- Tytuł:
- Effects of the polyhistidine tag on kinetics and other properties of trehalose synthase from Deinococcus geothermalis
- Autorzy:
-
Panek, Anna
Pietrow, Olga
Filipkowski, Paweł
Synowiecki, Józef - Powiązania:
- https://bibliotekanauki.pl/articles/1039568.pdf
- Data publikacji:
- 2013
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
trehalose
trehalose synthase
Escherichia coli
gene expression
Deinococcus geothermalis
influence of His6-tag - Opis:
- Two recombinant trehalose synthases from Deinococcus geothermalis (DSMZ 11300) were compared. A significant influence of the artificial polyhistidine tag was observed in protein constitution. The recombinant trehalose synthase from D. geothermalis with His6-tag has a higher Km value of 254 mM, in comparison with the wild-type trehalose synthase (Km 170 mM), and displayed a lower activity of maltose conversion when compared to the wild type. Moreover, differences in properties like temperature, pH, thermal- and pH-stability were observed. Presence of the histidine tag caused a decrease of thermal resistance in case of trehalose synthase with His6-tag. These data confirmed a suggestion that the introduction of the histidine domain produces in some seldom cases undesirable changes in the protein.
- Źródło:
-
Acta Biochimica Polonica; 2013, 60, 2; 163-166
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł