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Wyświetlanie 1-15 z 91
Tytuł:
W zakresie edukacji, życia i całości
Autorzy:
Dancák, Pavol
Powiązania:
https://bibliotekanauki.pl/articles/2141622.pdf
Data publikacji:
2010
Wydawca:
Instytut Studiów Międzynarodowych i Edukacji Humanum
Tematy:
Education
Man
Whole
Opis:
Nowadays the problem of education is still loaded with modern spiritualizm and its atmosphere in education, and didactics in education. The problem of education is not only a challenge to an abstract academic reflection, but it is actual mission of life in the context of the civilization development, which end up in the crisis. There is call for an authentic life-long education as a human effort.
Źródło:
Humanum. Międzynarodowe Studia Społeczno-Humanistyczne; 2010, 1(4); 21-32
1898-8431
Pojawia się w:
Humanum. Międzynarodowe Studia Społeczno-Humanistyczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Spektrometria całego ciała w Polsce – nauka, medycyna, dozymetria
Whole body spectrometry in Poland – science, medicine, dosimetry
Autorzy:
Ośko, Jakub
Kierepko, Renata
Powiązania:
https://bibliotekanauki.pl/articles/985850.pdf
Data publikacji:
2020
Wydawca:
Indygo Zahir Media
Tematy:
spektrometria całego ciała
LPCC NCBJ
SCC IFJ PAN
badania in vivo
whole body spectrometry
NCBJ whole body counter
IFJ PAN whole body spectrometer
in vivo measurements
Opis:
Spektrometria całego ciała realizowana jest na terenie Polski w oparciu o dwa urządzenia zlokalizowane w Narodowym Centrum Badań Jądrowych w Świerku oraz w Instytucie Fizyki Jądrowej Polskiej Akademii Nauk w Krakowie. Ta technika pomiarowa, stosowana w powiązaniu z odpowiednio przygotowanymi kalibracjami wydajnościowymi (opracowanymi w oparciu o wykorzystanie fantomów antropometrycznych i/lub modeli numerycznych), umożliwia jakościową i ilościową analizę izotopów gamma – promieniotwórczych zdeponowanych w ciele człowieka. Spektrometry całego ciała w Polsce wykorzystywane są do rutynowego monitoringu osób narażonych zawodowo, monitoringu pracowników i pacjentów medycyny nuklearnej oraz ich rodzin, a także badań osób potencjalnie skażonych w wyniku incydentów/zdarzeń jądrowych.
Whole body spectrometry is carried out in Poland based on two devices located in the National Center for Nuclear Research in Świerk and the Institute of Nuclear Physics Polish Academy of Sciences in Krakow. This measuring technique, used together with properly prepared performance calibrations (developed based on anthropometric phantoms and / or numerical models), enables qualitative and quantitative analysis of gamma- radioactive isotopes deposited in the human body. Whole body spectrometers in Poland are used for routine monitoring of occupationally exposed personnel, monitoring of employees and patients in the field of nuclear medicine and their families, as well as research on persons potentially contaminated as a result of nuclear incidents / events.
Źródło:
Inżynier i Fizyk Medyczny; 2020, 9, 1; 27-30
2300-1410
Pojawia się w:
Inżynier i Fizyk Medyczny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Latest developments in international standardization of whole-body and hand-arm vibration
Autorzy:
Liedtke, M.
Rissler, J.
Kaulbars, U.
Powiązania:
https://bibliotekanauki.pl/articles/177663.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
standards
whole-body vibration
hand-arm vibration
Opis:
Latest developments in international standardization of whole-body and hand-arm vibration are pre- sented. In addition, two German projects are presented that might have impact on international work programs in the next years.
Źródło:
Archives of Acoustics; 2013, 38, 4; 555-558
0137-5075
Pojawia się w:
Archives of Acoustics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Formelhaft oder nicht? Die wichtigsten Merkmale der formelhaften Sequenzen
Formulaic or not? The most important characteristics of formulaic sequences
Autorzy:
Czarnecka, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/917007.pdf
Data publikacji:
2018-11-05
Wydawca:
Uniwersytet im. Adama Mickiewicza w Poznaniu
Tematy:
formulaic sequences
formulaicity
language economics
whole memorization
Opis:
Formulaic sequences constitute a large protection of any discourse and, furthermore, they exist in so many forms that it is difficult to develop an adequate definition of this phenomenon. The term formulaic sequences encompasses verious types of world strings which appear to be stored and retrieved as holistic units from the memory. This article outlines some improtant aspects of formulaic sequences; it also shows the variety of definitions which appear in the research literature, and tries to find some specific criteria by means of which formulaic sequences could be identified in a discourse or a text.
Źródło:
Glottodidactica. An International Journal of Applied Linguistics; 2010, 36, 1; 33-43
0072-4769
Pojawia się w:
Glottodidactica. An International Journal of Applied Linguistics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Digit Cards – Creative Activity in Whole Tens
Karty cyfrowe – twórcza aktywność w całych dziesiątkach
Autorzy:
Bassan-Cincinatus, Ronit
Patkin, Dorit
Powiązania:
https://bibliotekanauki.pl/articles/1811065.pdf
Data publikacji:
2019
Wydawca:
Katolicki Uniwersytet Lubelski Jana Pawła II. Towarzystwo Naukowe KUL
Tematy:
całe dziesiątki
całe setki
dodatek
najmniejsza suma
cyfry
whole tens
whole hundreds
addition
smallest sum
digit cards
Opis:
Niektórzy ludzie rozwiązują to samo zadanie na różne „indywidualne” sposoby. Działanie „Aktywność twórcza w całych dziesiątkach” angażuje się w ukierunkowane zadanie eksploracji dodania dwóch liczb, których suma wynosi w całości dziesiątki. Obejmuje: tabelę mnożenia, tabelę setek i tabelę tysięcy. Systematyczne zapisywanie ćwiczeń, liczb, których suma jest w całych dziesiątkach, a rozwiązanie na różne sposoby. Zadania przeznaczone dla 1-szych równiarków i oparte na zasadzie używania „cyfrowych kart”, na których oznaczono cyfry 0 – 9, koncentrują się na dodanych liczbach za pomocą cyfrowych kart, tworzą dwie liczby tak, że ich suma jest w całych dziesiątkach. Wkład: wykonanie „skutecznych” ustnych obliczeń, oszacowań i przybliżeń, uzyskanie poczucia rzędu wielkości i miejsca w systemie liczb, badanie różnych metod rozwiązania, dekomponowanie liczby na komponenty i łączenie liczb, wyciąganie wniosków i zwiększanie wglądów matematycznych.
Some people solve the same assignment in different ‘individual’ ways. The activity “Creative activity in whole tens” engages in a directed assignment of exploring the addition of two numbers whose sum is in whole tens. It includes: a table of multiplication, a table of hundreds and a table of thousands. A systematic recording of exercises, figures whose sum is in whole tens, and solution in different ways. The assignments were designed for 1st to 6th graders and based on the principle of using “digit cards” labelled from 0 – 9 and focused on added numbers using digit cards, form two numbers so that their sum is in whole tens. The contribution is manifold: performing “effective” oral calculations, estimations and approximations; promoting awareness of the order, sequence and place of numbers; examining a variety of the solution methods; decomposing a number into components and combining figures; drawing conclusions and enhancing mathematical insights.
Źródło:
Roczniki Pedagogiczne; 2019, 11(47), 2; 119-131
2080-850X
Pojawia się w:
Roczniki Pedagogiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Przede wszystkim dla dorosłych.... Psychoanalityczna (jungowska) wykładnia baśni Hansa Christiana Andersena
First of All for Adults… Jungian Psychoanalytic Interpretation of H.Ch. Andersen’s Fairytale
Autorzy:
Kerlin, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/545440.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Gdański. Wydział Filologiczny
Tematy:
Whole
psychoanalysis
Big Mother
Shadow
personality
unconsciousness
Opis:
Fairytale is an ancient literary genre which shows the important issues of life process. It is also an expression of man’s struggle between spiritual and material parts of life, which brings the image of the eternal conflicts, desires and aspirations of people. Fairytale seems to speak to a human language derived from ancient eras, and is the result of cultural heritage of mankind stored in human psyche. Heroes of fairytales are most often mortal men, experiencing adventure, rolling buoys, saving others. It’s true, using the language of myth, that heroes despite their imperfections gain power, treasure, or extraordinary powers. The metaphor of life way as a process in which suffering has an equal status as happiness, well-being is intertwined with evil, and fall walks after lifting, shows the way to the Whole man. Carl Gustav Jung, who was the creator of the concept of collective unconscious, believed that it contains elements specific to the entire humanity, independent of historical processes, either ethnic or cultural. He singled out the archetypes or primitive patterns of beliefs, ideas, patterns of living, experience, common to all men. Fairytale style reflects the complexities specific to each psyche. Integration of internal dark forces, birth of individuality, an internal process of growth, overcoming various conflicts and obstacles in the way of individuation are spiritual experiences which every man undergoes. Fairytale brings the opportunity to discover unconscious resources, which equip the fate of every human being and that s/he which consciously uses to build its standalone “I.” We are accustomed to see in fairytale a genre suitable only for children, which in addition to shaping imagination can overcome fears, integrate ignorance tame in terms of psychological or existential everyday experiences to build relationships with others. All of these components are elements of significant awareness of the potential in the development of small humans, but they are also important in achieving fully human adulthood. An adult is not different much from a child in terms of awareness. S/he is also familiar with the difficulties in coping with life, escapes from everyday life, playing down the sphere of the Shadow or the use of defensive mechanisms to avoid responsibility. But what is considered a child in the way of shaping identity in the life of an adult can have important consequences in forming of self-destruction or destruction. Therefore, it becomes important to understand the motivations of human activities, especially with regard to the integration of opposites by the construction of personality, and of how this path should be carried forward if it would lead to performance. It is important to a modern man to know how to play an important role in psychic sphere of his/her life and discover secrets of the unconscious. Then it is possible to find out that a diagnosis or “treatment” of suffering will no longer need any therapist. Fairytales of Hans Christian Andersen are among exceptional pieces. Gifted with extraordinary sensitivity, the author constructed the story of philosophy, which is characterized by great wisdom of life. You can tell that the writer with a thorough insight studied the problem of man’s place in the world, eyeing his/her dilemmas and being there. His observations were gathered in large collections, because this genre was probably the closest to him when it comes to transfer culturally established standards of ethical behaviour.
Źródło:
Jednak Książki. Gdańskie Czasopismo Humanistyczne; 2017, 7; 45-66
2353-4699
Pojawia się w:
Jednak Książki. Gdańskie Czasopismo Humanistyczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Short communication: directing cheerleader’s gaze influences motor behaviour in somersaults
Autorzy:
Heinen, Thomas
Vollmer, Hannah
Powiązania:
https://bibliotekanauki.pl/articles/1054864.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
Tematy:
complex skill
visual perception
whole-body rotation
Opis:
Eye, head, and body movements are thought to be functionally coupled in the performance of complex skills, such as somersaults with and without twists (Davlin et al. 2001; von Laßberg et al. 2014). Directing the gaze to specific locations in the environment during the takeoff phase in somersaults could influence takeoff kinematics and, as a consequence, the subsequent flight phase. The goal of this study was to investigate the relationship between gaze direction during takeoff and the corresponding movement kinematics when performing backward somersaults in cheerleading. N = 11 cheerleaders performed standing backward somersaults in three experimental conditions (straight gaze direction, elevated gaze direction, depressed gaze direction). Results revealed that cheerleaders exhibited a larger hip angle during takeoff and longer flight duration of the somersaults when their gaze was elevated during the takeoff phase, thereby supporting the notion that a functional coupling seems to operate between gaze behaviour and motor behaviour in cheerleading. In daily practice, cheerleaders could try to focus their gaze on a point on the wall in front of them during the takeoff phase in somersaults in order to facilitate the development of a functional coupling between gaze behavior and motor behavior.
Źródło:
Central European Journal of Sport Sciences and Medicine; 2014, 7, 3; 23-28
2300-9705
2353-2807
Pojawia się w:
Central European Journal of Sport Sciences and Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DNA isolation method from human blood with MasterPure DNA Purification Kit™ – review article
Autorzy:
Florczak, Sylwia
Kempka, Katarzyna
Hołderna, Karolina
Stanek, Emilia
Baszyński, Jędrzej
Kamiński, Piotr
Bogdzińska, Maria
Powiązania:
https://bibliotekanauki.pl/articles/1179641.pdf
Data publikacji:
2017
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
DNA
human
isolation
optimization
review
whole blood
Opis:
The main element of genomic molecular analysis is isolation of DNA from biological material. DNA isolation is an important process of PCR methods. Efficency of PCR processes depends of purness and concentration of DNA. Many methods allows to izolate good quality DNA. There are many Purification Kits and methods of extraction genomic material. We show available techniques and methods of increasing efficiency of the process in our studies. Previous data shows that operating with kits needs optimization, whilst protocols do not have all information about isolation process. We concentrate on Epicentre MasterPure DNA Purification Kit, which is cheap, fast and allows to purification nucleic acids from various materials. We show detailed advantages and disadvantages of this set. Our main aim is to develop protocol to get optimal concentration (50-100 ng/μL) and pureness (A 260/280; 1.6-1.9) of DNA. We also should optimize the process of DNA isolation to obtain efficiency of about 100%. Optimized methodology of DNA isolation, with using MasterPure DNA Purification Kit allows to get good quality material in laboratory without specialistic equipment. This survey contains our assumptions of ways how we can influent on purification process according to previous data.
Źródło:
World Scientific News; 2017, 72; 69-76
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Musculoskeletal symptoms and exposure to whole-body vibration among open-pit mine workers in the Arctic
Autorzy:
Burström, Lage
Aminoff, Anna
Björ, Bodil
Mänttäri, Satu
Nilsson, Tohr
Pettersson, Hans
Rintamäki, Hannu
Rödin, Ingemar
Shilov, Victor
Talykova, Ljudmila
Vaktskjold, Arild
Wahlström, Jens
Powiązania:
https://bibliotekanauki.pl/articles/2161904.pdf
Data publikacji:
2017-06-19
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
questionnaire
mining
vibration
MSD
whole-body
Barents
Opis:
Objectives This cross-sectional questionnaire study was carried out at 4 open-pit mines in Finland, Norway, Russia and Sweden as part of the MineHealth project. The aim has been to compare the prevalence of musculoskeletal symptoms between drivers of mining vehicles and non-drivers. Material and Methods The mine workers were asked whether they had suffered from any musculoskeletal symptoms during the previous 12 months in specified body regions, and to grade the severity of these symptoms during the past month. They were also asked about their daily driving of mining vehicles. Results The questionnaire was completed by 1323 workers (757 vehicle drivers) and the reported prevalence and severity of symptoms were highest for the lower back, followed by pain in the neck, shoulder and upper back. Drivers in the Nordic mines reported fewer symptoms than non-drivers, while for Russian mine workers the results were the opposite of that. The daily driving of mining vehicles had no significant association with the risk of symptoms. Female drivers indicated a higher prevalence of symptoms as compared to male drivers. Conclusions The study provided only weak support for the hypothesis that drivers of vehicles reported a higher prevalence of musculoskeletal symptoms than non-vehicle drivers. There were marked differences in the prevalence of symptoms among workers in various enterprises, even though the nature of the job tasks was similar. Int J Occup Med Environ Health 2017;30(4):553–564
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2017, 30, 4; 553-564
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ rodzaju podłoża oraz zmiany środka ciężkości użytkownika na komfort jazdy wózkiem inwalidzkim
The influence of the road pavement type and user’s gravity center on the wheelchair driving comfort
Autorzy:
Chwalik, G.
Kromka-Szydek, M.
Dziechciowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/99344.pdf
Data publikacji:
2017
Wydawca:
Politechnika Śląska. Katedra Biomechatroniki
Tematy:
wózek inwalidzki
drgania ogólne
wheelchair
whole-body vibration
Opis:
Celem pracy była ocena komfortu jazdy użytkownika uniwersalnego wózka inwalidzkiego. Dokonano oceny ryzyka związanego z krótkotrwałym działaniem drgań mechanicznych na osobę znajdującą się na wózku w zależności od rodzaju nawierzchni, po której się poruszała (badanie przeprowadzono na 13 różnych nawierzchniach). Przeanalizowano również wpływ zmiany środka ciężkości osoby na wózku, jaki ma miejsce przy zmianie postawy w trakcie jazdy czynnej i biernej.
This study examined the effect of changes the centre of gravity and road surface types on using of a wheelchair. Users comfort, while using wheelchair, was specified during vibration measurement tests. The vibration was measured in each of three axes. For the purpose of this thesis the universal folding wheelchair Budget 9.050 of Meyra company was used. The measurements were performed on 13 different surfaces. Each test was executed twice for two different positions of wheelchair’s user. In the first test, user was sitting on a wheelchair and was pushed by the operator. In the second attempt, user was driving wheelchair by himself.
Źródło:
Aktualne Problemy Biomechaniki; 2017, 12; 15-20
1898-763X
Pojawia się w:
Aktualne Problemy Biomechaniki
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dosimetry of 131 I for Occupational Radiation Workers by Whole Body Counting System
Autorzy:
Rahman, M. S.
Haydar, M. A.
Patwary, M. K. A.
Shome, S. M.
Paul, S.
Paul, D.
Meaze, A. K. M. M. H.
Powiązania:
https://bibliotekanauki.pl/articles/411910.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Internal Dosimetry
Whole Body Counter
IMBA
131I
Opis:
Internal radiation dosimetry of occupational radiation workers due to inhalation of 131 I during maintenance at iodine 131 I hot cell of Radioisotope Production Division (RIPD) of Bangladesh Atomic Energy Commission have been performed with Whole Body Counter (WBC) containing two large size NaI detector. The contaminated radioactivity of the radiation workers by 131 I was immediately conducted using a WBC (CANBERRA, FASTSCAN model 2250) and counting was repeated again a few days later. The performance of whole body counter was checked by CANBERRA transfer phantom (model 2257) with a mixed source (20 ml vial). The intake was calculated by Apex-Invivo counting software and then corresponding dose in thyroid were estimated by using Integrated Modules for Bioassay Analysis (IMBA) Professional Plus code modules. The body activity due to intake of 131 I and the total effective and equivalent dose in thyroid were also estimated and discussed.
Źródło:
International Letters of Chemistry, Physics and Astronomy; 2014, 20, 1; 21-27
2299-3843
Pojawia się w:
International Letters of Chemistry, Physics and Astronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Współczesna krioterapia ogólnoustrojowa w odnowie biologicznej
Contemporary whole-body cryotherapy in wellness
Autorzy:
Stanek, Agata
Sieroń, Aleksander
Powiązania:
https://bibliotekanauki.pl/articles/1038824.pdf
Data publikacji:
2012
Wydawca:
Śląski Uniwersytet Medyczny w Katowicach
Tematy:
krioterapia ogólnoustrojowa
kriostymulacja ogólnoustrojowa
kriokomora
odnowa biologiczna
osoby zdrowe
whole-body cryotherapy
whole-body cryostimulation
cryochamber
wellness
healthy individuals
Opis:
One of the methods of physical medicine which is used more and more frequently in wellness is whole-body cryotherapy (whole-body cryostimulation). It consists in stimulating the whole body surface by applying cryogenic temperatures (below -100°C) for a short time of 1–3 minutes. The effect of cryogenic temperatures on the whole body evokes in the human organism several, beneficial physiological reactions such as analgesic, neuro-muscular, antiodematous and anti-inflammatory effects as well as circulatory, hormonal and immunological reactions. Whole-body cryostimulation procedures also have a beneficial influence on the human psyche through the action of relaxing and decreasing the level of anxiety.
Jedną z metod medycyny fizykalnej, coraz częściej znajdującą zastosowanie w odnowie biologicznej, jest krioterapia (kriostymulacja) ogólnoustrojowa. Polega ona krótkotrwałym, bodźcowym stosowaniu temperatur kriogenicznych (poniżej -100°C), w krótkim czasie 1–3 minut na całą powierzchnię ciała pacjenta. Działanie temperatur kriogenicznych stosowanych ogólnoustrojowo wywołuje w organizmie człowieka wiele korzystnych reakcji fizjologicznych, takich jak: efekt przeciwbólowy, nerwowo- mięśniowy, przeciwobrzękowy, przeciwzapalny oraz krążeniowy, hormonalny i immunologiczny. Zabiegi kriostymulacji ogólnoustrojowej wykazują także korzystny wpływ na psychikę człowieka poprzez działanie relaksujące i obniżenie poziomu lęku.
Źródło:
Annales Academiae Medicae Silesiensis; 2012, 66, 4; 64-70
1734-025X
Pojawia się w:
Annales Academiae Medicae Silesiensis
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Risk exposure to vibration and noise in the use of agricultural track-laying tractors
Autorzy:
Vallone, Mariangela
Bono, Filippa
Quendler, Elisabeth
Febo, Pierluigi
Catania, Pietro
Powiązania:
https://bibliotekanauki.pl/articles/989731.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
noise
safety
track-laying tractor
whole body vibration
Opis:
Human exposure to mechanical vibration may represent a significant risk factor for exposed workers in the agricultural sector. Also, noise in agriculture is one of the risk factors to be taken into account in the evaluation of workers’ health and safety. One of the major sources of discomfort for the workers operating a tractors is the noise to which they are exposed during work. The aim of this study was to evaluate the risk of exposure to whole-body vibration for the operator driving track-laying tractors in vineyard orchard and the noise level. The experimental tests were performed with six different track-laying tractors coupled with the same rototilling machine. The results showed that the vibration values of track-laying tractors coupled to rototilling machine, referred to the 8-hour working day, were always higher than 0.5 m s-2, the daily exposure action value established by Directive 2002/44/EC of the European Parliament. The daily noise exposure levels always exceeded the exposure limit value of 87 dB(A) established by Directive 2003/10/EC of the European Parliament. The ANOVA repeated measures model showed that the factor ‘site’, namely, the soil characteristics, did not influence the vibration level on the X and Y-axes of the tractors measured, regardless of their age. In the Z-axis, the vibration level was enhanced as the soil structure increased. As tractor age increased, the influence of soil characteristics was less important. In term of the age of the tractor and the number of hours worked, it was possible to identify three risk classes, which were up to 3,000 hours worked and offered a low risk; from 3,000 – 6,000 hours worked with a medium risk, and over 6,000 hours with a high risk level.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of the effects of antibiotics on cytotoxicity of EGFP and DsRed2 fluorescent proteins used for stable cell labeling
Autorzy:
Zakrzewska, Karolina
Samluk, Anna
Pluta, Krzysztof
Pijanowska, Dorota
Powiązania:
https://bibliotekanauki.pl/articles/1039220.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
stable fluorescent labeling
whole-cell labeling
fluorescent protein cytotoxicity
Opis:
The use of fluorescent markers has proven to be an attractive tool in biological imaging. However, its usefulness may be confined by the cytotoxicity of the fluorescent proteins. In this article, for the first time, we have examined an influence of the antibiotics present in culture medium on cytotoxicity of the EGFP and DsRed2 markers used for whole-cell labeling. Results showed that doxycycline negatively affected albumin synthesis in DsRed2-expressing hepatoma cells, and that both hepatoma cells and human skin fibroblasts, labeled with this protein, were characterized by the lowered growth rates. Thus, the cytotoxic effect of fluorescent markers depends on both protein used for cell labeling and on growth conditions that may cause cell stress.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 809-813
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on Simultaneous Impact of Hand–Arm and Whole-Body Vibration
Autorzy:
Kowalski, P.
Zając, J.
Powiązania:
https://bibliotekanauki.pl/articles/91046.pdf
Data publikacji:
2012
Wydawca:
Centralny Instytut Ochrony Pracy
Tematy:
vibrotactile perception
hand-arm vibration
whole-body vibration
combined exposure
Opis:
This article presents the results of laboratory tests on the combined effect of whole-body vibration (WBV) and hand–arm vibration (HAV). The reactions of subjects exposed to various combinations of vibration were recorded. The vibrotactile perception threshold (VPT) test identified changes caused by exposure to vibration. Ten male subjects met the criteria of the study. There were 4 series of tests: a reference test and tests after exposure to HAV, WBV, and after simultaneous exposure to HAV and WBV. An analysis of the results (6000 ascending and descending VPTs) showed that the changes in VPTs were greatest after simultaneous exposure to both kinds of vibration. The increase in VPT, for all stimulus frequencies, was then higher than after exposure to HAV or WBV only.
Źródło:
International Journal of Occupational Safety and Ergonomics; 2012, 18, 1; 59-66
1080-3548
Pojawia się w:
International Journal of Occupational Safety and Ergonomics
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 91

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