- Tytuł:
- Antitumoral effect of IL-12 gene transfected via liposomes into B16F0 cells
- Autorzy:
-
Speroni, Lucía
Gasparri, Julieta
de los A. Bustuoabad, Victoria
Chiaramoni, Nadia
Smagur, Andrzej
Szala, Stanisław
Taira, María
del V. Alonso, Silvia - Powiązania:
- https://bibliotekanauki.pl/articles/1040572.pdf
- Data publikacji:
- 2009
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
stearylamine
liposomes
B16F0 cells
B16 melanoma
IL-12 - Opis:
- Murine melanoma B16F0 cells were transfected with SA:DPPC:DOPE (2:1:1 molar ratio) liposomes associated with a plasmid encoding murine IL-12. Stearylamine, a cationic lipid, showed a greater transfection efficiency compared to DOTAP-containing liposomes. The lipid:DNA ratio was 2:1 (w/w). Control groups were mock transfected or transfected with an empty plasmid (pNeo). pNeo or IL-12 transfected cells and controls were inoculated intradermically into the dorsal region of the foot or the lateral flank of C57BL6 mice. Results showed that IL-12 expression had a marked effect on in vivo growth of B16 melanoma tumors developed in both anatomic sites, significantly retarding their growth and prolonging host survival.
- Źródło:
-
Acta Biochimica Polonica; 2009, 56, 2; 249-253
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł