- Tytuł:
- Disruption of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition
- Autorzy:
-
Górka-Nieć, Wioletta
Pniewski, Michał
Kania, Anna
Perlińska-Lenart, Urszula
Palamarczyk, Grażyna
Kruszewska, Joanna - Powiązania:
- https://bibliotekanauki.pl/articles/1040737.pdf
- Data publikacji:
- 2008
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
Trichoderma reesei
pmt1 gene disruption
protein glycosylation
cell wall composition - Opis:
- In fungi transfer of the first mannosyl residue to proteins during their O-glycosylation is catalyzed by protein O-mannosyltransferases encoded by pmt genes. Disruption of the pmt1 gene in Trichoderma caused a significant decrease in the total activity of protein O-mannosyltransferases. Moreover, disruption of the pmt1 gene also led to osmotic sensitivity of the strain, indicating an essential role of the PMTI protein activity for cell wall synthesis. At the same time, the strain was defective in septa formation, producing only half the number of septa per unit length of hypha compared with the wild type. Disruption of the pmt1 gene decreased protein secretion but had no effect on glycosylation of secreted proteins, which suggests that PMTI protein O-mannosyltranferase does not take part in glycosylation of these proteins.
- Źródło:
-
Acta Biochimica Polonica; 2008, 55, 2; 251-259
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł