.
Powiązania:
https://bibliotekanauki.pl/articles/2087465.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacterial communities
broilers
high-throughput sequencing
particulate matter
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 495-504
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI.
Autorzy:
Zhang, Jiayi
Dai, Jianliang
Zhao, Enpeng
Lin, Yun
Zeng, Li
Chen, Jinzhong
Zheng, Huari
Wang, Yu
Li, Xin
Ying, Kang
Xie, Yi
Mao, YuMin
Powiązania:
https://bibliotekanauki.pl/articles/1041522.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ovary
ePAD
peptidylarginine deiminase
hPADVI
Opis:
Peptidylarginine deiminase (PAD) catalyzes the post-translational modification of protein through the conversion of arginine to citrulline in the presence of calcium ions. Human, similar to rodents, has four isoforms of PAD (type I, II, III and IV/V), each of which is distinct in substrate specificity and tissue specific expression. In our large-scale sequencing project, we identified a new human PAD cDNA from a human fetal brain cDNA library. The putative protein encoded by this cDNA is designated hPADVI. Expression analysis of hPADVI showed that it is mainly expressed in adult human ovary and peripheral blood leukocytes. We conclude that hPADVI may be orthologous to mouse ePAD, basing on sequence comparison, chromosome localization and exon-intron structure analysis. PAD-mediated deimination of epithelial cell keratin resulting in cytoskeletal remodeling suggests a possible role for hPADVI in cytoskeletal reorganization in the egg and in early embryo development. This study describes a new important member of the human PAD family.
Źródło:
Acta Biochimica Polonica; 2004, 51, 4; 1051-1058
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Classification and decision-making of fully mechanized mining technology pattern for thin seam
Autorzy:
Wang, Chen
Zhang, Yu
Liu, Yong
Jiang, Chengyu
Zhang, Mingqing
Powiązania:
https://bibliotekanauki.pl/articles/2073857.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
technologia wydobycia
klasyfikacja
górnictwo
thin seam
classification
fully mechanised mining technology pattern
decision-making
Opis:
As one of the most important decision-making problems in fully mechanised mining, the corresponding mining technology pattern is the technical foundation of the working face. Characterised by complexity in a thin seam fully mechanised mining system, there are different kinds of patterns. In this paper, the classification strategy of the patterns in China is put forward. Moreover, the corresponding theoretical model using neural networks applied for patterns decision-making is designed. Based on the above, optimal selection of these patterns under given conditions is achieved. Lastly, the phased implementation plan for automatic mining pattern is designed. As a result of the industrial test, automatic mining for panel 22204 in Guoerzhuang Coal Mine is realised.
Źródło:
Archives of Mining Sciences; 2021, 66, 3; 333--349
0860-7001
Pojawia się w:
Archives of Mining Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Climatic signals in tree rings of Juniperus turkestanica in the Gulcha River Basin (Kyrgyzstan), reveals the recent wetting trend of high Asia
Autorzy:
Chen, F.
He, Q.
Bakytbek, E.
Yu, S.-L.
Zhang, R.-B.
Powiązania:
https://bibliotekanauki.pl/articles/41289.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Opis:
Drought variability of Kyrgyzstan is an important component of climate change of high Asia. Long-term information about the history of drought is, however, limited because the instrumental records are short. Here, we developed three chronologies for juniper trees (Juniperus turkestanica) under different microsite conditions in the Gulcha River Basin, Kyrgyzstan. The three chronologies (GUL, GUR and GUD) were compared with climate data which covered the study area. Growth of the GUL site correlates positively with May–June precipitation of the current growing season and September precipitation of the previous year, but negatively with temperatures of prior July, current May and July. Growth of the GUR site correlates positively with precipitation of current May and September, and negatively with temperature of current June and July. Ring width at GUD site is negatively correlated with temperature of the current May and July, and positively correlated with precipitation of prior December and current September. Response analysis shows that water availability is the main factor limiting the radial growth of juniper trees at the GUL and GUD sites. Based on the relationships derived from climate response analyses, the potential of tree-ring chronologies from this species to provide drought reconstructions in the Gulcha River Basin has been established. The GUL chronology and other moisture sensitive tree-ring series from high Asia capture the recent wetting trend. The records contribute to a growing tree-ring network for high Asia, including sites in China, Kyrgyzstan, Pakistan and Tajikistan.
Źródło:
Dendrobiology; 2015, 74
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cloning, expression and genetic transformation of sucrose phosphate synthase (SPS) gene in Saccharum spontaneum L.
Autorzy:
Zhang, Yun-Wei
Zhou, Yun-Zhuan
Lu, Hai-Bo
Zheng, Deng-Yu
Huang, Yan-Hua
Powiązania:
https://bibliotekanauki.pl/articles/952058.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
saccharum spontaneum l.
sps gene
cloning
expression
tissue culture
genetic transformation
Opis:
Sucrose phosphate synthase (SPS) is a key enzyme catalyzing sucrose metabolism in plants. In this study, we isolated the SPS cDNA from Saccharum spontaneum and designated as SsSPS (GenBank accession no. MF398541). The full-length of SsSPS cDNA was 4153-bp with an opening reading frame (ORF) of 3132 nucleotides, which encoded a 1043-amino acid protein. The nucleotide sequences alignment showed that it had 98%, 97% and 87% homology with S. officinarum, Setaria italica and Lolium perenne, respectively. Moreover, the SsSPS was detected to express in leaf and stem tissues of S. spontaneum and exhibited a predominant expression in the stem tissue. However, there was no significant difference in the expression level of SsSPS between young leaves and mature ones. Additionally, we generated transgenic S. spontaneum using Agrobacterium-mediated transformation. Our data will provide a valuable foundation for further study of the potential role of SPS in plants.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2017, 59, 2
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 86

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