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Tytuł:
Influence of magnetic fields on the activity of enzymes: alpha- and beta-amylase and glutathione S-transferase [GST] in wheat plants
Autorzy:
Rochalska, M.
Grabowska, K.
Powiązania:
https://bibliotekanauki.pl/articles/25053.pdf
Data publikacji:
2007
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
wheat plant
magnetic field
glutathione S-transferase
beta-amylase
enzyme
alpha-amylase
magnetic biostimulation
germination
Źródło:
International Agrophysics; 2007, 21, 2
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Content of glycogen and trehalose and activity of alpha-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae
Autorzy:
Zoltowska, K
Lopienska-Biernat, E.
Powiązania:
https://bibliotekanauki.pl/articles/839414.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Galleria mellonella
Steinernema feltiae
insect host
amylase
trehalase
host
parasite
trehalose
glycogen
parasitology
alpha-amylase
nematode
larva
Steinernema affinis
entomopathogenic nematode
Opis:
Introduction. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on metabolism of glycogen and trehalose of the host was studied. Material and methods. Last instar larvae (L₇) of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae. At 6, 12, 18 and 24 h after infection concentrations of trehalose and glycogen as well as activity of trehalase and α-amylase were determined. Results. The content of glycogen was lower in insects infected with S. feltiae than in the controls and animals infected with S. affinis. The content of trehalose was higher in insects from both infected groups than in the controls. Its concentration was slightly higher in larvae infected with S. affinis than in those infected with S. feltiae. The activity of α-amylase after infection with S. affinis was low. It was significantly higher in insects infected with S. feltiae. In animals of both infected groups, following a significant reduction at 6 h, the activity of trehalase remained at a similar level, higher than in the controls. In the paper the effects of infection with (i) different species of entomopathogenic nematodes and (ii) the importance of the developmental stage of the insect-host for changes in its metabolism of glycogen and trehalose were discussed.
Źródło:
Annals of Parasitology; 2006, 52, 2
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Content of glycogen and trehalose and activity of alpha-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae
Autorzy:
Żółtowska, K.
Łopieńska-Biernat, E.
Powiązania:
https://bibliotekanauki.pl/articles/2144330.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Galleria mellonella
Steinernema feltiae
insect host
amylase
trehalase
host
parasite
trehalose
glycogen
parasitology
alpha-amylase
nematode
larva
Steinernema affinis
entomopathogenic nematode
Opis:
Introduction. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on metabolism of glycogen and trehalose of the host was studied. Material and methods. Last instar larvae (L₇) of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae. At 6, 12, 18 and 24 h after infection concentrations of trehalose and glycogen as well as activity of trehalase and α-amylase were determined. Results. The content of glycogen was lower in insects infected with S. feltiae than in the controls and animals infected with S. affinis. The content of trehalose was higher in insects from both infected groups than in the controls. Its concentration was slightly higher in larvae infected with S. affinis than in those infected with S. feltiae. The activity of α-amylase after infection with S. affinis was low. It was significantly higher in insects infected with S. feltiae. In animals of both infected groups, following a significant reduction at 6 h, the activity of trehalase remained at a similar level, higher than in the controls. In the paper the effects of infection with (i) different species of entomopathogenic nematodes and (ii) the importance of the developmental stage of the insect-host for changes in its metabolism of glycogen and trehalose were discussed.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 2; 103-107
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DIPSACUS FULLONUM L. LEAVES AND ROOTS - IDENTIFICATION OF THE COMPONENTS OF THE ESSENTIAL OILS AND ALPHA-AMYLASE INHIBITORY ACTIVITIES OF METHANOLIC EXTRACTS
Autorzy:
Witkowska-Banaszczak, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/895320.pdf
Data publikacji:
2018-08-31
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
essential oil composition
methanolic extract
alpha-amylase inhibition
Opis:
Abstract: Dipsacus fullonum L. syn. D. sylvestris is a biennial plant from the Dipsacaceae family. It grows in Europe, North Africa and Asia Minor. It has been used in folk medicine mainly to treat Lyme disease. This study was undertaken to identify and compare the components of the essential oils from dried and fresh roots, which are usually applied as medicinal agents, as well as those of the essential oils from dried and fresh leaves, used less frequently. The essential oils from the Dipsacus fullonum leaves and roots were analysed by the GC and GC-MS analysis. 18 components were identified in the essential oil from the fresh leaves, whereas 37 were recognised in the oil from the dried leaves; 10 components were identified in the essential oil from the fresh roots and 40 in that from the dried roots. Phytol was the main component in the essential oil from the leaves, whereas n-hexadecanoic acid was predominant in the roots. The α -amylase inhibition has been proven for methanolic extracts of D. fullonum leaves and roots. The strongest activity was demonstrated by the extract from fresh leaves of D. fullonum and showed an effect about one hundred times weaker than acarbose.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 951-957
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Physical and chemical properties of sweet juice produced from hydrolysed acha (Digitaria exilis Stapf) starch using crude amylase from germinated maize
Autorzy:
Abdulquadri, O. Alaka
Rahman, Akinoso
Powiązania:
https://bibliotekanauki.pl/articles/1179002.pdf
Data publikacji:
2017
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Maize amylase
acha
germinative capacity
juice
starch
Opis:
This study investigated the possibility of producing juice by hydrolyzing acha starch using crude amylase from germinated maize. Planted maize was monitored for seven days to determine germinative capacity and amylase activity of the seed. Extracted acha starch was hydrolysed using wet homogenised maize seedlings in an incubator at 62-68 °C for 6-8 hours. Colour, pH, titratable acidity (TTA), °Brix and sensory attribute of the juice were determined using standard method. A commercial malt beverage was used as reference. The maize sample had a mean capacity of 95% while its maximum amylase activity occurred at day 4. The pH, TTA, and °Brix of the juice were obtained as 4.5, 0.85 and 10 respectively. Colour values for L*, a*, and b* were 37.64, 7.6, and 15.3 respectively. Sensory analysis showed no significant difference between the juice and reference sample in terms of taste and colour. However, significant differences were observed in aroma and overall acceptability. Hence, malt beverages can be produced using this method but requires certain additives to impart prevalent aroma associated with malt beverages.
Źródło:
World Scientific News; 2017, 87; 125-135
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Alfa-amylaza granulocytów człowieka
Alfa-amilaza chelovecheskikh granulocitov
Alpha-amylase in granulocytes
Autorzy:
Zakrzewska, I.
Powiązania:
https://bibliotekanauki.pl/articles/2187896.pdf
Data publikacji:
1985
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1985, 21, 4-5; 248-258
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Germination, alpha-, beta-amylase and total dehydrogenase activities of Amaranthus caudatus seeds under water stress in the presence of ethephon or gibberellin A3
Autorzy:
Bialecka, B
Kepczynski, J.
Powiązania:
https://bibliotekanauki.pl/articles/19888.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Amaranthus caudatus
seed
germination
alpha-amylase activity
beta-amylase activity
dehydrogenase activity
water stress
ethylene
gibberellin A3
polyethylene glycol
ethephon
water deficit
laboratory condition
plant
environmental stress
Opis:
Amaranthus caudatus L. seed germination was studied under different levels of water deficit induced by PEG 6000 in laboratory conditions. PEG at osmotic potentials -0.2 to -0.3 MPa at 24°C in darkness delayed germination and reduced final germination percentage. PEG solutions at osmotic potential lower than -0.3 MPa almost totally blocked seed germination. Ethephon was much more effective than GA3 in reversing PEG-caused inhibition of A. caudatus seed germination. PEG decreased α-amylase activity after 14 h incubation. It decreased β-amylase activity after 14 and 20 h, and caused an increase in total dehydrogenase activity only after 20 h of incubation. Unlike GA3, ethephon increased α-amylase activity in seeds after 12 and 14 h of incubation under water deficit. After 20 h of incubation there was no difference in α-amylase activity in any of the treatments. Neither ethephon nor GA3 affected the activity of β-amylase and dehydrogenase.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2010, 52, 1; 7-12
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro α-glucosidase and α-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide
Autorzy:
Subramanian, Rammohan
Asmawi, M
Sadikun, Amirin
Powiązania:
https://bibliotekanauki.pl/articles/1040761.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
α-glucosidase
α-amylase
Andrographis paniculata
andrographolide
peak blood glucose
post prandial hyperglycaemia.
Opis:
There has been an enormous interest in the development of alternative medicines for type 2 diabetes, specifically screening for phytochemicals with the ability to delay or prevent glucose absorption. The goal of the present study was to provide in vitro evidence for potential inhibition of α-glucosidase and α-amylase enzymes, followed by a confirmatory in vivo study on rats to generate a stronger biochemical rationale for further studies on the ethanolic extract of Andrographis paniculata and andrographolide. The extract showed appreciable α-glucosidase inhibitory effect in a concentration-dependent manner (IC50=17.2±0.15 mg/ml) and a weak α-amylase inhibitory activity (IC50=50.9±0.17 mg/ml). Andrographolide demonstrated a similar (IC50=11.0±0.28 mg/ml) α-glucosidase and α-amylase inhibitory activity (IC50=11.3±0.29 mg/ml). The positive in vitro enzyme inhibition tests paved way for confirmatory in vivo studies. The in vivo studies demonstrated that A. paniculata extract significantly (P<0.05) reduced peak blood glucose and area under curve in diabetic rats when challenged with oral administration of starch and sucrose. Further, andrographolide also caused a significant (P<0.05) reduction in peak blood glucose and area under the curve in diabetic rats. Hence α-glucosidase inhibition may possibly be one of the mechanisms for the A. paniculata extract to exert antidiabetic activity and indicates that AP extract can be considered as a potential candidate for the management of type 2 diabetes mellitus.
Źródło:
Acta Biochimica Polonica; 2008, 55, 2; 391-398
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic regulation of alpha-amylase synthesis in rye [Secale cereale L.] grain
Autorzy:
Masojc, P
Stojalowski, S
Lapinski, M
Miazga, D
Powiązania:
https://bibliotekanauki.pl/articles/2047279.pdf
Data publikacji:
1996
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
enzyme synthesis
activity
chromosome
rye
Secale cereale
crossing
alpha-amylase
sprouting line
duplicate gene
addition line
genetic regulation
Opis:
Genetic analysis of two rye interline crosses and a set of wheat/rye chromosomal addition lines was performed to reveal the mechanism underlying wide variation range of alpha-amylase activity in sound grain. The long arm of chromosome 6R was found to be responsible for increased enzyme synthesis during late stages of triticale grain maturation. Only nuclear genes seemed to control alpha-amylase activity, as reciprocal crosses between rye lines showed no maternal effects. Low enzyme activity showed complete dominance over high level of its synthesis. Segregation ratios, observed in F₂ and BC₁ crosses, indicated that recessive alleles at two independent duplicative loci underlie intensive alpha-amylase production.
Źródło:
Journal of Applied Genetics; 1996, 37, 2; 141-152
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Production of amylase by the intestinal microflora of cultured freshwater fishes (Oreochromis niloticus and Clarias gariepinus) rared localy in Calabar, south Nigeria
Autorzy:
Martin, M. F.
Okpo, E. A.
Andy, I. E.
Powiązania:
https://bibliotekanauki.pl/articles/1076792.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Amylase
Clarias gariepinus
Cultured Fresh Water Fishes
Intestinal Microflora
Oreochromis niloticus
Opis:
This study focused on determining the amylase-producing ability of the intestinal microbes in cultured fresh water fishes – Oreochromis niloticus and Clarias gariepinus. The bacterial isolates were identified on the basis of standard cultural, morphological and biochemical characteristics. The amylase production ability of the bacterial isolates was determined using starch agar. The mean viable count of the intestinal microbes ranged from 1.2 × 105 CFU/ml to 7.1 × 105 CFU/ml for tilapias (Oreochromis niloticus) and from 2.0 × 104 CFU/ml to 8.9 × 104 CFU/ml for catfishes (Clarias gariepinus). Staphylococcus and Micrococcus were predominant for both tilapias and catfishes. Out of 24 isolates, 21 were amylase producers. These included the following bacteria genera: Bacillus, Micrococcus, and Staphylococcus. These results strongly suggest that intestinal microbes play a pivotal role in the digestion of starch in cultured freshwater fishes and should be explored for industrial amylase production.
Źródło:
World News of Natural Sciences; 2019, 23; 13-23
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of feed supplement with alfa-amylase and beta-glucanase on ingestive-related biomarkers registered with real-time sensors
Autorzy:
Antanaitis, R.
Anskienė, L.
Televičius, M.
Malašauskienė, D.
Šertvytytė, G.
Stoškus, R.
Meškinytė, E.
Rous, J.
Helm, B.
Powiązania:
https://bibliotekanauki.pl/articles/16626661.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
alfa-amylase
beta-glucanase
reticulorumen
rumination
Opis:
The aim of this study was to investigate the impact of feed supplements with alfa-amylase and beta-glucanase (Optipartum C+ 200) on ingestive-related behaviour biomarkers registered with real-time sensors: rumination behaviours and reticulorumen parameters (pH and temperature). Cows (n=20) in the treatment group (TG) were fed with Optipartum C+ 200 (Enzymes feed supplement: Alfa-Amylase 57 Units; Beta-Glucanase 107 Units) from 21 days before calving until 30 days after calving with a feeding rate of 200 g/cow/day. Cows (n=22) in the control group (CG) were fed a feed ration without feed supplement. Measurements started from 6 days before calving and continued until 21 days after calving. The following indicators were registered: with the RumiWatch System: Rumination time; Eating time; Drinking time; Rumination chews; Eating chews; Drinking gulps; Bolus; Chews per minute; Chews per bolus. With the SmaXtec system: the temperature, pH of the contents of the cows’ reticulorumens, and cows’ walking activity. According to our results, feed supplementation with alfa-amylase and beta-glucanase (Optipartum C+ 200) in the TG group resulted in increases in the following parameters: 9% rumination time and eating time, 19% drinking time, 11% rumination chews, 16% eating chews, 13% number of boluses per rumination, 5% chews per minute and 16% chews per bolus. The rumination time showed a strong, positive relation with rumination chews and bolus indicators in both groups (TG and CG) (p<0.001); while the rumination time in both groups of cows showed an opposite direction and was negatively related to eating time and eating chews (p<0.05). We found a 1.28 % lower reticulorumen pH and a 0.64 % lower reticulorumen temperature in cows fed with the supplement compared with cows in the control group. Cows in TG were 8.80% more active than those in the CG group. For improvement of ingestive-related behaviour we suggest adding a feed supplement with alfa-amylase and beta-glucanase (Optipartum C+ 200).
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 535-546
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Physical factors affecting the production of amylase from Bacillus species isolated from natural environment
Wpływ czynników fizycznych na aktywność amylolityczną Bacillus sp. wyizolowanych ze środowiska naturalnego
Autorzy:
Grata, K.
Nabrdalik, M.
Powiązania:
https://bibliotekanauki.pl/articles/389748.pdf
Data publikacji:
2014
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
Bacillus cereus
Bacillus mycoides
amylase
physical factor
amylazy
czynniki fizyczne
Opis:
The influence of different factors on amylolytic activity Bacillus cereus (2 strains) and Bacillus mycoides (2 strains), isolated from soil samples and water of Turawa Lake has been studied. Effect of physiological (pH of reaction mixture in the range of 4.0–8.0, temperature of reaction from 30 to 60 oC) and nutritional parameters (the carbon sources and they concentration in medium) were examined spectophotometricaly for their effect on amylase production. The results obtained showed, that in view of all studied factors the least favorable value of pH was 5.0, while in most cases the most favourable for the process of amylase production were following values 7.0 and 8.0. Moreover, it has been stated that the lowest amount of amylase was noted at 30 oC, and the highest at 60 oC. In case of amylolytic activity, in view of all temperatures the most effective strain was B. mycoides A134 whereas the least B. mycoides G3. The best results of amylase production have been achieved for all remaining strains in two cases – on maltose and glucose medium at all tested temperatures. Additionally, soil occurring strains preferred lower concentration of potato starch (ie 1 %) when compared with water strains which favoured higher concentration (5 %).
Celem badań była ocena wpływu różnych czynników na aktywność amylolityczną Bacillus cereus (2 szczepy) i Bacillus mycoides (2 szczepy) wyizolowanych z gleby i Jeziora Turawskiego. Metodą spektrofotometryczną badano wpływ pH mieszaniny reakcyjnej w zakresie od 5.0 do 8.0 i temperatury w zakresie od 30 oC do 60 oC oraz źródła węgla i jego koncentracji w podłożu na poziom produkowanych amylaz. Stwierdzono, iż najgorszą wartością pH do syntezy amylaz było 5.0, natomiast najlepszą, w większości przypadków pH 7.0. Uwzględniając zakres badanych temperatur, najwyższą aktywność uzyskano w temp. 60 oC, natomiast najniższą w temp. 30 oC dla wszystkich testowanych szczepów. Najbardziej aktywnym szczepem był B. mycoides A 134, natomiast najmniej B. mycoides G3.
Źródło:
Ecological Chemistry and Engineering. A; 2014, 21, 1; 51-60
1898-6188
2084-4530
Pojawia się w:
Ecological Chemistry and Engineering. A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Interaction between larval alpha-amylase of the tomato leaf miner, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) and proteinaceous extracts from plant seeds
Autorzy:
Esmaeily, M.
Bandani, A.R.
Powiązania:
https://bibliotekanauki.pl/articles/65574.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
interaction
larva
alpha-amylase
tomato leafminer
Tuta absoluta
Lepidoptera
Gelechiidae
plant extract
seed
plant seed
Opis:
The tomato leaf miner, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae), is one of the most destructive pest of solanaceae and it prefers tomato (Solanum lycopersicum L.). The aim of the current study was to investigate the effects of a wide range of seed proteinaceous extracts from different plant families against T. absoluta α-amylase activity. The effect of pH on the inhibitory activity of seed extracts showed that seed extracts of amaranth along with a wheat cultivar (Alvand, Aflak, Sarvdasht, Alborz, and Kavir) produced more than a 50% inhibition of the insect amylase. Aflak wheat seed extract at 10 μg, inhibited 81% of the insect amylase. This percent was the highest inhibition achieved. The other proteinaceous seed extracts had a lower effect on the enzymatic activity. Probit analysis showed that Aflak, Kavir, Alborz, Alvand, Sarvdasht, and amaranth inhibited the amylase activity with an I50 of 1.94, 3.24, 3.46, 3.31, 4.97, and 15.39 μg, respectively. The effect of pH on the inhibition of the α-amylase showed the highest inhibition of Amaranth and wheat, at a pH value of 8.0, which corresponds to the pH of the insect’s gut. Gel electrophoresis assays confirmed the spectrophotometric assays showing that the α-amylase of the insect gut was affected by the presence of the seed extracts. In the gel assay, a high concentration (14 μg protein) of amaranth proteinaceous seed extract greatly decreased the intensity of the α-amylase band. A high concentration of the Aflak wheat cultivar (10 μg protein) caused the disappearance of the amylase band in the gel. Thus, it is concluded that the physiochemical environment of the insect gut affects the interaction between digestive α-amylase and the metabolites. The experiments showed that seed proteinaceous extracts from non-host plant species, produced more inhibition of the insect amylase when compared to the host plant species. It appears that with evolution, adaptation took place so that insect/s could overcome the plant metabolites.
Źródło:
Journal of Plant Protection Research; 2015, 55, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Starch-iodine assay method underestimates alpha-amylase inhibitory potential of antioxidative compounds and extracts
Autorzy:
Ononamadu, C.J.
Ezeigwe, O.C.
Owolarafe, T.A.
Ihegboro, G.O.
Lawal, T.A.
Salawu, K.
Umeoguaju, U.F.
Aminu, I.
Powiązania:
https://bibliotekanauki.pl/articles/2096980.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
α-amylase inhibition
starch-iodine
diabetes mellitus
limitations
decolorization
Opis:
Starch-iodine assay method for the determination of α-amylase activity is also used in screening extracts for α-amylase inhibitors. However, there are indications that this method may not be appropriate for screening some classes of compounds or plant extracts. The present study investigated the limitation(s) of this method in screening plant extracts/compounds for α-amylase inhibition. A crude methanol extract (CR) of Dacryodes edulis, its solvent fractions (ethyl acetate (EA), aqueous methanol (AM), and hexane (HX)), quercetin (QC), and benzoic acid (BA) were used for this study. The phytochemical content and antioxidant activity were screened spectrophotometrically. α-Amylase inhibition (expressed in percentage and as IC50) was determined by starch-iodine method approach I and II (ST-ID I and ST-ID II, respectively) and dinitrosalicylic acid (DNSA) as the control method. The results showed that the extracts/compounds (AM, EA, and QC) with significantly high polyphenolic content, antioxidant activity, and starch-iodine complex decolorization effect yielded contrary results of α-amylase inhibition when the results of ST-ID I and II methods were compared to that of the DNSA method. The other test samples (CR, HX, and BA) yielded similar results for all the three methods. The result also showed the decolorization (%) of starch-iodine complex by the test samples correlated significantly (r = 0.877, P < 0.05) with DPPH reduction (%). In conclusion, the present study showed that the starch-iodine method is not appropriate for screening antioxidative extracts/compounds for α-amylase inhibitors – they decolorize the assay reagent in a manner similar to DPPH reduction and hence confound the result.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 1; 45-54
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
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