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Wyświetlanie 1-8 z 8
Tytuł:
Application of BAT-CELL Bio-Ambient Tests in exhaust gas emissions examinations for Euro 4 and Euro 6 combustion engines
Autorzy:
Keska, A.
Janicka, A.
Powiązania:
https://bibliotekanauki.pl/articles/99917.pdf
Data publikacji:
2017
Wydawca:
Wrocławska Rada Federacji Stowarzyszeń Naukowo-Technicznych
Tematy:
in vitro method
engine exhaust
Euro standards
Opis:
The introduction of the subsequent European emission standards raises controversy among vehicle manufacturers and researchers. In order to meet the legal requirements, it is necessary to continually improve not only the cars but also the costly measurement facilities. In addition, current methods do not give a clear answer to the harmful effects of exhaust gases on living organisms. By using the appropriate In vitro method to directly assess the toxicity of gaseous mixtures, one can obtain simple, reliable results that can be used to validate the introduction of ever more stringent Euro standards.
Źródło:
Journal of Machine Engineering; 2017, 17, 4; 83-90
1895-7595
2391-8071
Pojawia się w:
Journal of Machine Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efficient in vitro plant regeneration from cultured leaf and petiole explants of Isatis constricta Davis
Autorzy:
Özel, Ç.A.
Powiązania:
https://bibliotekanauki.pl/articles/11867960.pdf
Data publikacji:
2018
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
Turkey
Brassicaceae
Isatis constricta
plant regeneration
shoot regeneration
leaf
petiole
in vitro method
plant propagation
micropropagation
Opis:
Isatis concstricta Davis (an endemic plant of Turkey) suffers from low propagation rates under natural conditions and is threatened due to fast unplanned urbanisation. The study compared the effects of variants of BA + NAA on shoot regeneration on leaf and petiole explants excised from one week old in vitro regenerated seedlings. MS medium containing 1 mg L–1 BA + 1 mg L–1 NAA induced maximum proliferation on petiole and leaf explants with 13.33 and 12.75 shoots per explant repectively. However, leaf explant induced shoots were sturdy and healthier compared to petiole explant induced shoots. These shoots were rooted on MS medium containing 0.5 mg L–1 IBA and the plants were acclimatized in peat moss and sand (v/v). They grew to flowering under ex vitro conditions. This system of regeneration is advantageous for conventional propagation and the results will help in establishment of a powerful and meaningful micropropagation system for I. constricta.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 3; 49-55
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rooting shoots of apple varieties and their tetraploids obtained by the in vitro technique
Autorzy:
Podwyszyńska, M.
Cieślińska, M.
Powiązania:
https://bibliotekanauki.pl/articles/11888859.pdf
Data publikacji:
2018
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant breeding
Malus domestica
Free Redstar cultivar
Gala Must cultivar
Pinova cultivar
Red Chief cultivar
plant cultivar
fruit tree
apple tree
shoot rooting
in vitro method
tetraploid
Opis:
For breeding purposes, the number of neo-tetraploids of apple cultivars have been derived by in vitro technique. The first attempts at rooting and acclimatization of tetraploid shoots failed. The aim of the study was to develop an effective method for rooting microcuttings of apple neo-tetraploids. In the first stage of the study, in vitro rooting method was optimized for the shoots of diploid donor cultivars. Shoots were rooted on Murashige and Skoog [1962] (MS) medium with a reduced content of nitrogen, in the presence of auxins alone or in combination (indole-3-butyric acid – IBA, 1-naphthaleneacetic acid – NAA and indole- -3-acetic acid – IAA) with addition of putrescine, arginine or ornithine. The compounds were applied continuously for 25 days (one-step rooting system) or for seven days with subsequent transplanting shoots onto a medium without these compounds (two-step rooting method). Tetraploid microcuttings of the cultivars ‘Free Redstar’, ‘Gala Must’, ‘Pinova’ and ‘Redchief’ were evaluated for rooting on the selected medium considered optimal for their diploid counterparts. The shoots of all diploid apple scion cultivars had low rooting capacity. IBA alone poorly stimulated root formation. Significant improvement of rooting to 60–80% was achieved through the application of auxins, 2.5 µM IBA or 1.3 µM NAA combined with 5 µM IAA and 50 µM putrescine in the two-step rooting system with darkness and increased temperature of 26°C during seven-day induction phase. The replacement of benzyladenine (BA) by meta-Topolin (m-T) in the last multiplication subculture influenced positively shoot acclimatization. Tetraploids had comparable or slightly lower rooting and acclimatization ability compared to their diploid counterparts.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 1; 49-62
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro evaluation of potato genotypes for resistance against bacterial soft rot (Pectobacterium carotovorum) - a new tool for studying disease resistance
Autorzy:
Azadmanesh, Sima
Mozafari, Javad
Hasanzadeh, Nader
Moslemkhani, Cobra
Powiązania:
https://bibliotekanauki.pl/articles/961645.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
in vitro
potato soft rot
resistance
screening method
Opis:
In vitro screening techniques were used to evaluate 46 genotypes of Iranian potato collection for resistance to bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc). One month old in vitro rooted potato plantlets were inoculated by two inoculation techniques under in vitro conditions: 1) sterile toothpicks dipped into bacterial suspension and pressed into the crown of plantlets and 2) the freshly cut crown of plantlets were dipped into bacterial suspension of 108 cfu ∙ ml–1 for 10 min. Typical soft rot disease symptoms, including the percentage of wilted leaves were recorded on inoculated plantlets 3, 6, 9, 12 and 15 days post-inoculation. The potato genotypes which were examined responded differently to Pcc and varying levels of resistance were observed. Potato genotype AG showed the highest level of resistance. Results obtained from in vitro screening were then verified by classical tuber slice assay. The verifications were conducted using five representative cultivars: Milva, Ramus, Picaso, Marfona and Agria which responded similarly to both in vitro and classical evaluation systems. Similar results obtained from these tests indicated that the in vitro screening technique developed in this study could provide a simple and rapid whole plant assay in selecting resistant potato genotypes against bacterial soft rot.
Źródło:
Journal of Plant Protection Research; 2017, 57, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pollen viability and in vitro germination of selected Central European species from genus Rosa analysed with different methods
Autorzy:
Wronska-Pilarek, D.
Tomlik-Wyremblewska, A.
Powiązania:
https://bibliotekanauki.pl/articles/41113.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
pollen viability
in vitro germination
germination
Rosaceae
European species
Rosa
analysis method
different method
Opis:
The main aim of the study was to determine which of the viability and germination capacity of pollen grains estimation methods are optimal and to test if the studied sections and species within the Rosa genus are differentiated in pollen viability andgermination capacity. We analysedandcomparedpollen grains of 14 wild Rosa species using 5 viability tests and13 various liquidandagar media. Viability of pollen grains in the majority of the examinedspecies was greater than their germination capacity. The most viable pollen grains were foundin R. gallica (section Gallicanae), where average of 5 viability tests is 90.69%, andin R. pendulina (section Rosa) – 86.85%. Species from section Caninae have a lower level of viability (from 60.59% in R. rubiginosa to 31.23% in R. inodora). Pollen of species from sections Gallicanae (R. gallica; to 63.4%) and Rosa (R. pendulina; to 47.21%) germinatedmuch better than the pollen of the majority of species from section Caninae (with the exception of R. zalana; to 47.22%). The examinedpollen grains germinatedmost numerously on agar medium with 1.5% agar+15% sucrose+50 ppm boric acidandliquidmed ium with 15% sucrose+ 50 ppm boric acid. Following deep-freeze storage (–25°C) for six months, the pollen grains of nine selected species (with the exception of R. rubiginosa) showed viability higher or similar to that before storage.
Źródło:
Dendrobiology; 2010, 64
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Numerical optimization of the BAT-CELL Bio-Ambient-Tests method for engine exhausts toxicity evaluation
Autorzy:
Kęska, Aleksandra
Janicka, Anna
Zawiślak, Maciej
Powiązania:
https://bibliotekanauki.pl/articles/24202411.pdf
Data publikacji:
2023
Wydawca:
Polskie Towarzystwo Naukowe Silników Spalinowych
Tematy:
internal combustion engine
Euro norms
new method
in vitro tests
silnik o spalaniu wewnętrznym
normy europejskie
nowa metoda
badania in vitro
Opis:
The BAT-CELL Bio-Ambient-Tests method is based on the assessment of the influence of the actual toxicity of various types of gas mixtures on living cells, taking into account the additive synergism. Work has been carried out on the application of the BAT-CELL method for testing engine exhaust gases. The application of computational fluid mechanics using Ansys Fluent made it possible to analyse the flow of engine exhaust gases through the aspiration system used, including analysis of shear stress values and their uniformity distribution on the bottom wall of the sampler containing cell culture on the bottom wall of the sampler. The appropriate flow rate of exhaust gases through the aspiration system and the shape of aspiration tubing for the sampler were selected in order to enable uniform contact of gas particles with the cell surface and not to damage them mechanically. The simulation results were verified in real-life tests and confirmed the theoretical assumptions.
Źródło:
Combustion Engines; 2023, 62, 1; 19--25
2300-9896
2658-1442
Pojawia się w:
Combustion Engines
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Digestion and absorption of phenolic compounds assessed by in vitro simulation methods. A review
Autorzy:
Tarko, T.
Duda-Chodak, A.
Zajac, N.
Powiązania:
https://bibliotekanauki.pl/articles/873750.pdf
Data publikacji:
2013
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
plant metabolite
fruit
vegetable
antioxidant property
phenolic compound
in vitro digestion
simulation method
Caco-2 cell
gastrointestinal tract
gut
microflora
biological property
Opis:
Phenolic compounds are a group of key plant metabolites found abundantly in fruit and vegetables. Because of their antioxidant properties, they play a significant role in preventing various degenerative illnesses, tumours or cardiovascular disease. In nature, they are present in foods mainly as esters, glycosides and polymers which need to undergo enzymatic hydrolysis in the digestive tract or by the gut microflora before becoming absorbed. The biological properties of these phenolic compounds undergoing this degradation, are thus governed by their absorption as well as metabolism. Many methods are used to assess the rates and the degrees to which these substances are digested and absorbed, both in vivo and in vitro ones, where the former are the most reliable, although they suffer from various limitations. For this reason, many in vitro models have now arisen to simulate the function of human digestion in the attempt to faithfully re-create real-life conditions. Mechanisms of polyphenols absorption have been principally studied by intestinal epithelial cell models, in particular, those using the Caco-2 cell line.
Związki fenolowe są grupą roślinnych metabolitów występujących obficie w owocach i warzywach. Dzięki swoim właściwościom antyoksydacyjnym odgrywają istotną rolę w prewencji chorób degeneracyjnych, np. nowotworów lub chorób układu krwionośnego. W naturze większość związków polifenolowych jest obecna w pożywieniu w postaci estrów, glikozydów i polimerów, które aby ulec absorpcji muszą zostać zhydrolizowane przez enzymy trawienne bądź mikroflorę jelitową. Absorpcja oraz metabolizm związków polifenolowych zachodzące w przewodzie pokarmowym człowieka wpływa na ich właściwości biologiczne. Istnieje wiele metod in vivo oraz in vitro oceniających stopień i szybkość trawienia oraz przyswajanie związków polifenolowych. Metodami zapewniającymi uzyskanie najbardziej pewnych wyników są metody in vivo, jednak posiadają one wiele ograniczeń. Dlatego też powstało wiele modeli in vitro symulujących pracę układu pokarmowego człowieka i odtwarzających wiernie warunki w nim panujące. Do badań nad mechanizmami absorpcji związków polifenolowych przez nabłonek jelit używane są modele kultur nabłonkowych in vitro, w szczególności z wykorzystaniem linii komórkowych Caco-2.
Źródło:
Roczniki Państwowego Zakładu Higieny; 2013, 64, 2
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on solid, liquid and double-phase culture systems
Autorzy:
Kaviani, B.
Bahari, B.
Powiązania:
https://bibliotekanauki.pl/articles/12678778.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant cultivation
Gentianaceae
Eustoma
Lisianthus
Eustoma grandiflorum
ornamental plant
flowering plant
rooting
microshoot
micropropagation
in vitro proliferation
ex vitro method
soild phase
liquid phase
double-phase culture
plant growth regulator
auxin
cytokinin
Opis:
A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and 5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with 0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 47-56
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-8 z 8

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