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Tytuł:
Enteropatogenne typy E. coli w biegunkach niemowlęcych
Ehnteropatogennye tipy E. coli v detskikh ponosakh
Enteropathogenic types of E. coli in infantile diarrhoea
Autorzy:
Maksymowicz, M.
Opania, A.
Powiązania:
https://bibliotekanauki.pl/articles/2189549.pdf
Data publikacji:
1973
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1973, 09, 4; 405-408
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Częstość występowania przeciwciał dla nefropatologennych serotypów E.coli
The frequency of antibodies against nephropathogenic serotypes of E. coli
Autorzy:
Narbutowicz, B.
Sołdaj, H.
Powiązania:
https://bibliotekanauki.pl/articles/2189808.pdf
Data publikacji:
1970
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1970, 06, 4; 345-348
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania nad występowaniem ciepłochwiejnej enterotoksyny wśród szczepów E. coli odpowiedzialnych za zakażenia przewodu pokarmowego
Issledovanie prisutstvija termoljabil'nogo ehnterotoksina sredi shtammov E. coli otvetstvennykh za zarazhenija pishhevaritel'nogo trakta
Studies on the occurrence of thermolabile enterotoxin in E. coli strains causing gastrointestinal infections
Autorzy:
Dzierżanowska, D.
Simm, M.
Powiązania:
https://bibliotekanauki.pl/articles/2187818.pdf
Data publikacji:
1987
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1987, 23, 4; 240-245
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The number of E. coli and C. perfringens bacteria in poultry waste and subsequent phases of composting
Liczebność bakterii E. coli i C. perfringens w odpadach drobiarskich oraz kolejnych fazach ich kompostowania
Autorzy:
Cybulska, K.
Suchecka, N.
Wrońska, I.
Mahdi-Oraibi, S.
Powiązania:
https://bibliotekanauki.pl/articles/125776.pdf
Data publikacji:
2015
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
E.coli
C. perfringens
poultry waste
composting
E. coli
odpady drobiarskie
kompostowanie
Opis:
Rapidly growing commercial poultry production generates large amounts of waste. Waste that accumulates during the poultry slaughter process often remains unprocessed, becoming a serious threat to people’s health and the natural environment. Poultry production waste constitutes problems odour threat and dangerous sanitary threat. The aim of this study was to determine the population size of Escherichia coli and Clostridium perfringens in poultry waste and in successive stages of waste composting. Research material consisted of raw feathers collected immediately after the slaughter, samples of biological sludge from the centrifuge, mixture of straw, feathers and lime and processed compost. Microbiological analyses were conducted with the use of spread plate count method and the substrate was used in accordance with research standards. The size of population of microorganisms in the samples analyzed corresponded to the waste processing stage and group of microbes. The presence of C. perfringens strains was ascertained in all samples, whereas E. coli strains were identified only in raw feathers and centrifuge sludge, being most numerous in unprocessed material. A reverse trend was observed in case of C. perfringens with the highest population density in centrifuge sludge and least density in raw feathers.
Intensywnie rozwijająca się produkcja drobiarska wiąże się z generowaniem dużej ilości odpadów. Powstające produkty uboczne podczas uboju pozostawione w stanie surowym stają się poważnym zagrożeniem dla środowiska naturalnego i zdrowia ludzi. Mogą stwarzać problemy odorotwórcze oraz istotnie groźne zanieczyszczenie sanitarne. Celem przeprowadzonych badań było określenie liczebności E. coli i C. perfringens w odpadach drobiarskich oraz kolejnych etapach ich kompostowania. Materiał badawczy stanowiły świeże pióra bezpośrednio po uboju, osad biologiczny z wirówki, mieszanina słomy, pierza i wapna oraz kompost przerobiony. Analizy mikrobiologiczne wykonano metodą płytkową, wykorzystując wybiórcze podłoża zgodne z normami. Liczebność mikroorganizmów w badanych próbach zależała od fazy przerobu odpadu i analizowanej grupy drobnoustrojów. Obecność bakterii C. perfringens stwierdzono we wszystkich próbach, natomiast bakterie E. coli tylko w piórach surowych i osadzie z wirówki, przy czym najliczniej występowały w materiale nieprzetworzonym. Odwrotną tendencję zaobserwowano w przypadku C. perfringens, które najliczniej zasiedlały osad z wirówki, natomiast ich mniejszą liczebność stwierdzono w piórach surowych.
Źródło:
Proceedings of ECOpole; 2015, 9, 1; 19-24
1898-617X
2084-4557
Pojawia się w:
Proceedings of ECOpole
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Transformation of E. coli with plasmids coding for degradation of aromatic structure of phenols
Autorzy:
Łabużek, Sylwia
Mrozik, Agnieszka
Pająk, Jolanta
Kasiak, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1045347.pdf
Data publikacji:
1994
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1994, 41, 2; 127-128
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Strain-dependent variations in attachment of E. coli to soil particles of different sizes
Autorzy:
Pachepsky, Y.A.
Yu, O.
Karns, J.S.
Shelton, D.R.
Guber, A.K.
van Kessel, J.S.
Powiązania:
https://bibliotekanauki.pl/articles/26571.pdf
Data publikacji:
2008
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Źródło:
International Agrophysics; 2008, 22, 1; 61-66
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteomics of E.coli Nissle 1917 in response to Cocos nucifera sap and wine
Autorzy:
Chandrasekhar, K
Pramoda Kumari, J.
Powiązania:
https://bibliotekanauki.pl/articles/11862.pdf
Data publikacji:
2015
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
proteomics
Escherichia coli
plant response
coconut palm
Cocos nucifera
sap
wine
protein
probiotic
identification
scanning
Opis:
In the present study, we described the protein profile experimentally by 2D-PAGE and MALDI analysis to understand the stress mechanisms of cocoti sap and wine on E.coli Nissle 1917. We isolated one newly expressed protein from cocoti wine treated gel which is not present in both control and cocoti sap treated sample i.e. P21 prophage-derived head-stabilizing proteinVG03_ECOL6 (3n1) also called as Head protein gp3. This protein mainly activities related to the viral life cycle. It helps to attach the viral gene into host. The growth rate was delayed in cocoti wine treated E.coli Nissle 1917 when compared to control and cocoti sap treated samples. Stress mechanism induce many proteins they are involved in metabolic process, hydrolase activity, lyase activity, quinone binding, phosphotransferase system, carbohydrate metabolism, DNA binding, DNA repair, transferase activity, oxidoreductase, purine metabolism, transcription antitermination, transcription regulation and other related activities. We proved that the predicted protein structure quality, resolution, density and error plot values by QMEAN analysis. Based on these results, only two differentially expressed proteins under sap stress showed that the significant results, which were N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 1, PTPB1_ECOLI and DinI-like protein Z3305/ECs2939 in prophage CP-933VDINI1_ECO57. In case of wine stress, the differentially expressed proteins were Transcription anti-termination protein RFAH- ECO57 NusA and PUR7- eco24- phosphoribosylamidazole-succinocarboxamide synthase showed significant results. ProtParam analysis indicating that the multiple physico-chemical characters of differentially expressed proteins were differed and compared. The phylogenetic tree represents the relationship in-between the differentially expressed proteins, were showed siblings (related) as well as monophytic clade.
Źródło:
International Letters of Natural Sciences; 2015, 41
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Atrazine toxicity in marine algae Chlorella vulgaris, in E. coli lux and gfp biosensor tests
Toksyczność atrazyny w glonach Chlorella vulgaris oraz w biosensorowych testach E. coli z genami gfp i lux
Autorzy:
Matejczyk, Marzena
Kondzior, Paweł
Ofman, Piotr
Juszczuk-Kubiak, Edyta
Świsłocka, Renata
Łaska, Grażyna
Wiater, Józefa
Lewandowski, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/27311571.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
ecotoxicology
atrazine
chlorella vulgaris
bioluminescent biosensors
water contamination
ekotoksykologia
atrazyna
czujniki bioluminescencji
zanieczyszczenie wody
Opis:
Atrazine (ATR) is a widely used chlorinated herbicide from the s-triazine group. Due to the widespread use of ATR, it leaks into the environment and is detected in drinking water, exceeding the WHO-acceptable concentration of atrazine in drinking water, which is 2 μg/L. The aim of our study was to determine toxicity, protein degradation and genotoxicity of ATR at concentrations of 10; 1; 0.1; 0.01 mg/L on Chlorella vulgaris and with the application of E. coli bioluminescent biosensor strains. We measured the content of chlorophyll a, b, carotenoids in Chlorella vulgaris and the inhibition of this algae culture growth. E. coli RFM443 strains with gene constructs grpE:luxCDABE, lac:luxCDABE, recA:luxCDABE and E. coli strain MM294 trc:luxCDABE were used to determine toxicity, degradation of cellular proteins and genotoxicity. On the base of the obtained results, we concluded that ATR in the tested concentrations shows a toxic effect in relation to Chlorella vulgaris. ATR is toxic and genotoxic in E. coli RFM443 strains with grpE, lac, recA promoters and causes degradation of cellular proteins. Moreover, we have detected ATR toxicity toward the GFP protein in E. coli strain MM294-GFP. Taking into account the toxicity and genotoxicity of ATR documented in our research and in the experiments of other authors, we conclude that the presence of this herbicide in surface waters and drinking water is a serious threat to living organisms.
Atrazyna (ATR) to szeroko stosowany na całym świecie chlorowany herbicyd z grupy s-triazyn. Ze względu na powszechne stosowanie, ATR przedostaje się do środowiska i jest wykrywana w wodzie pitnej, przekraczając dopuszczalne przez WHO stężenie, które wynosi 2 μg/L. W przedstawionych badaniach określono toksyczność ATR w stężeniach 10; 1; 0.1; 0.01 mg/L na glonach Chlorella vulgaris oraz z zastosowaniem E. coli mikrobiologicznych biosensorów z genami reporterowymi gfp i lux. Toksyczność oszacowano na podstawie zawartości chlorofilu a, b, karotenoidów w Chlorella vulgaris oraz zahamowania wzrostu tej kultury alg. Szczepy E. coli RFM443 z konstruktami genowymi grpE:luxCDABE, lac:luxCDABE, recA:luxCDABE i szczep E. coli MM294 trc:luxCDABE wykorzystano do określenia toksyczności, degradacji białek komórkowych i genotoksyczności. W przeprowadzonych badaniach wykryto, że ATR w analizowanych stężeniach wykazuje działanie toksyczne w stosunku do Chlorella vulgaris. W przypadku ATR stwierdzono właściwości toksyczne i genotoksyczne oraz potencjał degradacji białek w szczepach E. coli RFM443 z promotorami grpE, lac, recA. Ponadto wykryto toksyczność ATR w stosunku do białka GFP w szczepie E. coli MM294-GFP. Biorąc pod uwagę udokumentowaną w badaniach własnych oraz w doświadczeniach innych naukowców toksyczność i genotoksyczność ATR, obecność tego herbicydu w wodach powierzchniowych i wodzie pitnej stanowi poważne zagrożenie dla organizmów żywych.
Źródło:
Archives of Environmental Protection; 2023, 49, 3; 87--99
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The properties of peroxisomal ascorbate peroxidase from sugar beet (Beta vulgaris ) and expressed in E. coli
Autorzy:
Kurnik, K.
Skorupa-Klaput, M.
Tyburski, J.
Powiązania:
https://bibliotekanauki.pl/articles/80526.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
ascorbate peroxidase
sugar-beet
Beta vulgaris
Escherichia coli
expression
peroxidase activity
protein extract
BL21 cell
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of supportive E. coli mastitis treatment on PMN chemiluminescence and subpopulations of T lymphocytes
Autorzy:
Markiewicz, H.
Krumrych, W.
Gehrke, M.
Powiązania:
https://bibliotekanauki.pl/articles/30564.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this field study was to assess the impact of a single i.m. injection of lysozyme dimer and flunixin meglumine in combination with intramammary and systemic antibiotic on chemiluminescence of PMN (polymorphonuclear leucocytes) and subpopulations of lymphocyte T in blood of cows with E. coli mastitis. Examinations were performed on 30 dairy cows affected with naturally occurring acute form of E. coli mastitis. Cows were randomly divided into three groups according to the method of treatment. The first group was treated with approved intramammary antibiotic product, the same antibiotic in i.m. injection and one injection of flunixin meglumine on the first day of therapy. Next group was treated with the same antibiotic and additionally one injection of lysozyme dimer on the first day of therapy. The third one was treated only with an antibiotic and served as a control group. Blood samples were taken before treatment and on days 3 and 7. In samples haematology indices were determined, spontaneous and opsonised zymosan stimulated CL and PMA measurements were performed and the subpopulations of T lymphocyte (CD2⁺, CD4⁺, CD8⁺) were assayed in whole blood. There was no effect of the applied supportive treatment on the value of morphological blood indices. A significant influence of the time of sample collection on the level of CL and dynamics of lymphocytes T subpopulation was demonstrated. A single injection of flunixin meglumine or lysozyme dimer on the day of the beginning of treatment of E. coli mastitis, does not affect the level of neutrophil chemiluminescence and the percentage of T lymphocytes in the blood of mastitic cows in the analysed period of time.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular recognition of glyconanoparticles by RCA and E. coli K88 - designing transports for targeted therapy
Autorzy:
Gallegos-Tabanico, Amed
Sarabia-Sainz, Jose
Sarabia-Sainz, H.
Carrillo Torres, Roberto
Guzman-Partida, Ana
Monfort, Gabriela
Silva-Campa, Erika
Burgara-Estrella, Alexel
Angulo-Molina, Aracely
Acosta-Elias, Mónica
Pedroza-Montero, Martín
Vazquez-Moreno, Luz
Powiązania:
https://bibliotekanauki.pl/articles/1038557.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
bovine serum albumin
neoglycan
nanoparticles
drug delivery
bio-recognition
Opis:
The targeted drug delivery has been studied as one of the main methods in medicine to ensure successful treatments of diseases. Pharmaceutical sciences are using micro or nano carriers to obtain a controlled delivery of drugs, able to selectively interact with pathogens, cells or tissues. In this work, we modified bovine serum albumin (BSA) with lactose, obtaining a neoglycan (BSA-Lac). Subsequently, we synthesized glyconanoparticles (NPBSA-Lac) with the premise that it would be recognized by microbial galactose specific lectins. NPBSA-Lac were tested for bio-recognition with adhesins of E. coli K88 and Ricinus communis agglutinin I (RCA). Glycation of BSA with lactose was analyzed by electrophoresis, infrared spectroscopy and fluorescence. Approximately 41 lactoses per BSA molecule were estimated. Nanoparticles were obtained using water in oil emulsion method and spheroid morphology with a range size of 300-500 nm was observed. Specific recognition of NPBSA-Lac by RCA and E. coli K88 was displayed by aggregation of nanoparticles analyzed by dynamic light scattering and atomic force microscopy. The results indicate that the lactosylated nanovectors could be targeted at the E. coli K88 adhesin and potentially could be used as a transporter for an antibacterial drug.
Źródło:
Acta Biochimica Polonica; 2017, 64, 4; 671-677
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sanitary evaluation of the water from water intake points situated in Gorzow Wielkopolski and neighbouring localities
Ocena sanitarna wody pochodzącej z ujść wodnych na terenie Gorzowa Wielkopolskiego oraz okolic
Autorzy:
Wrońska, I.
Cybulska, K.
Powiązania:
https://bibliotekanauki.pl/articles/388391.pdf
Data publikacji:
2015
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
drinking water
bacteria
E.coli
E. faecalis
water contamination
woda pitna
bakteria
E. coli
zanieczyszczenie wody
Opis:
Water is an essential component necessary for sustaining life. Nevertheless, it may pose a serious threat to human life resulting from possible occurrence of pathogenic and potentially pathogenic microorganisms. Therefore, monitoring and continuous microbial surveillance of the water intended for consumption is extremely important. The requirements referring to qualitative parameters which should be met by water are set out in the Regulation of the Minister of Health of 29 March 2007, as amended on 20 April 2010, and the EU directives. The quality of water being supplied to consumers depends on many factors, among other on the type of water intake (surface water or groundwater), efficiency of water treatment and disinfection, and technical condition of the water supply network. The analysis of the sanitary condition of drinking water has been conducted form water intakes in the city of Gorzow Wielkopolski, ie Centralny Kosynierow Gdynskich intake, Klodawa intake and Siedlice intake, and in neighbouring localities, ie. Maszewo and Marwice. The research material was raw and treated water samples. The population size of Faecal streptococci, coliform bacteria, including E. coli, and total mesophilic and psychrophilic bacteria counts was determined. The analysis of the sanitary condition of drinking water has been conducted. The number of coliform bacteria, including E. coli, and streptococci was determined by the method of membrane filters, whereas total mesophilic and psychrophilic bacteria counts using the plate method under incubation conditions conforming to the standards. The presence of mesophilic and psychrophilic bacteria was observed in all examined water intake points. Differences in their counts over the whole year being significant. In three research points, ie Centralny Kosynierow Gdynskich intake, Maszewo intake and Marwice intake, psychrophilic bacteria count was exceeded (100 CFU ۰ cm–3). In the case of pathogenic bacteria, their occurrence was occasionally observed in the drinking water intake points. The water treatment processes applied and, as the final result, water sanitary assessment according to the existing standards and the provision stipulated allow it to be collected for consumption purposes and do not pose the threat to human health and life.
Woda jest podstawowym składnikiem niezbędnym do utrzymania życia. Jednakże, może ona stworzyć poważne zagrożenie dla zdrowia człowieka wynikające z możliwości wystąpienia mikroorganizmów chorobotwórczych i potencjalnie chorobotwórczych. W związku z tym, niezwykle istotna jest kontrola oraz stały nadzór mikrobiologiczny nad wodą przeznaczoną do spożycia. Wytyczne co do parametrów jakościowych, jakim powinna odpowiadać, zawarte są w Rozporządzeniu Ministra Zdrowia z dnia 29 marca 2007 r. wraz ze zmianą z dnia 20 kwietnia 2010 r. oraz Dyrektywach Unii Europejskiej. Jakość wody dostarczanej konsumentom zależy od wielu czynników m.in.: rodzaju ujęcia (woda powierzchniowa lub podziemna), skuteczności procesów uzdatniania i dezynfekcji oraz stanu technicznego sieci wodociągowej. Przeprowadzono analizę stanu sanitarnego wody pitnej pochodzącej z ujęć wodnych zaopatrujących miasto Gorzów Wielkopolski, tj. ujęcie Centralne Kosynierów Gdyńskich, Kłodawa, Siedlice oraz sąsiadujące miejscowości, tj. Maszewo i Marwice. Materiałem badawczym były próbki wody surowej i uzdatnionej. Określono liczebność paciorkowców kałowych, bakterii z grupy coli, w tym E. coli oraz ogólnej liczebności bakterii mezofilnych i psychrofilnych. Liczebność bakterii z grupy coli, w tym E. coli oraz paciorkowców kałowych oznaczono metodą filtrów membranowych, zaś ogólną liczebność bakterii mezofilnych i psychrofilnych metodą płytkową w warunkach inkubacji zgodnych z normami. We wszystkich badanych punktach stwierdzono obecność bakterii mezofilnych i psychrofilnych. Różnice w ich liczebności na przestrzeni całego roku były istotne. W punktach badawczych, tj. Centralne Kosynierów Gdyńskich, Maszewo oraz Marwice odnotowano przekroczenie norm ilości psychrofilii 100 j.t.k. ۰ cm–3. Obecność bakterii chorobotwórczych w badanych próbkach wody pitnej wykryto sporadycznie. Zastosowane procesy uzdatniania i w efekcie końcowym ocena sanitarna wody według obowiązujących norm i zawartej klauzuli pozwalają na jej pobór do spożycia i nie stanowią zagrożenia dla zdrowia i życia ludzi.
Źródło:
Ecological Chemistry and Engineering. A; 2015, 22, 1; 17-26
1898-6188
2084-4530
Pojawia się w:
Ecological Chemistry and Engineering. A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nonspecific transmission of the NPTII gene from E. coli helper plasmid to a plasmid of Agrobacterium tumefaciens
Autorzy:
Leśniewicz, Krzysztof
Grygorowicz, Wojciech
Bartkowiak, Sławomir
Augustyniak, Halina
Powiązania:
https://bibliotekanauki.pl/articles/1045369.pdf
Data publikacji:
1994
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1994, 41, 2; 129-132
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
LBP gene methylation involved in mRNA expression and resistance to E. coli F18 in weaned piglets
Autorzy:
Gan, L.N.
Bao, W.B.
Wu, S.L.
Qin, W.Y.
Sun, L.
Zhao, C.X.
Powiązania:
https://bibliotekanauki.pl/articles/2087872.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
LBP gene
methylation analysis of the LBP region
E.coli F18 strain
weaned piglets
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 643-650
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system
Autorzy:
Mazurkiewicz-Pisarek, Anna
Mazurkiewicz, Alina
Mikiewicz, Diana
Baran, Piotr
Ciach, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/16706242.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
factor VIII
hemophilia type A
recombinant coagulation factor VIII
prokaryotic expression system
E. coli
recombinant protein production system
Opis:
In this article, we have demonstrated the feasibility of generating an active form of recombinant blood coagulation factor VIII using an E. coli bacterial expression system as a potential treatment for hemophilia type A. Factor VIII (FVIII), an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. So far, all available recombinant FVIII formulations have been produced using eukaryotic expression systems. Mammalian cells can produce catalytically active proteins with all the necessary posttranslational modifications. However, cultivating such cells is time-consuming and highly expensive, and the amount of the obtained product is usually low. In contrast to eukaryotic cells, bacterial culture is inexpensive and allows the acquisition of large quantities of recombinant proteins in a short time. With this study, we aimed to obtain recombinant blood coagulation factor VIII using the E. coli bacterial expression system, a method not previously explored for this purpose. Our research encompasses the synthesis of blood coagulation factor VIII and its expression in a prokaryotic system. To achieve this, we constructed a prokaryotic expression vector containing a synthetic factor VIII gene, which was then used for the transformation of an E. coli bacterial strain. The protein expression was confirmed by mass spectrometry, and we assessed the stability of the gene construct while determining the optimal growth conditions. The production of blood coagulation factor VIII by the E. coli bacterial strain was carried out on a quarter-technical scale. We established the conditions for isolation, denaturation, and renaturation of the protein, and subsequently confirmed the activity of FVIII.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 3; 247-262
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Studies on biosynthesis of silver nanoparticles using Rhizopus sp. and its antibacterial efficacy on E. coli MDR strains
Autorzy:
Hiremath, J.
Rathod, V.
Ninganagouda, S.
Singh, D.
Prema, K.
Powiązania:
https://bibliotekanauki.pl/articles/11479.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
biosynthesis
silver nanoparticle
Rhizopus
antibacterial activity
Escherichia coli
multidrug resistance
nanotechnology
Opis:
Nanotechnology is a field that is burgeoning day by day, making an impact in all spheres of human life. Biological methods of synthesis have paved way for the “greener synthesis” of nanoparticles and these have proven to be better methods due to slower kinetics, they offer better manipulation and control over crystal growth and their stabilization. In this context we have investigated extracellular biosynthesis of silver nanoparticles (AgNPs) using cell-free extract of Rhizopus spp.. Formation of AgNPs was indicated by the change in the colour of the cellfree extract from yellow to dark brown under static condition after 48 hrs of incubation. Characterization of AgNPs was carried out by UV-Vis Spectroscopy which gave sharp plasmon resonance peak at 429 nm corresponding to spherical shaped nanoparticles. Transmission electron microscopy (TEM) micrograph showed formation of well-dispersed AgNPs in the range of 25-50 nm. Scanning electron microscopy (SEM) showed the particles to be uniformly dispersed without agglomeration with smooth morphology. EDS showed the presence of elemental silver at 3kev. X-ray diffraction (XRD)-spectrum of the AgNPs exhibited 2θ¸ values corresponding to nanocrystal. These biosynthesized AgNPs were used to study their antimicrobial activity against Multi-drug resistant (MDR) E. coli strains, by Agar diffusion method. Zone of inhibition was measured. Synthesis of nanosized particles with antibacterial properties, which are called "nanoantibiotics", is of great interest in the development of new pharmaceutical products.
Źródło:
International Letters of Natural Sciences; 2014, 18
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Type 1 fimbriae in commensal Escherichia coli derived from healthy humans
Autorzy:
Pusz, Paweł
Bok, Ewa
Mazurek, Justyna
Stosik, Michał
Baldy-Chudzik, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/1039310.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
commensal E. coli
type 1 fimbriae
gene expression
Opis:
Type 1 fimbriae are one of the most important factors of Escherichia coli adaptation to different niches in the host. Our study indicated that the genetic marker - fimH gene occurred commonly in commensal E. coli derived from healthy humans but expression of the type 1 fimbriae was not observed. Identification of fim structural subunit genes (fimA-fimH) and recombinase fimE and fimB genes showed that many of the strains were carrying an incomplete set of genes and the genes expression study revealed that in strains with complete set of fim genes, the fimC gene, encoding the chaperone protein, was not expressed.
Źródło:
Acta Biochimica Polonica; 2014, 61, 2; 389-392
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Porównanie efektywności testu immunochromatografii i multiplex-CPR przy badaniu mięsa skażonego szczepami Salmonella Enteritidis i E. coli 0157
Autorzy:
Gryko, Romuald.
Niczyporuk, Jowita S.
Powiązania:
Biluletyn Wojskowy Instytut Chemii i Radiometrii, 2006, nr 1, s. 61-65
Data publikacji:
2006
Tematy:
Mięso skażenia badanie
Salmonella
Pałeczki okrężnicy
Opis:
Fot., tab.; Bibliogr.; Streszcz.
Dostawca treści:
Bibliografia CBW
Artykuł
Tytuł:
Microbiological air rating in a variety of objects during treatment of the post-slaughter poultry wastes Part I. Escherichia coli
Autorzy:
Oraibi, S. M.
Cybulska, K.
Powiązania:
https://bibliotekanauki.pl/articles/125448.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
E.coli
air
poultry waste facilities
composting
Opis:
The presence of Escherichia coli in the air of facilities involved in management and composting of post-slaughter poultry wastes in selected plants of West Western Pomerania region was studied. Measurements were made on four dates in a variety of weather conditions during the year. The study was conducted at 5 objects that differ in the type of waste and the degree of preparation for composting. These were: chemical treatment and preliminary processing plant, liquid wastes reservoir, platform for preparation of materials for composting, storage of biological sediments, and composting facility. Measurement of bacteria count was carried out in accordance with the applicable procedures on selective chromogenic TBX medium. The assays revealed the presence of E. coli at all test objects, but not always on all measurement dates. It has been shown that the presence of E. coli was from 20 to 3047 CFUm-3 of air, although the largest quantities were most frequently detected in the air of the building for post-slaughter waste pre-treatment in chemical treatment plant.
Źródło:
Journal of Ecological Engineering; 2016, 17, 5; 17-21
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Maillard neoglycans as inhibitors for in vitro adhesion of F4+ enterotoxigenic Escherichia coli to piglet intestinal cells
Autorzy:
Sarabia-Sainz, Héctor
Mata Haro, Verónica
Sarabia Sainz, José
Vázquez-Moreno, Luz
Montfort, Gabriela
Powiązania:
https://bibliotekanauki.pl/articles/1038559.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Albumin glycation
biorecognition
anti-adhesion
E. coli
Opis:
Adhesion of enterotoxigenic (ETEC) E. coli to host intestinal cells is mediated by lectin-like fimbriae that bind to specific glycan moieties on the surfaces of enterocytes. To prevent in vitro binding of E. coli F4 fimbriae (F4 ETEC+) to piglet enterocytes, neoglycans were synthesized by the Maillard reaction conjugating lactose (Lac), galacto-oligosaccharides (GOS) or chitin oligosaccharides (Ochit) to porcine serum albumin (PSA). Neoglycans were characterized by SDS-PAGE, intrinsic tryptophan fluorescence and recognition by plant lectins, as well as by F4 ETEC variants. Electrophoretic patterns suggested the binding to PSA of 63, 13 and 2 molecules of Lac, GOS and Ochit, respectively. All neoglycans displayed quenching of tryptophan fluorescence consistent with the degree of glycation estimated by SDS-PAGE. Plant lectins recognized the neoglycans according to their specificity, whereas antigenic variants of F4 ETEC (ab, ac and ad) recognized PSA-Ochit and PSA-Lac with higher affinity than that for GOS. Neoglycans partially hindered the in vitro binding of F4+ ETEC to piglet enterocytes in a dose-dependent manner. The most effective blocking was observed with PSA-Lac that partially inhibited the adhesion of bacteria to enterocytes in a dose dependent manner, as quantified by flow cytometry. Increased production of the cytokines IL-6 and TNF-α was observed in response to F4+ ETEC infection of enterocytes and production was reduced in the presence of PSA-Ochit and PSA-GOS. These results suggest that neoglycans synthesized by the Maillard reaction could be useful in the prophylaxis of diarrhea in piglets.
Źródło:
Acta Biochimica Polonica; 2017, 64, 4; 679-686
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Substrate selection by RNA polymerase from E. coli. The role of ribose and 5-triphosphate fragments, and nuclcotides interaction
Autorzy:
Szafranski, Przemysław
Smagowicz, Wiesław
Wierzchowisk, Kazimierz
Powiązania:
https://bibliotekanauki.pl/articles/1045974.pdf
Data publikacji:
1985
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1985, 32, 4; 329-349
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation of bacteria from soil sample of Tamil Nadu and their in vitro interaction
Autorzy:
Sankari, D.
Khusro, Ameer
Aarti, Chirom
Powiązania:
https://bibliotekanauki.pl/articles/1031683.pdf
Data publikacji:
2020
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antagonism
B. subtilis
CFU
E. coli
Interaction
P. aeruginosa
Opis:
The present study was investigated to determine the competitive interaction between Gram positive (B. subtilis) and Gram negative (P. aeruginosa and E. coli) bacteria in vitro. Bacteria of interest were isolated from soil sample and were identified through morphological and biochemical tests. Bacterial cultures were grown alone and as co- culture in test tubes containing sterile broth. Bacterial growth and their growth inhibition in co- culture tubes were analyzed till 96 h through spectrophotometric assay and colony forming unit (CFU) assay. Spectrophotometric analysis and CFU/mL assay showed that the effect of B. subtilis on P. aeruginosa and E. coli in co- culture tubes was growth inhibitory. This is accompanied by the reduction in absorbance value and CFU/mL of the co-culture tubes. The bactericidal activity of B. subtilis was measured on P. aeruginosa and E. coli using agar well diffusion method. B. subtilis showed zone of inhibition of 8 mm and 6 mm against P. aeruginosa and E. coli, respectively. The antagonistic activity test of B. subtilis favoured the spectrophotometric and colony forming assay results. These results suggest that B. subtilis develop adaptational pathways by extracellular signaling molecules and antibacterial factors in order to compete with bacterial neighbors.
Źródło:
World News of Natural Sciences; 2020, 28; 67-75
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Escherichia coli in sewage sludge - detection method
Escherichia coli w osadach ściekowych - metoda wykrywania
Autorzy:
Machnicka, A.
Powiązania:
https://bibliotekanauki.pl/articles/106338.pdf
Data publikacji:
2014
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
E.coli
sewage sludge
enrichment of Escherichia coli
detection of Escherichia coli
E. coli
osady ściekowe
namnażanie Escherichia coli
wykrywanie Escherichia coli
Opis:
Escherichia coli is Gram-negative optionally anaerobic roads which belongs to Enterobacteriaceae family. Includes in a physiological bacterial flora of human and warm-blooded animals large intestine. Escherichia coli is being met in abiotic elements of the environment so as waters, wastewater, sewage sludge, soil and the food. This bacterium is showing the pathogenicity in named terms for the peoples, triggering diseases mainly: gastrointestinal tract and urinary. Quality and quantitative proposed detections method of the bacteria E. coli contains five/six steps: - appointment dry suspended solid, - preparation averaged, test of sample and resuscitation of bacteria, - making dilutions, - enrichment and differentiation in chromogenic-selective medium, - enumerating the amount of cfu E. coli in 1 g of a dry weight, - optionally, the biochemical identification.
Escherichia coli jest Gram-negatywną, względnie beztlenową pałeczką należącą do rodziny Enterobacteriaceae. Wchodzi w skład fizjologicznej flory bakteryjnej jelita grubego człowieka oraz zwierząt stałocieplnych. Spotyka się ją w elementach abiotycznych środowiska, takich jak wody, ścieki, osady ściekowe, gleba i żywność. Bakteria ta w określonych warunkach wykazuje chorobotwórczość dla człowieka, wywołując głównie schorzenia układów pokarmowego i moczowego. Proponowana metoda jakościowa i ilościowa wykrywania bakterii E. coli zawiera pięć/sześć etapów: - oznaczenie suchej masy osadu, - przygotowanie próbki uśrednionej i badawczej oraz przywrócenie bakteriom aktywności fizjologicznej, - wykonanie rozcieńczeń, - namnażanie i identyfikacja na podłożu chromogennym-selektywnym, - wyliczenie ilości jtk E. coli w 1 g suchej masy osadu, - opcjonalnie identyfikacja biochemiczna.
Źródło:
Chemistry-Didactics-Ecology-Metrology; 2014, 19, 1-2; 79-85
2084-4506
Pojawia się w:
Chemistry-Didactics-Ecology-Metrology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Screening for ESβL producing E. coli isolated from clinical urine samples collected from different places of Kalaburagi city
Autorzy:
Ramachar, Raghavendra
Gaddad, Subhashchandra M.
Shivannavar, Channappa T.
Powiązania:
https://bibliotekanauki.pl/articles/1177430.pdf
Data publikacji:
2018
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antibiogram
Double-disc diffusion test
ESβL
Green Metallic Sheen
Phenotypic detection
Uropathogenic E. coli
Opis:
Bacteria are capable of invading and infecting humans, leading to disease and sometimes death. Different body tissues, organs and systems are vulnerable to different organisms. This study was aimed to isolate and identify the bacteria causing Urinary Tract Infection (UTI) in different age groups and also screened for ESβL production. The mid-stream urine samples showing symptoms of urinary tract infections were collected in a wide container from different hospitals and diagnostic centres of Kalaburagi city. The isolation of uropathogen was done by semi-quantitative method of inoculating the samples on the selective and differential media such as Eosin Methylene Blue (EMB) and MacConkey (MAC) agar media respectively. The isolated pathogen was identified by conventional methods like cultural, morphological and biochemical tests. The antibiotic susceptibility test was carried out by Kirby-Bauer disc diffusion technique and ESβL production by double disk-diffusion test (DDDT) as per CLSI guidelines (2013). Out of 550 samples screened, a total of 288 bacteria were isolated of those half of them (146) were E. coli isolates and all were found to be multidrug resistant and half of them (50%) were of ESβL producers. All ESβL producing E. coli isolates were resistant to ceftazidime and exhibited higher level of resistance to Cephalothin, Erythromycin, Cotrimoxazole and Aztreonam. As ESβL producing organisms limits the available treatment options, so, the timely administration of sensitive antibiotic and avoiding antibiotic abuse will help in curing the disease without going for the costly drugs such as Carbapenems. Our study revealed that nitrofurantoin, ofloxacin and cefoxitin may be considered as drug of choice for the treatment of UTI patients. The ESβL production in uropathogens should be continuously monitored in the clinics and hospitals as to avoid the emergence of more multi drug resistance strains.
Źródło:
World Scientific News; 2018, 103; 107-118
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plant-derived rhamnogalacturonan-I’s modulate proinflammatory cytokine gene expression in neutrophils stimulated by E. coli LPS and P. gingivalis bacteria
Autorzy:
Folkert, J.
Mieszkowska, A.
Burke, B.
Addison, O.
Gurzawska, K.
Powiązania:
https://bibliotekanauki.pl/articles/285453.pdf
Data publikacji:
2018
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
pectin
PMN
proinflammatory cytokines
gene expression
Opis:
Titanium dental implants often induce the foreign body immune response. The duration of the inflammatory process determines the initial stability and biocompatibility of the implant. The challenge for bone tissue engineering is to develop implant biocompatible and bioactive surface coatings that regulate the inflammatory response and enhance osseointegration. Pectins, plant-derived polysaccharides, have been shown to be potential candidates for surface coating due to their possible roles in improving osseointegration and bone healing. The aim of this study was to evaluate in vitro the effect of plant-derived pectin rhamnogalacturonan-I (RG-I) nanocoating on pro- and anti-inflammatory human polymorphonuclear leucocytes (PMN) responses to E. coli LPS or P. gingivalis bacteria. In this study unmodified RG-I and structurally modified RG-I from potato were examined. All in vitro studies were performed on tissue culture polystyrene surfaces (TCPS) or titanium (Ti) discs coated with unmodified and modified RG-Is. Changes in PMN gene expression occurred on both surfaces. The presence of RG-Is down-regulated proinflammatory genes, IL1B, IL8, TNFA. Our results clearly showed that pectin RG-I nanocoating decreased the level of proinflammatory genes expression in stimulated PMN and may therefore be considered as a potential candidate for modulation of the inflammatory response elicited by insertion of implants into living tissue.
Źródło:
Engineering of Biomaterials; 2018, 21, 144; 2-7
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mutator specificity of Escherichia coli alkB117 allele
Autorzy:
Nieminuszczy, Jadwiga
Janion, Celina
Grzesiuk, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1041261.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
lacZ → Lac+ reversion
mutational specificity
E. coli
MMS
alkB117
Opis:
The Escherichia coli AlkB protein encoded by alkB gene was recently found to repair cytotoxic DNA lesions 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) by using a novel iron-catalysed oxidative demethylation mechanism that protects the cell from the toxic effects of methylating agents. Mutation in alkB results in increased sensitivity to MMS and elevated level of MMS-induced mutations. The aim of this study was to analyse the mutational specificity of alkB117 in a system developed by J.H. Miller involving two sets of E. coli lacZ mutants, CC101-106 allowing the identification of base pair substitutions, and CC107-CC111 indicating frameshift mutations. Of the six possible base substitutions, the presence of alkB117 allele led to an increased level of GC→AT transitions and GC→TA and AT→TA transversions. After MMS treatment the level of GC→AT transitions increased the most, 22-fold. Among frameshift mutations, the most numerous were -2CG, -1G, and -1A deletions and +1G insertion. MMS treatment appreciably increased all of the above types of frameshifts, with additional appearance of the +1A insertion.
Źródło:
Acta Biochimica Polonica; 2006, 53, 2; 425-428
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
COMPARATIVE IN VITRO STUDIES OF FURAZIDIN AND NITROFURANTOIN ACTIVITIES AGAINST COMMON UROPATHOGENS INCLUDING MULTIDRUG-RESISTANT STRAINS OF E. COLI AND S. AUREUS
Autorzy:
Klesiewicz, Karolina
Karczewska, Elżbieta
Nowak, Paweł
Mrowiec, Paulina M.
Skiba-Kurek, Iwona
Białecka, Joanna
Majka, Zbigniew
Berdzik-Kalarus, Sylwia
Budak, Alicja
Zajdel, Paweł
Powiązania:
https://bibliotekanauki.pl/articles/895689.pdf
Data publikacji:
2018-06-30
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
ESBL-positive E. coli
MRSA
furazidin
nitrofurantoin
urinary tract infections
Opis:
Abstract: Urinary tract infections caused by wide range of pathogens including gram-negative and gram-positive bacteria as well as fungi are a severe public health problem. The predominant causative agent of both uncomplicated and complicated urinary tract infections is Escherichia coli. In an era of increasing bacterial resistance to antimicrobial agents and a high prevalence of multidrug-resistant (MDR) strains in community and hospital acquired infections, the re-evaluation of older generations of antimicrobial agents, such as nitrofuran derivatives, seems to be a reasonable approach. The aim of the study was to evaluate furazidin activity against common uropathogens in comparison to nitrofurantoin and other selected antimicrobial agents, routinely used in the treatment of urinary tract infections. Furazidin exhibited lower MICs than nitrofurantoin when tested against gram-negative and gram-positive bacteria including clinical MDR E. coli and methicillin-resistant Staphylococcus aureus. The MICs for furazidin ranged from 4 to 64 mg/L for Enterobacteriaceae strains, from 2 to 4 mg/L for gram-positive cocci, and 0.5 mg/L for anaerobic bacteria. The MICs for nitrofurantoin ranged from 16 to 64 mg/L for Enterobacteriaceae strains, from 8 to 64 mg/L for gram-positive cocci, and 4 mg/L for anaerobic bacteria. In addition, both nitrofurans displayed better activity against the tested bacterial strains than ciprofloxacin, fosfomycin, trimethoprim and co-trimoxazole. Nitrofuran derivatives displayed higher antimicrobial activity than other antimicrobial agents regardless of bacteria species or resistance mechanism.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 3
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Działanie pałeczki okrężnicy typu 0 111:B4 na makroorganizm
The action of E.coli 0 111:B4 on the macroorganism
Autorzy:
Zaleski, S.
Powiązania:
https://bibliotekanauki.pl/articles/871334.pdf
Data publikacji:
1960
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Źródło:
Roczniki Państwowego Zakładu Higieny; 1960, 11, 4
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
CEA-negative glioblastoma and melanoma cells are sensitive to cytosine deaminase/5-fluorocytosine therapy directed by the carcinoembryonic antigen promoter.
Autorzy:
Dąbrowska, Anna
Szary, Jarosław
Kowalczuk, Małgorzata
Szala, Stanisław
Ugorski, Maciej
Powiązania:
https://bibliotekanauki.pl/articles/1041550.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
CEA promoter
E. coli cytosine deaminase
gene-directed enzyme prodrug therapy
Opis:
Recent studies have suggested that carcinoembryonic antigen (CEA)-promoter sequences are active only in CEA-positive cells, filing in the criteria for tumor specific targeting of suicide genes. However, the present study on gene therapy of colon cancer and cell-specificity of CEA promoter, provide evidence that CEA-positive and CEA-negative cells transfected with E. coli cytosine deaminase (CD) gene under the control of CEA promotor sequence are sensitive to enzyme/pro-drug therapy with 5-fluorocytosine (5-FC). Individual clones derived from the CEA-negative cell lines: melanoma Hs294T and glioblastoma T98G after transfection with CD differed profoundly in their sensitivity to 5-FC. The IC50 values for several clones of the CEA-negative cells were almost the same as for CEA-positive colon cancer cells. Such 5-FC-sensitive clones derived from the population of CEA-negative cells, present even in small number, because of the very effective bystender effect of this enzyme/pro-drug system can cause severe problems during therapy by efficiently killing surrounding normal cells. Safety is the major issue in gene therapy. Our data suggest that the safety of gene-directed enzyme pro-drug therapy (GDEPT) with CEA promoter driven expression of therapeutic genes is not so obvious as it has originally been claimed.
Źródło:
Acta Biochimica Polonica; 2004, 51, 3; 723-732
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection and enumeration of Enteric bacteria associated with food handlers and surfaces of food manufacturing industry located in Hub city, Pakistan
Autorzy:
Ali, Razim
Hayat, Amir
Fatima, Madiha
Noman, Muhammad
Powiązania:
https://bibliotekanauki.pl/articles/1182928.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
enterobacteriaceae
foodborne microbes
hygiene
surface contamination
food handlers
e. coli
sanitation
Opis:
Foodborne microorganisms harbor and adheres itself to the food material and surrounding surfaces for a long time and influence the food quality and consumers health. Among these microbes the presence of Enteric indicator bacteria in food premises confers the indication of entero-pathogens, i.e. E. coli, Salmonella, Shigella and Campylobacters that could cause severe systemic infections in consumers. In this study, the hygienic status of confectionery and supplementary food processing facility was evaluated. A total of 10497 examinations were performed on 3499 swab samples collected from food premises and handlers for the analysis of Enteric indicator bacteria. From swabs, 1277 (12.2%) isolates were identified in which Enterobacteriaceae were found with higher frequency 604 (47.3%) followed by Coliforms 293 (30.8%) and Escherichia coli 280 (21.9%) respectively. The mean count (CFU/cm2) was found maximum for plain surfaces (floors, walls and door), while the lowest was for equipment and machinery. Overall isolates percent prevalence was determined where Enterobacteriaceae were 47%, Coliforms 31% and Escherichia coli 22%. Majority of the floor surfaces were highly contaminated, where washing and sanitation practices were observed to be inappropriate. Worker hygiene status was lacking essential food safety and hygiene standards. In general, the Enteric bacteria were found with higher ratio, that could affect the food quality and quantity both to a greater extent with some influences on consumers health.
Źródło:
World Scientific News; 2016, 49, 2; 192-203
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Limited proteolysis of E. coli ATP-dependent protease Lon - a unified view of the subunit architecture and characterization of isolated enzyme fragments
Autorzy:
Melnikov, Edward
Andrianova, Anna
Morozkin, Andrey
Stepnov, Anton
Makhovskaya, Oksana
Botos, Istvan
Gustchina, Alla
Wlodawer, Alexander
Rotanova, Tatyana
Powiązania:
https://bibliotekanauki.pl/articles/1040741.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
AAA+ protein
ATP-dependent proteases
Lon
Lon domains
Limited proteolysis
Opis:
We carried out chymotryptic digestion of multimeric ATP-dependent Lon protease from Escherichia coli. Four regions sensitive to proteolytic digestion were located in the enzyme and several fragments corresponding to the individual structural domains of the enzyme or their combinations were isolated. It was shown that (i) unlike the known AAA+ proteins, the ATPase fragment (A) of Lon has no ATPase activity in spite of its ability to bind nucleotides, and it is monomeric in solution regardless of the presence of any effectors; (ii) the monomeric proteolytic domain (P) does not display proteolytic activity; (iii) in contrast to the inactive counterparts, the AP fragment is an oligomer and exhibits both the ATPase and proteolytic activities. However, unlike the full-length Lon, its AP fragment oligomerizes into a dimer or a tetramer only, exhibits the properties of a non-processive protease, and undergoes self-degradation upon ATP hydrolysis. These results reveal the crucial role played by the non-catalytic N fragment of Lon (including its coiled-coil region), as well as the contribution of individual domains to creation of the quaternary structure of the full-length enzyme, empowering its function as a processive protease.
Źródło:
Acta Biochimica Polonica; 2008, 55, 2; 281-296
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of SOS-induced Pol II, Pol IV, and Pol V DNA polymerases on UV-induced mutagenesis and MFD repair in Escherichia coli cells.
Autorzy:
Wrzesiński, Michał
Nowosielska, Anetta
Nieminuszczy, Jadwiga
Grzesiuk, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1041471.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
MFD
polB
UV induced mutagenesis
E. coli
dinB
mutD5
umuDC
Opis:
Irradiation of organisms with UV light produces genotoxic and mutagenic lesions in DNA. Replication through these lesions (translesion DNA synthesis, TSL) in Escherichia coli requires polymerase V (Pol V) and polymerase III (Pol III) holoenzyme. However, some evidence indicates that in the absence of Pol V, and with Pol III inactivated in its proofreading activity by the mutD5 mutation, efficient TSL takes place. The aim of this work was to estimate the involvement of SOS-inducible DNA polymerases, Pol II, Pol IV and Pol V, in UV mutagenesis and in mutation frequency decline (MFD), a mechanism of repair of UV-induced damage to DNA under conditions of arrested protein synthesis. Using the argE3→Arg+ reversion to prototrophy system in E. coli AB1157, we found that the umuDC-encoded Pol V is the only SOS-inducible polymerase required for UV mutagenesis, since in its absence the level of Arg+ revertants is extremely low and independent of Pol II and/or Pol IV. The low level of UV-induced Arg+ revertants observed in the AB1157mutD5ΔumuDC strain indicates that under conditions of disturbed proofreading activity of Pol III and lack of Pol V, UV-induced lesions are bypassed without inducing mutations. The presented results also indicate that Pol V may provide substrates for MFD repair; moreover, we suggest that only those DNA lesions which result from umuDC-directed UV mutagenesis are subject to MFD repair.
Źródło:
Acta Biochimica Polonica; 2005, 52, 1; 139-147
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Using multitype branching models to analyze bacterial pathogenicity
Autorzy:
Tahir, Daniah
Kaj, Ingemar
Bartoszek, Krzysztof
Majchrzak, Marta
Parniewski, Pawel
Sakowski, Sebastian
Powiązania:
https://bibliotekanauki.pl/articles/747904.pdf
Data publikacji:
2020
Wydawca:
Polskie Towarzystwo Matematyczne
Tematy:
Markov models
branching processes
limit theorems
virulence factors
E. coli strains
Opis:
We apply multitype, continuous time, Markov branching models to study pathogenicity in E. coli, a bacterium belonging to the genus Escherichia. First, we examine briefly, the properties of multitype branching processes and we also survey some fundamental limit theorems regarding the behavior of such models under various conditions. These theorems are then applied to discrete, state dependent models, in order to analyze pathogenicity in a published clinical data set consisting of 251 strains of E. coli. We use well established methods, incorporating maximum likelihood techniques, to estimate speciation rates as well as the rates of transition between different states of the models. From the analysis, we not only derive new results, but we also verify some preexisting notions about virulent behavior in bacterial strains.
Źródło:
Mathematica Applicanda; 2020, 48
1730-2668
2299-4009
Pojawia się w:
Mathematica Applicanda
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Public health hazards in Poland posed by foodstuffs contaminated with E.Coli O104:H4 bacterium from the recent European out break
Autorzy:
Bilinski, P.
Kapka-Skrzypczak, L.
Posobkiewicz, M.
Bondaryk, M.
Holownia, P.
Wojtyla, A.
Powiązania:
https://bibliotekanauki.pl/articles/52231.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Opis:
Shiga toxin producing Escherichia coli (STEC) are the most virulent diarrhoeagenic E. coli known to date. They can spread with alarming ease via the food chain, as recently demonstrated by the large outbreak of STEC O104:H4 borne by sprouted seeds in 2011, clustered in northern Germany, and subsequently affecting other countries. Indeed, a significant number of infections to verocytotoxin producing Escherichia coli O104:H4 have been reported from the WHO European Region resulting in many cases of bloody diarrhoea and haemolytic uraemic syndrome in Germany, 15 other European countries and North America. Eventually, the European Food Standards Agency, (EFSA), identified the likely source to a single consignment of fenugreek seeds from an Egyptian exporter as being linked to the two outbreaks in Germany and France. The situation was closely monitored by the Chief Sanitary Inspectorate public health authority in Poland where actions undertaken ensured that the public was well informed about the dangers of STEC contamination of food, how to avoid infection, and what to do if infected. Tracing the fenugreek distributors also enabled the identification of suspected batches and their isolation. As a result, there were very few reported cases of STEC infection in Poland. Effective control over such outbreaks is therefore a vital public health task. This should include early detection and rapid identification of the contagion mode, followed by removing the foodstuff(s) from the market, providing consumer advice, and preventing secondary spreading. As a mitigation measure, screening/monitoring those involved in food handling is also warranted to exclude carriers who can be asymptomatic.
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial recognition of thermal glycation products derived from porcine serum albumin with lactose
Autorzy:
Sarabia-Sainz, Andre-i
Ramos-Clamont, Gabriela
Winzerling, Joy
Vázquez-Moreno, Luz
Powiązania:
https://bibliotekanauki.pl/articles/1039957.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
neoglycans
bacterial recognition
E. coli K88 adhesion
glycation of serum albumin
Opis:
Recently, glyco-therapy is proposed to prevent the interaction of bacterial lectins with host ligands (glycoconjugates). This interaction represents the first step in infection. Neoglycans referred to as PSA-Lac (PSA-Glu (β1-4) Gal) were obtained by conjugation of porcine serum albumin (PSA) with lactose at 80 °C, 100 °C and 120 ºC. Characterization studies of the products showed that PSA could contain 1, 38 or 41 added lactoses, depending on the reaction temperature. These neoglycans were approximately 10 times more glycated than PSA-Lac obtained in previous work. Lactose conjugation occurred only at lysines and PSA-Lac contained terminal galactoses as confirmed by Ricinus communis lectin recognition. Furthermore, Escherichia coli K88+, K88ab, K88ac and K88ad adhesins showed affinity toward all PSA-Lac neoglycans, and the most effective was the PSA-Lac obtained after 100 ºC treatment. In vitro, this neoglycan partially inhibited the adhesion of E. coli K88+ to piglet mucin (its natural ligand). These results provide support for the hypothesis that glycated proteins can be used as an alternative for bioactive compounds for disease prevention.
Źródło:
Acta Biochimica Polonica; 2011, 58, 1; 95-100
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of microbiological quality of water in the Nowohucki Reservoir with particular regard to microorganisms potentially dangerous to humans
Autorzy:
Wolny-Koładka, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/1035482.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
"E. coli"
"E. faecalis"
"Nowohucki Reservoir"
"mesophilic bacteria"
"microbiological contamination of water"
"surface water"
Opis:
Introduction. This study was aimed to assess the microbiological quality of water in the Nowohucki Reservoir (Kraków, Poland) as well as to determine whether its waters contain microorganisms potentially dangerous from an epidemiological point of view. Material and methods. Microbiological analyses included the determination of the number of mesophilic and psychrophilic bacteria, coliforms, fecal E. coli, as well as E. faecalis, C. perfringens, Staphylococcus spp. and Salmonella spp.. Water samples were collected 4 times per year on April 27th 2015 (spring), July 10th 2015 (summer), October 12th 2015 (autumn) and December 29th 2015 (winter) at 5 points within the area of the reservoir. Water and air temperature was measured onsite. Results. It was found that the prevalence of the analyzed microorganisms was affected by changing water and air temperature as well as by using this reservoir during holiday season for swimming purposes by local residents. All analyzed microbiological indicators of poor water quality were found in the analyzed water samples, which may pose a potential health risk to people swimming in the considered reservoir. Conclusions. From an epidemiological point of view, it is reasonable to include the Nowohucki Reservoir into a constant sanitary monitoring programme.
Wstęp. Badania miały na celu ocenę stanu mikrobiologicznego wód Zalewu w Nowej Hucie (Kraków, Polska) a także określenie, czy w zbiorniku występują drobnoustroje potencjalnie niebezpieczne z epidemiologicznego punktu widzenia. Materiał i metody. Analizy mikrobiologiczne obejmowały określenie liczebności bakterii mezofilnych, psychrofilnych, bakterii grupy coli, form kałowych E. coli, a także E. faecalis, C. perfringens, Staphylococcus spp. i Salmonella spp.. Próbki wód pobierane były 4 razy w roku w następujących terminach: 27.04.2015 r. (wiosna), 10.07.2015 r. (lato), 12.10.2015 r. (jesień) i 29.12.2015 r. (zima) w 5 punktach na terenie zalewu. Ponadto, w każdym punkcie pomiarowym mierzono temperaturę wody oraz powietrza. Wyniki. Stwierdzono, że na liczebność badanych drobnoustrojów wpływała zmieniająca się temperatura wody i powietrza oraz użytkowanie w sezonie wakacyjnym zalewu w charakterze kąpieliska przez okolicznych mieszkańców. W pobranych próbkach zidentyfikowano wszystkie badane wskaźniki czystości mikrobiologicznej wód, które stanowią potencjalne zagrożenie dla kąpiących się w zbiorniku ludzi. Wnioski. Z epidemiologicznego punktu widzenia zasadnym jest objęcie wód Zalewu w Nowej Hucie stałym monitoringiem sanitarnym.
Źródło:
Medycyna Środowiskowa - Environmental Medicine; 2016, 19, 4; 19-26
1505-7054
2084-6312
Pojawia się w:
Medycyna Środowiskowa - Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reverse osmosis as one-step wastewater treatment: a case study on groundwater pollution
Autorzy:
Ali, W.
Rehman, W. U.
Younas, M.
Ahmad, M. I.
Gul, S.
Powiązania:
https://bibliotekanauki.pl/articles/779416.pdf
Data publikacji:
2015
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
reverse osmosis (RO)
membrane separation
Total Dissolved Solids (TDS)
Total Coliform
E. Coli
Opis:
The present case study is focused on performance evaluation of a Reverse Osmosis (RO) membrane based pilot plant to treat the ground/surface drinking water sources polluted by flood, rivers and/or canals. The RO plant was designed, fabricated and then operated with polluted water. Two feed water samples having a TDS of 2.000 mg · L−1 and 10.000 mg · L−1 respectively, were taken and analyzed for physical, chemical and microbiological contaminants. The RO plant was run once through over a span of 100 hrs at an operating pressure of 15 bar. TDS rejections were found to be more than 94% while permeate flux was measured to be 25.82 L · m−2 · hr−1 to 40.55 L · m−2 · hr−1. Results show that RO plant has a potential to remove physical, chemical and microbiological contaminants like Total Coliform and E. Coli in one step.
Źródło:
Polish Journal of Chemical Technology; 2015, 17, 4; 42-48
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Design of small molecule inhibitors of type III secretion system ATPase EscN from enteropathogenic Escherichia coli
Autorzy:
Bzdzion, Lukasz
Krezel, Hanna
Wrzeszcz, Karol
Grzegorek, Irmina
Nowinska, Katarzyna
Chodaczek, Grzegorz
Swietnicki, Wieslaw
Powiązania:
https://bibliotekanauki.pl/articles/1038684.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Escherichia coli (E. coli)
type III secretion system (T3SS)
enzyme inhibitor
small molecule
Opis:
Enteropathogenic E. coli (EPEC) is a human pathogen using type III secretion system for delivery of proteins directly into the human host. The system contains a single ATPase, EscN, which is essential for uncoupling of proteins from their complexes with chaperones before the delivery. The structure of EscN ATPase (PDB code: 2obm) was used to screen computationally for small molecule inhibitors blocking its active site. Two lead candidates were examined but only one, Compound 54, was selected for further optimization. After extended QSAR optimization, two derivatives were found to be competitive inhibitors of EscN capable of blocking ATPase activity with a Ki below 50 µM. One candidate, WEN05-03, with a Ki=16±2 µM, was also minimally toxic to mammalian cells as determined by other assays. In the cell infection model of HeLa cells with EPEC, Compound WEN05-03 completely blocked actin cluster formation at 100 µM concentration, when analyzed by confocal microscopy. The second best inhibitor of EscN ATPase activity was WEN04-34 with a Ki=46±2 µM. However, the compound was highly toxic to the BALB/3T3 cell line. In summary, the work identifies a compound blocking bacterial ATPase in its active site without causing cellular toxicity to the host cells. It is the first report showing feasibility of using bacterial virulence system ATPase as a target for safe, non-toxic compounds and offering a proof-of-concept for non-antibiotic alternatives.
Źródło:
Acta Biochimica Polonica; 2017, 64, 1; 49-63
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The epidermal growth factor-like domain from tissue plasminogen activator. Cloning in E. coli, purification and ESR studies of its interaction with human blood platelets
Autorzy:
Pietrucha, Tadeusz
Stec, Wojciech
Okruszek, Andrzej
Uznański, Bogdan
Koziołkiewicz, Maria
Wilk, Andrzej
Płucienniczak, Andrzej
Świątkowska, Maria
Cierniewski, Czesław
Powiązania:
https://bibliotekanauki.pl/articles/1045415.pdf
Data publikacji:
1994
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1994, 41, 1; 25-34
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Spontaneous mutagenesis in exponentially growing and stationary-phase, umuDC-proficient and -deficient, Escherichia coli dnaQ49.
Autorzy:
Nowosielska, Anetta
Nieminuszczy, Jadwiga
Grzesiuk, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1041546.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
dnaQ49
argE3→Arg+ revertants
E. coli
Pol V
spontaneous mutagenesis
stationary-phase mutations
Opis:
Spontaneous mutations arise not only in exponentially growing bacteria but also in non-dividing or slowly dividing stationary-phase cells. In the latter case mutations are called adaptive or stationary-phase mutations. High spontaneous mutability has been observed in temperature sensitive Escherichia coli dnaQ49 strain deficient in 3'→5' proofreading activity assured by the ε subunit of the main replicative polymerase, Pol III. The aim of this study was to evaluate the effects of the dnaQ49 mutation and deletion of the umuDC operon encoding polymerase V (Pol V) on spontaneous mutagenesis in growing and stationary-phase E. coli cells. Using the argE3OC →Arg+ reversion system in the AB1157 strain, we found that the level of growth-dependent and stationary-phase Arg+ revertants was significantly increased in the dnaQ49 mutant at the non-permissive temperature of 37°C. At this temperature, in contrast to cultures grown at 28°C, SOS functions were dramatically increased. Deletion of the umuDC operon in the dnaQ49 strain led to a 10-fold decrease in the level of Arg+ revertants in cultures grown at 37°C and only to a 2-fold decrease in cultures grown at 28°C. Furthermore, in stationary-phase cultures Pol V influenced spontaneous mutagenesis to a much lesser extent than in growing cultures. Our results indicate that the level of Pol III desintegration, dependent on the temperature of incubation, is more critical for spontaneous mutagenesis in stationary-phase dnaQ49 cells than the presence or absence of Pol V.
Źródło:
Acta Biochimica Polonica; 2004, 51, 3; 683-692
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis of some Novel Halogenated Platinum (II) Complexes of Active Schiff’s Base Ligand Derived from 5-Bromo Isatin and Evaluation of their Antibacterial Activity
Autorzy:
Patange, Ashok N.
Yadav, Uttam M.
Desai, Pratik A.
Singare, Pravin U.
Powiązania:
https://bibliotekanauki.pl/articles/1194025.pdf
Data publikacji:
2015
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
5-bromo Isatin
Bacillus pumilus
E. Coli
Micrococcus Lutes
Salmonella abony
Schiff’s base
Semicarbazone
Thiosemicarbazone
Opis:
This paper presents the synthesis and antibacterial studies some novel halogenated Platinum (II) complexes of active Schiff’s base ligands of the type [Pt(L1H)2]Cl2, [Pt(L2H)2]Cl2 and[Pt(L)2] derived from 5-Bromo Isatin. Where L1H= hydrazinecarbothiamide of 5-bromoindolinedione and L2H = hydrazinecarboxamide of 5-bromoindolinedione. All complexes reported here had been characterised by 1H NMR and IR spectral studies. Molar conductance studies support the 1:2 natures of these complexes. Pt (II) complexes are diamagnetic in nature and showing square planner geometry. The IR spectral data reveals that both the Schiff’s bases (L1H and L2H) behave as bidentate ligands and are co-ordinated to Pt (II) metal through the sulfur and hydrogenic nitrogen atom.all the new synthesized compounds were screened for antibacterial activity against four of the test organisms like Bacillus pumilus, Micrococcus Lutes, E. coli and Salmonella abony
Źródło:
World Scientific News; 2015, 10; 32-43
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Estimation of significance of AlkB and AlkA proteins in DNA repair in Escherichia coli model
Autorzy:
Sokołowska, B.
Maciejewska, A. M.
Jóźwik, A.
Kuśmierek, J. T.
Powiązania:
https://bibliotekanauki.pl/articles/333316.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Śląski. Wydział Informatyki i Nauki o Materiałach. Instytut Informatyki. Zakład Systemów Komputerowych
Tematy:
rozpoznawanie obrazów
klasyfikator najbliższych sąsiadów
analiza cech pracy odbiornika
naprawa DNA
adaptacyjna odpowiedź
powstawanie mutacji
bakterie e.coli
pattern recognition
fuzzy k-NN classifier
ROC analysis
DNA repair
adaptive response
mutagenesis
AlkB dioxygenase
AlkA glycosylase
E.coli
Opis:
The paper concerns estimation of significance of differences of mutagenesis level between the wild-type strain (wt) and its derivatives which differ in DNA repair ability, namely alkA and alkB strain, devoided AlkA glycosylase and AlkB dioxygenase activity, respectively. The strains were analyzed for their ability to repair 1,N6-ethenoadenine (εA) - chloroacetaldehyde adduct to DNA. The analysis was done using classical statistical and pattern recognition methods. The obtained results confirmed that AlkB dioxygenase plays the most important role in εA repair in E. coli in the experimental modeling.
Źródło:
Journal of Medical Informatics & Technologies; 2011, 17; 321-326
1642-6037
Pojawia się w:
Journal of Medical Informatics & Technologies
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Częstotliwość występowania niektórych serotypów E. coli w przypadkach znamiennej bakteriurii
Chastota nalichija nekotorykh serotipov v sluchajakh sushhestvennojj bakteriurii
The frequency of some Escherichia coli serotypes appearance in cases of significant bacteriuria
Autorzy:
Narbutowicz, B.
Wencel, J.
Szostakowska, M.
Hendrich, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2189858.pdf
Data publikacji:
1969
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1969, 05, 1-2; 83-86
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Effect of Garlic Extract Addition on Tilapia Skin Gelatin Based Edible Coating Towards Antimicrobial Properties and Fish Meatball’s Shelf Life
Autorzy:
Reynaldi, Ahmad
Rostini, Iis
Afrianto, Eddy
Rochima, Emma
Powiązania:
https://bibliotekanauki.pl/articles/1058047.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
E. coli
Pseudomonas aeruginosa
Salmonella typhimurium
Staphyllococcus aureus
TPC
edible coating
extract
garlic
pH values
shelf life
tilapia gelatin
Opis:
The purpose of this research is to determine the best concentration and effect of garlic extract incorporated on tilapia skin gelatin-based edible coating to preserve and extend the shelf life of fish meatball at cold storage. This research was conducted from May 2018 until October 2018 on Fisheries Product Processing Laboratory of The Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. The garlic extract conducted with maceration method with ethanol 96% was performed at Organic Chemical Laboratory FMIPA Universitas Padjadjaran, and for TPC test, inhibition test of edible coating Tilapia’s fish-skin with garlic extract was conducted in Microbiology and Molecular Biotechnology Laboratory of Faculty of Fisheries and Marine Sciences Universitas Padjadjaran. The method used is experimental method with 5 treatments which is meatballs without edible coating. The addition of garlic extract was done with various concentration at 0%, 1%, 1.5%, 2%. The observations were Total Plate Count (TPC) test, inhibition zone test against Pseudomonas aeruginosa, and pH value. The results showed that the best treatment is 2% garlic extract with TPC value 1.1×105 cfu/g during 14 days of storage and concluded that it could extend the shelf life of meatball until 14 days, pH value was 7.03 and inhibition zone against P. aeruginosa was 7.01 mm. The addition of garlic extract on tilapia skin gelatin-based edible coating could extend the shelf life of fish meatballs until 14 days on cold storage.
Źródło:
World Scientific News; 2019, 134, 2; 74-85
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-50 z 74

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