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    Wyświetlanie 1-7 z 7
    Tytuł:
    Serological investigation in children infected with Ascaris lumbricoides
    SEROLOGICZNE BADANIA U DZIECI ZARAŻONYCH ASCARIS LUMBRICOIDES
    Autorzy:
    Małafiej, E.
    Śpiewak, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148469.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    infection
    serological diagnostics
    ELISA test
    Ascaris lumbricoides
    child
    protein antigen
    serological investigation
    Opis:
    The study included 260 hospitalised children with suspected infection with human ascaris. In serological diagnostics a protein antigen obtained from Ascaris lumbricoides was used. ELISA method was applied. IgG antibodies were detected. Positive results were found in 15% of the examined children. No relation to the gender or demographic conditions was found. The most frequently observed symptoms in the patients with Ascaris lumbricoides were: abdominal pain - 87%. diarrhoea 15%. In 31% of the cases eosinophilia was found. Scatoscopy was carried out for all the patients, using the PARASEPT system and Kato and Miura methods as well as decantation and tlotation. The examination. which was repeated three times, did not show cysts or eggs. Serological investigation exhibits higher sensitivity than the traditional methods. Their use in recognising ascariasis in humans significantly facilitates diagnostic procedures, especially in the lung phase of the disease, the larval stage or in cases of infection with an individual parasite, when the faeces samples do not contain the eggs. Serological investigation is also useful in all cases of suspected VLM.
    Źródło:
    Wiadomości Parazytologiczne; 2001, 47, 4; 585-590
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serological investigation in children infected with Ascaris lumbricoides
    Autorzy:
    Malafiej, E
    Spiewak, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/840762.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    infection
    serological diagnostics
    ELISA test
    Ascaris lumbricoides
    child
    protein antigen
    serological investigation
    Opis:
    The study included 260 hospitalised children with suspected infection with human ascaris. In serological diagnostics a protein antigen obtained from Ascaris lumbricoides was used. ELISA method was applied. IgG antibodies were detected. Positive results were found in 15% of the examined children. No relation to the gender or demographic conditions was found. The most frequently observed symptoms in the patients with Ascaris lumbricoides were: abdominal pain - 87%. diarrhoea 15%. In 31% of the cases eosinophilia was found. Scatoscopy was carried out for all the patients, using the PARASEPT system and Kato and Miura methods as well as decantation and tlotation. The examination. which was repeated three times, did not show cysts or eggs. Serological investigation exhibits higher sensitivity than the traditional methods. Their use in recognising ascariasis in humans significantly facilitates diagnostic procedures, especially in the lung phase of the disease, the larval stage or in cases of infection with an individual parasite, when the faeces samples do not contain the eggs. Serological investigation is also useful in all cases of suspected VLM.
    Źródło:
    Annals of Parasitology; 2001, 47, 4
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serological diagnostics and molecular identification of tick-borne infections in individuals with human immunodeficiency virus type 1 (HIV-1) infection
    Autorzy:
    Welc-Faleciak, R.
    Bednarska, M.
    Radkowski, M.
    Kowalska, J.
    Pawelczyk, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/6706.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    serological diagnostics
    molecular identification
    tick-borne infection
    Borrelia burgdorferi
    Babesia
    Anaplasma phagocytophilum
    Ehrlichia
    Rickettsia
    HIV-1
    human infection
    HIV-1 infection
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Brucellosis in humans - etiology, diagnostics, clinical forms
    Autorzy:
    Galinska, E.M.
    Zagorski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50412.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    brucellosis
    human disease
    etiology
    diagnostics
    clinical form
    Brucella
    occupational exposure
    serological diagnostics
    etiological factor
    Brucella melitensis
    Brucella abortus
    Brucella suis
    Brucella canis
    Brucella neotomae
    Brucella ovis
    Brucella marina
    Brucella microti
    Brucella inopinata
    Opis:
    Brucellosis in humans is a zoonosis of greatly varied clinical image. It occurs on all inhabited continents. The course of the disease may be acute, sub-acute or chronic. The etiologic factors of brucellosis are small, aerobic Gram-negative rods of the genus Brucella, which currently contains ten species: B. abortus, B. suis, B. ovis, B. melitensis, B. canis, B. neotomae, B. pinnipedialis, B. ceti, B. microti and B. inopinata. In humans, the disease is caused mainly by: B. melitensis as the most pathogenic species, followed by B. suis, whereas B. abortus is considered as the mildest type of brucellosis. The natural reservoir of the germ and the source of infection in humans are infected domestic animals, primarily cattle, sheep, goats, as well as wild animals. Infection in humans occurs by penetration through damaged skin, conjunctiva, and more rarely via the alimentary route by the consumption of infected products. Especially exposed are: veterinarians, veterinary technicians, insemination service employees, zoo technicians, farmers working on multi-herd farms (production cooperatives), e.g. cattlemen, also private farmers, employees of slaughter houses and meat processing enterprises. A basis for diagnosing brucellosis are serologic tests which allow the detection of antibodies occurring in response to infection, performed with the use of the following methods: agglutination test, complement fixation test, Coombs test, 2-mercaptoethanol agglutination test, and Burnet’s intradermal allergy test which detects the state of hypersensitivity of the infected organism to Brucella abortus rods.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of ultrasonography in the liver echinococcosis diagnostics
    Autorzy:
    Owczarczyk-Skoczynska, M.
    Grzeszczuk, A.
    Olenski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/838847.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    hydatid cyst
    serological test
    Polska
    echinococcosis
    cystic hydatid disease
    liver
    ultrasonography
    diagnostics
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Q fever - selected issues
    Autorzy:
    Bielawska-Drozd, A.
    Cieslik, P.
    Mirski, T.
    Bartoszcze, M.
    Knap, J.P.
    Gawel, J.
    Zakowska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/49820.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Q fever
    animal species
    man
    Coxiella burnetii
    intracellular parasite
    parasite
    htpAB gene
    IS1111 gene
    genome
    plasmid
    pathomechanism
    infection
    bioterrorism
    epidemiology
    epizootiology
    treatment
    prophylaxis
    laboratory diagnostics
    serological method
    molecular diagnostics
    Opis:
    Q fever is an infectious disease of humans and animals caused by Gram-negative coccobacillus Coxiella burnetii, belonging to the Legionellales order, Coxiellaceae family. The presented study compares selected features of the bacteria genome, including chromosome and plasmids QpH1, QpRS, QpDG and QpDV. The pathomechanism of infection – starting from internalization of the bacteria to its release from infected cell are thoroughly described. The drugs of choice for the treatment of acute Q fever are tetracyclines, macrolides and quinolones. Some other antimicrobials are also active against C. burnetii, namely, telitromycines and tigecyclines (glicylcycline). Q-VAX vaccine induces strong and long-term immunity in humans. Coxevac vaccine for goat and sheep can reduce the number of infections and abortions, as well as decrease the environmental transmission of the pathogen. Using the microarrays technique, about 50 proteins has been identified which could be used in the future for the production of vaccine against Q fever. The routine method of C. burnetii culture is proliferation within cell lines; however, an artificial culture medium has recently been developed. The growth of bacteria in a reduced oxygen (2.5%) atmosphere was obtained after just 6 days. In serology, using the IF method as positive titers, the IgM antibody level >1:64 and IgG antibody level >1:256 (against II phase antigens) has been considered. In molecular diagnostics of C. burnetii infection, the most frequently used method is PCR and its modifications; namely, nested PCR and real time PCR which detect target sequences, such as htpAB and IS1111, chromosome genes (com1), genes specific for different types of plasmids and transposase genes. Although Q fever was diagnosed in Poland in 1956, the data about the occurrence of the disease are incomplete. Comprehensive studies on the current status of Q fever in Poland, with special focus on pathogen reservoirs and vectors, the sources of infection and molecular characteristics of bacteria should be conducted.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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