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    Wyświetlanie 1-5 z 5
    Tytuł:
    Biodiversity of Chironomidae (Diptera) and genome response to trace metals in the environment
    Autorzy:
    Michailova, P.
    Szarek-Gwiazda, E.
    Kownacki, A.
    Warchalowska-Sliwa, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/55030.pdf
    Data publikacji:
    2011
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    biodiversity
    Chironomidae
    Diptera
    genome response
    trace metal
    aquatic environment
    somatic chromosome
    alteration
    insect
    aquatic insect
    metal concentration
    Opis:
    The effects of pollution on the biodiversity and genome response of Chironomidae larvae (Diptera, Insect) were evaluated in the trace metal contaminated water environments. No change on the Chironomid species diversity was found. The higher concentrations of trace metals (Cd, Pb, Cr, and Zn) affect the genome of 5 cytogenetically studied Chironomid species: Chironomus bernensis, C. plumosus, C. sp.1. Kiefferulus tendipediformis (cytotype 2), Glyptotendipes cauliginellus (syn. Glyptotendipes gripekoveni). Genome instability of Chironomid larvae was manifested by two ways: 1. Fixed chromosome rearrangements; homozygous inversions and tandem fusions created new gene linkage groups and show an intensive microevolutionary process of species. 2. Somatic structural (inversions, deficiencies, deletions, breaks); and functional alterations (decreasing the activity of the key structures: Balbiani rings (BRs) and Nucleolar Organizer (NOR)) in salivary gland chromosomes of cytogenetically studied Chironomidae species. Detecting somatic rearrangements in salivary gland chromosomes of these widely distributed aquatic insects is potentially one of the best validated bioassay and can be used as a cost effective early warning signals of environmental damage in freshwater basins.
    Źródło:
    Pestycydy; 2011, 1-4
    0208-8703
    Pojawia się w:
    Pestycydy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber [Cucumis sativus L.]
    Autorzy:
    Plader, W
    Hoshi, Y.
    Malepszy, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044260.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    heterochromatin
    4',6-diamidino-2-phenylindole
    euchromatin segment
    hybridization
    chromosome identification
    mitotic chromosome
    prometaphase chromosome
    somatic cell
    cucumber
    DNA
    chromomycin A3
    Cucumis sativus
    Opis:
    Each of the seven chromosomes in cucumber (Cucumis sativus L.) was identified using sequential staining with Chromomycin A₃ (CMA) and 4-6-diamidino-2-phenylindole (DAPI) as DNA base-specific fluorescent dyes. The present method using enzymatic digestion produced a high level of well-spread early-metaphase chromosome complements. After CMA and DAPI staining, reproducible fluorescence bands were obtained in mitotic prometaphase chromosomes. The CMA staining method made it possible to characterize whole chromosomes from prometaphase to mid-metaphase. Chromosome 1 had the largest and widest CMA-positive (CMA⁺) band from the proximal region to the interstitial region on the long arm in prometaphase. A large gap separating of the short arm from the long arm was always observed in chromosome 2 during prometaphase. The banding pattern of the short arm was similar to that of the long arm in chromosome 2. Chromosomes 1 and 2 in early metaphase had sharp and large CMA-positive and DAPI-negative (CMA⁺DAPI⁻) bands at the pericentromeric regions. In early metaphase, chromosome 3 was characterized by having a narrow CMA⁺DAPI⁻ band on the pericentromeric region of the short arm. Chromosomes 4 and 5 showed similar chromosome length and had a large CMA⁺ band at the distal region of the long arm. Chromosome 4 did not show any clear band in the short arm, while chromosome 5 showed a telomeric CMA⁺ band at the short arm and a clear CMA⁺DAPI⁻ band at the pericentromeric region. Chromosome 6 had a CMA⁺ band at the distal region and a weak CMA⁺ band at the proximal region in each of the arms. Chromosome 7 had an evident CMA⁺ band in the long arm and a CMA⁺ DAPI⁻ band in the pericentromeric region.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 3; 249-258
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Is the recently discovered EDA gene associated with anhidrotic ectodermal dysplasia?
    Autorzy:
    Kobielak, K
    Kobielak, A
    Trzeciak, W H
    Powiązania:
    https://bibliotekanauki.pl/articles/2046683.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ectodermal-mesodermal interaction
    X chromosome
    submicroscopic deletion
    hyperthermia
    Christ-Siemens-Touraine syndrome
    hybridization
    ectodermal dysplasia
    mutation
    somatic cell
    linkage analysis
    embryonic life
    transmembrane receptor
    Opis:
    The evidence from literature strongly suggests that Christ-Siemens-Touraine (CST) syndrome is associated with mutations of the newly discovered EDA gene. The gene is situated on the long arm of the X chromosome (Xq12.2-q13.1) and contains two exons separated by a 200 kbp intron. The 5’-untranslated region and most of the coding sequence are localized in exon 1, while three C-terminal amino acids are encoded by exon 2. The coding sequence was interrupted by translocations in three affected females: t(X;l), t(X;12), t(X;9), and submicroscopic deletions of the EDA gene were found in five males with CST syndrome, and point mutations were discovered in exon 1 in nine other patients. Northern blot analysis and in situ hybridization studies revealed that the EDA gene was expressed in the foetus, and postnatally in a specific type of skin cell and that the expression was limited to cells of ectodermal origin. A predicted protein product of the EDA gene contains 135 to 140 amino acids, organized in three distinct domains and may belong to class II transmembrane receptors.
    Źródło:
    Journal of Applied Genetics; 1997, 38, 3; 343-357
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cytogenetic markers of Brassica napus L. chromosomes
    Autorzy:
    Hasterok, R
    Maluszynska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2043142.pdf
    Data publikacji:
    2000
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    oil seed
    in situ
    silver staining
    chromosome number
    morphology
    molecular cytogenetics
    in vitro
    karyotype
    rDNA
    morphometric analysis
    plant breeding
    oilseed rape
    Brassica napus
    somatic hybridization
    transformation
    cytogenetic marker
    fluorescence
    hybridization
    genomic origin
    Źródło:
    Journal of Applied Genetics; 2000, 41, 1; 1-9
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Somaclonal selection of physiological mutants and several problems related to rice cell breeding
    Autorzy:
    Kinoshita, T
    Mori, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044460.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    chromosome variation
    japonica rice
    Oryza
    African rice
    physiological mutant
    gamma-radiation
    anther culture
    hybrid plant
    protoplast isolation
    callus induction
    callus formation
    aneuploidy
    indica rice
    herbicide tolerance
    hybrid
    Kitaake cultivar
    gametoclonal variation
    somatic hybrid
    rice
    Opis:
    Tolerance to a new herbicide, pyributycarb, was evaluated both at the plant and cellular levels. Several highly or moderately tolerant strains chosen at the plant level, showed a parallel relation of to tolerance at the cellular level. However, on the whole, correlation between total tolerance indices and survival rates of calli was not significant in 18 out of the 80 studied strains. As a result of somaclonal selection for two herbicides, lines NB-200 and NM-100 were regenerated from the tolerant calli screened with benthiocarb at 200 ppm, and molinate at 100 ppm, respectively. In the R₂ generation, both the lines displaned a stable tolerance both at the plant and cellular levels. Thus the highly tolerant mutant lines were developed from a moderately tolerant line, N-61, via in vitro selection. To achieve a short-cut method in the interspecific genetic exchange, a series of techniques related to cell fusion were established in rice and related species. Two kinds of somatic hybrids between the cultivar Kitaake and tetraploid Oryza species, O. punctata and O. officinalis, were successfully produced. Among the somatic hybrid plants, a wide range of chromosomal variation was observed. Aneuploid plants with a chromosome number around 2n = 72 (hexaploid), which are expected from a symmetric fusion between diploid and tetraploid strains, were obtained showing mixoploidy within a plant. Most of the somatic hybrids were characterized by intermediate features of plant-type showing high sterility, shattering of spikelets and reduced plant height. As an exception, a diploid plant, which was identified by RFLP analysis using the rDNA gene probe, closely resembled Kitaake and produced viable seeds. A tetraploid hybrid plant was also promising for the introduction of economically important characters through the reduction of chromosome numbers by doubled haploids. Gametoclonal variation and gamma radiation was applied to Kitaake. The mutation frequency was prominently increased by gamma ray treatment, especially at high doses of 200 Gy or 300 Gy. In the M₃R₂ or M₄R₃ generations, most of the variants showed unfavourable characters. Most of the mutant characters were governed by single or double recessive genes. Several mutants such as short culm and early flowering time might be used for rice breeding.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 1; 9-35
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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