Temat: stirred tank bioreactor - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Studies on the effect of coal particle size on biodepyritization of high sulfur coal in batch bioreactor
Autorzy:: Singh, S.
Srichandan, H.
Pathak, A.
Gahan, Ch. S.
Lee, S.
Kim, D. J.
Kim, B. G.
Powiązania:: https://bibliotekanauki.pl/articles/778523.pdf
Data publikacji:: 2015
Wydawca:: Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:: biodepyritization
coal
pyrite
stirred tank bioreactor
Opis:: The moderate thermophilic mix culture bacteria were used to depyritize the Illinois coal of varying particle sizes (-100 μm, 100-200 μm, +200 μm). Mineral libration analysis showed the presence of pyrite along with other minerals in coal. Microbial depyritization of coal was carried out in stirred tank batch reactors in presence of an iron-free 9K medium. The results indicate that microbial depyritization of coal using moderate thermophiles is an efficient process. Moreover, particle size of coal is an important parameter which affects the efficiency of microbial depyritization process. At the end of the experiment, a maximum of 75% pyrite and 66% of pyritic sulphur were removed from the median particle size. The XRD analysis showed the absence of pyrite mineral in the treated coal sample. A good mass balance was also obtained with net loss of mass ranging from 5-9% showing the feasibility of the process for large scale applications.
Źródło:: Polish Journal of Chemical Technology; 2015, 17, 1; 97-102
1509-8117
1899-4741
Pojawia się w:: Polish Journal of Chemical Technology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Impact of bioreactor scale on lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture
Autorzy:: Pawlak, M.
Bizukojć, M.
Ledakowicz, S.
Powiązania:: https://bibliotekanauki.pl/articles/185113.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: lowastatyna
bioreaktor
proces wsadowy
lovastatin
Aspergillus terreus ATCC 20542
shake flask
stirred tank bioreactor
batch process
Opis:: Biosynthesis of lovastatin (a polyketide metabolite of Aspergillus terreus) in bioreactors of different working volume was studied to indicate how the change of scale of the process influences the formation of this metabolite. The experiments conducted in shake flasks of 150 ml working volume allowed to obtain lovastatin titres at the level of 87.5 mg LOV l-1, when two carbon sources, namely lactose and glycerol were used. The application of the same components in a large stirred-tank bioreactor of 5.3-litre working volume caused a decrease of lovastatin production by 87% compared to the shake flask culture. The deficiency of nitrogen in this bioreactor did not favour the formation of lovastatin, in contrast to the small bioreactor of 1.95-litre working volume, in which lovastatin titres comparable to those in the shake flasks could be achieved, when organic nitrogen concentration was two-fold decreased. When the control of pH and/or pO2 was used simultaneously, an increase in lovastatin production was observed in the bioreactors. However, these results were still slightly lower than lovastatin titres obtained in the shake flasks.
Źródło:: Chemical and Process Engineering; 2012, 33, 1; 71-84
0208-6425
2300-1925
Pojawia się w:: Chemical and Process Engineering
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Hodowla alg Chlorella sp. w bioreaktorze zbiornikowym z mieszaniem mechanicznym
Cultivation of green algae Chlorella sp. in a stirred tank bioreactor
Autorzy:: Makowska, M.
Powiązania:: https://bibliotekanauki.pl/articles/296974.pdf
Data publikacji:: 2017
Wydawca:: Politechnika Częstochowska. Wydawnictwo Politechniki Częstochowskiej
Tematy:: algae Chlorella sp.
algae biomass
biogenic compounds
optical density
stirred tank bioreactor
algi Chlorella sp.
biomasa alg
związki biogenne
gęstość optyczna
bioreaktor z mieszaniem mechanicznym
Opis:: Algae, as a renewable raw materials, are valuable sources of biomass for many branches of industry. In the area of environmental protection they are used among others for biological treatment of water, removal and recovery of heavy metals from industrial wastewater, production of bio-fertilizers and fodder additives. Due to their rapid growth, they are able to efficiently use less fertile areas more than energetic plants (e.g. rapeseed, soya). To properly grow, under in vitro conditions, algae need access to water, nutrients (i.a. nitrogen and phosphorus) and carbon dioxide as well as appropriate temperature, pH, mixing and lighting. In our climate conditions, algal cultivation in natural water reservoirs is ineffective, due to daily and annual temperature and sunlight changes. An efficient alternative is the use of bioreactors that limit or eliminate the possibility of bacterial infection in algae culture. The aim of the study was to investigate the biomass yield of algae Chlorella sp., cultivated in BG-11 synthetic medium, inside the tank bioreactor, under the artificial lighting and aeration conditions. The periodic culture was conducted at 25°C with mechanical stirring (150 rpm). Compressed air was supplied to the suspension (in alternating cycles: 5 successive aeration days, 2 days without aeration). The process of algal cell multiplication was supported by the light exposure for 16 hours per day (8 h - dark phase). The source of light was LED lamp (30 W), suitable for stimulating the growth of chlorophyll concentration (blue light 420÷480 nm, red light - 630÷690 nm), located in the length of 10 cm from the culture vessel. Separation process of the dried biomass from culture medium was consisted in: sedimentation, centrifugation (8000 rpm, 10 min), freezing with liquid nitrogen and lyophilisation (–80°C, < 10 Pa). The algal growth was assessed on the basis of the changes in optical density (absorption of visible light at 686 nm) and the concentrations of biogenic compounds. The culture was carried out until the phosphorus, which was a limiting factor for algal growth in suspended form, was exhausted. Within 46 days, the algae absorbed ∼ 12% of nitrogen by weight from the culture medium, and the final ammonium nitrogen concentration was ∼ 0.2 mg/dm³. The efficiency of biomass production was ∼ 3190 mg/dm³ (∼ 63 g/dm³ per day), while optical density ∼ 2.13. Based on the results, it was found that high efficiency of biomass production was influenced by light with a well-chosen wavelength spectrum, forced air circulation, limited evaporation of water from the vessel and sterile conditions during initiation of the culture. Further studies are needed to involve determining the concentration of different forms of nitrogen (nitrates, nitrites, ammonium nitrogen) in the culture medium and investigating the impact of process conditions on enhanced assimilation of biogenic compounds by algae, at following stages of their growth. A profound knowledge on these relationships and estimated costs will allow to continue experiments on increasing the productivity of algae biomass, which will be commonly recognized as economically reasonable.
Zbadano wydajność produkcji biomasy alg Chlorella sp., hodowanej na bazie pożywki syntetycznej BG-11, w zautomatyzowanym bioreaktorze zbiornikowym z mieszadłem mechanicznym w warunkach sztucznego naświetlania i napowietrzania. Kontrola hodowli polegała na spektrofotometrycznym pomiarze gęstości optycznej (OD) oraz badaniu zmian stężenia związków biogennych w podłożu hodowlanym, będących wynikiem zachodzących procesów metabolicznych. Hodowlę prowadzono przez 46 dni do momentu wyczerpania fosforu. Końcowa wydajność produkcji biomasy przekroczyła 3 g/dm³ przy jej średnim dobowym przyroście > 60 mg/dm³, a OD686 osiągnęła wartość 2,13. Na podstawie przeprowadzonych badań stwierdzono, że na wysoką efektywność produkcji biomasy alg kluczowy wpływ miały kontrolowane warunki hodowli, m.in. naświetlanie światłem o specyficznej długości fali (420÷480 nm, 630÷690 nm), wymuszony obieg powietrza, ograniczenie możliwości odparowania wody z naczynia hodowlanego oraz zapewnienie sterylnych warunków podczas inicjowania hodowli.
Źródło:: Inżynieria i Ochrona Środowiska; 2017, 20, 2; 237-248
1505-3695
2391-7253
Pojawia się w:: Inżynieria i Ochrona Środowiska
Dostawca treści:: Biblioteka Nauki

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