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Wyszukujesz frazę "restriction analysis" wg kryterium: Temat


Wyświetlanie 1-2 z 2
Tytuł:
Restriction analysis of genetic variability of Polish isolates of Tomato black ring virus.
Autorzy:
Jończyk, Magdalena
Borodynko, Natasza
Pospieszny, Henryk
Powiązania:
https://bibliotekanauki.pl/articles/1041544.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
restriction analysis
genetic variability
phylogeny
Tomato black ring virus
Opis:
Several different isolates of Tomato black ring virus (TBRV) have been collected in Poland from cucumber, tomato, potato and black locust plants. Biological tests showed some differences in the range of infected plants and the type of symptoms, which was the basis for selection of seven the most biologically different TBRV isolates. According to the sequence of TBRV-MJ, several primer pairs were designed and almost the entire sequence of both genomic RNAs was amplified. The RT-PCR products derived from all tested TBRV isolates were digested by restriction enzymes. On the basis of the restriction patterns, the variable and the conserved regions of the TBRV genome were defined and the relationships between the Polish TBRV isolates established.
Źródło:
Acta Biochimica Polonica; 2004, 51, 3; 673-681
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction
Autorzy:
Chen, Huiqing
Li, Yijia
Chen, Keping
Yao, Qin
Li, Guohui
Wang, Lin
Powiązania:
https://bibliotekanauki.pl/articles/1040312.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
calorie restriction
Bombyx mori
two-dimensional gel electrophoresis
proteomic analysis
MALDI-TOF/TOF MS
Opis:
Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.
Źródło:
Acta Biochimica Polonica; 2010, 57, 4; 505-511
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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