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    Wyświetlanie 1-3 z 3
    Tytuł:
    Can changes in starch content and peroxidase activity be used as rooting phase markers for rhododendron leaf bud cuttings?
    Autorzy:
    Megre, D.
    Dokane, K.
    Kondratovics, U.
    Powiązania:
    https://bibliotekanauki.pl/articles/19397.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    content change
    starch content
    peroxidase activity
    rooting phase marker
    rhododendron
    leaf bud cutting
    propagation
    rooting
    Opis:
    We examined whether peroxidase activity in cutting bases and leaves and starch content in cutting bases can be used as rooting phase markers in the elepidote rhododendron cv. 'Babites Baltais' (Rhododendron L.). Changes in peroxidase activity in cutting leaves and bases, as well as starch content in cutting bases, were determined in relation to anatomical stages of rhizogenesis in leaf bud cuttings treated with 1% indole-3-butyric acid (IBA+) or without IBA (IBA–). The pattern of change of peroxidase activity was similar in cutting bases and leaves of IBAleaf bud cuttings. Three phases of adventitious root formation were identified: induction, initiation and expression. During the induction phase peroxidase activity decreased, but no anatomical changes were observed in the cuttings. Peroxidase activity increased in the initiation phase when adventitious root initials were formed. Peroxidase activity decreased during the expression phase when adventitious root primordia developed. The starch content of IBA– leaf bud cuttings decreased during the first few days and then gradually rose to maximum, followed by a sharp reduction and another increase at the end of the experiment. The changes of starch content did not coincide with rooting phases as peroxidase activity did, and cannot be used as a rooting phase marker in rhododendrons. Adventitious root formation did not occur in IBA+ leaf bud cuttings, so distinct rooting phases could not be observed. There was a significant correlation between peroxidase activity in cutting bases and leaves of IBA- leaf bud cuttings. Peroxidase activity in leaves of rhododendron leaf bud cuttings are potentially useful as a marker for rooting phases, but that requires further anatomical and physiological study of rooting in leaf bud cuttings.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effectiveness of Daphne L. (Thymelaeaceae) in vitro propagation, rooting of microshoots and acclimatization of plants
    Efektywność rozmnażania in vitro Daphne L. (Thymelaeaceae), ukorzeniania mikrosadzonek i aklimatyzacji roślin
    Autorzy:
    Hanus-Fajerska, E.
    Wiszniewska, A.
    Czaicki, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/28397.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    effectiveness
    Daphne
    Thymelaeaceae
    in vitro propagation
    rooting
    microshoot
    plant acclimatization
    regeneration capacity
    micropropagation
    rooting efficacy
    shrub
    plant species
    Daphne caucasica
    Daphne jasminea
    Daphne tangutica
    rooting phase
    greenhouse condition
    Opis:
    In this study, an attempt was made to investigate in vitro morphogenetic competence of three shrub species from the Thymelaeaceae family. The studied plant material originated from Russia, Greece and China, and the effectiveness of in vitro shoot formation and rhizogenesis of Daphne caucasica, D. jasminea, and D. tangutica was verified. The multiplication coefficient was compared for different propagation media. Medium composed of WPM mineral salts, MS microelements and a set of vitamins, supplemented with 1.0 mg dm-3 2iP, 0.1 mg dm-3 NAA, and 0.65 g dm-3 calcium gluconate, was appropriate for micropropagation of the tested genotypes. Shoot propagation in medium containing B5 vitamins and microelements was not as effective as on WPM/MS medium. The rooting phase, especially in D. tangutica, needs further optimization in order to reduce the costs associated with acclimatization of microplantlets obtained to in vivo conditions. After stabilization, the plants were successfully cultivated under greenhouse conditions.
    Badano kompetencję morfogenetyczną trzech gatunków roślin krzewiastych z rodziny wawrzynkowatych. Materiał roślinny pochodził z Rosji, Grecji i Chin. Regenerowano in vitro pędy i korzenie przybyszowe u wawrzynka kaukaskiego (Daphne caucasica), wawrzynka jaśminowego (D. jasminea) oraz wawrzynka tanguckiego (D. tangutica). Pożywka składająca się z zestawu soli mineralnych dla roślin drzewiastych (WPM), mikroelementów i witamin według Murashige i Skooga (MS), wzbogacona o 1.0 mg×dm-3 2iP, 0.1 mg×dm-3 NAA i 0.65 g×dm-3 glukonianu wapnia okazała się odpowiednia do mikrorozmnażania badanych genotypów. Namnażanie pędów na pożywce zawierającej zestaw witamin według Gamborga i współpracowników (B5) było mniej efektywne niż na pożywce WPM/MS. Etap ukorzeniania, szczególnie dla wawrzynka tanguckiego, wymaga dalszej optymalizacji w celu zmniejszenia kosztów ponoszonych w trakcie uzyskiwania materiału przydatnego do uprawy. Otrzymane drogą rozmnażania in vitro rośliny przystosowano do uprawy w warunkach szklarniowych.
    Źródło:
    Acta Agrobotanica; 2012, 65, 1
    0065-0951
    2300-357X
    Pojawia się w:
    Acta Agrobotanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on solid, liquid and double-phase culture systems
    Autorzy:
    Kaviani, B.
    Bahari, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/12678778.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Gentianaceae
    Eustoma
    Lisianthus
    Eustoma grandiflorum
    ornamental plant
    flowering plant
    rooting
    microshoot
    micropropagation
    in vitro proliferation
    ex vitro method
    soild phase
    liquid phase
    double-phase culture
    plant growth regulator
    auxin
    cytokinin
    Opis:
    A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and 5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with 0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 47-56
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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