Numerical optimization of the BAT-CELL Bio-Ambient-Tests method for engine exhausts toxicity evaluation

The BAT-CELL Bio-Ambient-Tests method is based on the assessment of the influence of the actual toxicity of various types of gas mixtures on living cells, taking into account the additive synergism. Work has been carried out on the application of the BAT-CELL method for testing engine exhaust gases. The application of computational fluid mechanics using Ansys Fluent made it possible to analyse the flow of engine exhaust gases through the aspiration system used, including analysis of shear stress values and their uniformity distribution on the bottom wall of the sampler containing cell culture on the bottom wall of the sampler. The appropriate flow rate of exhaust gases through the aspiration system and the shape of aspiration tubing for the sampler were selected in order to enable uniform contact of gas particles with the cell surface and not to damage them mechanically. The simulation results were verified in real-life tests and confirmed the theoretical assumptions.