Temat: tissue culture - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Wpływ estru etylowego kwasu p-hydroksybenzoesowego na zdolność wbudowywania 32p do kwasów nukleinowych komórki zwierzęcej - w hodowli tkankowej
Vlijanie atilovogo afira p-gidrokibenaoinoi kisloty na cposobnost prisoedinenija 32 P k nukleinovym kislotam v aksplantacii zhivotnyh tkanei
The influence of ethyl p-hydroxybenzoate on the incorporation of 32p into nucleic acids of animal cells in tissue culture
Autorzy:: Fitak, B.
Lipinski, S.
Powiązania:: https://bibliotekanauki.pl/articles/873670.pdf
Data publikacji:: 1970
Wydawca:: Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:: estry etylowe
kwas p-hydroksybenzoesowy
kwasy nukleinowe
komorki zwierzece
hodowla tkankowa
zawartosc DNA
zawartosc RNA
ethyl ester
p-hydroxybenzoic acid
nucleic acid
animal cell
tissue culture
DNA content
RNA content
Źródło:: Roczniki Państwowego Zakładu Higieny; 1970, 21, 2
0035-7715
Pojawia się w:: Roczniki Państwowego Zakładu Higieny
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Wpływ metoksychloru na hodowlę tkankową nerki małpy Cercopithecus aethiops i płuca zarodka ludzkiego
Vlijanie metoksihlora nl kletki pocek martycszki Cercopithecus aethiops i kletki legkih celoveceskogo jambriona
The influence of methoxychlor on the tissue culture of the kidney of the monkey Cercopithecus aethiops and of the lung of human embryo
Autorzy:: Przezdziecki, Z.
Polna, I.
Powiązania:: https://bibliotekanauki.pl/articles/874038.pdf
Data publikacji:: 1970
Wydawca:: Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:: Cercopithecus aethiops
nerki
malpy
pluca
zarodek ludzki
hodowla tkankowa
metoksychlor
izomery
kidney
monkey
liver
embryo
tissue culture
metoxychlor
Źródło:: Roczniki Państwowego Zakładu Higieny; 1970, 21, 1
0035-7715
Pojawia się w:: Roczniki Państwowego Zakładu Higieny
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Rozmnazanie ziemniaka 'in vitro' w Instytucie Ziemniaka w Boninie
Autorzy:: Zaklukiewicz, K
Sekrecka, D.
Powiązania:: https://bibliotekanauki.pl/articles/835057.pdf
Data publikacji:: 1992
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: ziemniaki
kultury tkankowe
rozmnazanie roslin
hodowla in vitro
potato
tissue culture
plant propagation
in vitro culture
Źródło:: Ziemniak Polski; 1992, 3; 41-44
1425-4263
Pojawia się w:: Ziemniak Polski
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Bank genow 'in vitro' - stan obecny
Autorzy:: Zaklukiewicz, K
Sekrecka, D.
Powiązania:: https://bibliotekanauki.pl/articles/836124.pdf
Data publikacji:: 1993
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: hodowla roslin
ziemniaki
kultury tkankowe
hodowla in vitro
banki genow
odmiany roslin
plant breeding
potato
tissue culture
in vitro culture
gene bank
plant cultivar
Źródło:: Ziemniak Polski; 1993, 2; 28-36
1425-4263
Pojawia się w:: Ziemniak Polski
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium-Festuca complex
Autorzy:: Humphreys, M W
Thomas, H M
King, I P
Morgan, W G
Meredith, M R
Harper, J A
Humphreys, M O
Bettany, A J E
Dalton, S J
James, A R
Ougham, H J
Thomas, H
Powiązania:: https://bibliotekanauki.pl/articles/2046675.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: RNA
in situ
tissue culture
gene transfer
chromosome segment
anther culture
androgenesis
plant breeding
introgression mapping
fluorescence
hybridization
Lolium-Festuca complex
drought resistance
gene isolation
tetraploid hybrid
DNA
meiosis
grass
chromosome behaviour
Opis:: Recent advances at Institute of Grassland and Environmental Research (Aberystwyth, U.K.) in cytogenetics of the Lolium/Festuca complex places us in the advantageous position of being able to map genes of agronomic importance onto chromosome arms using fluorescence in situ hybridization (FISH). The ability to physically map genes leads to the capability for "dissecting" quantitative traits into their different components and will lead to better understanding of the complex physiological processes involved and the identification of their genetic control. By tagging genes of interest, using molecular and morphological markers, it will be possible to select and combine suites of desirable genes in a single genotype and thus produce novel cultivars by conventional breeding procedures. Programmes for introgression depend on the relationships between species and on levels of chromosome pairing. Phylogenetic relationships within the Lolium/Festuca complex are being determined using both genomic in situ hybridization (GISH) and FISH. With recent advances in genetic manipulation within the Lolium/Festuca complex, opportunities now arise for gene transfer from Lolium and Festuca species into other important agricultural crops.
Źródło:: Journal of Applied Genetics; 1997, 38, 3; 273-284
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Wheat anther culture as affected by various cultural changes and supplements
Autorzy:: Simonson, R L
Baenziger, P S
Gustafson, V D
Powiązania:: https://bibliotekanauki.pl/articles/2046627.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: cultural change
supplement
wheat
silver nitrate
Triticum aestivum
anther culture
cereal tissue
Opis:: With the numerous improvements in cereal tissue and wheat anther culture, it is necessary to determine which of the improvements should be combined for optimal response. This study was conducted using one highly responsive cultivar of wheat (Triticum aestivum L. cv. Pavon 76) to test the effectiveness of pre-culture cold treatment (0 or 3-7 days at 5°C) of anthers, five initiation basal media, and various changes in Murashige-Skoog regeneration media. A cold pre-culture treatment was inhibitory for all initiation media for embryoid initiation. Of the initiation media, P1, 85D12, and N6 were similar for embryoid initiation (0.80 to 0.90 embryoids/anther) without a cold pre-culture treatment. Plant regeneration was improved by the addition of amino acids or glucose, increased sucrose concentration, filter sterilizing the medium, and altering plant growth regulator concentrations. P1 medium which is normally used for embryoid initiation was also beneficial for improving plant regeneration. Ethylene inhibitors were generally not beneficial.
Źródło:: Journal of Applied Genetics; 1997, 38, 4; 381-392
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Application of in vitro culture techniques to barley [Hordeum vulgare L.] improvement
Autorzy:: Ullrich, S E
Kleinhofs, A.
Hou, L.
Jones, B.L.
Powiązania:: https://bibliotekanauki.pl/articles/2044453.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: tissue culture
doubled haploid
mutagenesis
breeding programme
anther culture
in vitro
callus culture
somaclonal variation
barley
mutation
Hordeum vulgare
embryo
molecular biology
Opis:: Several aspects of in vitro culture have potential for cereal improvement. This paper focuses on evaluation of somaclonal variation (SV) from immature embryo callus culture, and doubled haploid (DH) production via anther culture in barley. Genetically stable SV was observed for several seedling morphological traits such as albino, yellow, light green and lethal. SV occurred at approximately half the frequency of azide-induced mutagenesis. The potential for widespread application of anther culture-mediated DH production in barley breeding and genetic studies was increased through culture procedure improvements and understanding the inheritance of anther culture response. Methodology improvements included substitution of inexpensive gelrite for expensive ficoll or agarose, ability to grow anther donor plants under field as well as growth chamber conditions and flexibility in cold pretreatment/storage of anther donor spikes for 4-6 weeks prior to anther plating. From diallel analysis, inheritance of anther culture response was complex with additive and dominance effects for embryoid formation, total plant regeneration and green plant regeneration and reciprocal effects (maternal) for green plant regeneration. High x low responder crosses generated F₁’s that were intermediate in response and low x low crosses sometimes produced F₁ heterosis for green plant regeneration. Therefore, some recalcitrant types appear to be usable in anther culture DH production systems within a breeding program.
Źródło:: Journal of Applied Genetics; 1998, 39, 1; 49-58
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Somaclonal variation in winter wheat [Triticum aestivum L.]: frequency, occurrence and inheritance
Autorzy:: Cheng, X Y
Gao, M.W.
Liang, Z.Q.
Liu, G.Z.
Powiązania:: https://bibliotekanauki.pl/articles/2044461.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: inheritance
tissue culture
Triticum aestivum
occurrence
in vitro
winter wheat
gene mutation
plant breeding
callus induction
frequency
somaclonal variation
wheat
embryo
Opis:: Plants were regenerated from immature embryo cultures of 35 winter wheat genotypes. General responses of regenerated plants were investigated and a total of 7142 R₂ spike lines from 1593 R₁ plants were assessed in the field for somaclonal variants in 1985/86, 1986/87 and 1987/88. Selected variants were studied for their possible genetic inheritance. From regenerated plantlets, 81% survived and 63% produced fertile plants. Forms with reduced plant height, length of spike and other morphological abnormalities were found in this progeny. Populations of R₁ plants were highly variable due mainly to the physiological disturbances resulting from the in vitro process. Overall somaclonal variation frequencies were 14.2% per plant basis and 5.3% per R₂ spike basis. The variants were similar in the three different R₂ generations with predominant variants being negative in plant height, maturity, awns, spike type and plant type. Both uniform R₂ variant families and spike lines were found in addition to the segregating variants which constituted the majority. On average, in a variant family or line, 18% and 14% of their component lines and plants were variants, respectively. Inheritability was demonstrated for the uniform variant families and spike lines as well as segregated variants. Of those 134 selections, about 70% were classified as inheritable. Both recessive and dominant gene mutations at one, two or three loci were evident in some variants as suggested by the segregating data.
Źródło:: Journal of Applied Genetics; 1998, 39, 1; 59-72
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: The influence of stress conditions on acclimation of magnolia Magnolia x soulangiana Soul.-Bod.
Wpływ warunków stresu na aklimatyzację magnolii Magnolia x soulangiana Soul.-Bod.
Autorzy:: Kamenicka, A.
Lanakova, M.
Cerna, K.
Powiązania:: https://bibliotekanauki.pl/articles/808264.pdf
Data publikacji:: 2002
Wydawca:: Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:: stress condition
acclimation
magnolia
Magnolia x soulangiana
tissue culture
leaf cell
donor plant
Opis:: Under a scanning microscope, leaves from tissue cultures (after 8 weeks in vitro culturing), from 1 year old regenerants and from the 100 years old donor tree were studied. More abnormalities were observed in the tissue culture compared to regenerant and donor. During tissue culture transfer rapid, leaf dehydration occurs due to defective regulation mechanisms making stomata of tissue culture stay open. Epidermis as well as stomata cells were observed already on the leaves in in vitro conditions, but they become physiologically functioning only after transfer into conditions of natural environment.
Przy zastosowaniu mikroskopu scaningowego porównywano cechy budowy komórek liści pochodzących z 100 letniej rośliny matecznej, 8 tygodniowych kultur tkankowych oraz 1 rocznych regeneratów. W regenerantach z kultur tkankowych obserwowano więcej nienormalności w budowie komórek epidermy, komórek szparkowych i komórek przyszparkowych w porównaniu z komórkami pochodzącymi z rośliny matecznej. Podczas przenoszenia tkanek w kulturze in vitro obserwowano odwodnienie tkanek liści a mimo to komórki szparkowe pozostawały otwarte. Komórki epidermy oraz komórki szparkowe były obecne na liściach hodowli in vitro jednak stawały się one fizjologicznie funkcjonalne tylko wtedy gdy zostały przeniesione do warunków naturalnych.
Źródło:: Zeszyty Problemowe Postępów Nauk Rolniczych; 2002, 481, 1
0084-5477
Pojawia się w:: Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Nuclear morphology, polyploidy, and chromatin elimination in tissue culture of Allium fistulosum L.
Autorzy:: Joachimiak, A
Ilnicki, T.
Powiązania:: https://bibliotekanauki.pl/articles/59216.pdf
Data publikacji:: 2003
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: chromatin
heterochromatin
tissue culture
polyploidy
Alliaceae
morphology
Monocotyledoneae
micronucleus
Allium fistulosum
Opis:: The morphology of cell nuclei in callus obtained from root-tip meristems of Allium fistulosum L. (Monocotyledoneae, Alliaceae) was analysed. The most interesting phenomena observed in long-term callus culture were the different mechanisms of cell polyploidization, enlargement of telomeric segments of heterochromatin, and extensive chromatin elimination, associated with instability of nuclei size and DNA content. Protruding heterochromatin "spikes" were observed on the surface of some di- and polyploid nuclei. The presence of these spikes was connected with the formation of small heterochromatic micronuclei frequently found in the cytoplasm. It is suggested that these micronuclei are produced by direct elimination of heterochromatin from the interphase nuclei. Polyploid cells accumulated with each successive cell collection. The ploidy level attained by highly polyploid cells was 15C-220C. The shape of the nuclei and heterochromatin distribution suggest that polyploid nuclei in A. fistulosum tissue culture are produced by endoreduplication and by restitution cycles.
Źródło:: Acta Societatis Botanicorum Poloniae; 2003, 72, 1
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: The influence of PPM upon the sterility of the in vitro cultures in European beech [Fagus sylvatica L.]
Autorzy:: Kraj, W
Dolnicki, A.
Powiązania:: https://bibliotekanauki.pl/articles/56550.pdf
Data publikacji:: 2003
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: Plant Preservative Mixture
plant physiology
tissue culture
tree bud
Fagus sylvatica
sterilization
beech
sterility
in vitro
embryo
European beech
bud
Opis:: Investigations were conducted on the strongly infested by microbes embryos isolated from seed stratified in non-sterile conditions or from tree buds. The results showed, that PPM (Plant Preservative Mixture, produced by Plant Cell Technology, Inc.) was very useful for increasing the percentage of sterile cultures disinfected in a 5% solution of NaOCl. The best way of applying PPM turned out to be adding the substance directly to the growing medium. At the concentration of 2 or 4 cm3 x dm-3 of PPM there was an 30% increase in percentage of sterile of in vitro cultures developed from embryos and 70-80% of cultures developed from buds, as compared with the control without PPM. At the range of concentrations employed in this study, no negative effects of PPM upon the development and growth of callus were observed.
Źródło:: Acta Societatis Botanicorum Poloniae; 2003, 72, 4
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Embryogenic callus induction and differentiation in silver fir (Abies alba Mill.) tissue cultures
Autorzy:: Nawrot-Chorabik, K
Powiązania:: https://bibliotekanauki.pl/articles/41643.pdf
Data publikacji:: 2008
Wydawca:: Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:: silver fir
Abies alba
tissue culture
embryogenic suspensor mass
somatic embryogenesis
callus induction
differentiation
Opis:: The research was conducted on explants of silver fir (Abies alba Mill.) deriving from several forest districts in southern Poland. The study encompassed the influence of the origin of plant material, type of explants, kind of substances used for explants sterilization, PPM and the type of medium on the ability to form embryogenic callus and to develop somatic embryos in silver fir explants. From the plant material collected in three sites, 57 clones were obtained from mature zygotic embryos; this produced an embryogenesis frequency of 6%. Embryogenic callus was obtained with a diameter of 65–70 mm depending on the material origin. The best medium for development of callus inducted on embryos isolated from mature silver fir seeds was the SH medium. Somatic embryos were formed in a globular stadium (24 pieces) on this medium. The 10% solution of NaOCl (used for 15 minutes) turned out to be the most effective substance for seed sterilization.
Źródło:: Dendrobiology; 2008, 59; 31-40
1641-1307
Pojawia się w:: Dendrobiology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: A mathematical model of HIV-1 infection including the saturation effect of healthy cell proliferation
Autorzy:: Kouche, M.
Ainseba, B.
Powiązania:: https://bibliotekanauki.pl/articles/907704.pdf
Data publikacji:: 2010
Wydawca:: Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:: zakażenie HIV
stabilność
hodowla tkanek
HIV
periodic oscillations
persistence
stability
tissue culture
Opis:: In this paper we derive a model describing the dynamics of HIV-1 infection in tissue culture where the infection spreads directly from infected cells to healthy cells trough cell-to-cell contact. We assume that the infection rate between healthy and infected cells is a saturating function of cell concentration. Our analysis shows that if the basic reproduction number does not exceed unity then infected cells are cleared and the disease dies out. Otherwise, the infection is persistent with the existence of an infected equilibrium. Numerical simulations indicate that, depending on the fraction of cells surviving the incubation period, the solutions approach either an infected steady state or a periodic orbit.
Źródło:: International Journal of Applied Mathematics and Computer Science; 2010, 20, 3; 601-612
1641-876X
2083-8492
Pojawia się w:: International Journal of Applied Mathematics and Computer Science
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of Brassica oleracea var. botrytis (cauliflower)
Autorzy:: Siong, P.K.
Taha, R.M.
Rahiman, F.A.
Powiązania:: https://bibliotekanauki.pl/articles/19314.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: somatic embryogenesis
plant regeneration
hypocotyl
leaf explant
Brassica oleracea var.botrytis
cauliflower
embryogenic callus
callus
tissue culture
Opis:: We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23–25) than through the direct pathway (14–19). The number of embryos produced was high. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones.
Źródło:: Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
0001-5296
Pojawia się w:: Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants
Autorzy:: Li, J.
Kang, Y.
Qiang, S.
Peng, G.
Powiązania:: https://bibliotekanauki.pl/articles/56457.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: propagation
goldenrod
Solidago canadensis
leaf
nodal explant
callus formation
micropropagation
adventitious shoot
tissue culture
Opis:: Goldenrod (Solidago canadensis L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog's medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological variation when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation, rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed.
Źródło:: Acta Societatis Botanicorum Poloniae; 2012, 81, 1
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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