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Wyświetlanie 1-15 z 17
Tytuł:
Immunohistochemical evaluation of expression of heat shock proteins HSP70 and HSP90 in mammary gland neoplasms in bitches
Autorzy:
Badowska-Kozakiewicz, A.M.
Malicka, E.
Powiązania:
https://bibliotekanauki.pl/articles/31330.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Heat shock proteins have essential roles in a number of pathophysiologic conditions including carcinogenesis and represent a group of novel molecular markers in cancer management. The aim of this study was to investigate heat shock protein expression in correlation with other neoplasm traits such as: histological type, differentiation grade, proliferative activity, estrogenic receptor expression, and cyclooxygenase-2 and p53 proteins. Material for the investigation comprised 133 tumors of the mammary gland collected from bitches. In total 14 adenomas, 66 complex carcinomas, 47 simple carcinomas and 6 solid carcinomas were collected. Evaluations were conducted with histopathological and immunohistochemical methods using suitable antibodies. Expression of heat shock protein 70 was observed in all types of evaluated neoplasms. A higher average number of cells undergoing expression of heat shock protein 70, which was statistically insignificant, was established in complex and simple cancers and in cancers with the 1st and the 2nd degree of histological malignancy. Expression of heat shock protein 90 was observed in all studied neoplasms; it was very insignificant in adenomas, compared to cancers, and the highest expression was established in the solid cancers, as well as in cancers with the 2nd degree of histological malignancy. This high expression of heat shock protein 90 was correlated with proliferative activity. The results suggest that heat shock protein 90 is involved in canine mammary gland carcinogenesis. The results also suggest that heat shock protein 90 may be a prognostic factor, but this requires detailed clinical confirmation.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nowotwory gruczołu sutkowego u suk - model badań raka piersi u kobiet
Canine mammary tumors - a model for studying breast cancer in women
Autorzy:
Dolka, I.
Motyl, T.
Malicka, E.
Sapierzynski, R.
Powiązania:
https://bibliotekanauki.pl/articles/859178.pdf
Data publikacji:
2010
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
onkologia
psy
suki
nowotwory gruczolu sutkowego
czlowiek
nowotwory piersi
modele zwierzece
czynniki prognostyczne
markery nowotworowe
markery immunohistochemiczne
Źródło:
Życie Weterynaryjne; 2010, 85, 12
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proliferation activity in canine lymphomas
Autorzy:
Sokolowska, J.
Micun, J.
Malicka, E.
Lechowski, R.
Powiązania:
https://bibliotekanauki.pl/articles/31622.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Forty five lymphomas, 14 of T cell origin, 28 of B cell and 3 with null-cell phenotype, were included in this study. Tumors were classified according to the updated Kiel classification system adapted to canine lymphomas. The percentage of Ki67+ cells and mitotic index (MI) were estimated in each specimen. Most of lymphomas (39 of 45) had high proliferation activity. Among them in 27 cases 50-70% of lymphoma cells expressed Ki67, the highest Ki67 expression (>70% Ki67+ cells) was identified less frequently, in 12 cases. Moderate Ki67 expression (20-50% positive cells) was observed in 5 cases, only one tumor had low Ki67 expression (<20% positive cells). Lower percentage of Ki67+ cells was usually accompanied with lower MI. The mean MI values in discussed groups differed significantly. Mean MI value was also significantly higher in T cell than in B cell lymphomas (4.30 vs. 3.33). Moreover, high positive correlation between the expression of Ki67 and MI was found (r = 0.668; P ≤ 0.001). In T-cell tumors the correlation was very high (r = 0.83; P ≤ 0.001) and in B-cell lymphomas the correlation was high (r = 0.61; P ≤ 0.001). There were also differences between mean MI values in the lymphomas of different morphological subtypes, but in some of them high variations in the range of MI values were identified and wide overlaps of MI between individual cases from different subtypes were observed. Because of differences in the proliferation activity in single cases of the same subtype of lymphoma, the proliferation activity assessment may be helpful to chose appropriate scheme of treatment and should be commonly performed during routine histopathological diagnosis of canine lymphomas.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Immunohistochemical study of expression of immunoglobulins in canine B-cell lymphomas
Autorzy:
Sokolowska, J.
Micun, J.
Zabielska, K.
Malicka, E.
Lechowski, R.
Powiązania:
https://bibliotekanauki.pl/articles/31685.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
immunohistochemistry
expression
immunoglobulin
dog
B cell
lymphoma
tumour
neoplastic cell
immunoglobulin light chain
cancer
Opis:
Nineteen canine lymphomas were included in this study. Tumors were classified according to the updated Kiel classification adapted for canine lymphomas by Fournel-Fleury et al. Immunoglobulin light chains (κ and λ) and IgM and IgG expression were determined by immunohistochemical method. In all examined cases neoplastic cells were positive for one of the immunoglobulin light chains. Expression of λ light chains and κ light chains was observed in 18/19 and 1/19 tumors, respectively. In the majority of neoplastic cells in each examined specimen this reaction had a membranous pattern (sκ/sλ). In all examined cases the presence of immunoglobulin light chains was also observed in the cytoplasm of some neoplastic cells (cκ/cλ). These cells were usally rare and never constituted a dominant population. The expression of immunoglobulin was found in 13/19 cases. Most lymphomas were sIgM positive (11/13 cases). In one case expression of IgG was found, and in another lymphoma two populations of neoplastic cells with different expression of examined immunoglobulins (cells with IgM+ and IgG+ phenotypes) were observed. The reaction also had a membranous pattern. The cells containing cytoplasmic immunoglobulins were rare, and in most cases were of the same type as the surface immunoglobulins. Our study has confirmed that canine lymphomas are a monoclonal proliferation of B-cells usually expressing immunoglobulin λ light chains and that the vast majority of tumors deriving from B-cells express IgM. Our study also indicates a possibility of occurence of biclonal lymphomas in canine species.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electrical and Magnetic Characterization οf $ZnCr_{2-x}V_{x}Se_{4}$ Spinel Semiconductors
Autorzy:
Duda, H.
Malicka, E.
Groń, T.
Pacyna, A.
Kusz, J.
Powiązania:
https://bibliotekanauki.pl/articles/1791367.pdf
Data publikacji:
2009-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
72.80.Jc
75.50.Pp
75.50.Dd
Opis:
Single crystals of $ZnCr_{2}Se_{4}$ spinel doped with vanadium were prepared by chemical vapour transport. The chemical compositions of three crystals have been determined by X-ray diffraction. The structure refinement using the SHELXL-93 program system determine the cation distribution in the system as $ZnCr_{2-x}V_{x}Se_{4}$. For x values equal to 0.03, 0.1 and 0.13 the observed symmetry was cubic, space group Fd3m. Based on the structural data, influence of the V ions on the magnetic and electrical properties has been analyzed.
Źródło:
Acta Physica Polonica A; 2009, 116, 5; 962-963
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ferromagnetic Order in Single-Crystalline (Cd$\text{}_{x}$Al$\text{}_{y}$)[Cr$\text{}_{2}$]Se$\text{}_{z}$ Semiconductors
Autorzy:
Duda, H.
Rduch, P.
Malicka, E.
Groń, T.
Kusz, J.
Powiązania:
https://bibliotekanauki.pl/articles/2048125.pdf
Data publikacji:
2011-05
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
72.80.Jc
75.50.Pp
75.50.Dd
Opis:
Measurements of the magnetic properties, the electrical conductivity and the Seebeck effect were performed on single crystals (Cd$\text{}_{x}$Al$\text{}_{y}$)[Cr$\text{}_{2}$]Se$\text{}_{z}$ between 77 and 300 K. All samples have a ferromagnetic order with the Curie temperature of 130 K and the paramagnetic Curie-Weiss temperature of 155 K. Both these temperatures do not depend significantly on the Al substitution. The electrical conductivity of single crystals CdCr$\text{}_{2}$Se$\text{}_{4}$ doped with Al was p-type and showed the change of log σ versus 1/T slope above 150 K. This fact is interpreted as being due to the onset of impurity conduction and structural defects.
Źródło:
Acta Physica Polonica A; 2011, 119, 5; 702-704
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Relationship between receptors for insulin-like growth factor - I, steroid hormones and apoptosis-associated proteins in canine mammary tumors
Autorzy:
Dolka, I.
Motyl, T.
Malicka, E.
Sapierzynski, R.
Fabisiak, M.
Powiązania:
https://bibliotekanauki.pl/articles/32455.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
relationship
receptor
insulin-like growth factor
steroid hormone
apoptosis-associated protein
dog
mammary tumour
cancer
Opis:
In the veterinary literature there are few data concerning the expression of insulin-like growth factor type I (IGF-IR) in the canine mammary gland tumors. The aim of the present study was the evaluation of IGF-IR expression and its correlation to the expression of estrogen receptor α (ERα) and progesterone receptor (PR), proteins: Bcl-2, Bax, p53 in canine mammary gland tumors, and also a correlation with other features: bitch’s age, tumor diameter, histologic type of tumor, degree of histologic malignancy, proliferate activity. The study was done on 112 epithelial neoplasms: 21 (19%) were adenoma, 38 (34%) complex carcinoma (adenocarcinoma), 47 (42%) simple carcinoma (adenocarcinoma) and 6 (5%) solid carcinoma. Histochemistry and immunohistochemistry methods were employed. It was shown that more common and/or higher IGF-IR expression in cells of canine mammary gland tumors was related to the histologic type of cancer of worse prognostic (solid and simple carcinoma), high histologic degree of malignancy (IIIo) but the statistical analysis did not reveal any significant differences. We observed the high degree of IGF-IR expression in tumors which displayed the high ERα and PR expression. These results suggest the involvement of IGF-IR in the development of hormonosensitive canine mammary tumors. Additionally, the significant positive correlation between expression of IGF-IR and p53, Bax was found. Our study provides some evidence that interactions exist between the IGF-IR and these apoptosis-associated proteins may contribute to the development and progression of canine mammary gland tumors. These results require further investigations.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hopkinson-Like Effect in Single-Crystalline $CdCr_2Se_4$ and $Cd[Cr_{1.89}Ti_{0.08}]Se_4$
Autorzy:
Malicka, E.
Groń, T.
Pacyna, A.
Duda, H.
Krok-Kowalski, J.
Powiązania:
https://bibliotekanauki.pl/articles/1489913.pdf
Data publikacji:
2012-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
72.80.Jc
75.50.Pp
75.50.Dd
Opis:
The static (dc) and dynamic (ac) magnetic measurements of $CdCr_2Se_4$ and $Cd[Cr_{1.89}Ti_{0.08}]Se_4$ showed their ferromagnetic properties with a Curie temperature $T_{C}$ ≈ 130 K and revealed on the real component of ac susceptibility curve, the peaks near $T_{C}$ at 200 Oe, 450 Oe and 1 kOe, characteristic for the Hopkinson ones. The meaningful reduction of saturation moment to 4.73 $μ_{B}$/f.u. for $Cd[Cr_{1.89}Ti_{0.08}]Se_4$ suggests the diamagnetic configuration of Ti ions, which dilutes the ferromagnetic sublattice of Cr ones and causes reducing of the energy losses visible on the imaginary components of ac susceptibility curve. Close for zero values of higher susceptibility harmonics above $T_{C}$ are pointing out to the lack of the spin fluctuations in the paramagnetic state.
Źródło:
Acta Physica Polonica A; 2012, 121, 3; 690-693
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Temperature on Critical Fields in $Zn_{x}Sb_{y}Cr_{z}Se_{4}$
Autorzy:
Malicka, E.
Groń, T.
Duda, H.
Pacyna, A.
Krok-Kowalski, J.
Powiązania:
https://bibliotekanauki.pl/articles/1791371.pdf
Data publikacji:
2009-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
75.50.Pp
75.30.Kz
75.30.Et
Opis:
The electrical and complex ac dynamic magnetic susceptibility measurements were used to study an influence of temperature on critical fields in single-crystalline $Zn_{x}Sb_{y}Cr_{z}Se_{4}$ spinel system with y = 0.11, 0.16 and 0.20. The p-type conduction and a shift both of the Néel temperature $T_{N}$ to lower temperatures and a susceptibility peak at $T_{m}$ in the paramagnetic region - to higher temperatures were established. Below $T_{N}$ the magnetic field dependence of susceptibility, $χ_{ac}(H)$, shows two peaks. First peak at the critical field $H_{c1}$ slightly decreases with temperature and remains almost constant as Sb content increases. Second peak at the critical field $H_{c2}$ drops rapidly with temperature and remains almost unchanged as Sb content increases. At $T_{N}$ both critical fields disappear.
Źródło:
Acta Physica Polonica A; 2009, 116, 5; 964-966
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Histopathology in diagnosis of broiler chicken and layer diseases – review of cases 1999-2010
Autorzy:
Dolka, I.
Sapierzynski, R.
Bielecki, W.
Malicka, E.
Zbikowski, A.
Szeleszczuk, P.
Powiązania:
https://bibliotekanauki.pl/articles/32185.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to estimate the prevalence of histopathological lesions in the different organs in relation to the commercial-type and the age of birds (i.e. broiler chickens and layers). During the period 1999-2010 a total of 189 cases was submitted to the Division of Animal Pathomorphology, Department of Pathology and Veterinary Diagnostics at WULS. Most cases were found in broiler chickens (66.7%). The majority of the histopathological lesions were detected in the liver and lymphoid organs. In of 29% cases of hepatic injury pathognomonic lesions associated with inclusion body hepatitis (IBH) were found. The mean age of birds was 23 days. Among IBH cases proventriculitis (58%) was more often found than gizzard lesions (25.8%). Interestingly, we noted some intranuclear inclusions in the epithelial cells within the proventriculus. A low percentage of histopathological evidence of infectious bursal disease (IBD) was reported in chickens. The gastrointestinal tract was the second most frequent predilection site for histopathological lesions. Histopathological findings within the heart and lungs were less common and were more often seen in the upper respiratory tract. Cases of infectious laryngotracheitis (ILT) were registered in broiler chickens (3.2%, mean age 37 days) and in layers (4.8%; mean age 196 days). Lesions associated with Marek’s disease, avian leukosis and fowl pox were recognized only in layers, respectively in 3.2% (mean age 176 days), 1.6% (mean age 205 days) and 1.1% (mean age 196 days) of all cases. Avian encephalomyelitis (AE) was noted only in 0.5% of all cases.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Critical Behaviour of the Mean-Field Ferromagnet $Cu_{1.02}[Cr_{1.77}Ti_{0.24}]Se_4$
Autorzy:
Krok-Kowalski, J.
Władarz, G.
Rduch, P.
Malicka, E.
Groń, T.
Guzik, A.
Powiązania:
https://bibliotekanauki.pl/articles/1409626.pdf
Data publikacji:
2012-12
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
75.40.Cx
75.50.Dd
64.60.Ht
Opis:
Magnetization, M, and susceptibility, χ, measurements showed both strong lowering of magnetic moment in comparison with $CuCr_2Se_4$ matrix and zero-field-cooling-field-cooling susceptibility splitting characteristic for the spin-glass behaviour. Isothermal magnetization curves, M(H), easy saturate and large values both of the Curie $T_{C}$=253 K and Curie-Weiss θ=283.5 K temperatures indicate the ferromagnetic order which coexists with the spin-glass state. The critical behaviour investigated around the paramagnetic-ferromagnetic phase transition revealed that the values of critical exponents are close to those predicted by the mean field model for long-range magnetic interactions.
Źródło:
Acta Physica Polonica A; 2012, 122, 6; 1102-1104
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electrical and Magnetic Studies of the $Cd_{x}Cr_{y}V_{z}Se_{4}$ Spinels
Autorzy:
Groń, T.
Duda, H.
Malicka, E.
Zawisza, B.
Krok-Kowalski, J.
Pacyna, A.
Powiązania:
https://bibliotekanauki.pl/articles/1791407.pdf
Data publikacji:
2009-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
72.80.Jc
75.50.Pp
75.50.Dd
Opis:
The electrical and magnetic investigations carried out on the polycrystalline spinels with the general chemical formula $Cd_{x}Cr_{y}V_{z}Se_{4}$ (where z = 0.06, 0.12, 0.24, and 0.31) revealed semiconducting and ferromagnetic properties with a Curie temperature of 127.5 K. A step-like structure of the electrical conductivity, σ(T), was observed for the polycrystal containing 6% V ions in the octahedral sites while the remaining samples showed a typical Arrhenius behaviour of σ(T). These effects are interpreted in terms of ferromagnetic spin clusters which finally dissolve on going towards the paramagnetic limit including non-stoichiometry.
Źródło:
Acta Physica Polonica A; 2009, 116, 5; 969-970
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Specific Heat and Magnetic Properties of Single-Crystalline $(Zn_{0.925}In_{0.054})[Cr_{1.84}In_{0.152}]Se_4$ Semiconductor
Autorzy:
Malicka, E.
Groń, T.
Pacyna, A.
Ślebarski, A.
Goraus, J.
Gągor, A.
Mydlarz, T.
Powiązania:
https://bibliotekanauki.pl/articles/1409656.pdf
Data publikacji:
2012-12
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
65.40.Ba
75.50.Ee
75.50.Pp
Opis:
An antiferromagnetic order with a Néel temperature $T_{N}$ = 17.5 K, a strong ferromagnetic exchange evidenced by a positive Curie-Weiss temperature $\Theta_{CW}$ = 77.3 K, the fuzzy peaks in the real component of susceptibility χ'(T) and the disappearance of the second critical field were established. The curvature of specific heat C(T) and C(T)/T in surrounding of $T_{N}$ indicated a broad peak, characteristic for the system with inhomogeneous magnetic state (spin-glass-like phase). The calculated magnetic entropy showed the value of S(T) ≈ 1 J/(mol K) which is extremely small; i.e., much lower than the magnetic contribution Rln(2S + 1) = 11.52 J/(mol K) calculated for the spin 3/2.
Źródło:
Acta Physica Polonica A; 2012, 122, 6; 1108-1110
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mictomagnetic Order in Cd$\text{}_{0.87}$Cr$\text{}_{1.93}$V$\text{}_{0.06}$Se$\text{}_{4}$ Semiconductor
Autorzy:
Groń, T.
Malicka, E.
Zawisza, B.
Duda, H.
Krok-Kowalski, J.
Pacyna, A. W.
Powiązania:
https://bibliotekanauki.pl/articles/2048136.pdf
Data publikacji:
2011-05
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
72.80.Jc
75.50.Pp
75.50.Dd
Opis:
The complex ac dynamic magnetic susceptibility was used to study the mictomagnetic-like behavior in polycrystalline Cd$\text{}_{0.87}$Cr$\text{}_{1.93}$V$\text{}_{0.06}$Se$\text{}_{4}$ spinel. The temperature dependences of the zero field in-phase (real part) and out-of-phase (imaginary part) components of fundamental susceptibility measured at the oscillating field H$\text{}_{ac}$ = 0.5 Oe and at the constant frequency of 125 Hz are characteristic for the mictomagnetic order. These results well correlate with the anomalies in the second and third harmonics of the ac susceptibility indicating the cluster glass.
Źródło:
Acta Physica Polonica A; 2011, 119, 5; 714-716
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
cDNA fingerprint from the hepatopancreatic glands of pond snails (Lymnaea stagnalis) exposed to benzo[a]pyrene
Autorzy:
Woźny, M.
Kowal, M.
Ciesielski, S.
Florczyk, M.
Wiśniewski, R.
Malicka, E.
Brzuzan, P.
Powiązania:
https://bibliotekanauki.pl/articles/363204.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
bioindicator
differential display
expressed sequence tags
polycyclic aromatic hydrocarbons
bioindykator
obraz zróżnicowanej ekspresji
ekspresja fragmentów sekwencji
policykliczne węglowodory aromatyczne
Opis:
Identification of differentially expressed genes that could be potentially used as biomarkers of PAH exposure of common invertebrate animal (like freshwater snail) would be a valuable resource for investigators interested in toxicology and biomonitoring of aquatic environments. Therefore, the aim of this research was to investigate effects of waterborne benzo[a]pyrene (B[a]P) exposure on mRNA expression in the pond snail’s (Lymnaea stagnalis) hepatopancreatic gland. Toward this end, mature individuals of pond snail (L. stagnalis) were treated with 50µM B[a]P solution in a short 36h static exposure test. Differential Display PCR (DD-PCR) was used to generate a unique cDNA fingerprint of genes that were differentially expressed in the tissues of exposed and unexposed snails. To assess the putative identity of the isolated cDNA amplicons (ESTs), BLAST queries were performed to find similarities in their nucleotide sequence. Real-Time qPCR analysis was used to verify the DD-PCR expression profile. Finally, an additional independent exposure study, including higher dose of B[a]P (100µM), was conducted to validate the expression of selected ESTs. BLAST revealed that only 3 out of 9 isolated ESTs had meaningful information on their putative nucleotide sequence identity. The highest similarity was scored for EST-A1, identified as the transcript of UAP-like protein, found to be up-regulated after B[a]P exposure. The original expression pattern that was observed in DD-PCR step was coherent with results of the qPCR verification for 3 out of 5 analyzed ESTs. However, changes in the ESTs expression were modest and the treatment with B[a]P resulted in significant down-regulation for only 1 unidentified fragment (EST-G42, almost 2-fold; p<0.05) when compared to untreated snails. Although no significant changes were observed for EST-A1 and EST-G42 in the validation study, their expression pattern was consistent with that obtained from DD-PCR. Surprisingly, EST-C5 remained in contrast to the DD-PCR part, but it showed significant down-regulation in group of snails exposed to 100µM B[a]P (3.5-fold; p<0.05). The obtained results show that diverse genes may be involved in the molecular response of the pond snail’s hepatopancreas to treatment with B[a]P. However, further research is needed to confirm the utility of the discovered EST as PAH biomarkers in biomonitoring practices with L. stagnalis as bioindicator species.
Źródło:
Environmental Biotechnology; 2014, 10, 1; 8-17
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
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