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    Tytuł:
    Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein
    Autorzy:
    Wu, X.
    Xiao, L.
    Peng, B.
    Wang, Y.
    Yang, Z.
    Yao, X.
    Hu, L.
    Lin, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/30963.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis and Modelling on Radiated Noise of a Typical Fishing Boat Measured in Shallow Water Inspired by AQUO Project’s Model
    Autorzy:
    Peng, Z.
    Fan, J.
    Wang, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/177092.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    radiated noise
    South China Sea
    predicted model
    shallow water
    transmission loss
    Opis:
    The shipping noise near channels and ports is an important contribution to the ambient noise level, and the depth of these sites is often less than 100 m. However less attention has been paid to the measurement in shallow water environments (Brooker, Humphrey, 2016). This paper presents extensive measurements made on the URN (underwater radiated noise) of a small fishing boat in the South China Sea with 87 m depth. The URN data showed that the noise below 30 Hz was dominated by the background noise. The transmission loss (TL) was modelled with FEM (finite element method) and ray tracing according to the realistic environmental parameters in situ. The discrepancy between the modelled results and the results using simple law demonstrates both sea surface and bottom have significant effect on TL for the shallow water, especially at low frequencies. Inspired by the modelling methodology in AQUO (Achieve QUieter Oceans) project (Audoly et al., 2015), a predicted model applied to a typical fishing boat was built, which showed that the URN at frequencies below and above 100 Hz was dominated by non-cavitation propeller noise and mechanical noise, respectively. The agreement between predicted results and measured results also demonstrates that this modelling methodology is effective to some extent.
    Źródło:
    Archives of Acoustics; 2018, 43, 2; 263-273
    0137-5075
    Pojawia się w:
    Archives of Acoustics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Maintenance optimization for systems with dependent competing risks using a copula function
    Optymalizacja eksploatacji dla systemów z zależnymi zagrożeniami konkurującymi przy wykorzystaniu funkcji kopuły
    Autorzy:
    Guo, Ch.
    Wang, W.
    Guo, B.
    Peng, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/301712.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
    Tematy:
    zależne ryzyka konkurujące
    funkcja kopuły
    dane symulowane
    degradacja
    zaburzenia losowe
    optymalizacja eksploatacji
    dependent competing risks
    copula function
    simulated data
    degradation
    random shocks
    maintenance optimization
    Opis:
    This paper develops a joint copula reliability model for systems subjected to dependent competing risks caused by two degradation processes and random shocks. The two degradation processes follow gamma processes and the random shocks follow a non-homogeneous Poisson process (NHPP). Their interdependence relationship is modeled by a copula function, which is determined by a two-stage method based on simulated data. It is shown that the proposed model can provide more precise results than the model without considering the dependent relationship. Through the proposed reliability model, two maintenance models are studied and compared. It is found that the inspection cost has significant effects on the choosing of maintenance policy.
    W niniejszej pracy opracowano wspólny model niezawodności z użyciem kopuły dla systemów poddawanych zależnym zagrożeniom konkurującym powodowanym przez dwa procesy degradacji i zaburzenia losowe. Owe dwa procesy degradacji reprezentują typ procesu gamma, podczas gdy zaburzenia losowe są typem niejednorodnego procesu Poissona (non-homogeneous Poisson process - NHPP). Ich związek wzajemnej zależności modelowany jest przy użyciu funkcji kopuły, która jest wyznaczana na podstawie dwuetapowej metody opartej o dane symulowane. Wykazano, iż proponowany model może zapewnić bardziej precyzyjne wyniki niż model, w którym nie ujęto związku zależności. W oparciu o proponowany model niezawodności, badane i porównywane są dwa modele eksploatacji. Stwierdzono, iż koszt przeglądu ma duży wpływ na wybór polityki eksploatacyjnej.
    Źródło:
    Eksploatacja i Niezawodność; 2013, 15, 1; 9-17
    1507-2711
    Pojawia się w:
    Eksploatacja i Niezawodność
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Design and experiments using a spiral-liquid-solid fluidized bed system
    Autorzy:
    Sha, J.
    Liang, L.
    Liu, B.
    Xie, G.
    Peng, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/110520.pdf
    Data publikacji:
    2015
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    liquid-solid fluidized bed
    spiral unit
    coal separation
    water quantity
    Opis:
    Liquid-solid fluidized bed (LSFB) has been widely known and used for separation of coarse coal particles (normally larger than 0.25 mm). The process of separation by LSFB needs fluidization water from the bottom to the top of LSFB. The fluidization water is formed by the water addition at the bottom of the LSFB. Normally the quantity of water addition is very large, which increases the burden of water treatment in coal preparation processes. In this investigation, a spiral unit was introduced into the conventional LSFB and the new separation equipment was named S-LSFB. The spiral unit could provide an upward force for the upward movement of coarse low density coals into the concentrate, and hence the quantity of water addition for fluidization water may be reduced. Samples of 0.5-0.25 mm size fraction coal were used to investigate the difference in separation performance between S-LSFB and LSFB. It was found that the separation performance of S-LSFB was nearly equal to that of LSFB. S-LSFB may be beneficial to coarse coal separation in coal preparation plant since the burden of water treatment can be reduced by the application of S-LSFB.
    Źródło:
    Physicochemical Problems of Mineral Processing; 2015, 51, 2; 427-434
    1643-1049
    2084-4735
    Pojawia się w:
    Physicochemical Problems of Mineral Processing
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synthesis, Characterization and Thermal Decomposition of a New Energetic Salt of 1H,1′H-5,5′-Bistetrazole-1,1′-diol
    Autorzy:
    Xiao, L.
    Jin, B.
    Shang, Y.
    Liu, Q.
    Guo, Z.
    Peng, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/358377.pdf
    Data publikacji:
    2018
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    1,1-dimethylbiguanidium 1H,1′H-5,5′-bistetrazole-1,1′-diolate (MGBTO)
    single crystal
    thermal properties
    energetic properties
    Opis:
    1,1-Dimethylbiguanidium 1H,1′H-5,5′-bistetrazole-1,1′-diolate (MGBTO), a novel nitrogen-rich energetic salt, was synthesized by cation exchange. Its structure was characterized by elemental analysis, FTIR, NMR, DTA and TG-DTG. Single crystal X-ray diffraction analysis revealed that MGBTO was crystallized in the monoclinic space group C2/c. Thermal analysis demonstrated that its thermal stability extended up to 531.1 K. The nonisothermal kinetic and apparent thermodynamic parameters of the exothermic decomposition of MGBTO were determined by the Kissinger and Ozawa methods. Its detonation velocity and detonation pressure were calculated on the basis of the Kamlet-Jacobs equation and were 6342 m·s–1 and 15.78 GPa, respectively. The impact and friction sensitivities of MGBTO were quantified using standard BAM (10 kg drop hammer) procedures. The results revealed that the salt has good mechanical sensitivity (FS > 120 N, IS > 40 J), thus indicating its potential applications as an energetic material.
    Źródło:
    Central European Journal of Energetic Materials; 2018, 15, 3; 405-419
    1733-7178
    Pojawia się w:
    Central European Journal of Energetic Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tests and analyses on mechanical characteristics of dwarf-dense-early major cotton variety stalks
    Autorzy:
    Liang, R.
    Chen, X.
    Zhang, B.
    Peng, X.
    Meng, H.
    Jiang, P.
    Li, J.
    Zhou, P.
    Kan, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2082915.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Instytut Agrofizyki PAN
    Tematy:
    cotton stalk
    dwarf-dense-early
    multiple factors
    mechanical characteristics
    Opis:
    The influence of the variation in water content, sampling location and loading speed on the shearing and across-compression mechanical characteristics of dwarf-denseearly major cotton variety stalks was analysed. The second-order response models of the factors vs. the maximum shear force and critical across-compression failure force were built using the response surface methodology. Besides, the sampling location and water content have a significant influence over the maximum shear force and critical across-compression failure force, especially the sampling location. Both the maximum shear force and critical across-compression failure force reach a theoretical maximum when the water content is 10%, the sampling location is the lower part and the loading speed is 40 mm min-1. When the sampling location is the lower part, optimization is carried out assuming the theoretical minimum of the maximum shear force and critical across-compression failure force as the optimization target. In this case, the loading speed is 20 mm min-1, while the water content is between 30 and 50%. This indicates that the parameter range requires a low power consumption during the crushing of film residue mixtures. The test data can provide some theoretical references for the technical research and equipment development for the crushing of film.
    Źródło:
    International Agrophysics; 2020, 34, 3; 333-342
    0236-8722
    Pojawia się w:
    International Agrophysics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of organic cultivation pattern on tomato production: plant growth characteristics, quality, disease resistance, and soil physical and chemical properties
    Autorzy:
    Feng, X.
    Xu, Y.
    Liu, D.
    Peng, L.
    Dong, J.
    Yao, S.
    Feng, Y.
    Feng, Z.
    Li, F.
    Hu, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/12703546.pdf
    Data publikacji:
    2020
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Opis:
    Tomato (Solanum lycopersicum L.) is one of the world’s most important cultivated vegetable. In the traditional cultivation methods, the excessive use of pesticides and fertilizers leads to an imbalance of nutrient elements in the soil, an increase in pests and diseases and a decrease in vegetable quality and yield. In the face of increasingly serious environmental and food problems, organic agriculture is considered to be an effective solution. In this experiment, the effects of organic cultivation patterns on the growth, quality, disease resistance in tomatoes, and the physical and chemical properties of soil were studied by different treatments. The results showed that the application of effective microorganisms (EM) bio-organic fertilizer in the cultivation process can significantly improve the yield, quality, and antioxidant enzyme activity of tomato. The use of straw mulching was found to significantly increase the growth, chlorophyll content, transpiration rate, and soluble sugar content of tomatoes. The application of EM bio-organic fertilizer or straw mulching significantly increased the activity of antioxidant enzyme and the expression of LeCHI gene in tomato leaves and enhanced tomato resistance to diseases. Organic production practices were found to significantly improve the soil.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 1; 71-84
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Improvements in timber production of Chinese fir (Cunninghamia lanceolata) per unit forest area in China via tree breeding: Status and challenges
    Autorzy:
    Zhou, B.
    Peng, D.
    Zhao, Q.
    Yangnan, S.
    Yang, S.
    Yang, F.
    Qu, G.
    Tang, W.
    Ou, J.
    Xiang, W.
    Xiang, J.
    Deng, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/2077649.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Cunninghamia lanceolata
    genetic improvement
    timber volumes per unit forest area
    wood properties
    proposals
    recommendations
    Opis:
    Chinese fir [Cunninghamia lanceolata (Lamb.) Hook] is a fast-growing tree species that is widely planted for timber production in southern China. Many high-quality breeding systems for Chinese fir have been developed using genetic methods. Here, we systematically review progress in increasing Chinese fir timber volumes per unit forest area in China, using genetic methods. We comprehensively assess the objec- tives, strategies, and primary accomplishments of the first to fourth generations of a Chinese fir breeding program. Furthermore, we propose a genetic improvement program and protocol for Chinese fir, and out- line several recommendations with respect to the main challenges associated with using genetic methods to increase timber yields. Finally, we present a series of proposals to increase Chinese fir timber production in China. This review offers insight into genetic improvements that have increased timber volumes of Chi- nese fir over four generations of breeding, and provides direction for future genetic improvements to wood properties.
    Źródło:
    Dendrobiology; 2020, 83; 43-51
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The distribution of different virulence grass carp reovirus strains in some neglected tissues
    Autorzy:
    Liang, H.R.
    Fu, X.Z.
    Li, N.Q.
    Liu, L.H.
    Lin, Q.
    Li, Y.G.
    Peng, Y.A.
    Huang, Z.B.
    Wu, S.Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/30668.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterization of serum adiponectin and leptin in healthy perinatal dairy cows or cows with ketosis, and their effectson ketosis involved indices
    Autorzy:
    Shen, L.
    Qian, B.
    Xiao, J.
    Zhu, Y.
    Hussain, S.
    Deng, J.
    Peng, G.
    Zuo, Z.
    Zou, L.
    Yu, S.
    Ma, X.
    Zhong, Z.
    Ren, Z.
    Wang, Y.
    Liu, H.
    Zhou, Z.
    Cai, D.
    Hu, Y.
    Zong, X.
    Cao, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087289.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    adiponectin
    dairy cows
    ketosis
    leptin
    perinatal period
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 3; 373-381
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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