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    Wyświetlanie 1-9 z 9
    Tytuł:
    Applications of Arthrospira platensis as an Alternative Source of Food, Maintaining Nutritional Security and Awareness Creation; there by Reducing Problems of Malnutrition in the Society
    Autorzy:
    Degnechew, Genene Demisu
    Buzayehu, Desisa Benti
    Powiązania:
    https://bibliotekanauki.pl/articles/1111949.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Antioxidant
    Arthrospira platensis
    Cyanobacteria
    Malnutrition
    Single cell protein
    Opis:
    An increase in population has led to explorations of alternative sources of food, nutrition and energy. This search and the maintenance of nutritional security are essential dimensions of sustainable communal growth and development. This study revises evidences for alternative food production from Arthrospira platensis. The use of Arthrospira platensis for food is increasingly relevant as its components (proteins, lipids, minerals and vitamins) have substantial potential to be competitive with the same components from other food sources, and Arthrospira platensis-based food production is relatively low in price and holds medicinal properties.
    Źródło:
    World News of Natural Sciences; 2018, 19; 1-8
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of the effects of bleomycin and ionizing radiation in two sublines of murine lymphoma L5178Y
    Autorzy:
    Kruszewski, M.
    Zaim, J.
    Grądzka, I.
    Szumiel, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/147561.pdf
    Data publikacji:
    2001
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    bleomycin
    comet assay
    DNA damage
    gamma radiation
    single cell gel electrophoresis
    Opis:
    We compared the effects of bleomycin (BLM) and ionizing radiation on two sublines of murine lymphoma L5178Y (LY): LY-R, radiation resistant and LY-S, radiation sensitive. This radiosensitivity difference is related to the ability to rejoin DNA double strand breaks. LY-S cells were about two times more sensitive to BLM than LY-R, similarly as in the case of sensitivity to X rays. Since there was no difference in the P-glycoprotein-related drug transport system between the sublines, it could be expected that the enhanced sensitivity of LY-S cells to BLM was caused by the DNA repair defect. Growth disturbances in BLM treated cell populations were proportional to the lethal effect and their duration was observed until elimination of dead cells (3-6 days after 50 ěM BLM, 1 h at 37oC). There was no slow growth phase accompanied by normal viability, as previously described for X-irradiated LY-S cells. Initial DNA damage, estimated with the single cell gel electrophoresis method was linearly related to BLM dose in LY-S cells; in LY-R cells - in the low dose range (up to 10 ěM) - there was more damage than in LY-S cells, however, at higher doses the dose - effect curves became identical. The doseeffect relationship for ă rays was linear and identical in both cell sublines. DNA damage distribution in BLM treated cells was much less uniform as compared to that in irradiated cells and indicated the presence of cells with severely damaged DNA, a feature typical for BLM action in vitro.
    Źródło:
    Nukleonika; 2001, 46, 3; 81-86
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of a CAPA-PVK [Ixori-PVK] from single cells of the gulf Gulf Coast tick, Amblyomma maculatum
    Autorzy:
    Neupert, S.
    Russell, W.K.
    Russell, D.H.
    Strey, O.F.
    Teel, P.D.
    Strey, A.
    Nachman, R.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/55404.pdf
    Data publikacji:
    2008
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    diuresis
    MALDI analysis
    single cell
    periviscerokinin
    Boophilus microplus
    Ixodidae
    immunocytochemistry
    Gulf Coast tick
    amino acid sequence
    MALDI-TOF mass spectrometry
    neurohormone
    Ixodes ricinus
    Amblyomma maculatum
    Opis:
    MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequence of a CAPA-PVK in the Gulf Coast tick, Amblyomma maculatum. Single cell analysis allowed the identification of the amino acid sequence of Ixori-PVK (PALIPFPRV-NH2), a periviscerokinin which had previously been identified from two other ticks, Ixodes ricinus and Boophilus microplus. The identification indicates greater conservation of sequence for the CAPA-PVK/CAP2b family in ticks as compared with insects. Side-chain fragmentation experiments provided data to distinguish between Leu/Ile ambiguities. The tick CAPA peptide shows a high sequence homology with other members of the insect periviscerokinin/ CAP2b peptides, which are associated with the regulation of critical physiological processes such as diuresis. Thus, the identification of this neuropeptide will provide the experimental basis to better understand regulation of water balance in these arthropods, providing a potential opportunity to develop neuropeptide-based control strategies against these livestock pests.
    Źródło:
    Pestycydy; 2008, 1-2; 67-73
    0208-8703
    Pojawia się w:
    Pestycydy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Crashworthiness and comparative analysis of polygonal single and bi-tubular structures under axial loading – experiments and FE modelling
    Autorzy:
    Kannan, I. Vimal
    Rajkumar, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/1839693.pdf
    Data publikacji:
    2021
    Wydawca:
    Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
    Tematy:
    crashworthiness
    thin-walled structures
    single and multi-cell tubular structures
    energy absorption
    key performance indicators
    Opis:
    This article aims to present a report of experimental and numerical investigations on crashworthiness characteristics of single and multi-cell/bi-tubular structures. Novel multi--cell/bi-tubular structures are proposed in order to improve the crashworthiness performance, LS-DYNA FE software is applied for the modelling of axial crashing behaviour to validate with experimental results and a good agreement is observed. The KPIs are used to compare various structures and to determine the best performing ones. The investigations reveal that the HMC4 has significantly obvious effects on the structural crashworthiness and improved 515% energy absorption efficiency. Afterward, a parametric study has been carried out for the best energy absorber.
    Źródło:
    Journal of Theoretical and Applied Mechanics; 2021, 59, 1; 81-94
    1429-2955
    Pojawia się w:
    Journal of Theoretical and Applied Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Grafowa metoda segmentacji obiektów w postaci skupisk na przykładzie obrazów kometowych
    Graph-based aggregate object image segmentation method on example of comet assay images
    Autorzy:
    Bal, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/154889.pdf
    Data publikacji:
    2012
    Wydawca:
    Stowarzyszenie Inżynierów i Techników Mechaników Polskich
    Tematy:
    analiza obrazów
    obiekty w postaci skupisk
    minimalne drzewo rozpinające
    test kometowy
    jednokomórkowa elektroforeza żelowa
    image analysis
    image segmentation
    aggregate objects
    minimum spanning tree
    comet assay
    single cell gel electrophoresis
    Opis:
    W pracy przedstawiono propozycję metody segmentacji obiektów będących skupiskami - przykładem takich obiektów są tzw. komety, które są wynikiem jednokomórkowej elektroforezy żelowej. Opracowana metoda działa dwuetapowo: etap 1. to segmentacja służąca wyznaczeniu elementów składowych należących do obiektów, etap 2 wykorzystuje minimalne drzewo rozpinające do określenia zbioru elementów tworzących poszczególne obiekty, obszar poszczególnych obiektów wyznaczany jest jako otoczka wypukła odpowiedniego drzewa rozpinającego.
    This paper deals with a problem of segmentation of aggregate objects, that is objects which are formed by a set of unconnected elements smaller than the object itself. Images of such a type of objects are very difficult for segmentation. An example of this type of objects are "comets" (Fig. 1, left column) from Single Cell Gel Electrophoresis images (also called comet assay images). In comet assay images the comet region is formed by unconnected fragments of DNA. Because of not satisfying results of comet segmentation with use of the standard methods, a new method for segmentation of such images was developed. The new method works in two stages. The first stage is the image segmentation-for comets the Bernsen binarization method (Eqs. (1) and (2)) with median filtering of the obtained results was chosen-the result of this stage is a set of comet elements ei which represent DNA fragments (Fig. 1, the 2nd column). In the second stage the minimum spanning trees Tp are created (Fig. 1, 3th column)-graph vertexes vi represent elements ei, and length dij of edge eij between vertexes vi and vj is equal to the closest distance between pixels of elements ei and ej-then for each connected tree Tp its convex hull which defines the region of comet Kp (Fig. 1, the 4th column) is created. In case of defects appearing in comet images, the incorrect region can be rejected e.g. by use of geometrical or photometrical features of the regions.
    Źródło:
    Pomiary Automatyka Kontrola; 2012, R. 58, nr 4, 4; 313-315
    0032-4140
    Pojawia się w:
    Pomiary Automatyka Kontrola
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Defining quality work of a pneumatic seeding device with an extra disk
    Autorzy:
    Abramova, V.
    Vasylkovskyi, O.
    Vasylkovska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/411329.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Oddział w Lublinie PAN
    Tematy:
    seeding device
    single seed sowing
    cell
    seeds
    extra disk
    quality
    Opis:
    This article is devoted to solving the problem of single-seed sugar beet sowing with a pneumatic seeding device. As a result of experimental researches the influence of sowing device parameters equipped with an additional disk for quality sowing were defined.
    Źródło:
    ECONTECHMOD : An International Quarterly Journal on Economics of Technology and Modelling Processes; 2016, 5, 3; 191-196
    2084-5715
    Pojawia się w:
    ECONTECHMOD : An International Quarterly Journal on Economics of Technology and Modelling Processes
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Thapsigargin inhibits the effects of noradrenaline and high [Ca2]0 on kinetics but not on amplitude of contraction in the single myocytes of guinea pig heart
    Autorzy:
    Janiak, R
    Lewartowski, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/69734.pdf
    Data publikacji:
    1995
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    cell isolation
    sarcoplasmic reticulum
    cardiac myocyte
    human physiology
    noradrenaline
    guinea pig
    single myocyte
    heart
    thapsigargin
    Źródło:
    Journal of Physiology and Pharmacology; 1995, 46, 1
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Association between polymorphisms in CHRNA3 and PHACTR2 gene and environment and NSCLC risk in Chinese population
    Autorzy:
    Lou, Guangyuan
    Zhang, Yongjun
    Bao, Wenlong
    Deng, Dehou
    Powiązania:
    https://bibliotekanauki.pl/articles/1039210.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Non-small cell lung cancer
    phosphatase and actin regulator 2 (PHACTR2)
    cholinergic receptor
    nicotinic
    alpha 3 (CHRNA3)
    single-nucleotide polymorphism
    Opis:
    Aims. This study aimed to investigate CHRNA3 (rs8040868) and PHACTR2 (rs9390123) single-nucleotide polymorphisms (SNPs) for association with non-small-cell lung cancer (NSCLC) risk in a Chinese population, and whether the environment affects the genetic polymorphisms. Methods. This case and control study included 500 NSCLC patients and 500 age-matched healthy controls. CHRNA3 (rs8040868) and PHACTR2 (rs9390123) SNPs were genotyped and associated for NSCLC risk by computing the odds ratio and 95% confidence interval from multivariate unconditional logistic regression analyses with adjustment of age. Results. The minor allele frequency (MAF) of CHRNA3 (rs8040868) and PHACTR2 (rs9390123) was 0.350 (C) and 0.397 (C), respectively. The frequencies of genotype and allele in CHRNA3 (rs8040868) and PHACTR2 (rs9390123) were not significantly different between the cases and controls, or between either of the subgroups. Conclusion. Although rs8040868 and rs9390123 SNPs are not associated with NSCLC risk in Chinese population, the results strongly suggest that geographical agents interact with human genetic polymorphism independent of ethnic background.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 4; 765-768
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation
    Autorzy:
    Pilarek, M.
    Godlewska, K.
    Kuźmińska, A.
    Wojasiński, M.
    Dąbkowska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/184988.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    wave-induced agitation
    small-scale animal cell culture
    CP5 chondrocytes
    fibrousbased
    scaffold
    single-use bioreactor
    wzbudzanie falowe
    hodowla komórek zwierzęcych
    Chondrocyty CP5
    szafot
    bioreaktor
    Opis:
    One of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to inefficient diffusion of culture medium components observed in such non-mixed culture systems, especially in the case of cell-inoculated fiber-based dense 3D scaffolds, inside which the intensification of mass transfer is particularly important. The applicability of a prototyped, small-scale, continuously wave-induced agitated system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) non-agitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the volumetric glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of the presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. Aside from the practicability of the prototyped system, we believe that it could also be applied as a standard method offering advantages for all types of the daily routine laboratory-scale animal cell cultures utilizing various fiber-based biomaterials, with the use of only regular laboratory devices.
    Źródło:
    Chemical and Process Engineering; 2017, 38, 2; 321-330
    0208-6425
    2300-1925
    Pojawia się w:
    Chemical and Process Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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