- Tytuł:
- Developmental expression and hormonal regulation of male-specific yellow protein mRNA in adults of the desert locust, Schistocerca gregaria
- Autorzy:
-
Begum, M.
Rahman, M.M.
Huybrechts, R.
De Loof, A. - Powiązania:
- https://bibliotekanauki.pl/articles/55232.pdf
- Data publikacji:
- 2008
- Wydawca:
- Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
- Tematy:
-
Schistocerca gregaria
yellow protein
phase transition
juvenile hormone
polymorphism
body colour
adult
yellow colouration
insulin
male
sex determination
insect
hormonal regulation
sex hormone
desert locust - Opis:
- Adult males of Schistocerca gregaria turn yellow when they become sexually mature. This is due to the deposition in the cuticle of a male-specific Yellow Protein (YP), of which the amino acid sequence is known. Yellowing only happens in crowd-reared (gregarious) males, and results from the deposition of a specific ‘Yellow Protein’. If individual males (solitarious) are isolated after the adult emergence, they become sexually mature but they do not turn yellow. On the basis of a partial YP-mRNA sequence, we established a reverse transcriptase polymerase chain reaction (RT-PCR) assay to study the developmental expression of YP in crowd-reared males, isolated-reared males and females. In crowd-reared adult males the transcription of YP gene started from day 5 on, and reached a maximum at day 12. The effects of juvenile hormone (JH), insulin (bovine), corazonin, ecdysone and 20 0H-ecdysone (20E) on the regulation of YP-mRNA synthesis were also investigated. JH made the cuticle turn yellow and, as shown by RT-PCR, YP-mRNA was induced. The effect of 100 μg JHIII was stronger than that of 10 μg. Insulin was only effective in inducing YP-mRNA synthesis at high dose (19 μg) and after more days (18 d). Corazonin and 20E made the cuticle turn black, but no YP-mRNA synthesis was observed. Ecdysone (10 and 100 μg) showed no effect on body coloration and YP-mRNA. Thus, JH was found to be the most potent inducer among the hormones tested.
- Źródło:
-
Pestycydy; 2008, 1-2; 35-41
0208-8703 - Pojawia się w:
- Pestycydy
- Dostawca treści:
- Biblioteka Nauki
Artykuł