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Tytuł:
SSR analysis of genetic changes during artificial ageing of rice seeds stored under gene bank management
Autorzy:
Adeboye, K. A.
Adabale, O. W.
Adetumbi, J. A.
Ayo-Vaughan, M. A.
Daniel, I. O.
Powiązania:
https://bibliotekanauki.pl/articles/2199635.pdf
Data publikacji:
2015-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
seed aging
seed storage
genetic integrity
Opis:
Two experiments were conducted to evaluate ageing-induced genetic changes and to establish physiological thresholds for loss of genetic integrity during ageing or storage of rice seeds. In the first experiment, seeds of 10 varieties of rice were subjected to artificial ageing in chambers conditioned to 55°C and 72±2% RH for 72 hours. In the second experiment, seeds of 4 varieties of rice stored in the NACGRAB gene bank, Nigeria in 2011 at 5± 4°C were compared with seeds of the same accessions freshly harvested in 2013. Data were collected on seed germination and seedling length to estimate the seed vigour index. Genetic changes during the ageing were evaluated by SSR markers using a Direct™ PCR kit. Genetic distance indices were computed using PAST™ software and percentage genetic integrity was estimated from the genetic distance matrices. At 72 hours of artificial ageing, seed germination percentage declined to 54.2% and vigour index 0.8 coinciding with the lowest estimate of genetic integrity of 99.5%. The decline in percentage genetic integrity during the artificial ageing indicated a systematic ageing-induced genetic alteration. SSR primer RM178 revealed variations that suggest losses of alleles in the course of ageing for 2 accessions at between 24 hours in WITA4 and 48 to 51 hours in CG14. In the gene bank storage trial, germination of seeds after one and two years of storage was above 80% and there were no significant differences among the accessions. SSR profiles for all the accessions were also similar. The result partly corroborates the artificial ageing data. This implies that seed viability benchmark of 54% is recommended for regeneration of stored rice seeds in order to maintain optimum genetic integrity during storage.
Źródło:
Plant Breeding and Seed Science; 2015, 71; 37-45
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ krótkoterminowego przechowywania nasion osiemnastu genotypów pomidora (Solanum lycopersicum L.) na ich kiełkowanie
Effect of short-term storage of seeds of eighteen tomato (Solanum lycopersicum L.) genotypes on their germination
Autorzy:
Chojnowski, M.
Wawrzyniak, A.
Powiązania:
https://bibliotekanauki.pl/articles/2118938.pdf
Data publikacji:
2021
Wydawca:
Instytut Ogrodnictwa
Tematy:
tomato
genotypes
seed storage
germination
primary dormancy
Opis:
The aim of the research was to determine the occurrence of primary dormancy in seeds of 18 tomato genotypes (Solanum lycopersicum L.) – 16 cultivars and two local genotypes – regenerated for the active collection of the National Institute for Horticultural Research. The plants were grown in the field in accordance with the adopted methodology of seed reproduction in the collection of genetic resources of vegetable plants. Tomato fruits were harvested twice a week from July 18 to September 26, 2019. Immediately after harvest, the seeds were extracted from the fruit, cleaned and dried according to FAO standards for gene banks. The seeds prepared in this way were stored for a period of sixty weeks at a temperature of 4±2°C in airtight containers with the addition of silica gel. During this period, after twenty, forty and sixty weeks of storage, their germination was tested. The studied tomato genotypes differed significantly in terms of depth of primary dormancy and rate of releasing from dormancy. Two of the tested cultivars – ‘Parteno’ and ‘Reper’, were characterized by seed dormancy at the level of 44.5% and 35.5%, two genotypes – ‘Amber’ and the local genotype POLPOB17-27 were characterized by dormancy levels of 15% and 18%. Seeds of two of the genotypes studied that did not show primary dormancy twenty weeks after harvest – ‘Zealand’ and the local genotype POLPOB17-28 – after storage for sixty weeks showed a reduced germination rate and a longer mean germination time. It is concluded that the optimum time for testing the germination of tomato seeds stored after harvest at 4 °C is forty weeks after harvest. The level of primary dormancy of the seeds is then the lowest, but the induction of secondary dormancy does not occur yet. When testing the seed germination in an earlier period, dormancy breaking procedures should be considered to obtain correct results.
Źródło:
Zeszyty Naukowe Instytutu Ogrodnictwa; 2021, 29; 15-24
2300-5882
2391-8969
Pojawia się w:
Zeszyty Naukowe Instytutu Ogrodnictwa
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of spermidine on germination of Salix spp. after storage under controlled conditions
Autorzy:
Wawrzyniak, M.K.
Ley-Lopez, J.M.
Kijowska-Oberc, J.
Chmielarz, P.
Ratajczak, E.
Powiązania:
https://bibliotekanauki.pl/articles/2130180.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
hydrogen peroxide
cryopreservation
spermidine
Salix
antioxidant
seed storage
Opis:
Willows produce fast germinating and short-lived seeds, difficult to store in the long-term under controlled conditions. The aim of this study was to examine the feasibility of storage of three Salix spp. at controlled temperatures (3°, −10°, −196 °C). We also analyzed the effect of spermidine (Spd) as an antioxidant factor in desiccated seeds. Collected seeds were either desiccated or hydrated to obtain 10 levels of moisture content (between app. 4% and 2%) and subjected to storage at temperatures 3°, −10°, or −196 °C (liquid nitrogen; LN). After two months, seeds were germinated on the light at 20 °C. Seeds desiccated below a safe range of moisture content were further tested and germinated on filter paper with additions of 0.25 mM Spd solution. After 7 days seedlings were examined for hydrogen peroxide content (H2O2) and total antioxidant capacity (TAC). Fresh seeds of three Salix species: Persian willow (S. aegyptiaca L.), heartleaf willow (S. cordata Michx.) and crack willow (S. ×fragilis L.) were successfully stored at temperature −10° and −196 °C for two months. After cryopreservation seed of S. aegyptiaca, S. cordata, and S. ×fragilis germinated without viability loss in moisture content ranging from 4.4–15.9%, 6.4–18.5%, and 7.1–11.5% respectively. The addition of Spd during germination of desiccated seed did not affect germination capacity. However, seedlings of S. aegyptiaca had lower hydrogen peroxide content in comparison with control (germination on water). Seedlings of S. cordata showed an increase in hydrogen peroxide content in control after storing in LN. In seedlings of Crack willow Spd increased hydrogen peroxide content. Seeds of tested species differ in response to storage conditions. Salix seeds can be stored successfully for two months at −10° or −196 °C without losing viability in the safe range of moisture content. Storing at 3 °C can be used for storage in the narrower range of seeds’ moisture content, however, seedlings stored at this temperature produce a higher level of reactive oxygen species. Germinating seeds in Spd did not increase their germination, however in S. aegyptiaca and S. cordata decreased hydrogen peroxide content.
Źródło:
Dendrobiology; 2022, 87; 137-148
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selected aspects of tiny vetch [Vicia hirsuta (L.) Gray S.F.] seed ecology: generative reproduction and effects of seed maturity and seed storage on seed germination
Wybrane aspekty ekologii nasion wyki drobnokwiatowej [Vicia hirsuta (L.) Gray F.S.]: reprodukcja generatywna oraz wpływ stopnia dojrzałości i warunków przechowywania na kiełkowanie nasion
Autorzy:
Kucewicz, M.
Mackiewicz, K.
Zrobek-Sokolnik, A.
Powiązania:
https://bibliotekanauki.pl/articles/26682.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
tiny vetch
Vicia hirsuta
seed ecology
generative reproduction
seed maturity
seed storage
seed germination
Opis:
Vicia hirsuta (L.) Gray S.F. (tiny vetch) is a common and persistent segetal weed. Tiny vetch seeds and pods reach different stages of maturity during the crop harvest season. Some seeds that mature before cereal harvest are shed in the field and deposited in the soil seed bank, while others become incorporated into seed material. The objective of this study was to describe selected aspects of tiny vetch seed ecology: to determine the rate of individual reproduction of vetch plants growing in winter and spring grain crops and to evaluate the germination of seeds at different stages of maturity, subject to storage conditions. The seeds and pods of V. hirsuta were sorted according to their development stages at harvest and divided into two groups. The first group was stored under laboratory conditions for two months. In the autumn of the same year, the seeds were subjected to germination tests. The remaining seeds were stored in a storeroom, and were planted in soil in the spring. The germination rate was evaluated after 8 months of storage. Potential productivity (developed pods and flowers, fruit buds) was higher in plants fruiting in winter wheat than in spring barley. Vetch plants produced around 17-26% more pods (including cracked, mature, greenish-brown and green pods) and around 25% less buds in winter wheat than in spring barley. Immature seeds were characterized by the highest germination capacity. Following storage under laboratory conditions and stratification in soil, mature seeds germinated at a rate of several percent. After storage in a storeroom, seeds at all three development stages broke dormancy at a rate of 72- 75%. The high germination power of tiny vetch seeds stored in a storeroom indicates that this plant can be classified as an obligatory speirochoric weed species.
Źródło:
Acta Agrobotanica; 2010, 63, 1
0065-0951
2300-357X
Pojawia się w:
Acta Agrobotanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Betula pendula seed storage and sowing pre-treatment: effect on germination and seedling emergence in container cultivation
Autorzy:
Tylkowski, T.
Powiązania:
https://bibliotekanauki.pl/articles/41787.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Betula pendula
seed storage
sowing
pretreatment
germination
seedling emergence
container cultivation
Opis:
Irregular seed crop in Betula pendula (Silver Birch) is a reason to create seed reserves, ensuring nursery production in years of crop failure. This study investigated the effects of seed moisture content and storage temperature on germinability and seedling emergence in container cultivation. Mature catkins were collected separately from 3 trees. The mixture of winged nuts and scales was dried to 3 levels of moisture content and stored at 3°C, –3°C and –10°C. After storage for 3, 5 and 6 years, the seeds were separated from scales and next dewinged and sorted in acetone into empty and filled seeds. Cleaned nuts were germinated in the Jacobsen germinator. Besides, to assess seedling emergence, seeds were sown on the surface of peat mixed with perlite, in multi-cell trays under a plastic tunnel. Significant differences in germinability were detected depending on the mother tree, seed moisture content and storage time. The viability of seeds stored at –10°C remained unchanged for 6 years regardless of moisture content (ca. 8–12%). Seeds stored at higher temperatures lost their germinability faster. An unexplained increase in seedling emergence was observed after extended seed storage at –10°C, in contrast to a gradual decrease in seedling emergence after extended storage at –3°C.
Źródło:
Dendrobiology; 2012, 67
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hydrotime model analysis of cornflower achene germination
Autorzy:
Bochenek, A.
Golaszewski, J.
Kondrat, B.
Szymczak, M.
Jagielska, T.
Powiązania:
https://bibliotekanauki.pl/articles/80450.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
seed dormancy
hydrotime model
mature seed
Centaurea cyanus
seed storage
germination
water stress
weather condition
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity of common bean genotypes as revealed by seed storage proteins and some agronomic traits
Autorzy:
Marzooghian, Akbar
Moghaddam, Mohammad
Valizadeh, Mostafa
Kooshki, Mohammad Hasan
Powiązania:
https://bibliotekanauki.pl/articles/2199727.pdf
Data publikacji:
2012-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
agronomic traits
genetic diversity
Phaseolus vulgaris L.
SDS-PAGE
seed storage proteins
Opis:
Evaluation of the genetic diversity present within species is essential for conservation, management and utilization of the genetic resources. The objective of this study was to evaluate genetic variability of 70 com- mon bean genotypes for seed storage proteins, grain morphological characteristics and agronomic traits. Two methods of extracting soluble seed proteins in salt were used. Positive correlations were observed among both seed morphological characters and developmental charac- ters while yield components showed negative correlations with each other. Factor analysis for agronomic and grain  morphological  traits  resulted  in three factors  were named yield  components,   seed morphology  and phenology, respectively. Most genotypes had lower or medium scores for yield components and phenology factors. Considerable diversity was observed for seed morphology factor among the common bean genotypes. Nei’s diversity coefficient (He = 0.4), effective number of alleles (Ae  = 1.69) and number of polymorphic loci (N = 17) indicated larger variation in the extraction method of soluble proteins in low salt (0.2 M NaCl) than high salt (1 M NaCl) condition. Considering that the centers of diversity for common  bean are different in seed size, the result of GST statistics showed that bands with relative mobility of 30, 32, 38 and 40 differen- tiated two weight groups more than other bands. Furthermore, significant differences were observed between these bands for number of pods per plant and number of seeds per plant.
Źródło:
Plant Breeding and Seed Science; 2012, 67; 125-137
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
On the geographic variation of Ciboria batschiana (Zopf) Buchwald, the main pathogenic fungus on acorns of Quercus robur and Q.petraea in Europe
Autorzy:
Schroder, T
Powiązania:
https://bibliotekanauki.pl/articles/41225.pdf
Data publikacji:
2002
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
seed-borne fungi
seed storage
geographic variation
Ciboria batschiana
acorn
pathogenic fungi
fungi
Quercus robur
Quercus petraea
Opis:
Mycelial growth of the pathogenic fungus Ciboria batschiana (Zopf) Buchwald was examined at nine temperature levels between -3°C and +35°C using pure cultures of the fungus from eleven different provenances located in Germany, Poland, the Czech Republic and Slovenia. The fungus was isolated mainly from cotyledons of acorns of the oak species Quercus robur L. and Quercus petraea (Matt.) Liebl. Even at -3°C the mycelium of the fungus grew well. There were significant differences between the provenances in respect to growth rate and temperature tolerance, especially at low temperatures.
Źródło:
Dendrobiology; 2002, 47 Supplement
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
GGT-assisted glutathione degradation is functional to apoplastic redox control and seed storage proteins accumulation
Autorzy:
Trentin, A.
Pivato, M.
Giaretta, S.
Fabrega-Prats, M.
Arrigoni, G.
Prasad, D.
Masi, A.
Powiązania:
https://bibliotekanauki.pl/articles/80200.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
gamma-glutamyl cycle
redox control
seed storage
abiotic stress
biotic stress
carbohydrate metabolism
photosynthesis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seed storage, germination and seedling emergence in Rhamnus catharticus
Autorzy:
Tylkowski, T
Powiązania:
https://bibliotekanauki.pl/articles/41131.pdf
Data publikacji:
2007
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
common buckthorn
Rhamnus catharticus
North America
seed storage
germination
seedling emergence
invasive species
landscape shrub
shrub
tree
medicinal plant
Opis:
This study was aimed to improve the understanding of germination ecology and to explain the invasive character of the common buckthorn (Rhamnus catharticus) in North America. Its fully mature seeds are characterized by a lack of dormancy. In laboratory conditions, favourable thermal conditions were identified for seed storage, germination and seedling emergence. At the cyclically alternating temperature of 20~30°C (16+8 h daily), seeds of this species showed a high germination rate within few weeks. Two other thermal variants, 3~15°C and 3~20°C (16+8 h daily), were much less effective for seed germination. However, cold stratification (at 3°C) in a moist mixture of peat and sand, lasting 4–8 weeks, caused a remarkable increase in germination rate also at 3~15°C. Seeds extracted from ripe fruits and dried (to a moisture content of about 10%) showed high germination and emergence rates after storage for 3.5 years at –3°C.
Źródło:
Dendrobiology; 2007, 58; 67-72
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
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