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    Wyświetlanie 1-12 z 12
    Tytuł:
    Detection and differentiation of Newcastle disease virus and influenza virus by using duplex real-time PCR
    Autorzy:
    Nidzworski, Dawid
    Wasilewska, Edyta
    Smietanka, Krzysztof
    Szewczyk, Bogusław
    Minta, Zenon
    Powiązania:
    https://bibliotekanauki.pl/articles/1039554.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Newcastle disease virus
    influenza virus
    detection
    differentiation
    real-time PCR
    duplex
    Opis:
    Newcastle disease virus (NDV), member of the Paramyxoviridae family and avian influenza virus (AIV), member of the Orthomyxoviridae family, are two main avian pathogens causing serious economic problems in poultry health. Both are enveloped, single-stranded, negative-sense RNA viruses and cause similar symptoms, ranging from sub-clinical infections to severe diseases, including decrease in egg production, acute respiratory syndrome, and high mortality. Similar symptoms hinder the differentiation of infection with the two viruses by standard veterinary procedures like clinical examination or necropsy. To overcome this problem, we have developed a new duplex real-time PCR assay for the detection and differentiation of these two viruses. Eighteen NDV strains, fourteen AIV strains, and twelve other (negative control) strains viruses were isolated from allantoic fluids of specific pathogen-free (SPF), embryonated eggs. Four-weeks-old SPF chickens were co-infected with both viruses (NDV - LaSota and AIV - H7N1). Swabs from cloaca and trachea were collected and examined. The results obtained in this study show that by using duplex real-time PCR, it was possible to detect and distinguish both viruses within less than three hours and with high sensitivity, even in case a bird was co-infected. Additionally, the results show the applicability of the real-time PCR assay in laboratory practice for the identification and differentiation of Newcastle disease and influenza A viruses in birds.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 3; 475-480
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of TaqMan-based real-time PCR assay based on the E1 genefor the quantitative detection of the Getah virus
    Autorzy:
    Lin, A.
    Hu, X.
    Cui, S.
    Yang, T.
    Zhang, Z.
    Li, P.
    Guo, M.
    Lu, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647453.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    Getah virus
    real-time PCR
    TaqMan
    detection
    Opis:
    To develop a sensitive, specific, and rapid approach for the detection Getah virus (GETV), a set of primers targeting the conserved region of the E1 gene was created. The TaqMan-based real-time PCR method for GETV detection was developed by optimizing the reaction conditions. The method demonstrated excellent specificity, and amplification did not occur with the causative agents of all prevalent swine viral infections (CSFV, PRRSV, PRV, PEDV, PTV, and JEV), except GETV. Additionally, upon assessing the sensitivity of the method, the minimum detection limit for GETV was found to be 5.94 copies/μL, which is 10 times higher than that of the traditional PCR approach. Further, the intra- and inter-assay variation coefficients were less than 1%, demonstrating good repeatability. Moreover, GETV was found in 10 of the 20 field serum samples using real-time PCR but only in three of the samples using traditional PCR. Consequently, the first GETV TaqMan-based real-time PCR approach based on the E1 gene was developed for GETV pathogenic diagnoses, and this exhibited high specificity, sensitivity, and repeatability. This assay is practical for the pathogenic diagnosis and epidemiology of GETV.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 1; 21-28
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Electrochemical biosensors for detection of avian influenza virus - current status and future trends
    Autorzy:
    Grabowska, Iwona
    Malecka, Kamila
    Jarocka, Urszula
    Radecki, Jerzy
    Radecka, Hanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039249.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    electrochemical biosensors
    genosensors
    immunosensors
    electrochemical detection
    avian influenza virus
    Opis:
    Electrochemical biosensors have emerged as reliable analytical devices suitable for pathogen detection. Low cost, small sample requirement and possibility of miniaturization justifies their increasing development. Thus, we report in this review on the state of the art of avian influenza virus detection with genosensors and immunosensors working by an electrochemical mode. Their working principles focusing on the physical properties of the transducer, the immobilization chemistry, as well as new trends including incorporation of nanoparticles will be presented. Then, we critically review the detection of avian influenza virus in the complex matrices that use electrochemical biosensors and compare them with traditionally applied methods such as ELISA or Western blot.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 471-478
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of the influenza virus yesterday and now
    Autorzy:
    Woźniak-Kosek, Agnieszka
    Kempińska-Mirosławska, Bogumiła
    Hoser, Grażyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039248.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    influenza
    virus
    detection method
    diagnostics
    PCR
    viral respiratory tract infection
    Opis:
    Demographic changes and the development of transportation contribute to the rapid spread of influenza. Before an idea of a 'person to person' spread appeared, divergent theories were developed to explain influenza epidemics in the past. Intensified virological and serological tests became possible after isolation of the human influenza virus in 1933. The first influenza virus detection methods were based on its isolation in egg embryos or cell lines and on demonstration of the presence of the viral antigens. Molecular biology techniques associated with amplification of RNA improved the quality of tests as well as sensitivity of influenza virus detection in clinical samples. It became possible to detect mixed infections caused by influenza types A and B and to identify the strain of the virus. Development of reliable diagnostic methods enabled fast diagnosis of influenza which is important for choosing an appropriate medical treatment.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 465-470
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of barley- and wheat-specific forms of Wheat dwarf virus in their vector Psammotettix alienus by duplex PCR assay
    Autorzy:
    Trzmiel, K.
    Klejdysz, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/66781.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    wheat dwarf virus
    virus
    cereal crop
    detection
    barley
    Psammotettix alienus
    Hemiptera
    Auchenorrhyncha
    Cicadellidae
    leafhopper
    duplex polymerase chain reaction
    Opis:
    Wheat dwarf virus (WDV) has been one of the most common viruses on cereal crops in Poland in the last years. This single stranded DNA virus is transmitted by the leafhopper spec, Psammotettix alienus (Dahlb.) in a persistent manner. It induces yellowing and streaking of leaves, dwarfing or even death of infected plants. The presence of barley- and wheat-specific forms of WDV (WDV-B and WDV-W) and their vector were previously reported in the country, however the literature data did not include any information on the infectivity of the vector in Poland. A duplex polymerase chain reaction (PCR) procedure was developed and optimized for simultaneous detection and differentiation of both forms in the vector. Two sets of primers amplify 734 bp and 483 bp specific fragments for WDV-W and WDV-B, respectively. The results were verified by a sequencing method. The studies were carried out on insect samples collected in autumn from four different locations in Greater Poland. The results confirmed the presence of WDV-W in the tested samples. They also suggested the concomitant of both forms of the virus in the vector. Additional studies to determine virus-vector relationships should be undertaken.
    Źródło:
    Journal of Plant Protection Research; 2018, 58, 1
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serological and molecular detection of bean leaf roll and chickpea chlorotic stunt luteoviruses in chickpea from Iran
    Autorzy:
    Hajiyusef, T.
    Shahraeen, N.
    Maleki, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/66046.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    bean leafroll virus
    serological detection
    molecular detection
    chick-pea chlorotic stunt luteovirus
    chick-pea
    Cicer arietinum
    phylogenesis
    serology
    Iran
    Opis:
    Chickpea (Cicer arietinum L.) is an important legume crop and widely cultivated in northwestern provinces of Iran. During a survey in the 2015 growing season a total of 170 selected chickpea plants with general yellowing symptoms including stunting and leaf bronzing were collected. Serological Elisa and tissue blot immunoassay (TIBA) tests revealed the presence of Bean leaf roll virus (BLRV) and Chickpea chlorotic stunt virus (CpCSV) as the predominant viruses in the region. Some serologically positive samples of BLRV and CpCSV were selected and rechecked by RT-PCR. Th e results of amplifi ed PCR products using a specifi c pair of primers towards the Cp gene region of the viruses were approximately 413 bp for CpCSV and 391 bp for BLRV. Results obtained from sequence comparison of BLRV (IR-F-Lor-5) isolate form two subgroups with eight other BLRV isolates from GeneBank indicating a high homology of 96% with isolates from Argentina, Germany, Tunisia, USA, Spain, and Colombia. An isolate from Norabad (Iran) (IR-Nor) had 98% homology with HQ840727 Libyan isolate. CpCSV sequence comparison with six other GeneBank isolates indicated 98% homology with isolates from Tunisia and Azerbaijan. Th e overall results of this research revealed the CpCSV and BLRV (luteoviruses) associated with the yellowing disease syndrome of chickpea crops in the surveyed region.
    Źródło:
    Journal of Plant Protection Research; 2017, 57, 2
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Phytoplasma detection in rose shoots propagated in vitro
    Autorzy:
    Kaminska, M
    Podwyszynska, M.
    Sliwa, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/58844.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    phytoplasma
    micropropagation
    rose
    antibiotic
    virus-like disease
    rose plant
    in vitro
    detection
    shoot
    Opis:
    The results of PCR examination indicated that during two years of tissue culture at standard conditions, on the medium with BAP 1 mg l-1 and continuous temperature of 20oC, phytoplasma could be detected in diseased plants of rose cv Sacha and Jazz. In the second year of micropropagation phytoplasma detection rate in tissues of infected roses increased and was relatively higher than in the first one. To test whether phytoplasmas are sensitive to temperature and light intensity, phytoplasma-affected micropropagated rose plants were grown on medium with BAP 1.0 or 0.5 mg l-1 and at the temperature of 4, 15, 20 or 25oC in darkness or in the light. PCR analysis indicated that phytoplasma detection was not effected by these conditions during 4 weeks of culturing. However, phytoplasma was not detectable in rose plants after 8 weeks culturing on the same medium without transplanting. Micropropagated rose shoots maintained on medium with Gentamycin or Baytril at the concentration of 25.0 or 50.0 mg l-1 had reduced growth and were chlorotic. However, no direct effect of applied antibiotics on phytoplasma detection was evidenced.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2005, 74, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection and molecular characterization of the Iris severe mosaic virus-Ir isolate from Iran
    Autorzy:
    Nateqi, M.
    Habibi, M.K.
    Dizadji, A.
    Parizad, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/66139.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    detection
    molecular characteristics
    iris severe mosaic virus
    isolate
    phylogenesis
    iris
    Iridaceae
    ornamental plant
    Iran
    Opis:
    Iris belongs to the Iridaceae family. It is one of the most important pharmaceutical and ornamental plants in the world. To assess the potyvirus incidence in natural resources of iris plants in Iran, Antigen Coated-Plate ELISA (ACP-ELISA) was performed on 490 symptomatic rhizomatous iris leaf samples, which detected the potyvirus in 36.7% of the samples. Genomic 3’ end of one mechanically non-transmitted potyvirus isolate, comprising a 3’ untranslated region (390 bp) and C-terminus of the coat protein (CP) gene (459 bp), was amplified by reverse transcription polymerase chain reaction (RT-PCR), which was ligated into pTG19-T vector. The nucleotide sequence of amplicons was compared with related sequences, using Blastn software available at NCBI GenBank, and showed the highest similarity with Iris severe mosaic virus (ISMV) isolates. The nucleotide and deduced amino acid sequence of the CP C-terminus region was more than 83% identical with other ISMV isolates, therefore this isolate was designated as ISMV-Ir. This new ISMV isolate is closely related to the Chinese ISMV-PHz in phylogenetic analysis, based on the partial nucleotide and deduced amino acid sequence of the CP region. This is the first report of ISMV occurrence on Iris spp. in Iran.
    Źródło:
    Journal of Plant Protection Research; 2015, 55, 3
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of RNA1 and RNA2 of Soil-borne wheat mosaic virus in winter wheat grown from infected seeds
    Autorzy:
    Jezewska, M.
    Trzmiel, K.
    Zarzynska-Nowak, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/66358.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    epidemiology
    detection
    RNA
    soil-borne wheat mosaic virus
    winter wheat
    wheat
    infected seed
    seed infection
    Opis:
    A Polish isolate of Soil-borne wheat mosaic virus (SBWMV-Pol1) was characterized by limited pathogenicity and a low concentration of virus particles in infected plant tissues. The aim of this research was to consider the possibility of seed-transmission dissemination of the virus. Seeds of winter wheat cv. Muszelka served as material for the studies. Two methods were involved in the diagnostics of seedlings grown from potentially infected seeds: enzyme-linked immunosorbent assay (ELISA), as the screening assay and immuno-capture-reverse transcription-polymerase chain reaction (IC-RT-PCR) for molecular confirmation of the infection. RNA1 and RNA2 of SBWMV-Pol1 were detected in 6 out of 1,410 plants submitted to diagnostic procedures. The possibility of seed transmission of SBWMV-Pol1 was discussed.
    Źródło:
    Journal of Plant Protection Research; 2016, 56, 4
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of infectious tobamoviruses in irrigation and drainage canals in Greater Poland
    Autorzy:
    Jezewska, M.
    Trzmiel, K.
    Zarzynska-Nowak, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/66243.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    detection
    reverse transcription polymerase chain reaction
    tobamovirus
    irrigation canal
    drainage canal
    virus
    water-borne virus
    Wielkopolska region
    Greater Poland zob.Wielkopolska region
    Opis:
    Water samples were collected from irrigation ditches and drainage canals surrounding fields in southern Greater Poland. Initially, the samples were subjected to low and highspeed centrifugation and obtained pellets were used to perform biological assays. Viral identification involved biological, electron microscopic as well as molecular methods. The occurrence of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) was demonstrated in 12 of the 17 examined water sources. The molecular analysis results showed TMV and ToMV co-infections in the analysed water samples. To our knowledge, this is the first report of tobamoviruses being found in environmental water in Poland.
    Źródło:
    Journal of Plant Protection Research; 2018, 58, 2
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Year-round blueberry scorch virus detection in highbush blueberry
    Wykrywanie wirusa oparzeliny borówki wysokiej w różnych okresach roku
    Autorzy:
    Paduch-Cichal, E.
    Chodorska, M.
    Kalinowska, E.
    Komorowska, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/11542904.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivar
    blueberry
    blueberry scorch virus
    scorch disease
    plant disease
    detection
    Northern highbush blueberry
    viral disease
    Opis:
    Viral diseases are a worldwide problem of blueberry which a major limiting factor for production. A survey for Blueberry scorch virus (BlScV) by DAS-ELISA in various organs of highbush blueberry conducted from May 2010 to April 2011, showed the occurrence of these virus in cvs Bluecrop and Herbert, which showing virus-like symptoms. Samples of plant materials (bud flower, flower, leaf, bark) were collected individually from each highbush blueberry plant of every cultivar. It was established that the detection of virus of each the investigated bushes cvs Bluecrop and Herbert depended on the tested plant materials as well as the period in which the tests were performed. The effectiveness of the virus detection varied for the investigated cultivars. The presence of the BlScV was confirmed in leaves samples with specific primer pair which amplifies a 430 bp fragment of the 5’-proximal ORF I [RNA-dependent RNA polymerase (RdRp)].
    Celem przeprowadzonych badań było wykrywanie i identyfikacja wirusa oparzeliny borówki wysokiej (Blueberry scorch virus, BlScV) w różnych organach pobieranych z krzewów borówki wysokiej odmian Bluecrop i Herbert rosnących na plantacji produkcyjnej zlokalizowanej w centralnej Polsce. Badania byáy prowadzone w okresie od maja 2010 do kwietnia 2011 r. przy użyciu testu serologicznego DAS-ELISA. Próby materiału roślinnego (pąki kwiatowe, kwiaty, liście, kora) pobierano indywidualnie z krzewów kaĪdej z badanych odmian. Ustalono, że wykrywanie wirusów w krzewach odmian Bluecrop i Herbert zależało od testowanego organu oraz terminu, w którym przeprowadzono test. Obecność BlScV w krzewach badanych odmian potwierdzono przy pomocy techniki RT-PCR z wykorzystaniem starterów amplifikujących fragment 5’ genu kodującego polimerazą RNA zależną od RNA.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 3; 3-11
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-12 z 12

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