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Wyszukujesz frazę "1,3-propanediol" wg kryterium: Wszystkie pola


Tytuł:
Group II intron-mediated deletion of lactate dehydrogenase gene in an isolated 1,3-propanediol producer Hafnia alvei AD27
Autorzy:
Celińska, Ewelina
Drożdżyńska, Agnieszka
Wita, Agnieszka
Juzwa, Wojciech
Białas, Wojciech
Czaczyk, Katarzyna
Grajek, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1038697.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
1,3-propanediol
gene knock-out
metabolic engineering
Enterobacteriaceae
group II intron
lactate dehydrogenase
Opis:
Our previous studies showed that glycerol fermentation by Hafnia alvei AD27 strain was accompanied by formation of high quantities of lactate. The ultimate aim of this work was the elimination of excessive lactate production in the 1,3-propanediol producer cultures. Group II intron-mediated deletion of ldh (lactate dehydrogenase) gene in an environmental isolate of H. alvei AD27 strain was conducted. The effect of the Δldh genotype in H. alvei AD27 strain varied depending on the culture medium applied. Under lower initial glycerol concentration (20 gL-1), lactate and 1,3-propanediol production was fully abolished, and the main carbon flux was directed to ethanol synthesis. On the other hand, at higher initial glycerol concentrations (40 gL-1), 1,3-propanediol and lactate production was recovered in the recombinant strain. The final titers of 1,3-propanediol and ethanol were similar for the recombinant and the WT strains, while the Δldh genotype displayed significantly decreased lactate titer. The by-products profile was altered upon ldh gene deletion, while glycerol utilization and biomass accumulation remained unaltered. As indicated by flow-cytometry analyses, the internal pH was not different for the WT and the recombinant Δldh strains over the culture duration, however, the WT strain was characterized by higher redox potential.
Źródło:
Acta Biochimica Polonica; 2017, 64, 1; 123-133
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5
Autorzy:
Dąbrowski, Sławomir
Pietrewicz-Kubicz, Dorota
Zabłotna, Ewa
Długołęcka, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1039710.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
anaerobic fermentation
Clostridium butyricum
Escherichia coli
biodiesel
dha operon
glycerol
1,3-propanediol
Opis:
1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.
Źródło:
Acta Biochimica Polonica; 2012, 59, 3; 357-361
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Screening of environmental samples for bacteria producing 1,3-propanediol from glycerol
Autorzy:
Dąbrowski, Sławomir
Zabłotna, Ewa
Pietrewicz-Kubicz, Dorota
Długołęcka, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1039709.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
anaerobic fermentation
Clostridium
screening
biodiesel
glycerol
1,3-propanediol
Opis:
Twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. Seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. Seven of them produced 1,3-propanediol with a yield of 0.47-0.58. Six of the the isolated microorganisms were then classified as Clostridium butyricum (four strains), C. lituseburense (one strain), and C. sartagoforme (one strain). We suggest that of all these strains C. butyricum 2CR371.5 is the best 1,3-propanediol producer as producing no lactate as a by-product and growing well on a glycerol-containing medium.
Źródło:
Acta Biochimica Polonica; 2012, 59, 3; 353-356
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biotechnological production of 1,3-propanediol from crude glycerol
Autorzy:
Drozdzynska, A.
Leja, K.
Czaczyk, K.
Powiązania:
https://bibliotekanauki.pl/articles/81121.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
1,3-propanediol
bioconversion
biotechnological production
chemical industry
crude oil
glycerol
Klebsiella pneumoniae
metabolic pathway
microorganism
natural fat
waste biomass
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ budowy chemicznej na zmiany właściwości mechanicznych i termomechanicznych elastomerów biopoliuretanowych
The influence of chemical structure on mechanical and thermomechanical properties of cast biopolyurethane elastomers
Autorzy:
Głowińska, E.
Datta, J.
Włoch, M.
Różańska, M.
Powiązania:
https://bibliotekanauki.pl/articles/272694.pdf
Data publikacji:
2018
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Inżynierii Materiałów Polimerowych i Barwników
Tematy:
biopolyurethanes
bioglycols
bio-based 1,3-propanediol
bio-based 1,2-propanediol
biopoliuretany
bioglikole
bio-1,3-propanodiol
bio-1,2-propanodiol
Opis:
Przedmiotem niniejszej pracy są elastomery biopoliuretanowe otrzymywane metodą dwuetapową, tzw. prepolimerową, z wykorzystaniem surowców bio-pochodnych. Zbadano wpływ różnych małocząsteczkowych przedłużaczy łańcucha prepolimerowego: 1,2-biopropanodiolu oraz 1,3-biopropanodiolu na strukturę chemiczną i właściwości mechaniczne (warunki statyczne i dynamiczne) elastomerów biopoliuretanowych. Ponadto, wykorzystano również inny surowiec pochodzenia naturalnego – hydroksylowany olej sojowy (użyty jako składnik mieszaniny poliolowej). Za pomocą techniki FTIR-ATR stwierdzono zmiany w strukturze chemicznej wynikające ze zróżnicowania struktury chemicznej bioglikoli. Wyniki badań wytrzymałości na rozciąganie wskazują na korzyść z zastosowania bioglikoli zawierających w strukturze dwie równocenne pierwszorzędowe grupy hydroksylowe. Na podstawie badań analizy dynamiczno-mechanicznej (DMA) stwierdzono zmiany modułu zachowawczego (E′) i stratności (E″) oraz współczynnika stratności (tan δ).
The aim of the work was to obtain biopolyurethane elastomers from two bio-based components such as 1,2-biopropanediol or 1,3-biopropanediol (glycols) and hydroxylated soybean oil (as a mixture with commercial polyol). The influence of bio-based components on the chemical structure of elastomer and mechanical and thermo-mechanical elastomer properties (at static and dynamic conditions) were investigated. Based on FTIR-ATR results the changes in the chemical structure have been noticed, what is related to the diversified chemical structure of bioglycols and oil polyol, too. The results of static tensile tests show the benefits from using of bioglycol with two primary hydroxyl groups. Also the dynamic mechanical analysis (DMA) results indicated changes in the storage modulus (E′), loss modulus (E″) and damping factor (tan δ) values.
Źródło:
Elastomery; 2018, 22, 1; 40-50
1427-3519
Pojawia się w:
Elastomery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Studies of polypropylene membrane fouling during microfiltration of broth with Citrobacter freundii bacteria
Autorzy:
Gryta, M.
Waszak, M.
Tomaszewska, M.
Powiązania:
https://bibliotekanauki.pl/articles/779770.pdf
Data publikacji:
2015
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
microfiltration
fouling
glycerol fermentation
1,3-propanediol
Opis:
In this work a fouling study of polypropylene membranes used for microfiltration of glycerol solutions fermented by Citrobacter freundii bacteria was presented. The permeate free of C. freundii bacteria and having a turbidity in the range of 0.72–1.46 NTU was obtained. However, the initial permeate flux (100–110 L/m2h at 30 kPa of transmembrane pressure) was decreased 3–5 fold during 2–3 h of process duration. The performed scanning electron microscope observations confirmed that the filtered bacteria and suspensions present in the broth formed a cake layer on the membrane surface. A method of periodical module rinsing was used for restriction of the fouling influence on a flux decline. Rinsing with water removed most of the bacteria from the membrane surface, but did not permit to restore the initial permeate flux. It was confirmed that the irreversible fouling was dominated during broth filtration. The formed deposit was removed using a 1 wt% solution of sodium hydroxide as a rinsing solution.
Źródło:
Polish Journal of Chemical Technology; 2015, 17, 4; 56-64
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zatężanie roztworów 1,3-propanodiolu metodą destylacji membranowej
Concentration of 1,3-propanediol solutions by membrane distillation
Autorzy:
Gryta, M.
Powiązania:
https://bibliotekanauki.pl/articles/2072388.pdf
Data publikacji:
2013
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
destylacja membranowa
1,3-propanodiol
glicerol
zatężanie roztworów
membrane distillation
1,3-propanediol
glycerol
solution concentration
Opis:
Proces destylacji membranowej wykorzystano do zatężania roztworów 1,3-propanodiolu, uzyskując ponad 99,6% zatrzymanie alkoholu w koncentracie. W procesie zastosowano kapilarne membrany Accurel PP. Przeprowadzone długoterminowe badania zatężania (ponad 600 h) wykazały dobrą odporność użytych membran polipropylenowych na zwilżanie.
Membrane distillation process was utilized for the concentration of 1,3-propanediol solutions resulting in the retention of alcohol in retentate by more than 99.6%. Long-term studies of the concentration carried out for over 600 h had demonstrated a good resistance to wettability of used polypropy¬lene Accurel PP membranes.
Źródło:
Inżynieria i Aparatura Chemiczna; 2013, 5; 421--422
0368-0827
Pojawia się w:
Inżynieria i Aparatura Chemiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The study of glycerol-based fermentation and broth downstream by nanofiltration
Autorzy:
Gryta, Marek
Markowska-Szczupak, Agata
Grzechulska-Damszel, Joanna
Bastrzyk, Justyna
Waszak, Marta
Powiązania:
https://bibliotekanauki.pl/articles/778968.pdf
Data publikacji:
2014
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
glycerol fermentation
1,3-propanediol
nanofiltration
Citrobacter freundii
Opis:
In this work, the glycerol fermentation was carried out using Citrobacter freundii bacteria. The influence of glycerol and metabolites concentrations, and the pH changes on the efficiency of 1,3-propanediol production, during batch and fed-batch processes, was presented. The nanofiltration was used for the separation of obtained post-fermentation solutions. The resulted 1,3-PD solutions were significantly desalted, which may facilitate further downstream processes during 1,3-PD production.
Źródło:
Polish Journal of Chemical Technology; 2014, 16, 4; 117-122
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structure of Aqueous Solutions of 1,2- and 1,3-Propanediol Investigated by Positron Annihilation and Ultrasonic Methods
Autorzy:
Jerie, K.
Baranowski, A.
Gliński, J.
Orzechowski, K.
Powiązania:
https://bibliotekanauki.pl/articles/2008041.pdf
Data publikacji:
1999-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
61.25.Em
78.70.Bj
Opis:
The structure of aqueous solutions of 1,2-propanediol and 1,3-propane-diol was investigated using adiabatic compressibility measurements and positron annihilation methods. The experimental results are different than those obtained earlier for systems where hydrophobic hydration dominates. There are some evidences for increased rigidity of the water network, which can arise from the formation of hydrogen bonds between diols and water.
Źródło:
Acta Physica Polonica A; 1999, 95, 4; 562-568
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioconversion of crude glycerol to 1,3-Propanediol by immobilized cells of Citrobacter freundii
Autorzy:
Kowalska, Urszula
Mizielińska, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1179223.pdf
Data publikacji:
2017
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
1
3-PD
Citrobacter freundii
bioconversion
crude glycerol
immobilization
keramsite
Opis:
1,3-Propanediol (1,3-PD) was produced from crude glycerol through the fermentation by immobilized Citrobacter freundii cells. Microorganisms were immobilized on keramsite. and transferred to fermentation broth “M” with 50g/L glycerol. In parallel, conversion of glycerol was led in two bioreactors: column bioreactor for continuous production and standard bioreactor for static culture at the same conditions. The obtained results have shown that the use of immobilized cells of C. freundii reduced concentration of crude glycerol about 60%. The results have also demonstrated that immobilization was appropriate process for production of 1,3-PD in both continuous and static (bioreactor) conditions.
Źródło:
World Scientific News; 2017, 80; 18-28
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hypothetical glycerol pathways of newly isolated strains capable of 1,3-propanediol production
Autorzy:
Leja, Katarzyna
Samul, Dorota
Drożdżyńska, Agnieszka
Myszka, Kamila
Juzwa, Wojciech
Pawlicka, Joanna
Czaczyk, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/1039209.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Clostridium bifermentans
Clostridium butyricum
Hafnia alvei
1,3-propanediol
Opis:
Study presented here demonstrates the ability of three newly isolated strains, obtained from environmental probes (manure, bottom sediment, and food waste) and identified as Clostridium bifermentans, Clostridium butyricum, and Hafnia alvei, to synthesize 1,3-propanediol (1,3-PD), organic acids (such as lactic, acetic, fumaric, succinic, and butyric acids), and ethanol from glycerol. The production of 1,3-PD as well as the glycerol pathways in C. bifermentans and H. alvei cells have not been investigated and described yet by others. Moreover, there is no data in the available literature on the products of glycerol utilization by H. alvei and there is only some incoherent data (mainly from the first half of the twentieth century) about the ability of C. bifermentans to carry out glycerol degradation. Additionally, this study presents complete hypothetical glycerol pathways and the basic fermentation kinetic parameters (such as yield and productivity) for both strains as well as for the newly isolated C. butyricum strain.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 759-763
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
1,3-Propanediol production by Escherichia coli using genes from Citrobacter freundii atcc 8090
Autorzy:
Przystałowska, Hanna
Zeyland, Joanna
Kośmider, Alicja
Szalata, Marlena
Słomski, Ryszard
Lipiński, Daniel
Powiązania:
https://bibliotekanauki.pl/articles/1039012.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
bio-based 1,3-propanediol
genetically modified bacteria
glycerol utilization
renewable resources
Citrobacter freundii
Escherichia coli
Opis:
Compared with chemical synthesis, fermentation has the advantage of mass production at low cost, and has been used in the production of various industrial chemicals. As a valuable organic compound, 1,3-propanediol (1,3-PDO) has numerous applications in the production of polymers, lubricants, cosmetics and medicines. Here, conversion of glycerol (a renewable substrate and waste from biodiesel production) to 1,3-PDO by E. coli bacterial strain carrying altered glycerol metabolic pathway was investigated. Two gene constructs containing the 1,3-PDO operon from Citrobacter freundii (pCF1 and pCF2) were used to transform the bacteria. The pCF1 gene expression construct contained dhaBCE genes encoding the three subunits of glycerol dehydratase, dhaF encoding the large subunit of the glycerol dehydratase reactivation factor and dhaG encoding the small subunit of the glycerol dehydratase reactivating factor. The pCF2 gene expression construct contained the dhaT gene encoding the 1,3-propanediol dehydrogenase. Expression of the genes cloned in the above constructs was under regulation of the T7lac promoter. RT-PCR, SDS-PAGE analyses and functional tests confirmed that 1,3-PDO synthesis pathway genes were expressed at the RNA and protein levels, and worked flawlessly in the heterologous host. In a batch flask culture, in a short time applied just to identify the 1,3-PDO in a preliminary study, the recombinant E. coli bacteria produced 1.53 g/L of 1,3-PDO, using 21.2 g/L of glycerol in 72 h. In the Sartorius Biostat B Plus reactor, they produced 11.7 g/L of 1,3-PDO using 24.2 g/L of glycerol, attaining an efficiency of 0.58 [mol1,3-PDO/molglycerol].
Źródło:
Acta Biochimica Polonica; 2015, 62, 3; 589-597
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biotechnological conversion of glycerol from biofuels to 1,3-propanediol using Escherichia coli
Autorzy:
Przystałowska, Hanna
Lipiński, Daniel
Słomski, Ryszard
Powiązania:
https://bibliotekanauki.pl/articles/1039124.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
bioconversion
biodiesel
carbon source
Escherichia coli
glycerol
1,3-propanediol
Opis:
In the face of shortage of fossil fuel supplies and climate warming triggered by excessive carbon dioxide emission, alternative resources for chemical industry have gained considerable attention. Renewable resources and their derivatives are of particular interest. Glycerol, which constitutes one of the by-products during biodiesel production, is such a substrate. Thus, generated excess glycerol may become an environmental problem, since it cannot be disposed of in the environment. The most promising products obtained from glycerol are polyols, including 1,3-propanediol, an important substrate in the production of synthetic materials, e.g. polyurethanes, unsaturated polyesters, and epoxy resins. Glycerol can be used as a carbon and energy source for microbial growth in industrial microbiology to produce 1,3-propanediol. This paper is a review of metabolic pathways of native producers and E. coli with the acquired ability to produce the diol via genetic manipulations. Culture conditions during 1,3-PDO production and genetic modifications of E. coli used in order to increase efficiency of glycerol bioconversion are also described in this paper.
Źródło:
Acta Biochimica Polonica; 2015, 62, 1; 23-34
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Oczyszczanie 1,3-propanodiolu z brzeczki fermentacyjnej z zastosowaniem ekskluzji jonów
Separation of 1,3-propanediol from fermentation broth by ion exclusion
Autorzy:
Rukowicz, B
Miesiąc, I.
Alejski, K.
Powiązania:
https://bibliotekanauki.pl/articles/2072900.pdf
Data publikacji:
2014
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
1,3-propanodiol
brzeczka fermentacyjna
ekskluzja jonów
1,3-propanediol
fermentation broth
ion exclusion
Opis:
W pracy przedstawiono możliwość zastosowania ekskluzji jonów w procesie oczyszczania 1,3-propanodiolu z brzeczki fermentacyjnej. Badania przeprowadzono na kationicie w formie sodowej. Wstępnie odsoloną frakcję produktu uzyskano z ok. 50% wydajnością separacji 1,3-propanodiolu. Stopień odsolenia brzeczki wyniósł 80÷90%, a końcowe oczyszczenie i odbarwienie 1,3-propanodiolu przeprowadzono stosując wymianę jonową. W wyniku procesu otrzymano oczyszczony roztwór 1,3-propanodiolu o stężeniu 16,5 g/L.
Application of ion exclusion for the separation of 1,3-propanediol from fermentation broth was examined. The study was carried out on a cation exchange resin in the sodium form. The product fraction with the desalination degree of 80-90% and 50% separation yield of 1,3-propanediol we obtained. The final purification of 1,3-propanediol was carried out using the ion exchange. As a result of this process the purified solution of 1,3-propanediol (16.5 g/L) was obtained.
Źródło:
Inżynieria i Aparatura Chemiczna; 2014, 4; 288--289
0368-0827
Pojawia się w:
Inżynieria i Aparatura Chemiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Separation of 1,3-Propanediol from Aqueous Solutions by Ion Exchange Chromatography
Autorzy:
Rukowicz, B.
Miesiąc, I.
Alejski, K.
Powiązania:
https://bibliotekanauki.pl/articles/778477.pdf
Data publikacji:
2014
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
1,3-propanediol
glycerol
fermentation
sorption
ion-exchange chromatography
Opis:
1,3-propanediol is a promising monomer with many applications and can be produced by bioconversion of renewable resources. The separation of this product from fermentation broth is a difficult task. In this work, the application of cation exchange resin for the separation of 1,3-propanediol from model aqueous solution was examined. The best effect of separation of 1,3-propanediol from glycerol using sorption method was obtained for H+ resin form, although the observed partition coefficient of 1,3-propanediol was low. On the basis of the results of the sorption of 1,3-propanediol, the ionic forms of the resin were selected and used in the next experiments (H+, Ca2+, Ag+, Na+, Pb2+, Zn2+). The best results in ion exchange chromatography were obtained for cation exchange resin in H+ and Ca2+ form. The use of smaller particle size of resin and a longer length of the column allows to obtain better separation of mixtures.
Źródło:
Polish Journal of Chemical Technology; 2014, 16, 2; 82-86
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł

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