Temat: ribonuclease Dicer - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Human ribonuclease Dicer inhibition using RNA aptamers
Autorzy:: Tyczewska, A.
Powiązania:: https://bibliotekanauki.pl/articles/80081.pdf
Data publikacji:: 2014
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: ribonuclease
ribonuclease Dicer
aptamer
SELEX method
microRNA
biogenesis
enzyme activity
patent
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2014, 95, 4
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: How short RNAs impact the human ribonuclease Dicer activity: putative regulatory feedback-loops and other RNA-mediated mechanisms controlling microRNA processing
Autorzy:: Koralewska, Natalia
Hoffmann, Weronika
Pokornowska, Maria
Milewski, Marek
Lipinska, Andrea
Bienkowska-Szewczyk, Krystyna
Figlerowicz, Marek
Kurzynska-Kokorniak, Anna
Powiązania:: https://bibliotekanauki.pl/articles/1038739.pdf
Data publikacji:: 2016
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: ribonuclease Dicer
miRNA processing
regulatory RNAs
regulation of Dicer activity
regulatory feedback-loops
Opis:: Ribonuclease Dicer plays a pivotal role in RNA interference pathways by processing long double-stranded RNAs and single-stranded hairpin RNA precursors into small interfering RNAs (siRNAs) and microRNAs (miRNAs), respectively. While details of Dicer regulation by a variety of proteins are being elucidated, less is known about non-protein factors, e.g. RNA molecules, that may influence this enzyme's activity. Therefore, we decided to investigate the question of whether the RNA molecules can function not only as Dicer substrates but also as its regulators. Our previous in vitro studies indicated that the activity of human Dicer can be influenced by short RNA molecules that either bind to Dicer or interact with its substrates, or both. Those studies were carried out with commercial Dicer preparations. Nevertheless, such preparations are usually not homogeneous enough to carry out more detailed RNA-binding studies. Therefore, we have established our own system for the production of human Dicer in insect cells. In this manuscript, we characterize the RNA-binding and RNA-cleavage properties of the obtained preparation. We demonstrate that Dicer can efficiently bind single-stranded RNAs that are longer than ~20-nucleotides. Consequently, we revisit possible scenarios of Dicer regulation by single-stranded RNA species ranging from ~10- to ~60-nucleotides, in the context of their binding to this enzyme. Finally, we show that siRNA/miRNA-sized RNAs may affect miRNA production either by binding to Dicer or by participating in regulatory feedback-loops. Altogether, our studies suggest a broad regulatory role of short RNAs in Dicer functioning.
Źródło:: Acta Biochimica Polonica; 2016, 63, 4; 773-783
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: RNA oligomers as microRNA production process regulators
Autorzy:: Kurzynska-Kokorniak, A.
Koralewska, N.
Tyczewska, A.
Twardowski, T.
Figlerowicz, M.
Powiązania:: https://bibliotekanauki.pl/articles/80185.pdf
Data publikacji:: 2014
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: oligomer
RNA oligomer
microRNA
ribonuclease Dicer
biogenesis
endoribonuclease
double stranded RNA
patent
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2014, 95, 4
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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