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Wyszukujesz frazę "Yang, Z." wg kryterium: Autor


Wyświetlanie 1-15 z 15
Tytuł:
Projective nonnegative matrix factorization based on α-divergence
Autorzy:
Yang, Z.
Oja, E.
Powiązania:
https://bibliotekanauki.pl/articles/91672.pdf
Data publikacji:
2011
Wydawca:
Społeczna Akademia Nauk w Łodzi. Polskie Towarzystwo Sieci Neuronowych
Tematy:
Nonnegative Matrix Factorization
NMF
α-divergence
PNMF
α-NMF
α-PNMF
Opis:
The well-known Nonnegative Matrix Factorization (NMF) method can be provided with more flexibility by generalizing the non-normalized Kullback-Leibler divergence to α- divergences. However, the resulting α-NMF method can only achieve mediocre sparsity for the factorizing matrices. We have earlier proposed a variant of NMF, called Projective NMF (PNMF) that has been shown to have superior sparsity over standard NMF. Here we propose to incorporate both merits of α-NMF and PNMF. Our α-PNMF method can produce a much sparser factorizing matrix, which is desired in many scenarios. Theoretically, we provide a rigorous convergence proof that the iterative updates of α-PNMF monotonically decrease the α-divergence between the input matrix and its approximate. Empirically, the advantages of α-PNMF are verified in two application scenarios: (1) it is able to learn highly sparse and localized part-based representations of facial images; (2) it outperforms α-NMF and PNMF for clustering in terms of higher purity and smaller entropy.
Źródło:
Journal of Artificial Intelligence and Soft Computing Research; 2011, 1, 1; 7-16
2083-2567
2449-6499
Pojawia się w:
Journal of Artificial Intelligence and Soft Computing Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reliability modeling of fault tolerant control systems
Autorzy:
Li, H.
Zhao, Q.
Yang, Z.
Powiązania:
https://bibliotekanauki.pl/articles/929822.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
sterowanie tolerujące uszkodzenia
ocena niezawodności
procesy semimarkowskie
fault tolerant control
reliability evaluation
semi-Markov processes
Opis:
This paper proposes a novel approach to reliability evaluation for active Fault Tolerant Control Systems (FTCSs). By introducing a reliability index based on the control performance and hard deadline, a semi-Markov process model is proposed to describe system operation for reliability evaluation. The degraded performance of FTCSs in the presence of imperfect Fault Detection and Isolation (FDI) is reflected by semi-Markov states. The semi-Markov kernel, the key parameter of the process, is determined by four probabilistic parameters based on the Markovian model of FTCSs. Computed from the transition probabilities of the semi-Markov process, the reliability index incorporates control objectives, hard deadline, and the effects of imperfect FDI, a suitable quantitative measure of the overall performance.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2007, 17, 4; 491-504
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Integrated decision on spare parts ordering and equipment maintenance under condition based maintenance strategy
Zintegrowany system decyzyjny dotyczący zamawiania części zamiennych i utrzymania ruchu urządzeń w ramach strategii utrzymania zależnej od bieżącego stanu technicznego
Autorzy:
Wang, Y.
Zhao, J.
Cheng, Z.
Yang, Z.
Powiązania:
https://bibliotekanauki.pl/articles/301823.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
spare parts
inventory control
ordering
condition based maintenance
Monte Carlo simulation
części zamienne
kontrola zapasów
zamówienia
utrzymanie zależne od bieżącego stanu technicznego
symulacja Monte Carlo
Opis:
Aiming to optimize the equipment maintenance and the spare parts ordering management jointly, a comprehensive decision model under condition based maintenance (CBM) policy is presented for a single equipment system with continuous and random deterioration. In this model, the equipment deterioration is a continuous Gamma process under a continuous condition monitoring, and the spare parts inventory is controlled by spare parts support probability. Firstly, a spare part support probability model was developed to determine the optimal spare parts stock level S, which is set to meet the requirement of a predetermined stockout probability. Secondly, the equipment replacement and spare parts ordering decision is made to optimize the equipment replacement and spare parts ordering jointly, which is based on the equipment deterioration leveland total operating cost of the system. Thirdly, an integrated decision simulation model is presented for evaluating cost rate, availability and stockout probability. Finally, a numerical example is given to illustrate the performance of this model. The results show that the optimal preventive maintenance threshold obtained from the proposed decision model can satisfy the spare parts support requirements under (S-1, S) inventory control strategy.
Dążąc do jednoczesnej optymalizacji utrzymania ruchu urządzeń i zarządzania zamówieniami części zamiennych, zaproponowano kompleksowy model decyzyjny w ramach strategii utrzymania zależnej od bieżącego stanu technicznego (CBM) przeznaczony dla systemów z pojedynczym urządzeniem i ciągłym oraz losowym zużyciem. W niniejszym modelu, zużycie urządzenia jest ciągłym procesem Gamma z ciągłym monitorowaniem stanu, podczas gdy zapasy części zamiennych są kontrolowane poprzez prawdopodobieństwo wsparcia w zakresie części zamiennych. Po pierwsze, opracowano model prawdopodobieństwa wsparcia w zakresie części zamiennych w celu określenia optymalnego poziomu zapasów części zamiennych S, ustalonej aby spełnić wymogi określonego prawdopodobieństwa braku dostępności. Po drugie, przeprowadzono proces decyzyjny dotyczący wymiany urządzenia i zamawiania części zamiennych w celu jednoczesnej optymalizacji wymiany urządzenia i zamawiania części zamiennych, w oparciu o poziom zużycia urządzenia i całkowity koszt działania systemu. Po trzecie, zaprezentowano zintegrowany symulacyjny model decyzyjny dla oceny poziomu kosztów, dostępności i prawdopodobieństwa jej braku. Zasady niniejszego modelu zilustrowano przykładem numerycznym. Wyniki pokazują, że optymalny próg konserwacji zapobiegawczej uzyskany za pomocą proponowanego modelu decyzyjnego może spełnić wymagania dotyczące części zamiennych w ramach (S-1, S) strategii kontroli zapasów.
Źródło:
Eksploatacja i Niezawodność; 2015, 17, 4; 591-599
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Spin-Spin and Spin-Other-Orbit Interactions of Spin-Hamiltonian Parameters for $3d^{2(8)}$ Ions in $Al_2O_3$ Crystals
Autorzy:
Wei, Q.
Guo, L.
Yang, Z.
Wei, B.
Powiązania:
https://bibliotekanauki.pl/articles/1505102.pdf
Data publikacji:
2011-06
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
76.30.Fc
71.70.Ch
75.10.Dg
71.55.Ht
Opis:
By considering weaker magnetic interactions (including spin-spin and spin-other-orbit interactions) in Hamiltonian, the spin-Hamiltonian parameters, including the zero-field splitting parameter D and g factors $(g_∥, g_⊥)$ for $3d^{2(8)}$ ions in $Al_2O_3$ crystals have been investigated. The results show that the contributions to D, $g_∥$ and $g_⊥$ from the spin-orbit interaction are dominant. The contributions from weaker magnetic interactions to D decrease (increase) with increasing charge of impurity ions for $3d^2 (3d^8)$ ions.
Źródło:
Acta Physica Polonica A; 2011, 119, 6; 857-859
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An integrated treatment technology for ground fissures of shallow coal seam mining in the mountainous area of southwestern China: a typical case study
Zintegrowana technologia zapobiegania pęknięciom gruntu w procesie wydobywania płytkich pokładów węgla w górzystym obszarze południowo-zachodnich Chin: typowe studium przypadku
Autorzy:
Zhu, H.
He, F.
Zhang, S.
Yang, Z.
Powiązania:
https://bibliotekanauki.pl/articles/215969.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Instytut Gospodarki Surowcami Mineralnymi i Energią PAN
Tematy:
wydobywanie węgla
płytki pokład węgla
pęknięcia gruntu
zapadlisko górnicze
zintegrowana technologia zapobiegania
ochrona środowiska
shallow coal seam mining
ground fissures
surface sinkhole
integrated treatment technology
environmental protection
Opis:
This article is the result of treatments on ground fissures for environmental protection and scientific shallow coal seam mining. In the southwestern mining area of China, the traditional longwall mining method has caused a large area of surface sinkhole, ground fissures, vegetation deterioration and disorderly coal gangue. To solve these problems, an integrated treatment technology that includes ground fissure treatment technology and underground backfilled technology was proposed as a solution. The technical principle and technical process were explained in detail; the ground fissure treatment technology involves a “three-step treatment method”, and the underground backfilled technology adopted a strip mining method with backfilling technology. The compression mechanical behavior of backfilled material, including coal gangue, fly ash and ordinary Portland cement, was studied; the mixed ratio of 1:0.3:0.18 was selected. In addition, the vertical stress, vertical displacement and plastic zone of the coal pillar were determined by FLAC3D numerical simulation, and a rational mining scheme of “11 m mining width, 2 m coal pillar width” was determined to be appropriate because of the lower vertical stress, smaller vertical displacement and better supporting capacity of the coal pillar. The monitoring results of ground sinkhole indicated that the maximal ground sinkhole deformation was 17.3 cm, and the deformation showed few changes after this technology was implemented. The treatment capacity of coal gangue and fly ash reached 821.150 t per year, and the vegetation survival rate of the ground fissure treatment area reached 85%. This integrated treatment technology could effectively control ground fissures and surface sinkhole as well as protect the environment.
Artykuł jest opisem zapobiegania pęknięciom gruntu w celu ochrony środowiska, jak również naukowego podejścia do wydobywania płytko zalegających pokładów węgla. W południowo-zachodniej części górniczej Chin tradycyjna, ścianowa metoda wydobywania węgla, powodowała duży obszar powierzchniowego zapadliska, pęknięcia gruntu, pogorszenie stanu wegetacji roślin. W celu rozwiązania tych problemów zaproponowano zintegrowaną technologię zapobiegania, która obejmuje technologię zapobiegania pęknięciom gruntu i podziemną technologię podsadzki. Zasada i proces techniczny zostały szczegółowo opisane; technologia zapobiegania pęknięciom gruntu obejmuje „trójstopniową metodę zabiegu”, a technologia podsadzki zaadoptowała metodę wydobywania węgla pasami. Badano ściśliwość podsadzki, w tym odpadów węglowych, popiołu lotnego i zwykłego cementu portlandzkiego; wybrano mieszaninę o stosunku 1: 0,3: 0,18. Ponadto pionowe naprężenie, pionowe przemieszczenie i strefa plastyczności filaru węglowego zostały określone za pomocą symulacji numerycznej FLAC 3D. Racjonalny schemat wydobycia „szerokość wydobycia 11 m, szerokość filaru węglowego 2 m” był odpowiedni ze względu na niższą wartość naprężenia pionowego, mniejsze pionowe przemieszczenie i lepszą nośność filaru węglowego. Wyniki monitoringu pęknięć gruntu wskazują, że maksymalna deformacja gruntu wynosiła 17,3 cm i wykazała kilka zmian po wdrożeniu tej technologii. Ilość zagospodarowanych odpadów węglowych i popiołu lotnego wynosiła 821 150 Mg na rok, a wskaźnik przeżycia roślinności w obszarze zapobiegania pęknięciom gruntu osiągnął poziom 85%. Ta zintegrowana technologia zapobiegania może skutecznie kontrolować pęknięcia gruntu i deformację powierzchni, a także chronić środowisko.
Źródło:
Gospodarka Surowcami Mineralnymi; 2018, 34, 1; 119-138
0860-0953
Pojawia się w:
Gospodarka Surowcami Mineralnymi
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of heating uniformity in radio frequency heating systems using carrot and radish
Autorzy:
Kong, L.
Min, Z.
Wang, Y.
Adhikari, B.
Yang, Z.
Powiązania:
https://bibliotekanauki.pl/articles/25929.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Opis:
Lack of heating uniformity is a major problem impeding the broader adaptation of radio frequency heaters in industrial applications. The overall aim of this study was to evaluate the uniformity of heating or temperature distribution within food samples (three different carrot and one radish rectangles) placed vertically and horizontally within a radio frequency heating cavity. The intensity of the electric field in radio frequency was found to be symmetrical. The temperatures at the vertically top positions were lower than the vertically bottom positions at the equidistance from the vertical center with the highest was at the vertically central position. The rate of temperature rise at all the positions were higher in taller (higher mass) than the shorter (lower mass) rectangles of carrots. The temperatures at the corners and edges were lower than at the cross sectionally central positions at all the heights tested in both carrots and radishes. The rate of temperature rise at all the vertical positions was higher in radish rectangles than in the carrot rectangles of the same dimensions. The similarity of temperature distribution in carrot and radish rectangles suggested that the heating patterns and uniformity in carrots and radishes in RF heating were almost the same.
Źródło:
International Agrophysics; 2016, 30, 4
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
HPM Generation by Tri-Anode Coaxial Vircator
Autorzy:
Shao, H.
Liu, G.
Zhang, Y.
Yang, Z.
Sun, J.
Powiązania:
https://bibliotekanauki.pl/articles/1807906.pdf
Data publikacji:
2009-06
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
75.47.Gk
Opis:
A novel type of coaxial vircator with 3 anodes is proposed. The advantages on high power microwave generation is tested by particle-in-cell simulation and theoretical analysis model is set up as well. The tri-anode coaxial vircator behaves a very stable frequency in the pulse duration. In the tri-anode coaxial vircator, the injecting electron beam is well premodulated in the first anode cavity and forms an inerratic bunch-dispersion mechanism in the second anode cavity; the energy-loss electrons penetrate through the third anode and absorbed by the dumping cavity locates in the centre electrode, which helps keeping the bunch-dispersion cycle undisturbed. A 3.8 GHz tri-anode coaxial vircator is theoretically designed and optimized by particle-in-cell simulation, which gives beam-wave conversion efficiency of over 20% with a stable predominant frequency, in accordance with the particle-in-cell simulation and analytic model.
Źródło:
Acta Physica Polonica A; 2009, 115, 6; 1047-1049
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Design and analysis of interior composite-rotor bearingless permanent magnet synchronous motors with two layer permanent magnets
Autorzy:
Sun, X.
Su, B.
Chen, L.
Yang, Z.
Li, K.
Powiązania:
https://bibliotekanauki.pl/articles/200490.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bearingless motor
permanent magnet motor
finite element analysis
cogging torque
static electromagnetic characteristics
silnik z magnesem trwałym
analiza elementów skończonych
moment skręcający
właściwości elektromagnetyczne
silnik bezłożyskowy
Opis:
In this paper, a new type of interior composite-rotor bearingless permanent magnet synchronous motors (BPMSMs) with two layer permanent magnets (PMs) is proposed. In order to reduce the torque ripple of this kind of motors, the sizes of PMs are optimized. Moreover, the magnetic field analysis of the interior composite-rotor BPMSM with two layer PMs is carried out by the finite element method (FEM). The corresponding static electronic magnetic characteristics at no load, including magnetic field, PM flux linkage and inductance, are studied in detail. In addition, electromagnetic torque characteristics and suspension force characteristics are also investigated thoroughly. The results of the analysis and simulation lay a significant foundation for further research on the interior composite-rotor BPMSMs with two layer PMs.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2017, 65, 6; 833-843
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative study of fault-tolerant performance of a segmented rotor SRM and a conventional SRM
Autorzy:
Sun, X.
Xue, Z.
Han, S.
Chen, L.
Xu, X.
Yang, Z.
Powiązania:
https://bibliotekanauki.pl/articles/200893.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
fault tolerance
segmented rotor SRM
phase failure
finite element method
dynamic performance
rotor segmentowy SRM
metoda elementów skończonych
wydajność dynamiczna
tolerancja błędu
awaria jednej fazy
Opis:
Due to the separation of magnetic field, electrical isolation and thermal isolation, motor drives possess a high fault-tolerance characteristic. In this paper, comparative study of mutual inductance between the proposed segmented rotor switched reluctance motor (SSRM) and the conventional switched reluctance motor (SRM) is carried out first, illustrating that the proposed SSRM has less mutual inductance between phases than the conventional SRM. In addition, if winding faults or power converter faults lead to phase failure, a comparative analysis on fault-tolerant performance under phase failure condition between the proposed SSRM and the conventional SRM is simulated in detail using the finite element method (FEM). Simulation results reveal that dynamic performance of the proposed SSRM, including output torque and phase current, is better than that of the conventional SRM. That is, the capacity of operating with the fault under phase failure condition in the proposed SSRM is superior to that in the conventional SRM.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2017, 65, 3; 375-381
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fatigue life prediction of umbilicals considering non-linear stress
Autorzy:
Lu, Q.
Yin, Y.
Yang, Z.
Chen, J.
Yan, J.
Yue, Q.
Powiązania:
https://bibliotekanauki.pl/articles/259519.pdf
Data publikacji:
2017
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
steel tube umbilical
fatigue life
contact and friction
non-linear local stress
Opis:
The fatigue problem induced by wave loads and floater movements of dynamic umbilicals in deepwater is studied. The prediction method of the fatigue life is investigated by considering no-linear local stress due to contact and friction between components of the umbilical. A case study of a dynamic umbilical for 1500 meters depth in South China Sea was presented. The results showed that the more accurate result of the fatigue life was calculated by considering non-linear local stress. The fatigue life by considering local stress with the no-slip assumption was conservative. An obviously longer fatigue life was obtained by considering local stress with the full-slip assumption.
Źródło:
Polish Maritime Research; 2017, S 2; 154-163
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Digital control system design for bearingless permanent magnet synchronous motors
Autorzy:
Sun, X.
Shi, Z.
Yang, Z.
Wang, S.
Su, B.
Chen, L.
Li, K.
Powiązania:
https://bibliotekanauki.pl/articles/202311.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
BPMSMs
cyfrowy system kontroli
podwójnie zamknięty system regulacji prędkości
projektowanie oprogramowania
projekt sprzętu
digital control system
double-closed speed regulating system
software design
hardware design
Opis:
This study investigates a digital control system which is used in bearingless permanent magnet synchronous motors (BPMSMs). Compared with traditional permanent magnet synchronous motors, a BPMSM is characterized by higher speed and no mechanic friction. Therefore, the application value of the latter to the special area is higher than that of the former. An analysis from previous work on the BPMSM had proved its feasibility, and some performances such as suspension force, inductance and so on were also investigated. Based on this analysis, this study focuses on solving control problems in practical applications by designing a control system. The control system design includes overall schematic, hardware and software designs. Main software systems, including the force/current transform module and closed loop control module for radial displacement, are analyzed. Interface circuit for radial displacement, current feedback circuit and dead zone protection circuit are designed for the hardware system. Finally, several performance experiments have been conducted to verify the effectiveness of the designed digital control system. Experiment results indicate that the rotor has unique characteristics, such as stable suspension performance, good start-of-suspension performance, and rapid anti-disturbance features.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2018, 66, 5; 687-698
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of knock suppression characteristics in a boosted methane : gasoline blended fuelled SI engine
Autorzy:
Yang, Z.
Miganakallu, N.
Rao, S.
Harsulkar, J.
Naber, J.
Lonari, Y.
Szwaja, S.
Powiązania:
https://bibliotekanauki.pl/articles/244629.pdf
Data publikacji:
2018
Wydawca:
Instytut Techniczny Wojsk Lotniczych
Tematy:
knock
methane
gasoline
E10
blend fuel
knock onset prediction
simulation
Opis:
Natural gas has a higher knock suppression effect than gasoline which makes it possible to operate at higher compression ratio and higher loads resulting in increased thermal efficiency in a spark ignition engine However, using port fuel injected natural gas instead of gasoline reduces the volumetric efficiency from the standpoints of the charge displacement of the gaseous fuel and the charge cooling that occurs from liquid fuels. This article investigates the combustion and engine performance characteristics by utilizing experimental and simulation methods varying the natural gas-gasoline blending ratio at constant engine speed, load, and knock level. The experimental tests were conducted on a single cylinder prototype spark ignited engine equipped with two fuel systems: (i) a Direct Injection system for gasoline and (ii) a Port Fuel Injection (PFI) system for compressed natural gas. For the fuels, gasoline with 10% ethanol by volume (commercially known as E10) with a research octane number of 91.7 is used for gasoline via the DI system, while methane is injected through PFI system. The knock suppression tests were conducted at 1500 rpm, 12 bar net indicated mean effective pressure wherein the engine was boosted using compressed air. At 60% of blending methane with E10 gasoline, the results show high knock suppression. The net indicated specific fuel consumption is 7% lower, but the volumetric efficiency is 7% lower compared to E10 gasoline only condition. A knock prediction model was calibrated in the 1-D simulation software GT-Power by Gamma Technologies. The calibration was conducted by correlating the simulated engine knock onset with the experimental results. The simulation results show its capability to predict knock onset at various fuel blending ratios.
Źródło:
Journal of KONES; 2018, 25, 3; 517-525
1231-4005
2354-0133
Pojawia się w:
Journal of KONES
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein
Autorzy:
Wu, X.
Xiao, L.
Peng, B.
Wang, Y.
Yang, Z.
Yao, X.
Hu, L.
Lin, X.
Powiązania:
https://bibliotekanauki.pl/articles/30963.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Construction of a recombinant baculovirus expressing swine hepatitis E Virus ORF2 and preliminary research on its immune effect
Autorzy:
Yang, Z.
Wang, K.
Hu, Y.
Yuan, P.
Yang, Y.
Xie, L.Y.
Huang, S.L.
Liu, J.
Ran, L.
Song, Z.H.
Powiązania:
https://bibliotekanauki.pl/articles/2087759.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
swine HEV
ORF2
baculovirus
immunogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 47-54
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Microstructural Evolution in HMX Based Plastic-bonded Explosive During Heating and Cooling Process: an in situ Small-angle Scattering Study
Autorzy:
Yan, G.
Tian, Q.
Liu, J.
Fan, Z.
Sun, G.
Zhang, C.
Wang, Y.
Chen, B.
Gong, J.
Zhou, X.
Yang, Z.
Nie, F.
Li, J.
Li, X.
Powiązania:
https://bibliotekanauki.pl/articles/358618.pdf
Data publikacji:
2016
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
SANS
SAXS
HMX-PBX
thermal damages
phase transition
Opis:
The thermal damage in octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) based plastic-bonded explosive (PBX) was investigated using in situ small-angle neutron and X-ray scattering techniques. The microstructural evolution was quantitatively characterized by the model fitting parameters of total interfacial surface area (Sv) and void volume distribution. The Sv of HMX-PBX decreased markedly above 100 °C, indicating the movement of binder into the voids. After subsequent cooling to room temperature, the scattering intensity increased significantly with increasing storage time, and a new population of voids with average diameter of 20 nm was observed, accompanied by the gradual phase transition of HMX from δ- to β-phase. The experimental results implied that serious damage within the HMX-PBX was developed during storage after heating.
Źródło:
Central European Journal of Energetic Materials; 2016, 13, 4; 916-926
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
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