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    Wyświetlanie 1-6 z 6
    Tytuł:
    Genotypic discrimination of Aspergillus fumigatus strain from related species within section fumigati
    Autorzy:
    Giray, Betül
    Kocaöğüt, Elif
    Uçar, Füsun B.
    Haliki-Uztan, Alev
    Powiązania:
    https://bibliotekanauki.pl/articles/989031.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    roda
    βtub
    a. fumigatus
    its-pcr
    antifungal susceptibility testing
    Opis:
    Introduction and objective. The aim was to make an exact diagnosis of 20 strains using molecular biological methods which were isolated from the atmosphere of the inpatient rooms in the Oncology and other departments of the Ege University Medical Faculty Hospital, and identified as Aspergillus fumigatus through phenotypic tests, and to determine their antibiotic susceptibility patterns. Materials and method. It was confirmed that the 20 phenotypically-identified A. fumigatus strains belonged to the section Fumigati after they were tested by the ITS-PCR method. Their sequence analysis was performed and the results sent to the NCBI GenBank, and their accession numbers were obtained. For their exact diagnosis at the species level, the β-tub (β-tubulin) and rodA (RodletA) genes were examined with the multiplex PCR. Anti-fungal susceptibility of the 20 strains was determined according to the M38-A2 standards. Results. As a result of ITS-PCR, it was confirmed that 19 of the 20 strains identified as A. fumigatus through the phenotypic methods belonged to the section Fumigati. However, after the detection of β-tub and rodA genes, all 20 strains were identified as A. fumigatus. Conclusion. Although the results of the phenotypic and molecular biological tests applied to filamentous fungi do not often overlap, in this study, the results obtained from the molecular analysis confirmed the results of the phenotypic tests. However, 1 of the 20 strains phenotypically-identified as A. fumigatus was identified as Penicillium spp. as a result of ITSPCR and sequence analysis. On the other hand, the profile obtained from β-tub and rodA tests indicated that the strain was A. fumigatus. Based on these results, this strain is thought to belong to the Aspergilloides genus which has the features of both genera.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2016, 23, 3
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification modeling of Polish electric power exchange
    Autorzy:
    Marlęga, R.
    Tchórzewski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/94879.pdf
    Data publikacji:
    2016
    Wydawca:
    Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
    Tematy:
    parametrical model
    identification modeling
    simulation research
    susceptibility testing
    MATLAB
    Simulink environment
    Opis:
    This paper contains selected results of identification modeling of Polish Electric Power Exchange (EPE). In order to obtain EPE system model it was performed identification based on figures of EPEís Day-Ahead Market. During performing identification process, parametric arx model in System Identification Toolbox environment was utilized. Generated EPE parametric model has been further used for performing simulation tests and realization of susceptibility testing. Suitable models were implemented in Simulink software. As a result of simulation and susceptibility testing, many interesting findings has been delivered.
    Źródło:
    Information Systems in Management; 2016, 5, 2; 195-204
    2084-5537
    2544-1728
    Pojawia się w:
    Information Systems in Management
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Relation of the polymorphism of cyp51A sequence and the susceptibility of Aspergillus fumigatus isolates to triazoles determined by commercial gradient test (Etest) and by reference methods
    Autorzy:
    Nawrot, Urszula
    Sulik-Tyszka, Beata
    Kurzyk, Ewelina
    Mroczyńska, Martyna
    Włodarczyk, Katarzyna
    Wróblewska, Marta
    Basak, Grzegorz
    Brillowska-Dąbrowska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1038548.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Aspergillus fumigatus
    triazole resistance
    susceptibility testing
    Etest
    cyp51A sequence
    Opis:
    The aim of this study was to evaluate the accuracy of commercial gradient test (Etest) in the detection of triazole resistant Aspergillus fumigatus isolates using reference microdilution methods and the analysis of sequences of the cyp 51A gene. The study was performed on twenty clinical isolates which were identified as Aspergillus fumigatus based on the DNA sequences of the ITS1-2 fragment of ribosomal DNA and the β-tubulin gene, out of them seventeen isolates showed wild-type cyp51A sequence and three were positive for the mutation TR34/L98H. All isolates were tested for the susceptibility to itraconazole (ITZ), voriconazole (VOR) and posaconasole (POS) using microdilution methods, according to EUCAST and CLSI protocols, as well as using Etest. The results of microdilution and Etests were analysed separately according to clinical breakpoints (CBP) defined by EUCAST version 7.0 and epidemiological cut off values (ECV). Etest as well as reference methods excellently recognised the WT isolates, which were susceptible to all tested triazoles, regardless of the method and CBP or ECV criteria used. The Etest recognized three non-WT isolates as resistant or intermediately sensitive to ITZ and POS and one as resistant to VOR. The categorical concordance between Etests and EUCAST and Etests and the CLSI method ranged from 90 to 100%. The interpretation of the results obtained from routine A. fumigatus Etests requires great caution. The use of the confirmative examinations with reference AST methods as well as with molecular tests is recommended.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 4; 631-634
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Środowisko a antybiotykooporność izolatów Escherichia coli
    Envirnomental dissemision of resistance among Escherichia coli isolates
    Autorzy:
    Krzyśko-Łupicka, T.
    Kręcidło, M.
    Mysłek, M.
    Kręcidło, Ł.
    Powiązania:
    https://bibliotekanauki.pl/articles/126404.pdf
    Data publikacji:
    2017
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    metoda Kirby-Bauera
    testowanie antybiotykoodporności
    Escherichia coli
    Kirby-Bauera method
    antibiotic susceptibility testing
    Opis:
    Pula farmaceutyków w środowisku powiększa się w wyniku kontaminacji wód, gruntów, ścieków, a także surowców pochodzenia zwierzęcego. Obecność antybiotyków w środowisku wpływa nie tylko na zahamowanie rozwoju mikroorganizmów, ale w stężeniu subinhibicyjnym może stymulować ekspresję genów wywołujących zjawisko antybiotykooporności. Celem pracy było porównanie wrażliwości na antybiotyki pałeczek Escherichia coli wyizolowanych z surowego mleka i zmieszanych odpadów drobiarskich. Materiał badawczy stanowiły szczepy E.coli wyizolowane z surowca mleczarskiego i zmieszanych odpadów drobiarskich. Ocenę wrażliwości izolatów na antybiotyki przeprowadzono metodą Kirby-Bauera. Profil oporności E. coli ustalano na podstawie rekomendacji CLSI. Wykorzystano krążki firmy BTL wysycone takimi antybiotykami, jak: ampicylina (AM10), chloramfenikol (C30), gentamycyna (CN10), tetracyklina (TE30) oraz mieszaniną sulfmetoksazolu i trimetoprimu (SXT25). Izolaty E. coli z surowca mleczarskiego charakteryzowały się większą opornością na ampicylinę i wyższą wrażliwością na pozostałe badane antybiotyki w porównaniu do izolatów z odpadów drobiarskich.
    Recently amount of pharmaceutical substances in the environment is getting higher due to spreading and relocation of water and soil contamination by sources such as a livestock production or sewage. The persistence of antibiotic in the environment may affect both the inhibition of microbial growth and also the stimulation of resistance gens expression, when antibiotics are in the subinhibitory concentration. The purpose of a study was to compare susceptibility of Escherichia coli isolates from two following sources: a raw milk from dairy and a poultry waste. The assessment of susceptibility on antibiotic was carried out by the Kirby-Bauer’s method. The resistance profile of isolates was estimated with using of CLSI standards. Antibiotic discs from BTL company were tested with particular type of substances: ampicillin (AM10), chloramphenicol (C30), gentamicin (CN10), tetracycline (TE30) and solution of sulfamethoxazole and trimethoprim (SXT25). Isolates of E. coli from raw milk were more resistant to ampicillin and characterised by higher susceptibility to the other antibiotics in the comparison to the isolates from poultry waste.
    Źródło:
    Proceedings of ECOpole; 2017, 11, 2; 517-523
    1898-617X
    2084-4557
    Pojawia się w:
    Proceedings of ECOpole
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro antifungal susceptibility testing of Scopulariopsis brevicaulis strains using agar diffusion method
    Ocena in vitro lekowrażliwości szczepów Scopulariopsis brevicaulis metodą dyfuzji w agarze
    Autorzy:
    Skora, M.
    Macura, A.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/841599.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    in vitro
    antifungal susceptibility
    testing
    Scopulariopsis brevicaulis
    agar diffusion test
    human infection
    Opis:
    The genus Scopulariopsis is a common soil saprotroph and has been isolated from air, organic waste and also from plant, animal and human tissues. Scopulariopsis has mainly been associated in humans with superficial mycoses, but it has also been described as the cause of subcutaneous and invasive infections. The most common aetiological agent of infections in humans is Scopulariopsis brevicaulis. This species has been reported to be resistant in vitro to broad-spectrum antifungal agents available today. The aim of the study was to establish in vitro antifungal susceptibility of 35 S. brevicaulis strains against amphotericin B (AMB), flucytosine (FC), caspofungin (CAS), terbinafine (TER), ciclopirox (CIC), voriconazole (VOR), clotrimazole (CTR), miconazole (MCZ), econazole (ECO), ketoconazole (KET), itraconazole (ITR), and fluconazole (FLU). Antifungal susceptibility tests were evaluated by an agar diffusion method (Neo-Sensitabs, Rosco, Denmark). AMB, FC, CAS, ITR and FLU showed no antifungal activity against S. brevicaulis. TER, CIC, CTR, KET, VOR, ECO, and MCZ revealed inhibitory activity for S. brevicaulis, but it varied for each of the drugs. The best antifungal effect was observed for TER and CIC. All isolates had large inhibition zones for TER and CIC. CTR was also inhibitory for all tested S. brevicaulis isolates, but the diameters of inhibition zones were smaller than for TER and CIC. Nearly 89% isolates showed inhibition zones for KET and the mean diameter of the inhibition zone was comparable to CTR. The least antifungal activity exhibited VOR, ECO and MCZ. Because of the multiresistance of S. brevicaulis, infections due to this species may not respond to particular antifungal treatment and other therapeutic approaches should be considered e.g., combined therapy and/or surgery.
    Źródło:
    Annals of Parasitology; 2011, 57, 2
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro antifungal susceptibility testing of Scopulariopsis brevicaulis strains using agar diffusion method
    Ocena in vitro lekowrażliwości szczepów Scopulariopsis brevicaulis metodą dyfuzji w agarze
    Autorzy:
    Skóra, M.
    Macura, A.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143334.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    in vitro
    antifungal susceptibility
    testing
    Scopulariopsis brevicaulis
    agar diffusion test
    human infection
    Opis:
    The genus Scopulariopsis is a common soil saprotroph and has been isolated from air, organic waste and also from plant, animal and human tissues. Scopulariopsis has mainly been associated in humans with superficial mycoses, but it has also been described as the cause of subcutaneous and invasive infections. The most common aetiological agent of infections in humans is Scopulariopsis brevicaulis. This species has been reported to be resistant in vitro to broad-spectrum antifungal agents available today. The aim of the study was to establish in vitro antifungal susceptibility of 35 S. brevicaulis strains against amphotericin B (AMB), flucytosine (FC), caspofungin (CAS), terbinafine (TER), ciclopirox (CIC), voriconazole (VOR), clotrimazole (CTR), miconazole (MCZ), econazole (ECO), ketoconazole (KET), itraconazole (ITR), and fluconazole (FLU). Antifungal susceptibility tests were evaluated by an agar diffusion method (Neo-Sensitabs, Rosco, Denmark). AMB, FC, CAS, ITR and FLU showed no antifungal activity against S. brevicaulis. TER, CIC, CTR, KET, VOR, ECO, and MCZ revealed inhibitory activity for S. brevicaulis, but it varied for each of the drugs. The best antifungal effect was observed for TER and CIC. All isolates had large inhibition zones for TER and CIC. CTR was also inhibitory for all tested S. brevicaulis isolates, but the diameters of inhibition zones were smaller than for TER and CIC. Nearly 89% isolates showed inhibition zones for KET and the mean diameter of the inhibition zone was comparable to CTR. The least antifungal activity exhibited VOR, ECO and MCZ. Because of the multiresistance of S. brevicaulis, infections due to this species may not respond to particular antifungal treatment and other therapeutic approaches should be considered e.g., combined therapy and/or surgery.
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 2; 111-117
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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