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Tytuł:
Synchrotron Radiation Induced X-Ray Emission - SRIXE
Autorzy:
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1920883.pdf
Data publikacji:
1992-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.70.En
87.15.Mi
Opis:
The synchrotron radiation induced X-ray emission (SRIXE) technique was found very useful and sensitive for determination of trace elements content and distribution in different types of materials. Due to properties of synchrotron radiation the SRIXE technique became very unique and powerful for trace elements analysis. This paper describes the phenomena related to production of characteristic X-rays and principles of the method. The properties of SRIXE such as minimum detectable limit, spatial resolution, radiation damage, and depth sensitivity are also discussed. Selected applications are given to emphasize the usefulness of the technique.
Źródło:
Acta Physica Polonica A; 1992, 82, 2; 263-271
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biomedical Applications of Synchrotron X-Ray Fluorescence
Autorzy:
Kwiatek, W. M.
Powiązania:
https://bibliotekanauki.pl/articles/1931695.pdf
Data publikacji:
1994-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
07.85.+n
87.59.-e
32.30.Rj
Opis:
Since the exact role of trace elements in living organisms is still not very well known, it is a need of a careful analysis and study on trace element distributions in various biomedical materials. The conventional techniques such as X-ray fluorescence, proton-induced X-ray emission, atomic absorption spectrophotometry do not always give precise determination, especially in microstructures areas. Since synchrotron radiation is very powerful and gives the opportunity to work with micro-beams the synchrotron radiation induced X-ray emission technique has been recently developed. Due to many properties of synchrotron radiation it becomes a very useful tool in science. In this paper a characterization of different apparatus for a trace element analysis as well as several applications are described to show the usefulness of the synchrotron radiation induced X-ray emission.
Źródło:
Acta Physica Polonica A; 1994, 86, 5; 695-703
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
FTIR Study of Multifunctional Coatings
Autorzy:
Paluszkiewicz, C.
Długoń, E.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1490258.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
82.80.Gk
81.15.Pq
68.37.Yz
82.80.Dx
78.30.Er
Opis:
Nanoparticles of hydroxyapatite (HAp) or tricalcium phosphates (TCP) connected with fibrous phase create biomimetic system between the natural fibrous and ceramic materials building a bone. Chemical bonding between the implant and host tissue takes place through the phosphate layer, which is created on the bioactive implant surface when in contact with the body fluids environment. The Fourier transform infrared spectroscopy can yield microstructural information on the segment level complementary to the morphological information acquired from X-ray scattering as well as electron microscopy. The Fourier transform infrared method is applied to study thin films on different substrates. Moreover, the Fourier transform infrared microscope technique allows to obtain surface and cross-section maps in reflection and transmission modes. This leads to visualization of chemical imaging between substrates and films. In this work, the coatings with different addition of nanohydroxyapatite were deposited by electrodeposition method on titanium and Ti6Al4V alloys. Additionally, sublayers, such as $TiO_2$, were used to increase hydroxyapatite coating adhesion. The selection of suspension composition, depositing time and layer heat treatment conditions have the conclusive influence on the films parameters. All these experimental parameters were monitored during the sample preparation procedure. Changes in phase composition of biomaterials were determined by the Fourier transform infrared reflection technique based on focal plane array detection system. It has been found that results obtained by the Fourier transform infrared spectroscopy show the differences between the studied samples as well as that optimum time of HAp deposition was 90 s.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 551-554
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of Trace Element Concentration in Diabetic Rats Tissues
Autorzy:
Kowalska, J.
Krośniak, M.
Gryboś, R.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1809705.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
29.30.Ep
78.70.En
87.19.X-
Opis:
Diabetes is one of the most frequent diseases in developing countries and thus there is a significant interest in diabetes related studies. It was found that vanadium compounds have glucose-lowering properties in diabetes and therefore it is very important to estimate the vanadium dose in diabetes treatment. On the other hand, the proper estimation of vanadium concentration is important due to side effects that occur in vanadium supplementation. In this study the influence of V(IV) and V(V) compounds with different ligands on the concentration of K, Ca, Mn, Fe, Cu, and Zn in selected rat's tissues was investigated by means of proton induced X-ray emission technique. As a result of the measurements it was found that the concentration of vanadium depends on the organ. The highest value was determined in spleen while the lowest in pancreas. It was also found that the concentration of other elements depends on the presence of vanadium and its concentration. The most meaningful influence of vanadium presence was on iron concentration in spleen, on copper and zinc in kidney, and on manganese in pancreas.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 556-560
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of magnetite composite on the amount of double strand breaks induced with X-rays
Autorzy:
Wiecheć, A.
Nowicka, K.
Błażewicz, M.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1065182.pdf
Data publikacji:
2016-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.50.-a
87.53.-j
81.05.Mh
81.05.Ni
81.05.Pj
81.05.Qk
75.50.Bb
Opis:
The aim of this study was to find out if polylactide (PLA) modified with magnetite might affect the amount of DNA double strand breaks induced with X-rays. The human osteosarcoma cells (MG63) were seeded on the polystyrene cell culture dishes (PS), PLA and PLA modified with magnetite substrates. The double strand breaks were analyzed after X-ray irradiation (dose rate 2 Gy/min), in the first day of culturing. The number of double strand breaks increased in the PLA modified with magnetite, for example after 1 Gy of X-rays irradiation, double strand breaks/cell equaled: 24.5 vs. 17.5 and 17.3, for PLA modified with magnetite vs. PLA and PS, p < 0.0003. We conclude that PLA modified with magnetite changed the number of double strand breaks induced with X-rays. However, more research is needed to confirm that such composite might be considered as radiosensitizer in radiotherapy.
Źródło:
Acta Physica Polonica A; 2016, 129, 2; 174-175
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Energy Dissipation in the AFM Elasticity Measurements
Autorzy:
Klymenko, O.
Wiltowska-Zuber, J.
Lekka, M.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1809585.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
68.37.Ps
87.10.Vg
87.15.La
Opis:
Nowadays, it is well established that changes of cell stiffness observed by atomic force microscopy are linked with the cell cytoskeleton. Its structural and functional alterations are underlying major diseases such as cancer, inflammation or neurodegenerative disorders. So far, the use of atomic force microscopy is mostly focused on the determination of the Young modulus using the modified Hertz model. It can quantitatively describe the elastic properties of living cells, however, its value is burdened by the fact that cells are neither isotropic nor homogeneous material. Often, during the atomic force microscopy measurements, the hysteresis between the loading and unloading curves are observed which indicates the dissipation of an energy. In our studies, the index of plasticity was introduced to enumerate such effect during a single loading-unloading cycle. As the results show, such approach delivers an additional parameter describing the mechanical state of cell cytoskeleton. The analysis was performed on test samples where the mechanical properties of the melanoma cells were changed by glutaraldehyde and cytochalasin D treatments. The non-treated cells were compared with fibroblasts.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 548-551
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sulphur XANES Analysis of Cultured Human Prostate Cancer Cells
Autorzy:
Kwiatek, W.
Podgórczyk, M.
Paluszkiewicz, Cz.
Balerna, A.
Kisiel, A.
Powiązania:
https://bibliotekanauki.pl/articles/1812260.pdf
Data publikacji:
2008-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kd
87.19.xj
Opis:
Prostate cancer is one of the most commonly diagnosed cancers in men throughout the world. It is believed that changes to the structure of protein binding sites, altering its metabolism, may play an important role in carcinogenesis. Sulphur, often present in binding sites, can influence such changes through its chemical speciation. Hence there is a need for precise investigation of coordination environment of sulphur. X-ray absorption near edge structure spectroscopy offers such possibility. Cell culture samples offer histologically well defined areas of good homogeneity, suitable for successful and reliable X-ray absorption near edge structure analysis. This paper presents sulphur speciation data collected from three different human prostate cancer cell lines (PC-3, LNCaP and DU-145). Sulphur X-ray absorption near edge structure analysis was performed on K-edge structure. The spectra of cells were compared with those of cancerous tissue and with organic substances as well as inorganic compounds.
Źródło:
Acta Physica Polonica A; 2008, 114, 2; 463-470
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Applications of the Cracow X-Ray Microprobe in Tomography
Autorzy:
Bielecki, J.
Bożek, S.
Lekki, J.
Stachura, Z.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1809463.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.59.-e
87.57.Q-
Opis:
A nuclear microprobe at the IFJ PAN in Cracow has found numerous applications in different fields of research, mostly in biophysics, medical sciences, geology, and material research. In order to extend the research possibilities, a new X-ray microprobe was constructed. This new microprobe consists of three experimental lines dedicated to: (i) X-ray irradiation of biological specimens, (ii) elemental analysis of samples by micro X-ray fluorescence or total reflection X-ray fluorescence methods and (iii) computer microtomography. In this paper the computer microtomography line was described. The line consists of an open type Hamamatsu L9191 X-ray tube with microfocusing to about 2 μm, a high resolution X-ray sensitive CCD camera, and a precise goniometer composed of six piezoelectric motors. Depending on the required X-ray energy, the Hamamatsu tube is used with Ti, Mo, Ag, or W targets. A small focus size and short focus-to-object distance enable to obtain images of samples with a magnification of more than 1000× and resolution of the order of 2 μm. The computer microtomography measurements are carried out using home developed codes combined with commercial software. Details of the microprobe construction and preliminary results of the computer microtomography experiments are presented.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 537-541
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of Changes in Sulphur Oxidation States in Prostate Cancer Cells
Autorzy:
Czapla, J.
Kwiatek, W.
Lekki, J.
Steininger, R.
Göttlicher, J.
Powiązania:
https://bibliotekanauki.pl/articles/1490177.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kd
87.19.xj
Opis:
Prostate cancer cell lines along with selected organic and inorganic compounds used as references were studied with sulphur K-edge X-ray absorption near edge structure spectroscopy. The experiment was performed at the SUL-X beamline of the synchrotron radiation source ANKA, Karlsruhe (Germany). The sulphur was chosen for the studies because it is an essential biological element and out of many relevant factors, it is believed that it can take an important part in cancer transformations. The main goal was to determine which sulphur forms occur in prostate cancer cells and to compare these results with the ones obtained for non-cancerous cells. Therefore oxidation state of this element was analysed with S K-edge X-ray absorption near edge structure spectroscopy. The analysis of K-edge structure was done in order to investigate also the chemical structure of the elements neighbouring the central atom. The preliminary results from sulphur X-ray absorption near edge structure in prostate cancer cell line PC-3 and prostatic epithelial cell line PZ-HPV-7 (which was used as a control) show that there are various oxidation states of sulphur occurring in cells. The set of reference compounds with various sulphur oxidation states was used to establish the relation between the energy of the white line maximum and the oxidation state of sulphur. The equation of linear fit was used to compute the unknown oxidation state. In order to obtain a more detailed information the method of deconvolution of X-ray absorption near edge structure spectra was used. Experimental spectra were fitted with two Gaussian peaks and one arctangent step function. Fitting procedure was performed in Athena code and the deconvolution was used to assign the fraction of each sulphur form. The next step was to compare the results calculated for cancerous and non-cancerous cells. In this work, the first results of these studies are presented.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 497-501
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
X-Ray Absorption Near Edge Structure and Mössbauer Spectroscopy in Study of Iron Valence States in Tissues
Autorzy:
Dziedzic-Kocurek, K.
Banaś, A.
Kwiatek, W. M.
Stanek, J.
Powiązania:
https://bibliotekanauki.pl/articles/2044673.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
61.18.Fs
61.10.Ht
Opis:
X-ray absorption near edge structure Fe K-edge spectra and $\text{}^{57}$Fe Mössbauer spectra of selected standard compounds were recorded at room temperature. Valence and spin states of Fe in these samples known from Mössbauer spectroscopy were correlated with the shapes of X-ray absorption near edge structure spectra in search of possible application of X-ray absorption near edge structure spectroscopy as analytical tool determining local electronic states of iron in tissues. As an example, the X-ray absorption near edge structure spectra of healthy and cancerous tissues of prostate are shown, suggesting Fe$\text{}^{3+}$ in cancerous tissues.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 341-345
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
FTIR Microspectroscopy in Studies of DNA Damage Induced by Proton Microbeam in Single PC-3 Cells
Autorzy:
Lipiec, E.
Kowalska, J.
Lekki, J.
Wiecheć, A.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1490193.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.14.gk
87.64.km
78.30.Jw
87.53.Bn
Opis:
In recent years, the Fourier transformed infrared spectroscopy is often applied in studies of biological materials on cellular level. Undoubted advantage of this method is high sensitivity. In presented research the FTIR microspectroscopy was used to analyse the DNA damage in single PC-3 cells (prostate cancer cell line derived from bone metastases) irradiated by counted number of protons. Focused proton microbeam 2 MeV from the Van de Graaff accelerator at the Institute of Nuclear Physics, Polish Academy of Sciences, was used as an irradiation source. Four groups of single cells were irradiated with 1000, 2000, 4000, and 8000 protons per cell, respectively. Following irradiation cells were fixed in 70% ethanol and then analyzed by IR microspectroscopy. Bond analysis of IR spectra served as a base for result analysis. This research has focused on the detection of changes in DNA backbone spectral range (950-1240 $cm^{-1}$), which could be related to damages such as single and double strand breaks, DNA-DNA, and DNA-protein cross links. Switches and differences in intensity of DNA backbone bands (980-1149 $cm^{-1}$, 1151-1350 $cm^{-1}$ - symmetric and asymmetric $PO^{2-}$ stretching vibrations, as well as in 1110 $cm^{-1}$ - symmetric stretching of P-O-C band) were observed. Experimental spectra of irradiated and control cells were compared with simulated spectra generated by HyperChem software. The multivariate statistical methods of principal component analysis and hierarchical cluster analysis (Ward's method) were also performed and are discussed.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 506-509
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Surface Study of Selected Biomaterials Using Vibrational Spectroscopy
Autorzy:
Paluszkiewicz, C.
Kwiatek, W.
Długoń, E.
Wesełucha-Birczyńska, A.
Piccinini, M.
Powiązania:
https://bibliotekanauki.pl/articles/1809387.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.km
87.64.kp
87.64.Ee
87.85.jf
Opis:
Vibrational spectroscopy has been extensively used for in vitro and in vivo investigations of degradation mechanism and kinetics of different biomedical materials as well as it has been used to characterize the crystalline and amorphous domains in bio-mineralization process. Infrared and Raman spectroscopy methods are valuable tools in the biomaterials engineering allowing to study processes occurring during their preparation. In vitro tests, where the materials are immersed in simulated body fluids and/or artificial saliva, were used to evaluate the biocompatibility of biomaterials. This kind of tests are a wide range of repeatable and reproducible methods, which are regulated by international standards for commercial use and scientific development of new materials and products. The aim of this work was to examine phase composition of materials applied in dentistry. The bioactivity of such biomaterials was studied by immersing the samples in synthetic body fluid and artificial saliva. The changes were determined by the Fourier transform infrared and Raman microspectroscopy as well as scanning electron microscopy. It was found that results obtained by vibrational spectroscopy show the differences between the studied samples. Chemical reactions occurring during incubation of cements in artificial saliva as well as in synthetic body fluid result in formation of phosphates which deposit on the cement surface.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 533-536
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Elemental Mapping of Prostate Tissue by Micro-SRIXE
Autorzy:
Banaś, A.
Banaś, K.
Falkenberg, G.
Dyduch, G.
Kwiatek, W. M.
Powiązania:
https://bibliotekanauki.pl/articles/2044669.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.-t
Opis:
Synchrotron radiation-induced X-ray emission is now a proven analytical method for the determination of trace elements. Better insight of the role of trace elements in tissue samples can be gained by acquiring complete distribution maps of these elements. Our approach is based on the acquisition of digital maps. In this contribution we present two different methods for obtaining normalization matrix: first - based on argon intensity in the single spectrum and second - based on changes of beam current intensity. The region for the scan was carefully chosen according to the histological view of the sample. A total area was scanned with a step size of 15 μm in each direction and measuring time of 30 s per pixel. The thickness of the samples was 15 μm. A polycapillary was used for beam focusing. At the energy of 18 keV the beam size on the sample was approximately 15 μm which is of the order of a cell diameter.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 323-328
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of Human Lenses by Raman Microspectroscopy
Autorzy:
Paluszkiewicz, C.
Chaniecki, P.
Rękas, M.
Rajchel, B.
Piergies, N.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1398828.pdf
Data publikacji:
2016-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kp
42.55.Ye
87.64.-t
Opis:
A cataract is an opacity (clouding) of the normally clear lens which develops as a result of aging, metabolic disorders, trauma or heredity. The number of patients with cataract is increasing exponentially. This disease requires surgical intervention, to remove the cloudy lens and to introduce the eye lens polymer. In this work we will present analyses of degraded parts of human lens. Experimental materials were obtained from the lens removed during surgical intervention. These biological samples were measured using Almega XR Confocal Raman spectrometer (Thermo Scientific) with an excitation source of 785 nm laser line. The Raman vibrations in the spectral region of 650-1750 cm¯¹ were analyzed. The difference spectra revealed an excess of tryptophan, tyrosine, phenylalanine, β-sheet conformation, and molecules or molecular groups.
Źródło:
Acta Physica Polonica A; 2016, 129, 2; 244-246
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Infrared Spectroscopy in Molecular Study of the Piezoelectric Effect in Pigs Shin Bone
Autorzy:
Lipiec, E.
Kowalska, J.
Wiecheć, A.
Zieliński, P.
Kwiatek, W.
Iwaniec, M.
Powiązania:
https://bibliotekanauki.pl/articles/1490248.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.30.Jw
07.57.Ty
77.65.-j
87.50.ct
Opis:
Despite growing number of literature demonstrating the piezoelectric effect in bones at macro level there's still a lack of papers describing this effect at molecular level. In order to examine this effect more specifically, a study has been conducted to show the possibility of using infrared spectroscopy on samples contained in the electric field. This is the first known research on applying infrared spectroscopy to study the influence of electric field and compressive stress on bones' structure represented by the molecular IR spectrum. The samples used in this experiment as a model were prepared in pellet form, made from powdered pig's shin bone mixed with KBr (mass ratio of about 1:100). The spectra were obtained in transmission mode. Three different types of experiments were performed at each sample, which were subjected to: (a) fixed electric field (E < 40 kV/m), (b) variable electric field (0-40 kV/m), (c) compressive stress (P < 100 MPa). The obtained data have shown that the electric field and compressive stress on sample modified infrared spectra of the bone. Qualitative as well as quantitative changes in the spectral range between 900 $cm^{-1}$ and 1200 $cm^{-1}$ and the band at 562 $cm^{-1}$ ($PO_4^{3-}$ asymmetric and symmetric stretching, respectively) were observed. The relative area obtained by decomposition of the $PO_4^{3-}$ band reaches an extreme value in the external compressive stress (10 MPa), that occur under physiological conditions. Based on presented data, it was proved that the applied factors (electric field, compressive stress) can change the oscillation energy and the number of molecule's degrees of freedom.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 539-542
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Study of Human Osteoblast-Like MG 63 Cells Proliferation on Resorbable Polymer-Based Nanocomposites Modified with Ceramic and Carbon Nanoparticles
Autorzy:
Wiecheć, A.
Stodolak-Zych, E.
Frączek-Szczypta, A.
Błażewicz, M.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1490257.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
82.35.Np
87.17.Ee
Opis:
Polymer-based nanocomposites containing biocompatible and bioactive nanocomponents seem to be excellent materials that could be used in many biomedical applications. The aim of this study was biological evaluation of resorbable polymer-based nanocomposites (PLA, PCL) and their modifications with ceramic nanoparticles (silica - $SiO_2$, montmorillonite - MMT) or carbon nanotubes. The nanocomposites were seeded with the human osteoblast-like MG 63 cells. After 1, 3 and 7 days of incubation, Trypan blue exclusion test was used to determine the viability and number of cells. The cell population density depending on incubation time and cell population doubling time was calculated. The cell proliferation abilities on the all applied nanocomposites and on control material (polystyrene cell culture plate) were also compared. The number of cells growing on the nanocomposite surfaces increased with the incubation time. The cell viability was not decreased for all applied materials during the entire study (97-100%). The ceramic nanoparticles and carbon nanotubes modified the bone cell growth and proliferation rate. Results of this study confirm that all types of the nanocomposites are appropriate to the growing and proliferation of human osteoblast-like cells.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 546-550
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary Study on Chemical Speciation of Sulphur in Cancerous Tissues
Autorzy:
Kwiatek, W. M.
Banaś, A.
Banaś, K.
Kisiel, A.
Cinque, G.
Falkenberg, G.
Powiązania:
https://bibliotekanauki.pl/articles/2044744.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.Gb
Opis:
Sulphur is vital for almost all living organisms by participating in a wide variety of metabolic processes. Nevertheless its biochemistry is only partially understood, due to it a few tools such as X-ray absorption near edge structure may be used to determine its chemical speciation in biological system. In this paper we focused on analysis of composition and elemental distribution in tissue structures of biological samples. We examined cancerous and non-cancerous prostate tissues. Experiments were carried out on the L-beam line at the HASYLAB, DESY (Hamburg, Germany) and DAΦNE-Light Facility at the Laboratori Nationali di Frascati (Frascati, Italy). Synchrotron radiation induced X-ray emission measurements reveal the significant differences in concentration of sulphur between cancerous and non-cancerous tissues. X-ray absorption near edge structure spectra show two main types of sulphur that are represented by X-ray peaks at 2476 eV and 2484 eV. This suggests that different sulphur components are present in the measured samples.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 383-387
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of Corrosion Products on Fe and Cu Metals using Spectroscopic Methods
Autorzy:
Święch, D.
Paluszkiewicz, C.
Piergies, N.
Pięta, E.
Lelek-Borkowska, U.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1030643.pdf
Data publikacji:
2018-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
33.20.Ea
82.80.Gk
42.55.Ye
Opis:
In this study, the Fourier-transform infrared absorption and the Raman spectroscopies for analysis of corrosion products formed on the Fe and Cu metal surfaces after deposition in the chloride containing solution, were used. The obtained spectral patterns show that main constituent species of the corrosion products for Fe metal sample is lepidocrocite, while in the case of Cu surface there is formed mainly paratacamite. The obtained results confirm that application of vibrational spectroscopic methods is precise tool for identification and analysis of the corrosion products.
Źródło:
Acta Physica Polonica A; 2018, 133, 2; 286-288
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of Methods in Studies of Cell Death Mechanisms
Autorzy:
Borkowska, A.
Nowakowski, M.
Miszczyk, J.
Lipiec, E.
Wiltowska-Zuber, J.
Rawojć, K.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1030322.pdf
Data publikacji:
2018-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
growth and division
cell processes
fluorescence
optimization
Opis:
While studying the influence of ionizing radiation or certain chemical agents on cells, it is crucial to not only determine cytotoxicity, but also to follow cell death mechanisms. There are different methods to screen processes of cell death and still very important question remains unanswered about differences in results that could be caused by various experimental steps in procedures. Based on literature review two protocols of cell death determination were compared. First protocol regarded collecting cells floating in medium before trypsinization and following centrifugation of them. In the second protocol floating cells were discarded and attached ones were stained and fixed. In all experiments three different untreated cell lines (A172, DU145 as cancer cell lines and in comparison, fibroblasts (FB CCL 110), as a non- cancerous cell line) were used to test applied protocols. Cells were cultured and death processes were examined at different time points up to 120 h. Compared protocols showed statistically significant differences, especially in terms of necrosis, which was higher when included floating cells from culture medium and then centrifuging them. Therefore, presented results show importance of choosing a valid experimental procedure in case of evaluating cells viability and types of cell death pathways quantitatively.
Źródło:
Acta Physica Polonica A; 2018, 133, 2; 263-266
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of μ-FTIR-SR Spectroscopy to Prostate Tissue Analysis
Autorzy:
Kwiatek, W.
Paluszkiewicz, C.
Banaś, A.
Kisiel, A.
Podgórczyk, M.
Marcelli, A.
Cestelli Guidi, M.
Piccinini, M.
Powiązania:
https://bibliotekanauki.pl/articles/1810277.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.km
87.64.kp
87.64.Ee
87.85.jf
Opis:
The infrared spectromicroscopy is a quite recent technique rapidly developing thanks to the availability of new instruments and new brilliant synchrotron radiation sources in different areas and in particular to biomedical researches. In order to achieve a diffraction limited spatial resolution in tissue samples, we performed experiments at SINBAD, the synchrotron infrared beamline of the Laboratori Nazionali di Frascati. We characterized the chemical composition of prostate tissue samples taken from patients affected by prostate cancer disease. Different sizes of the pinholes were considered for the measurements. In the case of prostate tissue sections the results show the possibility to determine the intensity ratio of the $CH_2$ and $CH_3$ bands set at 2930 $cm^{-1}$ and 2960 $cm^{-1}$, respectively. Experiments were also performed with a pinhole of 5 μm of diameter and the differences in both histological and chemical compositions of such samples were determined.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 602-605
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distinguishing Prostate Cancer from Hyperplasia
Autorzy:
Kwiatek, W. M.
Banaś, A.
Banaś, K.
Podgórczyk, M.
Dyduch, G.
Falkenberg, G.
Gajda, M.
Cichocki, T.
Powiązania:
https://bibliotekanauki.pl/articles/2044731.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.-t
Opis:
The prostate gland is the most common site of neoplastic disorders in men. The knowledge of the pathogenesis of benign prostate hyperplasia and prostate cancer is still under investigation. X-ray fluorescence analysis using synchrotron radiation seems to be an appropriate technique to study the elemental composition of biological tissues. The measurements presented in this paper were carried out on the L-beam line at the HASYLAB, DESY (Germany). The observed concentrations of Fe, Cu, Mn, and Zn are significantly higher in cancerous tissues, as compared to normal and hyperplastic ones. Our results reveal also the existence of the two types of hyperplasia.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 377-381
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chemical Composition of Atherosclerotic Plaques οf apoE/LDLR-Double Knockout Mice by Synchrotron Radiation FTIR Microspectroscopy
Autorzy:
Kowalska, J.
Gajda, M.
Kwiatek, W.
Franczyk-Żarów, M.
Kostogrys, R.
Chłopicki, S.
Sandt, C.
Dumas, P.
Powiązania:
https://bibliotekanauki.pl/articles/1490259.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.19.xr
87.64.km
87.64.-t
Opis:
Atherosclerosis is a multietiological inflammatory disease of large and medium-sized arteries of increasing incidence in westernized countries. The aim of this study was to identify the biochemical changes during the progression of atherosclerosis by synchrotron radiation Fourier transform infrared microspectroscopy in atheromas of apoE/$LDLR^{-//-}$ mice fed egg-rich diet supplemented or not with angiotensin converting enzyme inhibitor perindopril. Synchrotron radiation Fourier transform infrared microspectroscopy technique was used to obtain information at high spatial resolution about the distribution of proteins (C-N, N-H, CO for amide I and amide II bands), lipids ($CH_2$, $CH_3$ bands) as well as mineral deposits (calcium carbonates and phosphates). Total contents of lipids and proteins were found to be significantly lower in animals treated with the diet and perindopril. An increase in saturation level of lipids was observed in animals fed with egg-rich diet when compared to the normal diet and perindopril treatment, which did not inhibit this effect. Moreover, a significant change in the secondary structure of proteins (ratio between absorption bands 1634 $cm^{-1}$/1656 $cm^{-1}$ attributed to β-type and α-type, respectively) was observed in both experimental groups in comparison with the control. Principal component analysis was used to analyse the recorded spectra. It has revealed that higher content of phosphates (wavenumber range 950-1020 $cm^{-1}$) was observed between egg-rich diet fed animals and the control group.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 555-560
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rozkład wybranych metali ciężkich w osadach dennych Zbiornika Dobczyckiego
Distribution of heavy metals in sediments of Dobczyce reservoir
Autorzy:
Reczynski, W.
Kwiatek, W.M.
Kubica, B.
Golas, J.
Jakubowska, M.
Niewiara, E.
Dutkiewicz, E.
Stobinski, M.
Skiba, M.
Powiązania:
https://bibliotekanauki.pl/articles/15167.pdf
Data publikacji:
2006
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
zbiornik Dobczyce
osady denne
metale ciezkie
pojemnosc sorpcyjna
stezenie pierwiastkow
arsen
kadm
chrom
miedz
Opis:
Osady denne stanowią istotny składnik środowiska wodnego, akumulując składniki mineralne, w tym metale ciężkie. Zbiornik Dobczycki, będąc rezerwuarem wody pitnej dla aglomeracji krakowskiej, spełnia bardzo ważną funkcję gospodarczą. W badaniach osadów dennych Zbiornika Dobczyckiego wykonano analizę wierzchniej ich warstwy w 17 punktach pomiarowych. Pozwoliło to na opisanie dystrybucji metali w czaszy zbiornika oraz ocenę czynników warunkujących tę dystrybucję. Analizę wykonano dwoma metodami: metodą atomowej spektrometrii absorpcyjnej oraz metodą PIXE. Stwierdzono, z wykorzystaniem narzędzi statystycznych, że ze względu na stężenie metali, punkty poboru próbek można podzielić na dwie grupy: leżące poza dawnym nurtem rzeki Raby (niższe stężenia metali ciężkich) oraz usytuowane w dawnym nurcie rzeki (wyższe stężenia). Odpowiada to zmieniającym się właściwościom sorpcyjnym osadów, zależnym m.in. od uziarnienia, składu chemicznego i mineralnego oraz zawartości materii organicznej. Zastosowanie metody PIXE umożliwiło wykonanie wielopierwiastkowej analizy składu bez wstępnej procedury przygotowania próbek, z wystarczającą czułością oznaczeń, lecz mniejszą precyzją niż metoda ASA. Metody te mogą być komplementarne z punktu widzenia potrzeb analiz środowiskowych.
Sediments constitute important part of water systems, accumulating mineral compounds, such as heavy metals. Dobczyce Reservoir, being the main drinking water source for the city of Krakow, is important from the economical point of view. In the present research, analysis of elemental composition of sediment samples (upper 10 cm layer) in 17 sampling points was performed. It enabled us to analyse distribution of analysis of elements in Dobczyce Reservoir and to assess what factors influenced that distribution. The analysis was carried out by means of two instrumental methods, namely PIXE and ASA. Based on the statistical analysis of the results, it was found that all the sampling points could be divided into two groups – one situated out of the Raba river current (characterized by lower concentration of elements) and the other along the river current (higher concentration of elements). Such a division results from the differences in chemical and mineralogical composition of sediments as well as from their organic matter content and physical properties. The use of PIXE method makes it possible to analyze sediments in a multielemental mode without sample pretreatment, obtaining satisfactory sensitivity but less precision than with the use of AAS method. Both methods could be considered as complementary in environmental analysis.
Źródło:
Journal of Elementology; 2006, 11, 3
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary Investigations of Elemental Content, Microporosity, and Specific Surface Area of Porous Rocks Using PIXE and X-ray Microtomography Techniques
Autorzy:
Bielecki, J.
Bożek, S.
Dutkiewicz, E.
Hajduk, R.
Jarzyna, J.
Lekki, J.
Pieprzyca, T.
Stachura, Z.
Szklarz, Z.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1490118.pdf
Data publikacji:
2012-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
81.70.Tx
91.65.-n
47.56.+r
Opis:
Determination of physical properties of porous geological materials is of great importance for oil industry. The knowledge of rocks properties is usually obtained from porosity studies such as pore size distribution, specific surface area determination, and hydrodynamic permeability calculations. This study describes determination of elemental composition and measurements of the particular physical properties of geological samples (porous sandstone rocks) by means of the nuclear and X-ray microprobes at the Institute of Nuclear Physics, Polish Academy of Sciences in Kraków, Poland. The special emphasis has been put on the computed microtomography method. Measurements have been carried out in close cooperation with Department of Geophysics, FGGEP AGH in Kraków, Poland. Chemical composition of the Rotliegend sandstone rock samples (few millimeters diameter), extracted from a borehole at 2679.6 m, 2741.4 m and 2742.4 m depth have been investigated using the 2.2 MeV proton beam (proton induced X-ray emission technique). Next, measurements of the porosity and the specific surface area of the pore space have been carried out using the X-ray microtomography technique. Basing on microtomographic data obtained with the high spatial resolution, simulations of the fluid dynamic in the void space of porous media have been carried out. Lattice Boltzmann method in the 3DQ19 geometrical model has been used in order to predict the hydraulic permeability of the media. In order to avoid viscosity-permeability dependence the multiple-relaxation-time model with half-way bounce back boundary conditions has been used. Computing power-consuming processing has been performed with the use of modern grid infrastructure.
Źródło:
Acta Physica Polonica A; 2012, 121, 2; 474-479
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
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