Temat: whole blood - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Reduction of oxidative stress in adjuvant arthritis. Comparison of efficacy of two pyridoindoles: stobadine dipalmitate and SMe1.2HCl
Autorzy:: Ponist, Silvester
Mihalova, Danica
Jancinova, Viera
Snirc, Vladimír
Ondrejickova, Olga
Mascia, Cinzia
Poli, Giuseppe
Stancikova, Maria
Nosal, Radomir
Bauerová, Katarína
Powiązania:: https://bibliotekanauki.pl/articles/1040407.pdf
Data publikacji:: 2010
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: whole blood chemiluminescence
inflammation
pyridoindoles
HNE- and MDA-protein adducts
neutrophil
arthritis
oxidative stress
Opis:: The aim of this study was to evaluate the therapeutic potential of oxidative stress (OS) reduction by using pyridoindole (PI) antioxidants in adjuvant arthritis (AA). The substances tested were stobadine dipalmitate (STB) and SMe1. AA was used as animal model. The experiments included healthy animals, control arthritic animals and arthritic animals with administration of PI in the oral daily dose of 15 mg/kg b.m during 28 experimental days. The rats were sacrificed on day 28. Clinical and biochemical parameters were determined. The effect of PI administration was evaluated on the basis of the following parameters: (a) arthritis (volume of hind paws - HPW, change of animal body mass - CBM), (b) OS (chemiluminescence of whole blood - CWB, levels of thiobarbituric acid reacting substance - TBARS and of HNE- and MDA-protein adducts in plasma and activity of γ-glutamyltransferase (GGT) in hind paw joint homogenates). The PI studied significantly increased the CBM of animals and corrected the HPW. STB also significantly decreased the activity of GGT in joint homogenates. SMe1 was more effective in decreasing plasmatic TBARS levels, but STB was more effective in reducing plasmatic HNE- and MDA-protein adducts. The assay for HNE- and MDA-adducts in plasma as a function of time was applied for the first time in AA. STB markedly decreased spontaneous and PMA-stimulated CWB and reduced neutrophil count. In summary, STB was more effective than SMe1 in reducing OS in AA. Our results showed that the reduction of OS in arthritis also corrected the clinical manifestations of the disease.
Źródło:: Acta Biochimica Polonica; 2010, 57, 2; 223-228
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Badanie nanocząstek magnetytu w pełnej krwi ludzkiej za pomocą elektronowego rezonansu paramagnetycznego
A study of magnetite nanoparticles in whole human blood by means of electron paramagnetic resonance
Autorzy:: Kubiak, T.
Krzyminiewski, R.
Dobosz, B.
Schroeder, G.
Kurczewska, J.
Hałupka-Bryl, M.
Powiązania:: https://bibliotekanauki.pl/articles/262101.pdf
Data publikacji:: 2015
Wydawca:: Politechnika Wrocławska. Wydział Podstawowych Problemów Techniki. Katedra Inżynierii Biomedycznej
Tematy:: nanocząstka magnetytu
EPR
ludzka krew pełna
magnetite nanoparticles
whole human blood
Opis:: Pomiary pokrytych PEG (poli(glikol etylenowy) nanocząstek magnetytu o średnicy rdzenia 10 nm wykonano metodą spektroskopii elektronowego rezonansu paramagnetycznego w zakresie temperatur 135–235 K. Badano nanocząstki znajdujące się w roztworze wodnym oraz w pełnej krwi ludzkiej. Przed dodaniem nanocząstek do krwi, zidentyfikowano naturalnie występujące w niej centra paramagnetyczne. Widmo EPR nanocząstek magnetytu miało postać szerokiej linii, której parametry silnie zależały od temperatury. W przypadku obu ośrodków zaobserwowano wzrost wartości współczynnika g wraz z obniżaniem temperatury środowiska. W przypadku nanocząstek w wodzie, schładzanie próbki do coraz niższych temperatur powodowało poszerzenie linii EPR, natomiast dla nanocząstek we krwi efekt ten był obserwowalny jedynie w przedziale temperatur od 190 do 235 K. Wartości parametrów linii EPR różniły się dla nanocząstek znajdujących się w dwóch ośrodkach (krwi i wodzie), co może wskazywać na wpływ środowiska na wzajemne interakcje nanocząstek.
The measurements of PEG-coated magnetite nanoparticles (PEG – polyethyleneglicol) with a core diameter of 10 nm, were done by means of Electron Paramagnetic Resonance spectroscopy at the temperature range 135–235 K. Nanoparticles were examined in aqueous solution and in whole human blood. Paramagnetic centers naturally occurring in the blood were identified prior to the addition of nanoparticles. The EPR spectrum of magnetite nanoparticles has a form of a broad line, which parameters strongly depend on temperature. The increase in g-factor value was observed in case of both media, when the measurement temperature was being decreased. Gradual cooling of the sample of nanoparticles in water resulted in the broadening of the EPR line, whereas for nanoparticles in the blood this effect was only observed in the temperature range 190 to 235 K. The values of EPR line parameters were different for nanoparticles in two mentioned media (water and blood), which may indicate the influence of the environment on the interactions between nanoparticles.
Źródło:: Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna; 2015, 21, 1; 9-15
1234-5563
Pojawia się w:: Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Major, minor, and trace elements in whole blood of patients with different leukemia patterns
Autorzy:: Khuder, A.
Bakir, M. A.
Solaiman, A.
Issa, H.
Habil, K.
Mohammad, A.
Powiązania:: https://bibliotekanauki.pl/articles/146612.pdf
Data publikacji:: 2012
Wydawca:: Instytut Chemii i Techniki Jądrowej
Tematy:: leukemia
principal component analysis
Student's t test
whole blood
X-ray fluorescence
Opis:: The elemental sensitivity method for X-ray fluorescence analysis was applied to determine S, Cl, K, Ca, Fe, Cu, Zn, Br, and Rb in the whole blood of leukemia patients and healthy volunteers. Leukemia samples were classified according to type, growth, and age of participants. Student’s t-test results showed that, the mean concentration of the studied elements was significantly lower in leukemia patients than that in controls. Strong mutual correlations (r greather than 0.50) in the whole blood of leukemia patients were observed between S-Ca, K-Fe, K-Ca, Fe-Zn, K-Zn, K-Rb, Fe-Rb, Zn-Rb, S-Cl, S-K, Ca-Fe, Cl-Ca, and Ca-Rb; whereas, S-K, S-Ca, S-Cl, Cl-K, Cl-Ca, Fe-Zn, Zn-Rb, Fe-Rb, K-Fe, and Zn-Br exhibited strong relationships (r greather than 0.50) in the whole blood of controls, all were significant at p less than 0.05. Significant differences between grouping of studied elements in the control group and all classified leukemia groups, except younger age-group, were obtained using principal component analysis. The study indicated appreciably different patterns of element distribution and mutual relationships in the whole blood of leukemia patients in comparison with controls.
Źródło:: Nukleonika; 2012, 57, 3; 389-399
0029-5922
1508-5791
Pojawia się w:: Nukleonika
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: DNA isolation method from human blood with MasterPure DNA Purification Kit™ – review article
Autorzy:: Florczak, Sylwia
Kempka, Katarzyna
Hołderna, Karolina
Stanek, Emilia
Baszyński, Jędrzej
Kamiński, Piotr
Bogdzińska, Maria
Powiązania:: https://bibliotekanauki.pl/articles/1179641.pdf
Data publikacji:: 2017
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: DNA
human
isolation
optimization
review
whole blood
Opis:: The main element of genomic molecular analysis is isolation of DNA from biological material. DNA isolation is an important process of PCR methods. Efficency of PCR processes depends of purness and concentration of DNA. Many methods allows to izolate good quality DNA. There are many Purification Kits and methods of extraction genomic material. We show available techniques and methods of increasing efficiency of the process in our studies. Previous data shows that operating with kits needs optimization, whilst protocols do not have all information about isolation process. We concentrate on Epicentre MasterPure DNA Purification Kit, which is cheap, fast and allows to purification nucleic acids from various materials. We show detailed advantages and disadvantages of this set. Our main aim is to develop protocol to get optimal concentration (50-100 ng/μL) and pureness (A 260/280; 1.6-1.9) of DNA. We also should optimize the process of DNA isolation to obtain efficiency of about 100%. Optimized methodology of DNA isolation, with using MasterPure DNA Purification Kit allows to get good quality material in laboratory without specialistic equipment. This survey contains our assumptions of ways how we can influent on purification process according to previous data.
Źródło:: World Scientific News; 2017, 72; 69-76
2392-2192
Pojawia się w:: World Scientific News
Dostawca treści:: Biblioteka Nauki

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