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    Wyświetlanie 1-4 z 4
    Tytuł:
    Gametophyte and sporophyte of tree ferns in vitro culture
    Autorzy:
    Goller, K
    Rybczynski, J.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/57657.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    greenhouse collection
    gene bank
    botany
    multiplication
    gametophyte
    spore
    in vitro
    tree fern
    sporophyte
    Opis:
    Experiments had been carried out on gametophytes and very young fronds of sporophytes with application of Murashige and Skoog (1962) medium. The paper described the results of 15 years in vitro experiments on 16 species of tree ferns belonging to various genera: Blechnum, Cibotium, Cyathea and Dicksonia. Genus Cyathea was represented by: C. australis (R.Br.) Domin., C. capensis (L.f.) Sm., C. cooperi (F.Muell.) Domin, C. brownii Domin, C. dealbata (G.Forest) Sw., C. dregei Kunze, C. leichhardtiana (F.Muell.) Copel., C. robertsiana (F.Muell.) Domin., C. schanschin Mart., C. smithii Hook.f. and Cyathea sp. In case of genus Dicksonia only two species were introduced into our experiments: D. fibrosa Colenso and D. sellowiana Hook.. Taxa Blechnum was presented by B. brasiliense Desv. and Cibotium by C. glaucum (Sm.) Hook. and Arn. and C. schiedei Schltdl. and Cham.. The studied species presented various responses on culture conditions depending on the level of stage of development. Time required for spores germination differed between species and took from only a few to 16 weeks. Prothalium formations showed various types of growth presented by marginal meristems. For all investigated species long term gametophyte in vitro cultures was established. Mature gametophyte possessed functional antheridia and archegonia. Spontaneous fertilization helped to establish the culture of young sporophytes. For all species the ex vitro culture in greenhouse collection was established. Manipulation of sucrose content in the medium stimulated the multiplication of gametophytes, but its lack induced formation of gemmae. Apospory was observed when culture of very young fronds was extended for 6 months and new generation of gametophytes was developed. Finally, sporophytes of 12 species were obtained and they have been growing in our greenhouse.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2007, 76, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cytoembryology of infertile segregants of the hybrid Lupinus varius L. x L. digitatus Forsk
    Autorzy:
    Kazimierska, E M
    Kazimierski, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2048189.pdf
    Data publikacji:
    1995
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Lupinus digitatus
    cytoembryology
    embryology
    interspecific hybrid
    plant genetics
    gametophyte
    sterility
    vacuolation
    sporophyte
    megasporogenesis
    megagametogenesis
    chromosome
    Lupinus varius
    hybrid
    sporogenic cell
    Opis:
    In F₂, F₃ and F₄ generations of one hybrid line of Lupinus varius × L. digitatus, segregation into infertile and fertile plants in the ratio 3:1 was observed. Cytoembryological analyses showed that sterility was caused by irregularities in megasporocyte formation, in megasporogenesis and megagametogenesis. The following abnormalities lead to female sterility: no megasporogenic cells separated in nucelluses; in other nucelluses with megaspore mother cells, these cells underwent vacuolation and died before reduction division. In the megaspore mother cells, in which probably meiosis occurs, the megasporocyte division is irregular; a restitution nucleus is frequently formed after reduction division and such megasporocyte develops into an embryo sac. In 8-nucleate embryo sacs a change in the nucellus polarization was observed, while in rarely encountered embryo sacs the embryo cells underwent vacuolation and then died. Female sterility in the studied segregants is determined genetically, whereas sterilization of reproduction cells is a developing process throughout the period of sporocyte and female gametophyte formation.
    Źródło:
    Journal of Applied Genetics; 1995, 36, 3; 215-227
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A new and fast method to obtain in vitro cultures of Huperzia selago (Huperziaceae) sporophytes, a club moss which is a source of huperzine A
    Autorzy:
    Szypula, W.J.
    Mistrzak, P.
    Olszowska, O.
    Powiązania:
    https://bibliotekanauki.pl/articles/58228.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    clubmoss
    ground pine zob.clubmoss
    club moss zob.clubmoss
    in vitro culture
    Huperzia selago
    Lycopodium selago
    Huperziaceae
    sporophyte
    huperzine A
    sesquiterpene alkaloid
    pharmaceutical effect
    treatment
    Opis:
    This study presents a protocol for a fast and effective in vitro axenic culture of Huperzia selago (Huperziaceae Rothm.) sporophytes, a club moss which is a source of huperzine A, an alkaloid of a considerable therapeutic potential extensively investigated for its uses as treatment for some neurodegenerative diseases. The proposed procedure allowed approximately tenfold shortening of the species developmental stages with the omission of the gametophyte stage while the sporophyte mass could be increased tenfold within a 6-month period. The cultures were established using vegetative propagules (bulbils) procured from sporophytes growing in the wild without degrading the habitats of this endangered plant species. Explants underwent surface and internal disinfection to eliminate the epiphytic and endophytic bacteria and fungi. In in vitro cultures, the optimum results were achieved using Moore (Mr) medium without growth regulators or supplemented with 0.015 mg/l IBA and 0.3 mg/l kinetin. These media ensured both viability of the propagules and their further development. The biomass growth index for H. selago sporophytes grown from propagules, determined at 3 months of culture (1 passage) on Mr medium with IBA and kinetin was 650%. At 6 months, the biomass growth index increased to 1114%. Vigorous growth of adventitious roots, especially on Mr medium with the addition of 0.25 mg/l NAA, and callus formation on shoot apices were observed. At 6 months of culture, some sporophytes obtained from the bulbils were used as the initiating material for shoot subcultures, which developed best on Mr medium with IBA and kinetin.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2013, 82, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Equisetum telmateia Ehrh. morphotypes related to anthropogenic habitats
    Autorzy:
    Wrobel, D
    Powiązania:
    https://bibliotekanauki.pl/articles/58384.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    anthropogenic habitat
    phenotypic variation
    Polska
    biotope
    Alno-Padion
    Equisetum telmateia
    sporophyte
    growth regulator
    Calthion
    Europe
    morphotype
    distribution
    Giant Horsetail
    Opis:
    The Giant Horsetail (Equisetum telmateia) is the only representative of Equisetum genus included in the list of strictly protected species. In Central and Western Europe the species is found in communities belonging to alliances: Alno-Padion and Calthion. With progressing destruction of these biotopes, one can observe the phenomenon of this species moving to the habitats extremely anthropogenic in character. Frequent and intensive observations of this phenomenon were conducted in the Jasło - Krosno Dale area in southern Poland in three anthropogenic localities. In these localities three interesting, irregular Equisetum telmateia morphotypes were found: fo. serotinum subfo. proliferum, fo. spiralis and a morphotype with branched shoot. The phenomenon of morphological plasticity of sporophytes is thought to be connected with the action of genes, which regulate the identity of developing plant organs and their distribution. These genes perform a superior part in relation to the system of growth regulators.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2003, 72, 2
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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