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Wyszukujesz frazę "subcellular distribution" wg kryterium: Temat


Wyświetlanie 1-2 z 2
Tytuł:
Abscisic acid does not influence the subcellular distribution of the HYL1 protein from Arabidopsis thaliana
Autorzy:
Lesicka-Górecka, Joanna
Szarzyńska, Bogna
Sawczak, Marta
Bagdiul, Ivona
Górski, Paweł
Jarmołowski, Artur
Szweykowska-Kulińska, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1040709.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
subcellular distribution
abscisic acid
Arabidopsis thaliana
expression profile
HYL1 protein
Opis:
HYL1 is a nuclear protein involved in the processing of miRNAs but its exact function remains unknown. Arabidopsis thaliana hyl1 mutants exhibit hypersensitivity to ABA. We decided to answer the question whether ABA affects the HYL1 protein localization within the cell and show that it does not. We also studied the expression of HYL1 in different tissues and organs. In this paper we show for the first time the expression profile of the HYL1 protein using anti-HYL1 antibodies. The protein is present in seedlings and mature plants in all organs studied, with the highest amount in inflorescences. A. thaliana HYL1 protein has several repetitions of a 28-amino-acid sequence at the C-terminus that confer protein instability. Our bioinformatic analysis of HYL1 homologs in different Brassica species shows that this repetition is typical only for Arabidopsis. This may suggest a relatively late evolutionary acquisition of the C-terminal domain.
Źródło:
Acta Biochimica Polonica; 2008, 55, 3; 517-524
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nuclear Targeting Study of HPMA Copolymer-5-Fluorouracil Conjugate Based on the Subcellular Distribution in PC-3 Cells
Autorzy:
You, Lan
Dai, Shiying
Huang, Yuan
Ye, Liming
Fu, Chunmei
Powiązania:
https://bibliotekanauki.pl/articles/895627.pdf
Data publikacji:
2019-04-30
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
HPLC
analysis
Drug targeting
subcellular distribution
polymeric drug delivery systems
Opis:
In recent decades, a large number of targeting drug delivery systems have been designed and evaluated for cancer therapy. An important part of these studies is to monitor the subcellular fate of macromolecules. Fluorescence labeling technique was the most adopted method. However, it had the disadvantages of alteration the properties of drugs as well as artifactual redistribution of fluorescent markers caused by fixation. In the present study, a more accurate method, HPLC analysis preceded by subcellular fractionation was developed and validated to study the distribution of a newly synthesized tumor cell targeting drug delivery system, HPMA copolymer-5-FU conjugates (P-FU) in PC-3 cells. P-FU was proved to accumulate more in nuclei than free 5-FU, whose concentration in the culture medium was approximately 1/20 that of 5-FU when attaining similar nucleus drug concentration. On the contrary, the overwhelming majority of drugs accumulated in nonnucleus when PC-3 cells were treated with free 5-FU. These results indicated that P-FU had nuclear targeting effect. The developed procedure allows an accurate subcellular fate screening for many macromolecules.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2019, 76, 2; 205-213
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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