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Tytuł:
Toxoplasma gondii and women of reproductive age: an analysis of data from the Chair of Microbiology, Jagiellonian University Medical College in Cracow
Autorzy:
Salamon, D.
Bulanda, M.
Powiązania:
https://bibliotekanauki.pl/articles/6209.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Toxoplasma gondii
woman
toxoplasmosis
zoonosis
risk factor
seroprevalence
reproductive age
Chair of Microbiology
Jagiellonian University
Medical College
Polska
Opis:
The aim of the study is to determine the seroprevalence and risk factors for Toxoplasma gondii infection in women of reproductive age within the Małopolska province who were examined in the Laboratory of Microbiological Diagnostics, Chair of Microbiology in Cracow in 2013–2014. Seventy-eight questionnaires completed by women of reproductive age (mean age 29.91±6.56) were analysed. In total, 82% of respondents signed up for serological testing while already pregnant (33 patients in the age group 18–29 years and 30 patients in the age group 30–39 years). Twentyseven percent had no previous contact with the parasite. A statistically significant (chi-square=7.722, p=0.005) correlation between permanent residence in the countryside and the presence of anti-T. gondii antibodies was found. A significant correlation was shown in the studied group between a lack of contact with soil and negative results of serological tests for toxoplasmosis (chi-square=4.116, p=0.042). The majority of the surveyed women had already encountered this parasite. It seems that special attention should be given to rural women. Implementation of more precise testing in the diagnosis of toxoplasmosis is also essential.
Źródło:
Annals of Parasitology; 2014, 60, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of Toxoplasma gondii in wild boars [Sus scrofa] in the Slovak Republic
Autorzy:
Antolova, D
Reiterova, K.
Dubinsky, P.
Powiązania:
https://bibliotekanauki.pl/articles/49413.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
parasite
toxoplasmosis
wild boar
Toxoplasma gondii
Sus scrofa
Slovak Republic
seroprevalence
protozoan parasite
Opis:
Toxoplasma gondii is a protozoan parasite of great medical and veterinary importance. The aim of this study was to determine the seroprevalence of toxoplasmosis in wild boars hunted in the Slovak Republic in 2003. Examination of 320 wild boars revealed a seroprevalence of 8.1%. The majority of seropositive wild boars came from the north-western and southern regions of Slovakia. This study indicates that T. gondii infection is common in wild boars in the Slovak Republic, underlines its zoonotic potential and the importance of high standards of hygiene during the handling of game.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of Q fever, brucellosis and leptospirosis in farmers and agricultural workers in Bari, Southern Italy
Autorzy:
Monno, R
Fumarola, L.
Trerotoli, P.
Cavone, D.
Giannelli, G.
Rizzo, C.
Ciceroni, L.
Musti, M.
Powiązania:
https://bibliotekanauki.pl/articles/49842.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
zoonosis
farmer
agricultural worker
Bari
Italy
Q fever
seroprevalence
brucellosis
leptospirosis
exposition
Coxiella burnetii
Brucella
Leptospira
animal breeding
worker
human disease
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 205-209
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of Neospora caninum in free living and farmed red deer [Cervus elaphus] in Poland
Autorzy:
Gozdzik, K
Jakubek, E.B.
Bjorkman, C.
Bien, J.
Moskwa, B.
Cabaj, W.
Powiązania:
https://bibliotekanauki.pl/articles/32072.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Polska
free living red deer
farmed red deer
red deer
Cervus elaphus
seroprevalence
Neospora caninum
ELISA test
immunoblot
animal disease
animal infection
Opis:
Serum samples from 47 free living and 106 farmed red deer (Cervus elaphus) from the Mazurian Lake District in north-east Poland were investigated for the presence of antibodies to Neospora caninum. A modified Neospora iscom-ELISA was used for initial screening. All sera with optical density (OD) values exceeding 0.400 absorbance units were further investigated by Western blot analysis. Eighteen sera were positive in both tests. Six of these were from free living and 12 from farmed animals giving prevalence of 13 and 11%, respectively. This is the first report of N. caninum infection in farmed and free-living red deer living in the same region where neosporosis was confirmed in cattle and the first evidence of exposure to the parasite in red deer in Poland.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 117-120
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of Bordetella pertussis antibodies among a group of young adults
Autorzy:
Skiba, A.
Raman, S.
Rzucidlo, I.
Sereda, D.
Koziol, M.M.
Powiązania:
https://bibliotekanauki.pl/articles/2098551.pdf
Data publikacji:
2021
Wydawca:
Instytut Medycyny Wsi
Tematy:
seroprevalence
Bordetella pertussis
antibody
pertussis
respiratory system
adolescent
young adult
Opis:
Introduction. Bordetella pertussis is a Gram-negative rod which attacks the respiratory system and causes whooping cough disease. Despite the high percentage of childhood vaccination amongst the population an increasing number of pertussis cases have been observed over time, especially among adolescents and young adults. Objective. The aim of the study was to analyze the concentration of IgG anti-PT Bordetella antibodies in the serum of young adults in the aspect of maintaining immunity. Materials and method. The study group consisted of 30 patients, medical students aged 21–26 years. They were interviewed with a questionnaire, which asked about their general health, medications taken, basic information about whooping cough, or vaccination status against B. pertussis. The anti-PT Bordetella levels were measured in the collected serum by a commercial ELISA kit (enzyme-linked immunosorbent assay). Results. All patients had previously received a mandatory vaccination for pertussis as a child, and 36.6%had received a booster dose after the age of 18. The total average antibody level was 13.977 IU/mL (range: <5.00 – 50.74) and 40% of samples had a very low concentration (<5 IU/mL). Conclusions. Current findings suggest that post-vaccine immunity decreases over time, while infection susceptibility among younger age groups who have not yet been fully immunized has increased. Methods that could prevent and enable risk stratification of whooping cough include the screening test for anti-PT Bordetella antibody, the improvement of diagnostics, and an additional booster dose.
Źródło:
Journal of Pre-Clinical and Clinical Research; 2021, 15, 3; 126-128
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of BHV-1 (bovine herpesvirus type 1) among non-vaccinated dairy cattle herds with respiratory disorders
Autorzy:
Rypula, K.
Ploneczka-Janeczko, K.
Kita, J.
Kumala, A.
Zmudzinski, J.F.
Powiązania:
https://bibliotekanauki.pl/articles/30913.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
seroprevalence
bovine herpesvirus type 1
non-vaccinated animal
dairy cattle
herd
respiratory disorder
ELISA test
seroconversion
Opis:
The objective of this study was to estimate a herd-level seroprevalence of bovine herpesvirus type 1 (BHV-1) in herds with clinical symptoms of the respiratory tract. Eighty-three herds with suspected BHV-1 infection were selected and divided into two categories with respect to their size: small (n=27) and large herds (n=56). Samples were collected from calves, heifers and cows older than 24 months. Seroprevalence was determined using the gB ELISA test. The herd level seroprevalence was estimated as 53% (44/83) in the tested herds, 11.1% (3/27) in the small herds and 73.2% (41/56) in the large herds. Our study suggests that the current biosecurity measures still warrant improvement.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of antibodies to classical swine fever virus and porcine reproductive and respiratory syndrome virus in healthy pigs in Hunan Province, China
Autorzy:
Yu, H.
Zhang, L.
Cai, Y.
Hao, Z.
Luo, Z.
Peng, T.
Liu, L.
Wang, N.
Wang, G.
Deng, Z.
Zhan, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16539345.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
classical swine fever virus (CSFV)
porcine reproductive and respiratory syndrome virus (PRRSV)
pig
seroprevalence
antibody
Opis:
Classical swine fever (CSF) and porcine reproductive and respiratory syndrome (PRRS) are responsible for major economic losses and represent a threat to the swine industry worldwide. Routine surveillance serology for CSF and PRRS viruses is critical to maintaining the health status of sow farms in Hunan Province, which is one of the top pig production provinces in China. The aim of our study was to investigate the serological statistics of CSF virus (CSFV) and PRRS virus (PRRSV) in Hunan Province. The cohort serum samples were collected from vaccinated and unvaccinated pigs. Our findings showed that the average rates of CSFV and PRRSV antibody seropositivity were 82.2% (95% CI: 80.1-84.3) and 84.8% (95% CI: 82.5-87.1), respectively, in the immunized group and that these rates were higher than those in the unvaccinated group (58.6% for CSFV and 47.8% for PRRSV). Additionally, the level of CSFV antibody in piglet serum declined gradually with age, whereas PRRSV-specific antibody level increased initially (1 to 2 weeks old) and then declined with age (2 to 4 weeks old). In summary, we investigated the difference in CSFV/PRRSV antibody levels among piglets at various weeks old (1 to 4 weeks) to further establish the duration of maternal immunity in piglets. In addition, routine monitoring of CSFV/PRRSV antibodies in immunized pigs was carried out to evaluate the efficacy of vaccination.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 375-381
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of anti-HAV total antibodies among workers in wastewater treatment plants
Autorzy:
Toseva, Elka I.
Atanasova, Mariya V.
Turnovska, Tanya H.
Powiązania:
https://bibliotekanauki.pl/articles/2159910.pdf
Data publikacji:
2017-12-21
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
anti-HAV total antibodies
immunization
Hepatitis A
seroprevalence
wastewater
workers
Opis:
Objectives Data on high frequency of hepatitis A virus (HAV) antibodies for wastewater treatment staff is contradictory. Literature lacks data on the seroprevalence of antibodies to HAV (anti-HAV) among workers in wastewater treatment plants (WWTPs) in Bulgaria. The aim of this study is to establish a specific humoral immune response to hepatitis A virus – anti-HAV total antibodies among staff in WWTPs. Material and Methods A complex study of health and working conditions included 110 subjects working in 3 WWTPs in Bulgaria (74% of all workers in the 3 studied WWTPs and 20% of all employees in Bulgaria registered in 2014 under the wastewater collection, discharge and treatment code of economic activity). Workers had been differentiated in 3 groups on the basis of their occupational work: operators, support staff and other workers exposed to biological agents. Venous blood from all 110 subjects was tested once for carriers of HAV antibodies. Results Anti-HAV total antibodies were found for 52.7% of workers in WWTPs. There is a positive association between activity performed in WWTPs (operators, maintenance personnel and others exposed) and a positive one for the presence of anti-HAV (Chi2 = 6.882, df = 2, p = 0.032). Odds ratio (OR) for hepatitis A increases 2.9 times in the group of operators vs. others exposed to biological agents in WWTPs (OR = 2.914, 95% confidence interval (CI): 1.149–7.393, Fisher’s p = 0.039). Odds ratio for hepatitis A increases 4.3 times in the group of support staff from WWTPs vs. others exposed to biological agents in WWTP (OR = 4.295, 95% CI: 1.075–17.167, Fisher’s p = 0.049). Conclusions Higher frequency of anti-HAV antibodies among operators and maintenance personnel at WWTPs has been established as compared to other workers exposed to biological agents in WWTPs. There is a positive association between increasing age of the workers and the presence of anti-HAV. Int J Occup Med Environ Health 2018;31(3):307–315
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2018, 31, 3; 307-315
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence and occupational risk survey for Coxiella burnetii among exposed workers in Sicily, Southern Italy
Autorzy:
Fenga, Concettina
Gangemi, Silvia
De Luca, Annamaria
Calimeri, Sebastiano
Lo Giudice, Daniela
Pugliese, Michela
Licitra, Francesca
Alibrandi, Angela
Costa, Chiara
Powiązania:
https://bibliotekanauki.pl/articles/2177166.pdf
Data publikacji:
2015-07-07
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
seroprevalence
Coxiella burnetii
occupational hazard
anthropozoonosis
health surveillance
prevention
Opis:
Objectives The aim of this survey was to assess the seroprevalence of antibodies against Coxiella burnetii (C. burnetii) in subjects at risk of exposure in Sicily, Southern Italy. Material and Methods Prevalence of IgG antibodies to C. burnetii phase II antigens was evaluated by ELISA in a group of 140 workers at risk of exposure (38 veterinarians, 38 slaughterhouse workers, 44 livestock handlers, 20 laboratory and technical personnel) included in a medical surveillance program and in 42 control subjects. Positive samples were classified as suggestive of prior exposure to C. burnetii. Results Antibodies against C. burnetii were detected in 88 out of 140 (62.9%) exposed workers and in 6 out of 42 (14.3%) subjects of the control group. The variables evaluated did not seem to have a significant effect on seropositivity to Coxiella with the exception of symptoms in the last 6 months preceding the survey. Conclusions Our study demonstrated a high seroprevalence of C. burnetii in the group of exposed workers in comparison to non-exposed subjects of the control group. Clinical illness appears to be rare; nevertheless, physicians should consider Q fever in patients with compatible symptoms and occupational exposure to animals and their products. As aerosols represent the main route of infection in animals and humans, these workers are strongly advised to wear respiratory masks. In addition, occupational physicians should consider routine serologic evaluation and vaccination of occupationally exposed workers.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2015, 28, 5; 901-907
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Serological evidence of lack of contact with caprine herpesvirus type 1 and bluetongue virus in goat population in Poland
Autorzy:
Czopowicz, M.
Kaba, J.
Szalus-Jordanow, O.
Nowicki, M.
Witkowski, L.
Frymus, T.
Powiązania:
https://bibliotekanauki.pl/articles/30762.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
serological evidence
caprine herpesvirus type 1
bluetongue virus
goat
population
Polska
seroprevalence
small ruminant
animal infection
Opis:
Investigation into herd-level seroprevalence of caprine herpesvirus type 1 (CpHV-1) and bluetongue virus (BTV) was conducted in 2007 in Poland. It involved the entire population of goats covered by a milk recording program in 2007, which included 49 goat herds. The number of goats examined in each herd was determined statistically in order to detect the presence of at least one seropositive animal in a herd with a 95% probability and simple random method of sampling was applied. No antibodies to CpHV-1 or BTV were detected. Further calculations were carried out to determine the herd-level true seroprevalence, taking into account sensitivity and specificity of the test as well as several other factors. It can be concluded that till the middle of 2007 population of Polish goats covered by the milk recording program remained negative with respect to CpHV-1 and BTV.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Serological cross-sectional survey of equine infectious anemia in Saudi Arabia
Autorzy:
Kasem, S.
Hashim, O.
Alkarar, A.
Hodhod, A.
Elias, A.
Abdallah, M.
Al-Sahaf, A.
Al-Doweriej, A.
Qasim, I.
Abdel-Moneim, A.S.
Powiązania:
https://bibliotekanauki.pl/articles/16539306.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
equine infectious anaemia
equine
ELISA
Saudi Arabia
seroprevalence
Opis:
The equine infectious anaemia virus (EIAV) is one of the most serious equine diseases worldwide. There is scarce information on the epizootiology of equine infectious anaemia (EIA) in Saudi Arabia. Given the importance of the equine industry in Saudi Arabia, this cross- -sectional study aims to provide information about the prevalence of EIAV based on serological surveillance of the equine population in the country. A total of 4728 sera samples were collected (4523 horses and 205 donkeys) between December 2017 and November 2019. All samples were tested using commercially available EIAV ELISA. All tested samples showed negative results for EIAV antibodies with a 95% confidence interval. The results provided evidence that Saudi Arabia’s equine populations (horses and donkeys) are currently free of EIAV. The results also suggest the need for continuous monitoring of EIAV and strict regulation when importing horses from other countries.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 365-368
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroepidemiological study of canine Leishmania infantum and Toxoplasma gondii infections in Shanghai, China, and analysis of risk factors
Autorzy:
Jiang, Wei
Wang, Yan
Liu, Yingchun
Li, Tao
Chen, Yongjun
Wang, Shaohui
Han, Xiangan
Wang, Quan
Powiązania:
https://bibliotekanauki.pl/articles/990968.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
seroprevalence
leishmania infantum
toxoplasma gondii
household dogs
risk factors
Opis:
The aim of this study was to determine the seroprevalence of Leishmania infantum and Toxoplasma gondii among household dogs in Shanghai (the most important industrial and commercial city in China), and to assess the possible risk factors associated with the infection. During 2014–2015, a total of 408 sera were collected from healthy household dogs and tested for L. infantum and T. gondii infection using commercial ELISA kits. The endemic characteristics according to gender, age group and breed were revealed by statistical descriptions and inference. The positive rates of L. infantum infection (24/408, 5.9%) were lower than those of T. gondii infection (37/408, 9.1%), and co-infection with both parasites was detected in seven dogs (7/408, 1.7%). Seropositivity for either parasite was more likely associated with age: the seroprevalence of T. gondii infection ranged from 1.3% (dogs≤1 year) – 18.7% (dogs>6 years), whereas that of L. infantum ranged from 1.3% (dogs≤1 year) – 9.9 % (dogs>6 years). Interestingly, the rates of exposure to both L. infantum and T. gondii were higher in males than in females. Relatively higher exposure rates for L. infantum and T. gondii were also observed in crossbred dogs compared with purebred dogs. However, neither gender nor breed is likely a determining factor for infection with these two parasites (P > 0.05). Identification of the risk factors that underlie these differences may help in the prevention of L. infantum and T. gondii infection in household dogs. To the best of our knowledge, this is the first report of L. infantum and T. gondii infection in household dogs in Shanghai, which shows that these two important parasites are still prevalent in this region. Therefore, it is necessary to take integrated strategies for prevention and control of infection in animals, which could help to reduce human infection in the region.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Serodiagnosis of Toxoplasmosis using Lateral Flow Chromatographic Immunoassay among Animals and Humans in Sunsari District of Nepal
Autorzy:
Sah, Ramesh Prasad
Talukder, Md Hasanuzzaman
Rahman, A. K. M. Anisur
Powiązania:
https://bibliotekanauki.pl/articles/1167051.pdf
Data publikacji:
2018
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Animals
Nepal
human
seroprevalence
toxoplasmosis
Opis:
Toxoplasmosis is a cosmopolitan parasitic zoonosis, infecting human and other warm blooded animals worldwide. This disease has economic importance in regard to animal reproduction, and it leads to abortions and neonatal complications in humans. This study was carried out to determine the seroprevalence of Toxoplasma gondii in sheep, cattle, cats and human in Inaruwa and surrounding areas of Sunsari district, Nepal. Altogether 336 blood samples, of which 50 from sheep, 92 from cattle, 44 from cats and 150 from human were collected and tested immediately using lateral flow chromatographic immunoassay (Toxo IgG/IgM Combo Rapid test®). Associated biometric information such as age, sex, pregnancy status, occupation, association with cat was recorded and analyzed to determine the association of risk factors with the disease. Data were analyzed using R 3.2.2 (The R foundation for Statistical Computing, 2015). Seroprevalence of toxoplasmosis was detected 12.00% (95% CI: 4.53- 24.31%) in sheep, 8.70% (95% CI: 3.83- 16.42%) in cattle, 36.36% (95%CI: 22.41- 52.23%) in cats and 12.67% (95% CI: 7.80- 19.07%) in human. In case of human, 31 to 45 years age group were found more susceptible to toxoplasmosis (21.74%, OR: 6.4) in comparison to 21 to 30 years (10.0%) and up to 20 years (4.17%) age groups. Toxoplasmosis was found highly significantly associated with abortion (58.33%, OR= 15.4, P=0.0001) in human in the tested individuals. Regarding occupation, 20.83% butchers were seropositive followed by farmers (15.52%), housewives (10.0%) and diagnostic lab technicians (8.0%). Female and higher age group showed high prevalence of toxoplasmosis in all studied species. Therefore, this assay is a useful method for the serological screening of toxoplasmosis in different animals and humans.
Źródło:
World Scientific News; 2018, 105; 145-156
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of Toxoplasma gondii infection in cats in southwestern Poland
Autorzy:
Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Zając, V.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2081746.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
PCR
seroprevalence
Toxoplasma gondii
IFAT
cats
oocysts
coproscopy
Opis:
Objective. An assessment of the prevalence of Toxoplasma gondii infection in cats from southwestern Poland using serology, coproscopy and PCR methods. Materials and method. In total, 208 cats (139 females and 68 males), aged 0.5–12 years (mean=2.6) from 25 localities in southwestern Poland were examined by indirect immunofluorescence assay (IFAT) to estimate the T. gondii serological status. Faecal samples of 41 cats were examined for the presence of oocysts/DNA T. gondii by microscopy and Real-time/nested PCR. After flotation (with NaNO3), pellets from faecal samples were disrupted by 10 cycles of freezing (liquid nitrogen) and warming. DNA was extracted using QIamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instruction. Results. The positive results in IFAT for anti-T. gondii IgG and IgM antibodies were found in 143 of 208 tested cats (68.8%). Among positive results, 14.5%, 34.1% and 51.4% were detected in titre ranges 128–512, 1,000–2,000 and ≥ 4,000, respectively. In 23.1% of cat sera anti- T. gondii IgM antibodies were found. The prevalence of anti-Toxoplasma antibodies was significantly greater in older cats (>1 year) (83.5%) than in younger cats (48.3%) (P<0.05), in females (74.1%) than in males (58.8%) (P<0.05), and in cats kept outdoors than indoors (69.7% vs. 16.7%) (P<0.01). Among the 41 faecal samples examined, the presence of structures resembling T. gondii oocysts was found in 2 samples (4.9%), and for one of these samples (2.4% of the total) the result was also confirmed by PCR. Conclusions. T. gondii infection in domestic cats is highly prevalent in southwestern Poland. Information on the prevalence of infection in cats can be useful for assessing T. gondii environmental contamination and the risk for public health.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 3; 1-5
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł

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