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Tytuł:
Sytuacja epidemiologiczna włośnicy w Polsce - I półrocze 2022 r.
Epidemiology of trichinellosis in Poland - the first half of 2022
Autorzy:
Bełcik, Aneta
Korpysa-Dzirba, Weronika
Bilska-Zając, Ewa
Różycki, Mirosław
Gontarczyk, Aneta
Kochanowski, Maciej
Samorek-Pieróg, Małgorzata
Karamon, Jacek
Cencek, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/22443123.pdf
Data publikacji:
2022
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
dziki
sytuacja epidemiologiczna
rok 2022
zwierzęta dziko żyjące
zwierzęta łowne
choroby zwierząt
choroby pasożytnicze
włośnica
występowanie
trichinellosis
wild boars
hunting animals
Polska
Opis:
The aim of this study was to present the current situation of trichinellosis, basing on the results of examination of the tissues of animals hunted in Poland. Investigation was carried out from 1st January to 30th June 2022 in the Department of Parasitology and Parasitic Diseases of National Veterinary Research Institute in Poland. Samples were collected out of 103 wild boars (Sus scrofa) and one wolf (Canis lupus), originating from 14 voivodeships. The study of wild boars muscle tissues revealed 89 positive samples, including 60 T. spiralis, 21 T. britovi, two T. pseudospiralis, and five mixed invasions with T. spiralis and T. britovi. In one case no amplification products were obtained. T. britovi was also found in the sample of wolf muscle tissue. This study emphasize the fact that trichinellosis in hunting animals is still a zoonotic threat, and should be closely monitored.
Źródło:
Życie Weterynaryjne; 2022, 97, 09; 607-610
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena wyników badania sanitarno-weterynaryjnego koni rzeźnych w Polsce w 2016 r.
Evaluation of the sanitary and veterinary inspection of slaughter horses in Poland in 2016
Autorzy:
Lis, H.
Gorski, K.
Powiązania:
https://bibliotekanauki.pl/articles/859675.pdf
Data publikacji:
2018
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
konie
konie rzezne
badania sanitarno-weterynaryjne
badania poubojowe
zmiany chorobowe
posocznica
ropnica
tezec
nowotwory
nadmierne wychudzenie
wodnica
zoltaczka
rozklad gnilny
niedostateczne wykrwawienie
pasozyty zwierzat
wlosnica
wystepowanie
wyniki badan
Polska
rok 2016
Źródło:
Życie Weterynaryjne; 2018, 93, 04
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena wyników badania sanitarno‑weterynaryjnego zwierząt łownych w Polsce w latach 2010 i 2016
Evaluation of veterinary and sanitary investigations of game animals in Poland during 2010–2016
Autorzy:
Lis, H.
Iwanina, M.
Powiązania:
https://bibliotekanauki.pl/articles/860982.pdf
Data publikacji:
2018
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
Polska
zwierzeta lowne
sarny
daniele
jelenie
losie
dziki
muflony
tusze zwierzece
badania sanitarno-weterynaryjne
wyniki badan
zmiany chorobowe
wychudzenie
wodnica
niedostateczne wykrwawienie
rozklad gnilny
posocznica
ropnica
pasozyty zwierzat
wagrzyca
wlosnica
motylica watrobowa
wystepowanie
czestotliwosc wystepowania
rok 2010
rok 2016
Źródło:
Życie Weterynaryjne; 2018, 93, 01
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena wyników badania sanitarno‑weterynaryjnego świń rzeźnych w Polsce w 2016 r.
The evaluation of sanitary and veterinary inspection results of the slaughter pigs in Poland in 2016
Autorzy:
Lis, H.
Gorski, K.
Powiązania:
https://bibliotekanauki.pl/articles/858947.pdf
Data publikacji:
2017
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
trzoda chlewna
zywiec wieprzowy
badania sanitarno-weterynaryjne
badania przedubojowe
badania poubojowe
zmiany chorobowe
gruzlica
rozyca
posocznica
ropnica
salmoneloza
promienica
nowotwory
bialaczka
wychudzenie
wodnica
rozklad gnilny
wagrzyca
wlosnica
wystepowanie
czestotliwosc wystepowania
rok 2016
Polska
Źródło:
Życie Weterynaryjne; 2017, 92, 12
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Choroby odzwierzęce i czynniki zoonotyczne w Europie w 2012 r. - raport Europejskiego Urzędu ds. Bezpieczeństwa Żywności (EFSA)
Zoonoses and zoonotic agents in Europe in 2012 - the European Food Safety Authority (EFSA) report
Autorzy:
Osek, J.
Wieczorek, K.
Powiązania:
https://bibliotekanauki.pl/articles/862375.pdf
Data publikacji:
2014
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
choroby odzwierzece
czynniki chorobotworcze
bruceloza
gruzlica
kampylobakterioza
listerioza
salmoneloza
wscieklizna
wlosnica
wystepowanie
Unia Europejska
Europejski Urzad ds.Bezpieczenstwa Zywnosci
raporty
rok 2012
Źródło:
Życie Weterynaryjne; 2014, 89, 06
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Trichinella pseudospiralis – nowy gatunek włośnia w środowisku leśnym w Polsce. Czy wzrasta zagrożenie włośnicą dla ludzi?
Trichinella pseudospiralis – a new species of Trichinella in sylvatic cycle in Poland. Does the treat of trichinellosis in humans increase?
Autorzy:
Gawor, J.
Powiązania:
https://bibliotekanauki.pl/articles/858895.pdf
Data publikacji:
2013
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
Polska
srodowisko lesne
lisy
dziki
pasozyty zwierzat
Trichinella pseudospiralis
wystepowanie
wlosnica
rozprzestrzenianie sie chorob
Źródło:
Życie Weterynaryjne; 2013, 88, 12
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wplyw albendazolu na cytoplazmatyczna transferaze-S-glutationowa i ekspresje receptorow Toll-podobnych w jelitowej fazie wlosnicy
The influence of albendazole on the cystolic glutatione-S-transferase [GST] and on the expression of Toll-like receptors [TLR2 and TLR4] during the intestinal phase of trichinellosis
Autorzy:
Wojtkowiak-Giera, A
Powiązania:
https://bibliotekanauki.pl/articles/839997.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
choroby pasozytnicze
wlosnica
faza jelitowa
enzymy detoksykacyjne
transferaza S-glutationowa
receptory Toll-podobne
ekspresja receptorow
leki przeciwpasozytnicze
albendazol
Źródło:
Annals of Parasitology; 2010, 56, 2; 181-183
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ albendazolu na cytoplazmatycznę transferazę-S-glutationową i ekspresję receptorów Toll-podobnych w jelitowej fazie włośnicy
The influence of albendazole on the cystolic glutatione-S-transferase [GST] and on the expression of Toll-like receptors [TLR2 and TLR4] during the intestinal phase of trichinellosis
Autorzy:
Wojtkowiak-Giera, A.
Powiązania:
https://bibliotekanauki.pl/articles/2143438.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
choroby pasozytnicze
wlosnica
faza jelitowa
enzymy detoksykacyjne
transferaza S-glutationowa
receptory Toll-podobne
ekspresja receptorow
leki przeciwpasozytnicze
albendazol
Opis:
Research into changes in the activity and kinetics of the reaction catalysed by the important detoxificating enzyme, glutathione-S-transferase (GST), in the intestinal phase of trichinellosis as presented in the present study is a pioneering achievement. In every instance of infection – or if infection is only suspected – the patient receives anthelmintics that are effective against mature intestinal forms, newborn larvae and encysted larvae of Trichinella spiralis. This group of drugs includes benzimidazoles such as albendazole. The primary action of these drugs concerns β-tubuline. The functions of microtubules are damaged by bonding β-tubuline with benzimidazoles, which inhibits the polymerisation of microtubules. Newly considered fields of action of benzimidazoles on the pathological changes occurring in the host during the course of parasitic diseases are the following elements taking part in the immunological defence against a parasitic infection: for example, detoxificating enzymes such as glutathione-Stransferase (GST), and Toll-like receptors (TLRs). Occurrence of cell resistance to drugs is attributed to certain enzymes, (such as GST), which perform the detoxification of these drugs and facilitate their removal from cells through a pump dependent on compounds coupled with glutathione. In the case of the intestinal phase of trichinellosis, GST may remove toxins secreted by both the adult and newborn larvae of T. spiralis. The structure of a dimeric enzyme of the host, such as GST, may be impacted by various factors, for example excretory-secretory products (ES), as well as drugs used. Over the past few years, the interest of researchers in the innate immune response as the first line of defense of vertebrates against of pathogens, was increased. The number of works devoted to the role of Toll-like receptors, which enable the immediate commencement of protective action by the host against various infections without engaging lymphocytes, is on the noticeable. The experimental material consisted of small and large intestine from BALB/c mice infected with T. spiralis strain MSUS/PO/60/ISS3. Mice were inoculated orally with about 400 T. spiralis larvae per mouse. On the 2nd day post-infection, the mice were treated with albendazole, in a dose of 0.5 mg/mouse. The activity and kinetics of the GST were determined spectrophotometrically. An evaluation of the expression of TLRs (TLR2 and TLR4 on the mRNA level) was examined using the RT-PCR technique with the appropriate starter sequences for TLR2 and TLR4. The presence of Toll-like receptors and their expression on the protein level was confirmed using the immunohistochemical method (Santa Cruz ABC Stainig system sc-2023 test) with specific polyclonal antibodies antiTLR2 and anti-TLR4. My results pointed to the participation of GST, as well as Toll-like receptors, in defence processes of the host during the intestinal phase of trichinellosis in mice. The adult and newborn larvae of T. spiralis present in the intestines of infected mice are a source of excretory-secretory (ES) products, which appear to stimulate the activity and change catalytic properties of conformers GST. It is supposed that ES products secreted by the parasite acts on GST as an allosteric activator. Additionally, the presence of Toll-like receptors (TLR2 and TLR4) in the intestinal phase of trichinellosis was investigated. Thus, the infection with T. spiralis leads to strong biochemical reactions with the involvement of GST, and immunological reactions with the involvement of TLR2 and TLR4 receptors. Administration of albendazole, resulted in 2-fold decrease of the total activity of GST in comparison with its activity in infected and untreated animals. Also, drug administration had weakened expression of gene of TLR4 and TLR2 in the large intestine, and expression of gene of TLR2 in the small intestine. My results confirmed destructively action of albendazole on T. spiralis (resulted in 4-fold decrease in the intensity of infection measured in muscles). The destruction by the drug of a adult and newborn larvae of T. spiralis lowers the quantities of their ES products stimulated the activation of the host’s response. This is apparent as weakening the innate immune response, in which take part Toll-like receptors play a role, and in biochemical processes with the participation of the GST enzyme. In summary my results contribute new data on the participation of Toll-like receptors and GST in the defence mechanisms of the host during in the intestinal phase of experimental trichinellosis in mice.
Źródło:
Wiadomości Parazytologiczne; 2010, 56, 2; 181-183
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidemiologia wlosnicy w Polsce dawniej i dzis
Epidemiology of human trichinellosis in Poland - currently and in the past
Autorzy:
Golab, E
Sadkowska-Todys, M.
Powiązania:
https://bibliotekanauki.pl/articles/841256.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
zagrozenia zdrowia
historia
Polska
pasozyty
nicienie
epidemiologia
Warszawa konferencja
wystepowanie
choroby czlowieka
parazytologia
konferencje
Trichinella
Opis:
Since the XIX century human trichinellosis has remained an unsolved problem of public healthcare in Poland. This paper describes the past situation and analyses current changes in the epidemiological pattern of trichinellosis in Poland. Epidemiological data from the last 60 years, point out that the number of human cases as well as the number of deaths caused by trichinellosis has decreased significantly. Up to 90s the main source of Trichinella infection for people was pork. Among other implemented control measures, the introduction of the artificial digestion method in the early 80s to detect trichinellosis in pigs resulted in a shift in the sources of Trichinella infection in humans - pork was replaced with wild boar meat. In the years 1990-1995 the number of outbreaks due to pork consumption was 3.5-times higher than in the years 2000-2005. In the early nineties pork was the source of infection causing about 71% of all outbreaks; in 2000-2005 that number has fallen to only 12%. On the other hand wild boar meat was responsible for 23% of the outbreaks in 1990-1995 and as many as 88% of all outbreaks in the years 2000-2005. Moreover the number of persons infected in the outbreaks significantly decreased. The study of wild animals demonstrated that wild boars in Poland are infected not only with T. spiralis but also with Trichinella britovi. These results and EU recommendations indicate a requirement of determining the Trichinella species which cause infections in outbreaks. In the 3 trichinellosis outbreaks in 2005 the infected meat products were examined with molecular tools. T. spiralis species larvae were the etiological agents of infection in all these outbreaks. The current epidemiological situation of trichinellosis in Poland indicates a need of increasing the awareness of risks related to wild boar meat consumption among the general public. Introducing the artificial digestion method as an obligatory method for wild boar meat examination is also necessary.
Źródło:
Annals of Parasitology; 2006, 52, 3; 181-187
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidemiologia włośnicy w Polsce dawniej i dziś
Epidemiology of human trichinellosis in Poland - currently and in the past
Autorzy:
Gołąb, E.
Sadkowska-Todys, M.
Powiązania:
https://bibliotekanauki.pl/articles/2144340.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
zagrozenia zdrowia
historia
Polska
pasozyty
nicienie
epidemiologia
Warszawa konferencja
wystepowanie
choroby czlowieka
parazytologia
konferencje
Trichinella
Opis:
Since the XIX century human trichinellosis has remained an unsolved problem of public healthcare in Poland. This paper describes the past situation and analyses current changes in the epidemiological pattern of trichinellosis in Poland. Epidemiological data from the last 60 years, point out that the number of human cases as well as the number of deaths caused by trichinellosis has decreased significantly. Up to 90s the main source of Trichinella infection for people was pork. Among other implemented control measures, the introduction of the artificial digestion method in the early 80s to detect trichinellosis in pigs resulted in a shift in the sources of Trichinella infection in humans - pork was replaced with wild boar meat. In the years 1990-1995 the number of outbreaks due to pork consumption was 3.5-times higher than in the years 2000-2005. In the early nineties pork was the source of infection causing about 71% of all outbreaks; in 2000-2005 that number has fallen to only 12%. On the other hand wild boar meat was responsible for 23% of the outbreaks in 1990-1995 and as many as 88% of all outbreaks in the years 2000-2005. Moreover the number of persons infected in the outbreaks significantly decreased. The study of wild animals demonstrated that wild boars in Poland are infected not only with T. spiralis but also with Trichinella britovi. These results and EU recommendations indicate a requirement of determining the Trichinella species which cause infections in outbreaks. In the 3 trichinellosis outbreaks in 2005 the infected meat products were examined with molecular tools. T. spiralis species larvae were the etiological agents of infection in all these outbreaks. The current epidemiological situation of trichinellosis in Poland indicates a need of increasing the awareness of risks related to wild boar meat consumption among the general public. Introducing the artificial digestion method as an obligatory method for wild boar meat examination is also necessary.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 181-187
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena tusz miesa przeznaczonego do konsumpcji w kierunku obecnosci larw wlosni metoda kompresorowa i wytrawiania
Evaluation of the meat carcasses assigned for consumption towards the presence of Trichinella larvae by two methods: trichinoscopy and digestion
Autorzy:
Ramisz, A
Balicka-Ramisz, A.
Powiązania:
https://bibliotekanauki.pl/articles/841524.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
zagrozenia zdrowia
mieso z dzika
ocena jakosci
mieso wieprzowe
pasozyty
larwy
Warszawa konferencja
choroby czlowieka
trychinoskopia
wytrawianie
parazytologia
konferencje
Trichinella
tusze zwierzece
wlosnie
wykrywanie
test ELISA
Opis:
The main purpose of this article was to analyse the effectiveness of two methods: trichinoscopy and digestion method for control of animal trichinellosis. It was established that the using of magnetic stirrer improved the digestion of meat and shortened the digestion to 90-100 minutes. It is worthy of notice that the digestion method is three to four times more sensitive than the traditional trichinoscopy. In the years 1999-2004 Trichinella larvae were found in pork meat 6 times rarely comparing with the years 1990-1994. The introduction of digestion method resulted directly in the reduction (4 times) of the Trichinella infection in humans in the years 2000-2004 comparing with the years 1990-1994. The ELISA test appeared to be also very useful for epidemiological studies on Trichinella infection in animals and humans.
Źródło:
Annals of Parasitology; 2006, 52, 3; 199-204
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena tusz mięsa przeznaczonego do konsumpcji w kierunku obecności larw włośni metodą kompresorową i wytrawiania
Evaluation of the meat carcasses assigned for consumption towards the presence of Trichinella larvae by two methods: trichinoscopy and digestion
Autorzy:
Ramisz, A.
Balicka-Ramisz, A.
Powiązania:
https://bibliotekanauki.pl/articles/2144350.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
zagrozenia zdrowia
mieso z dzika
ocena jakosci
mieso wieprzowe
pasozyty
larwy
Warszawa konferencja
choroby czlowieka
trychinoskopia
wytrawianie
parazytologia
konferencje
Trichinella
tusze zwierzece
wlosnie
wykrywanie
test ELISA
Opis:
The main purpose of this article was to analyse the effectiveness of two methods: trichinoscopy and digestion method for control of animal trichinellosis. It was established that the using of magnetic stirrer improved the digestion of meat and shortened the digestion to 90-100 minutes. It is worthy of notice that the digestion method is three to four times more sensitive than the traditional trichinoscopy. In the years 1999-2004 Trichinella larvae were found in pork meat 6 times rarely comparing with the years 1990-1994. The introduction of digestion method resulted directly in the reduction (4 times) of the Trichinella infection in humans in the years 2000-2004 comparing with the years 1990-1994. The ELISA test appeared to be also very useful for epidemiological studies on Trichinella infection in animals and humans.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 199-204
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Przydatność ELISA do diagnozowania włośnicy u świń i dzików
The usefulness of ELISA for diagnosis of trichinellosis in pigs and wild boars
Autorzy:
Bień, J.
Powiązania:
https://bibliotekanauki.pl/articles/2144342.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
choroby pasozytnicze
diagnostyka weterynaryjna
Polska
walidacja
pasozyty
Warszawa konferencja
trzoda chlewna
dziki
wykorzystanie
parazytologia
konferencje
Trichinella
choroby zwierzat
test ELISA
wykrywanie
Opis:
ELISA can be used to measure produced antibodies or Trichinella spp. antigens in the samples. They are detected with antibodies linked to an enzyme that reacts with a substrate and generate a colour reaction. The optical density (OD) of the reaction is measured spectrophotometrically. ELISA assays can be done in several different procedures called „direct”, "indirect", "sandwich", and "competition" ELISA. Since the 1970s, the studies have been done on improving or replacing direct methods of Trichinella diagnosis with serological methods based on the ELISA. When somatic antigens of L1 T. spiralis were used, the specificity of the ELISA was poor due to a high probability of cross-reactions with other pathogens. During the 1980s the specificity of the ELISA was improved by excretory-secretory (E/S) antigens obtained during Trichinella muscle larvae incubation in vitro. Recently a synthetic glycan antigen has been developed and the increasing of ELISA specificity and sensitivity was noticed. The sensitivity of the ELISA using an E/S antigen ranging from 93.1 to 99.2% but the specificity from 90.6 to 99.4%. The ELISA method is relatively simple to apply, reliable, readily standardized and provides an acceptable balance of sensitivity and specificity. But all modified procedures should be validated. In Poland, the studies on the usefulness of ELISA for antibodies detection against T. spiralis in pigs and wild animals are limited. Own ELISA procedure was prepared in Pathophysiology Lab. in W. Stefański Institute of Parasitology of PAS. ELISA was used to examine IgG level against L1 T. spiralis in pigs and wild boars serum samples. Of 1474 pig samples, only 12 were positive. Of 1880 wild boar samples only 14 were positive. The results of this study are comparable with performance obtained using commercial sets. The results showed the usefulness of ELISA for T. spiralis diagnosis in pigs and wild boars and confirmed the possibility of use the ELISA test for application in the slaughterhouse.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 205-212
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Przydatnosc ELISA do diagnozowania wlosnicy u swin i dzikow
The usefulness of ELISA for diagnosis of trichinellosis in pigs and wild boars
Autorzy:
Bien, J
Powiązania:
https://bibliotekanauki.pl/articles/840219.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
choroby pasozytnicze
diagnostyka weterynaryjna
Polska
walidacja
pasozyty
Warszawa konferencja
trzoda chlewna
dziki
wykorzystanie
parazytologia
konferencje
Trichinella
choroby zwierzat
test ELISA
wykrywanie
Opis:
ELISA can be used to measure produced antibodies or Trichinella spp. antigens in the samples. They are detected with antibodies linked to an enzyme that reacts with a substrate and generate a colour reaction. The optical density (OD) of the reaction is measured spectrophotometrically. ELISA assays can be done in several different procedures called „direct”, "indirect", "sandwich", and "competition" ELISA. Since the 1970s, the studies have been done on improving or replacing direct methods of Trichinella diagnosis with serological methods based on the ELISA. When somatic antigens of L1 T. spiralis were used, the specificity of the ELISA was poor due to a high probability of cross-reactions with other pathogens. During the 1980s the specificity of the ELISA was improved by excretory-secretory (E/S) antigens obtained during Trichinella muscle larvae incubation in vitro. Recently a synthetic glycan antigen has been developed and the increasing of ELISA specificity and sensitivity was noticed. The sensitivity of the ELISA using an E/S antigen ranging from 93.1 to 99.2% but the specificity from 90.6 to 99.4%. The ELISA method is relatively simple to apply, reliable, readily standardized and provides an acceptable balance of sensitivity and specificity. But all modified procedures should be validated. In Poland, the studies on the usefulness of ELISA for antibodies detection against T. spiralis in pigs and wild animals are limited. Own ELISA procedure was prepared in Pathophysiology Lab. in W. Stefański Institute of Parasitology of PAS. ELISA was used to examine IgG level against L1 T. spiralis in pigs and wild boars serum samples. Of 1474 pig samples, only 12 were positive. Of 1880 wild boar samples only 14 were positive. The results of this study are comparable with performance obtained using commercial sets. The results showed the usefulness of ELISA for T. spiralis diagnosis in pigs and wild boars and confirmed the possibility of use the ELISA test for application in the slaughterhouse.
Źródło:
Annals of Parasitology; 2006, 52, 3; 205-212
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wybrane aspekty patologii klinicznej włośnicy
A selected aspects of clinical pathology of human trichinellosis
Autorzy:
Kocięcka, W.
Powiązania:
https://bibliotekanauki.pl/articles/2144341.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
czynniki chorobotworcze
choroby pasozytnicze
patologia kliniczna
choroby czlowieka
parazytologia
Trichinella
konferencje
pasozyty
Warszawa konferencja
Opis:
In this review the pathomechanisms of human trichinellosis with particular relation to intestinal and muscular invasion are presented. The numerous factors initiated by antigen released by Trichinella, which play a role in the development of pathological process (including immunological, pathomorphological, metabolic and bioelectric disturbances) and short clinical characteristics of the disease are also presented. Pathology of late period of trichninellosis sequelae are discussed.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 189-198
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 64

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