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    Wyświetlanie 1-8 z 8
    Tytuł:
    Structural basis of the interspecies interaction between the chaperone DnaK(Hsp70) and the co-chaperone GrpE of archaea and bacteria
    Autorzy:
    Żmijewski, Michał
    Skórko-Glonek, Joanna
    Tanfani, Fabio
    Banecki, Bogdan
    Kotlarz, Agnieszka
    Macario, Alberto
    Lipińska, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1041069.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    substrate-binding domain
    DnaK-GrpE complex
    archaeal Hsp70(DnaK)
    archaeal DnaK structure
    molecular chaperones
    ATPase domain
    Opis:
    Hsp70s are chaperone proteins that are conserved in evolution and present in all prokaryotic and eukaryotic organisms. In the archaea, which form a distinct kingdom, the Hsp70 chaperones have been found in some species only, including Methanosarcina mazei. Both the bacterial and archaeal Hsp70(DnaK) chaperones cooperate with a GrpE co-chaperone which stimulates the ATPase activity of the DnaK protein. It is currently believed that the archaeal Hsp70 system was obtained by the lateral transfer of chaperone genes from bacteria. Our previous finding that the DnaK and GrpE proteins of M. mazei can functionally cooperate with the Escherichia coli GrpE and DnaK supported this hypothesis. However, the cooperation was surprising, considering the very low identity of the GrpE proteins (26%) and the relatively low identity of the DnaK proteins (56%). The aim of this work was to investigate the molecular basis of the observed interspecies chaperone interaction. Infrared resolution-enhanced spectra of the M. mazei and E. coli DnaK proteins were almost identical, indicating high similarity of their secondary structures, however, some small differences in band position and in the intensity of amide I' band components were observed and discussed. Profiles of thermal denaturation of both proteins were similar, although they indicated a higher thermostability of the M. mazei DnaK compared to the E. coli DnaK. Electrophoresis under non-denaturing conditions demonstrated that purified DnaK and GrpE of E. coli and M. mazei formed mixed complexes. Protein modeling revealed high similarity of the 3-dimensional structures of the archaeal and bacterial DnaK and GrpE proteins.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 2; 245-252
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The DnaK chaperones from the archaeon Methanosarcina mazei and the bacterium Escherichia coli have different substrate specificities
    Autorzy:
    Żmijewski, Michal
    Skórko-Glonek, Joanna
    Tanfani, Fabio
    Banecki, Bogdan
    Kotlarz, Agnieszka
    Macario, Alberto
    Lipińska, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1040934.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    archaeal DnaK quaternary structure
    archaeal Hsp70(DnaK)
    substrate-binding by archaeal DnaK
    Opis:
    Hsp70 (DnaK) is a highly conserved molecular chaperone present in bacteria, eukaryotes, and some archaea. In a previous work we demonstrated that DnaK from the archaeon Methanosarcina mazei (DnaKMm) and the DnaK from the bacterium Escherichia coli (DnaKEc) were functionally similar when assayed in vitro but DnaKMm failed to substitute for DnaKEc in vivo. Searching for the molecular basis of the observed DnaK species specificity we compared substrate binding by DnaKMm and DnaKEc. DnaKMm showed a lower affinity for the model peptide (a-CALLQSRLLS) compared to DnaKEc. Furthermore, it was unable to negatively regulate the E. coli σ32 transcription factor level under heat shock conditions and poorly bound purified σ32, which is a native substrate of DnaKEc. These observations taken together indicate differences in substrate specificity of archaeal and bacterial DnaKs. Structural modeling of DnaKMm showed some structural differences in the substrate-binding domains of DnaKMm and DnaKEc, which may be responsible, at least partially, for the differences in peptide binding. Size-exclusion chromatography and native gel electrophoresis revealed that DnaKMm was found preferably in high molecular mass oligomeric forms, contrary to DnaKEc. Oligomers of DnaKMm could be dissociated in the presence of ATP and a substrate (peptide) but not ADP, which may suggest that monomer is the active form of DnaKMm.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 3; 509-522
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Conserved Cys residue influences catalytic properties of potato endo-(1→3)-β-glucanase GLUB20-2
    Autorzy:
    Witek, Agnieszka
    Witek, Kamil
    Hennig, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/1040688.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    GLUB20-2
    inhibition
    endo-(1→3)-β-glucanase
    Solanum tuberosum
    structure-function relationships
    substrate specificity
    Opis:
    The synthesis and degradation of (1→3)-β-glycosidic bonds between glucose moieties are essential metabolic processes in plant cell architecture and function. We have found that a unique, conserved cysteine residue, positioned outside the catalytic centre of potato endo-(1→3)-β-glucanase - product of the gluB20-2 gene, participates in determining the substrate specificity of the enzyme. The same residue is largely responsible for endo-(1→3)-β-glucanase inhibition by mercury ions. Our results confirm that the spatial adjustment between an enzyme and its substrate is one of the essential factors contributing to the specificity and accuracy of enzymatic reactions.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 791-797
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Recent advances in studies on biochemical and structural properties of equilibrative and concentrative nucleoside transporters
    Autorzy:
    Podgorska, Marzena
    Kocbuch, Katarzyna
    Pawelczyk, Tadeusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1041310.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    nucleoside transporters
    structure
    gene locus
    substrate specificity
    tissue distribution
    adenosine
    Opis:
    Nucleoside transporters (NT) facilitate the movement of nucleosides and nucleobases across cell membranes. NT-mediated transport is vital for the synthesis of nucleic acids in cells that lack de novo purine synthesis. Some nucleosides display biological activity and act as signalling molecules. For example, adenosine exerts a potent action on many physiological processes including vasodilatation, hormone and neurotransmitter release, platelet aggregation, and lipolysis. Therefore, carrier-mediated transport of this nucleoside plays an important role in modulating cell function, because the efficiency of the transport processes determines adenosine availability to its receptors or to metabolizing enzymes. Nucleoside transporters are also key elements in anticancer and antiviral therapy with the use of nucleoside analogues. Mammalian cells possess two major nucleoside transporter families: equilibrative (ENT) and concentrative (CNT) Na+-dependent ones. This review characterizes gene loci, substrate specificity, tissue distribution, membrane topology and structure of ENT and CNT proteins. Regulation of nucleoside transporters by various factors is also presented.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 4; 749-758
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Experimental research on the filtration properties of an untamped mixture with "PMM" polymer mineral material
    Autorzy:
    Mikayelyan, Nver A.
    Tokmajyan, Vache H.
    Vartanyan, Arevshad A.
    Mkrtchyan, Samvel H.
    Galstyan, Armavir G.
    Powiązania:
    https://bibliotekanauki.pl/articles/31342518.pdf
    Data publikacji:
    2023
    Wydawca:
    Politechnika Częstochowska
    Tematy:
    filtration
    substrate
    humidity
    hydraulic structure
    filtracja
    podłoże
    wilgotność
    struktura hydrauliczna
    Opis:
    Polymermineral material "PMM" has multiple cycles of swelling and drying. It does not decompose under soil, biological and atmospheric influences and is environmentally safe. By adding the proportion of up to 30 g/kg of PMM additive to soil and tamping, we get a waterproofing material that can be used in urban construction and water supply. Taking into account the fact it is not always technically possible to carry out high-quality ramming when repairing channel walls, the task was to conduct an experimental study in order to determine the total moisture content of the mixture of PMM additive with soil. Experimental results of laboratory studies show that when PMM is added to the soil at a ratio of 30 g/kg under evaporation conditions, the mass of excess water in the substrate increases with time.
    Źródło:
    Budownictwo o Zoptymalizowanym Potencjale Energetycznym; 2023, 12; 17-24
    2299-8535
    2544-963X
    Pojawia się w:
    Budownictwo o Zoptymalizowanym Potencjale Energetycznym
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    An obtaining of nanoheteroepitaxial structures with quantum dots for high effective photovoltaic devices, investigation of their properties
    Poluchenie nanogeteroehpitaksialnykh struktur s kvantovymi tochkami dlja vysokoehffektivnykh solnechnykh ehlementov, issledovanie ikh svojjstv
    Autorzy:
    Maronchuk, I.
    Bykovsky, S.
    Bondarec, S.
    Velchenko, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/792288.pdf
    Data publikacji:
    2014
    Wydawca:
    Komisja Motoryzacji i Energetyki Rolnictwa
    Tematy:
    nanoheteroepitaxial structure
    quantum dot
    liquid phase
    substrate
    photovoltaic device
    investigation
    property
    semiconductor compound
    Źródło:
    Teka Komisji Motoryzacji i Energetyki Rolnictwa; 2014, 14, 1
    1641-7739
    Pojawia się w:
    Teka Komisji Motoryzacji i Energetyki Rolnictwa
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Morfometryczna charakterystyka struktury podłoży ogrodniczych
    Morphometric characteristic of the structure of horticultural substrates
    Autorzy:
    Jaroszuk-Sierocinska, M.
    Słowinska-Jurkiewicz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/36530.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Instytut Agrofizyki PAN
    Tematy:
    podloza ogrodnicze
    struktura
    analiza morfometryczna
    analiza obrazu
    horticultural substrate
    substrate structure
    morphometric analysis
    image analysis
    Opis:
    Przeprowadzono morfometryczną analizę struktury 8 podłoży ogrodniczych wykorzystywanych do uprawy roślin w pojemnikach. Oznaczono podstawowe parametry stosowane w komputerowej analizie obrazu: makroporowatość (powierzchnię względną przekrojów porów o wymiarach powyżej 43 μm), liczebność przekrojów porów, średnią powierzchnię przekrojów porów, długość linii granicznej między przekrojami porów i elementów fazy stałej oraz średni rozmiar przekrojów porów. Wartość makroporowatości pozwala na ocenę zdolności podłoża do retencji wody najłatwiej dostępnej dla roślin.
    A morphometric structure analysis was made of 8 horticultural substrates applied for plant cultivation in containers. The basic parameters applied in computer image analysis were investigated, i.e. macroporosity (relative area of cross-section of pores with sizes above 43 μm), number and mean area of pores cross-sections, length of limit line between pore cross-sections and structural elements of solid phase, mean intercept length of pore cross-sections. Value of macroporosity allows the assessment of the substrate ability for retention of easily available water for plants.
    Źródło:
    Acta Agrophysica; 2011, 17, 1[188]
    1234-4125
    Pojawia się w:
    Acta Agrophysica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of stress based NDT methods for concrete repair bond quality control
    Autorzy:
    Garbacz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/200925.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    concrete structure
    repair durability
    repair quality control
    NDT techniques
    substrate quality
    interface quality
    struktura betonu
    naprawy kontroli jakości
    techniki badań nieniszczących
    jakość podłoża
    interfejs podłoża
    Opis:
    Adhesion in repair systems is one of the most important factors affecting their durability. Elaboration of a reliable nondestructive test method to perform an adhesion mapping is one of the most important tasks. A majority of NDT methods applicable for the assessment of concrete structures are based on the propagation of various types of stress waves. In this paper, the influence of the repair material type (polymer-cement or polymer) and quality of the concrete substrate (roughness, microcracking, not cleaned surface) upon propagation of stress waves in a repair system was studied in view of developing a reliable NDT procedure for the field assessment of bond quality in concrete repairs.
    Źródło:
    Bulletin of the Polish Academy of Sciences. Technical Sciences; 2015, 63, 1; 77-85
    0239-7528
    Pojawia się w:
    Bulletin of the Polish Academy of Sciences. Technical Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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