- Tytuł:
- Extraribosomal function of the acidic ribosomal P1-protein YP1α from Saccharomyces cerevisiae
- Autorzy:
-
Tchórzewski, Marek
Boldyreff, Brigitte
Grankowski, Nikodem - Powiązania:
- https://bibliotekanauki.pl/articles/1044447.pdf
- Data publikacji:
- 1999
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
acidic ribosomal P-protein
transactivation
translation
yeast - Opis:
- The yeast acidic ribosomal P-proteins YP1α, YP1β, YP2a and YP2b were studied for a possible transactivation potential beside their ribosomal function. The fusions of P-proteins with the GAL4 DNA-binding domain were assayed toward their transcriptional activity with the aid of reporter genes in yeast. Two of the P-proteins, YP1α and YP1β, exhibited transactivation potential, however, only YP1α can be regarded as a potent transactivator. This protein was able to transactivate a reporter gene associated with two distinct promoter systems, GAL1 or CYC1. Additionally, truncated proteins of YP1α and YP1β were analyzed. The N-terminal part of YP1α fused to GAL4-BD showed transactivation potential but the C-terminal part did not. Our results suggest a putative extraribosomal function for these ribosomal proteins which consequently may be classified as "moonlighting" proteins.
- Źródło:
-
Acta Biochimica Polonica; 1999, 46, 4; 901-910
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł