Temat: Pseudomonas - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Diversity of Pseudomonas species associated with soft rot of plants in Poland
Autorzy:: Zolobowska, L
Pospieszny, H.
Powiązania:: https://bibliotekanauki.pl/articles/65211.pdf
Data publikacji:: 2001
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Pseudomonas fluorescens
Polska
Pseudomonas
polymerase chain reaction
occurrence
Pseudomonas putida
heterogeneity
identification
plant
Opis:: A total of 94 pectolytic and 60 nonpectolytic Pseudomonas isolates were obtained from 250 samples of rotted vegetable specimens representing various economically important vegetables. The isolates were identified on the basis of standard biochemical tests. Pseudomonas fluorescens biovar V and II and Pseudomonas putida were the most abundant species among pectolytic isolates and Pseudomonas fluorescens biovar I among nonpectolytic ones. Only 3 Pseudomonas viridiflava isolates were identified and all of them were obtained from potato. Isolates of pectolytic phenotype were scattered among nonpectolytic ones irrespective of their taxonomical status. Isolates identified biochemically, as Pseudomonas marginalis were also present in nonpectolytic group. PCR method is unsuitable for identification and differentiation of bacteria belonging to pectolytic fluorescens Pseudomonas group due to great diversity of species. However, the results of PCR amplification of the genes encoding pectate lyase suggest that genes responsible for production of this enzyme may also be present in isolates of nonpectolytic phenotype.
Z 250 prób, z różnych gatunków roślin warzywnych z objawami mokrej zgnilizny wyodrębniono 94 izolaty pektynolitycznych i 60 izolatów nie pektynolitycznych bakterii z rodzaju Pseudomonas. Identyfikację i różnicowanie izolatów przeprowadzono przy zastosowaniu standardowych testów fizjologiczno-biochemicznych. Spośród izolatów z grupy pektolitycznych Pseudomonas najliczniej występował gatunek P. fluorescens, który był reprezentowany przez 4 biowary (oprócz czwartego), przy czym dominowały biowary II i V. Mniej licznie występował gatunek P. putida, a P. viridiflava był reprezentowany jedynie przez 3 izolaty, pochodzące z ziemniaka. Podobna była struktura populacji izolatów z grupy niepektolitycznych Pseudomonas. Metoda PCR okazała się być mało przydatna do wykrywania i różnicowania izolatów z grupy pektolitycznych Pseudomonas ze względu na duże ich zróżnicowanie. Amplifikacja DNA metodą PCR wykazała, że geny kodujące enzym liazy pektynowej występują także w izolatach niepektolitycznych rodzaju Pseudomonas.
Źródło:: Journal of Plant Protection Research; 2001, 41, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: A comparison between Pseudomonas aureofaciens (chlororaphis) and P. fluorescens in biological control of cotton seedling damping-off disease
Autorzy:: Samavat, S.
Heydari, A.
Zamanizadeh, H.R.
Rezaee, S.
Aliabadi, A.A.
Powiązania:: https://bibliotekanauki.pl/articles/66475.pdf
Data publikacji:: 2014
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Pseudomonas aureofaciens
Pseudomonas fluorescens
biological control
cotton
seedling
damping off disease
plant disease
Opis:: Due to the importance of the biological control of plant diseases, testing and introducing new biocontrol-active microorganisms is a major concern among plant pathologists. The causal agent of cotton seedling damping-off disease is Rhizoctonia solani. In this regard, we tried to investigate the antagonistic activities of Pseudomonas aureofaciens (chlororaphis) 30–84 (phenazine producing wild type and non-phenazine producing mutant) strains on R. solani, in comparison with some isolates of P. fluorescent under both in vitro (laboratory) and in vivo (greenhouse) conditions. In the laboratory experiment, the inhibitory effects of all the bacteria, on the growth of R. solani, were evaluated using the dual culture procedure. Results showed that five isolates of P. fluorescent along with both strains of P. aureofaciens significantly inhibited the growth of R. solani. Effective bacterial antagonists were then evaluated in a greenhouse experiment where cotton seeds were coated with their suspensions and were sown in pasteurised field-soil. The soil had been pre-inoculated with a virulent isolate of R. solani. The efficacy of the bacterial antagonists was evaluated by counting the number of surviving seedlings in different treatments, at 15 and 60 days after sowing, for determining pre- and post-emergence damping-off incidence. According to the results of the greenhouse experiment, at both intervals, two isolates of P. fluorescens along with both strains of P. aureofaciens caused significant increases in the number of healthy seedlings, in comparison with the untreated control, and a commonly used fungicide (carboxin-thiram). The efficacy of phenazine producing a wild type strain of P. aureofaciens was higher than its non-phenazine producing mutant, indicating that phenazine plays an important role in the antagonistic activity of P. aureofaciens. Effective bacterial antagonists were then studied for their antagonistic mechanisms. The results showed that all four bacteria employed different mechanisms. The bacteria produced siderophore, and volatile metabolites and non-volatile metabolites, in their antagonistic activities. The results of this study suggest that P. auerofaciens may be a new biocontrol agent for controlling cotton seedling mortality disease.
Źródło:: Journal of Plant Protection Research; 2014, 54, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Monitoring the occurrence of bacteria in stored cabbage heads
Autorzy:: Eichmeier, Aleš
Peňázová, Eliška
Pečenka, Jakub
Čechová, Jana
Pokluda, Robert
Tekielska, Dorota
Baránek, Miroslav
Powiązania:: https://bibliotekanauki.pl/articles/961651.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: bacteria
cabbage
identifi cation
isolation
mpa medium
pseudomonas
sequencing
16s rrna
Bacillus
Opis:: Twenty-six cabbage heads stored under typical conditions in a storage hall in Moravia, Czech Republic, were tested for the presence of bacteria by the method of isolation from three different parts of the cabbage heads. Isolations were carried out from stalks, inner and superficial leaves. Two samplings were done; in November 2015 and February 2016. Bacterial cultures were sequenced in the part of 16S rRNA region; bacteria were identified according to the sequences obtained. The most prevalent bacteria were of the genus Pseudomonas. Genera: Klebsiella, Erwinia, Pantoea, Bacillus were also identified. The results provided an interesting insight into the bacterial spectrum in stored cabbage heads and their dynamics during storage. Th e nucleotide sequences which were found were saved in GenBank/NCBI under accession numbers KX160104-KX160145.
Źródło:: Journal of Plant Protection Research; 2017, 57, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Production, characterization and iron binding affinity of hydroxamate siderophores from rhizosphere associated fluorescent Pseudomonas
Autorzy:: Deori, M.
Jayamohan, N.S.
Kumudini, B.S.
Powiązania:: https://bibliotekanauki.pl/articles/65031.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: iron-binding compound
hydroxamate siderophore
siderophore
physical parameter
rhizosphere
Pseudomonas
succinate medium
mutation
Opis:: Fluorescent Pseudomonas (FP) is a major group of plant growth promoting rhizobacteria and a well-known synthesizer of siderophores, which imparts a selective advantage on rhizosphere competence and their biocontrol traits. The present study was aimed at examining the factors affecting the production of siderophores and their potential biocontrol traits. Sixteen FP isolates were shortlisted based on their siderophore-producing ability in chrome azural S medium. The isolates were checked for variations in siderophore production under varying incubation times, temperatures, pH, iron (Fe3+) concentrations and mutagens. In addition, the iron binding affinity of siderophores, mycelial inhibition assay and plant growth promotion traits were assessed. Results showed that the siderophore production was highly influenced by the time of incubation, changes in pH, temperature and iron concentration. Chemical characterization showed that the produced siderophores were hydroxamates. Maximum siderophore production was observed at pH 7 whereas UV and EtBr exposure invariably suppressed siderophore production drastically in all isolates. All FPs from maize rhizosphere showed excellent siderophore production which could be due to the competence in strategy-II of the plant rhizosphere and significant growth inhibition on Fusarium oxysporum. Our results suggest the inclination of siderophores to iron, in terms of various criteria affecting production and the possible role of environmental mutations that affect the natural iron harvesting mechanism.
Źródło:: Journal of Plant Protection Research; 2018, 58, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Identification and characterization of Pseudomonas syringae pv. syringae strains from various plants and geographical regions
Autorzy:: Khezri, M.
Mohammadi, M.
Powiązania:: https://bibliotekanauki.pl/articles/65669.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: identification
Pseudomonas syringae pv.syringae
plant
phenotype
polymerase chain reaction
protein profile
plasmid
syringomycin
geographic region
Opis:: Pseudomonas syringae pv. syringae (Pss) constitutes a diverse group of bacterial strains that cause canker of stone fruits, blight of cereals and red streak of sugarcane. The purpose of this study was to determine how diverse Iranian strains of Pss are when they come from different hosts. We compared a total of 32 Pss strains isolated from stone fruits, barley, wheat and sugarcane from different geographical regions of Iran based on their phenotypic and molecular properties. Strains showed some variation regarding carbon and nitrogen utilization. Pss strains were similar in their protein banding patterns. Additional bands were found in sugarcane strains. Most strains showed one indigenous plasmid DNA and a few had two and some none. The genes of syrB and syrD encoding syringomycin synthesis and secretion, respectively, were amplified using specific primers in polymerase chain reaction. Syringomycin, producing strains amplified two DNA fragments of 752 and 446 bp representing syrB and syrD genes, respectively. Primer specificity was shown for Pss using various genera. Based on the results of this study, it is suggested that Pss strains from different hosts and geographical regions show diversity in phenotypic and molecular characters. It is thought that phenotypic variation is due to adaptation to specific hosts and niches for survival and pathogenicity.
Źródło:: Journal of Plant Protection Research; 2018, 58, 4
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Antagonistic bacteria and fungi limiting potato infection by soil-borne pathogenic fungi
Autorzy:: Pieta, D.
Patkowska, E.
Powiązania:: https://bibliotekanauki.pl/articles/66189.pdf
Data publikacji:: 2003
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Trichoderma
potato infection
Polska
Gliocladium
Penicillium
Pseudomonas
phytopathogen
bacteria
Bacillus
soil-borne fungi
fungi
potato
Źródło:: Journal of Plant Protection Research; 2003, 43, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Polyphenol oxidase and lysozyme mediate induction of systemic resistance in tomato, when a bioelicitor is used
Autorzy:: Goel, N.
Paul, P.K.
Powiązania:: https://bibliotekanauki.pl/articles/66130.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: polyphenol oxidase
lysozyme
Pseudomonas syringae pv.tomato
systemic resistance
tomato
Solanum lycopersicum
crop protection
plant protection
protection method
Opis:: Tomato (Solanum lycopersicum L.) is attacked by Pseudomonas syringae pv. tomato causing heavy damage to the crops. The present study focused on the application of aqueous fruit extracts of neem (Azadirachta indica L.) on a single node of aseptically raised tomato plants. Observations were done, and the changes in the activity and isoenzyme profile of polyphenol oxidase (PPO) and lysozyme, both at the site of treatment as well as away from it, were noted. The results demonstrate that neem extract could significantly induce the activities of both the enzymes as well as upregulate the de novo expression of additional PPO isoenzymes. Induction of systemic acquired resistance (SAR) by natural plant extracts is a potent eco-friendly crop protection method.
Źródło:: Journal of Plant Protection Research; 2015, 55, 4
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Biocontrol of bacterial speck of tomato by aqueous extract of Tagetes erecta
Autorzy:: Goel, N.
Anukrati, K.
Paul, P.K.
Powiązania:: https://bibliotekanauki.pl/articles/65140.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: biological control
bacterial speck
tomato
water extract
Tagetes erecta
plant disease
biopesticide
plant protection
Pseudomonas syringae pv.tomato
Opis:: Biocontrol of plant diseases has emerged as an eco-friendly measure of plant protection and has experienced a lot of devotion in the last two decades. Biocontrol agents include application of microbial agents, their secretion products and natural extracts from different parts of several plants. The present study, therefore, aimed at evaluating the potency of aqueous extracts of Tagetes erecta L. (marigold) in controlling bacterial speck disease in tomato plants. The experimental design consisted of two groups of 50 plants each: group 1 – sprayed with sterile water (control); and group 2 – sprayed with marigold extract. Spraying was performed under aseptic conditions at the third node from the base of each plant. Challenge inoculation with the bacterial speck pathogen Pseudomonas syringae pv. tomato was performed to analyze the disease severity on the test plants. The parameters of study were analysis of alteration in the activity and gene expression of peroxidase (POX), phenyl ammonia lyase (PAL), and polyphenol oxidase (PPO), as well as isoform expression of POX and PPO. The results demonstrated strong inductive effects of the extract on the activity and genes of POX, PAL and PPO. De novo expression of POX and PPO isoforms following marigold extract treatment was also observed. The observations indicate that marigold extract could be a promising biopesticide.
Źródło:: Journal of Plant Protection Research; 2017, 57, 4
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet
Autorzy:: Arzanlou, M.
Mousavi, S.
Bakhshi, M.
Khakvar, R.
Bandehagh, A.
Powiązania:: https://bibliotekanauki.pl/articles/66726.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: inhibitory effect
antagonistic bacteria
Bacillus
bacteria
rhizosphere
sugar-beet plant
Cercospora beticola
leaf spot disease
Pseudomonas
plant disease
Opis:: In the present study, the antagonistic capability of bacterial agents inhabiting the rhizosphere of sugarbeet plants were evaluated against Cercospora beticola Sacc. under laboratory and greenhouse conditions. After preliminary screening using the dual culture method, 14 strains with higher antagonistic capability were selected for further inhibitory assays against C. beticola. Bacterial strains were identified based on the sequence data of the small subunit-rDNA (SSU-rDNA) gene. Based on the SSU sequence data, the identity of bacterial strains were determined as Bacillus (10 strains: RB1, RB2, RB3, RB4, RB5, RB6, RB7, RB8, RB9, RB10), Paenibacillus (two strains: RP1, RP2), Enterobacter (one strain: RE), and Pseudomonas (one strain: RPs). The results obtained in this study showed that in all of the assays (dual culture, volatile and non-volatile metabolites) bacterial antagonists significantly inhibited the growth of C. beticola compared to the control. Bacillus (RB2) showed the highest inhibition rate on C. beticola in all of the assays. Based on the results of the laboratory assays, three bacterial strains RB2 (Bacillus), RPs (Pseudomonas), and RE (Paenibacillus) were selected for greenhouse assays. The experiment was designed based on a completely randomised design (CRD) with the application of antagonists prior to, simultaneously, and after inoculation with C. beticola on sugarbeet leaves. The reduction in disease severity was evaluated seven days after inoculation. The results of greenhouse assays were consistent with the results of laboratory studies. The obtained results showed that bacterial antagonists significantly reduced the disease severity when compared to the control.
Źródło:: Journal of Plant Protection Research; 2016, 56, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Ecological applications of Pseudomonas as a biopesticide to control two-spotted mite Tetranychus urticae: chitinase and HCN production
Autorzy:: Quesssaoui, R.
Bouharroud, R.
Amarraque, A.
Ajerrar, A.
Mayad, E.H.
Chebli, B.
Dadi, M.
Elaini, R.
El Filali, F.
Walters, A.S.
Powiązania:: https://bibliotekanauki.pl/articles/66489.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: biological control
ecological application
Pseudomonas
biopesticide
pest control
two-spotted mite zob.two-spotted spider mite
red spider mite zob.two-spotted spider mite
two-spotted spider mite
Tetranychus urticae
chitinase
hydrogen cyanide
Opis:: The two-spotted spider mite (Tetranychus urticae Koch) is an important pest of many horticultural crops. A study was conducted to evaluate the effect of three fluorescent Pseudomonas isolates obtained from rhizospheric soil of tomato (Solanum lycopersicon) in Agadir, Morocco: Q110B, Q036B and Q172B, as potential biological control agents for T. urticae. Both acaricide and repellent activities were assessed on homogenous adult mites. The acaricidal activity test evaluated five concentrations of bacterial suspensions: 0 (control), 102, 104, 106, 108, and 1010 cfu ⋅ ml–1, while only the 1010 cfu ∙ ml−1 concentration of each bacterium was used for the repellent bioassay. The mortality rate and repellentindex were recorded 24, 48 and 72 h after application. Results indicated that the survival rate of T. urticae was reduced (p ≤ 0.01) by all three bacterial isolates compared to control. Within the 24–72 h time period the mortality rates ranged from 8 to 87%, 16 to 99%, and 13 to 89%, for Q110B, Q036B and Q172B isolates, respectively. The isolate Q036B (LC50 = 0.598 cfu ⋅ ml–1) provided higher mortality rates than Q172B and Q110B with LC50 values of 90,846 and 169,585 cfu ⋅ ml–1, respectively. Repellent activity was also the highest with Pseudomonas Q036B having a 71% repellence index at 48 h after application. Regarding the mechanism of action, all three isolates produced hydrogen cyanide, and exhibited protease and cellulose activities, although only Q036B and Q172B had potential chitinase action. Identification analysis showed that the isolates were either Pseudomonas putida (Q172B) or P. fluorescens (Q110B and Q036B). Our results indicate that the P. fluorescens isolate Q036B is a promising candidate for biological control of T. urticae, and has potential to contribute to an integrated pest management program to control this important pest. Then the fruits produced will be qualified as safe for consumers and the environment. The present work was customized to give support for policy decision makers as an agroecological potential meeting needs of industries and ecological balance.
Źródło:: Journal of Plant Protection Research; 2017, 57, 4
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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