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Wyszukujesz frazę "real-time quantitative PCR" wg kryterium: Temat

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    Wyświetlanie 1-5 z 5
    Tytuł:
    Cloning and partial characterization of a gene in Bombyx mori homologous to a human adiponectin receptor
    Autorzy:
    Zhu, Minfeng
    Chen, Keping
    Wang, Yong
    Guo, Zhongjian
    Yin, Huijuan
    Yao, Qin
    Chen, Huiqin
    Powiązania:
    https://bibliotekanauki.pl/articles/1040735.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    BmAdipoR
    bioinformatics study
    subcellular localization
    real-time quantitative PCR
    BmNPV
    Opis:
    In this study, we report the cloning and characteristics of an adiponectin-like receptor gene from Bombyx mori (BmAdipoR) with highly conserved deduced amino-acid sequences and similar structure to the human adiponectin receptor (AdipoR). Structural analysis of the translated cDNA suggested it encoded a membrane protein with seven transmembrane domains. BmAdipoR was found to be expressed in multiple tissues and highly expressed in Malpighian tubules, fat body and testis. BmNPV (Bombyx mori nucleopolyhedrovirus) bacmid system combined with confocal microscopy revealed that BmAdipoR was targeted to the cell membrane. We also found that infection with BmNPV did not have an effect on BmAdipoR mRNA quantity in the midgut of susceptible Bombyx mori strain (306) at 48 h, but BmAdipoR mRNA quantity increased significantly at 72 h. We concluded that BmAdipoR gene was a membrane protein ubiquitously expressed in Bombyx mori tissues and that its expression was altered by treating with BmNPV.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 2; 241-249
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    TaqMan real-time PCR for detecting bovine viral diarrhea virus
    Autorzy:
    Liang, H.
    Geng, J.
    Bai, S.
    Aimuguri, A.
    Gong, Z.
    Feng, R.
    Shen, X.
    Wei, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087557.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    bovine viral diarrhea virus
    quantitative real time PCR
    TaqMan probe
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 2; 405-413
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The profile of ErbB/Her family genes copy number assessed by real-time PCR in parathyroid adenoma and hyperplasia associated with sporadic primary hyperparathyroidism
    Autorzy:
    Bednarz, Natalia
    Błaut, Krzysztof
    Sworczak, Krzysztof
    Osęka, Tomasz
    Bielawski, Krzysztof
    Powiązania:
    https://bibliotekanauki.pl/articles/1040637.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    parathyroid hyperplasia
    hyperparathyroidism
    parathyroid adenoma
    ErbB/Her
    quantitative real-time PCR
    Opis:
    Hyperparathyroidism (pHPT) is a relatively frequent endocrinopathy, however, the molecular mechanisms of its etiology remain poorly understood. This disorder is mainly associated with benign tumours (adenoma) and hyperplasia of the parathyroid, hence, the focus is directed also to genes that are likely to be involved in carcinogenesis. Among such genes are ErbB/Her family genes already used in diagnosis of other tumours (e.g., breast carcinoma) and reported also to play a role in development of endocrine lesions. So far, ErbB-1/Her-1/EGFR expression has been detected in pHPT-associated adenomas and hyperplasia as opposed to no expression in normal parathyroid tissue. Moreover, losses or gains of the fragments of chromosomes where ErbB/Her genes are located have been reported. In this study, the gene dosage of ErbB/Her family genes were determined for the first time in parathyroid adenomas, hyperplasia and morphologically unchanged tissue in order to establish their putative role in the development of the disease. Genomic DNA was isolated from 33 patients with sporadic hyperparathyroidism and the gene copy numbers were assessed using real-time PCR. The ErbB/Her genes' profile was unaltered in most of the examined samples. Two low-level amplifications of ErbB-1/Her-1/EGFR gene, two deletions of ErbB-2/Her-2, and six deletions of ErbB-4/Her-4 were found. The ErbB-3/Her-3 gene remained unaffected. No correlation with clinical parameters was found for any gene. Both the low number of alterations and a lack of their associations with clinical parameters exclude the prognostic value of the ErbB/Her genes family in parathyroid tumourigenesis. Nevertheless, the ErbB-4/Her-4 deletions seem to be interesting for further investigations, especially in the context of PTH secretion.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 1; 83-88
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Changes in accumulation of heteroplasmic mitochondrial DNA and frequency of recombination via short repeats during plant lifetime in Phaseolus vulgaris
    Autorzy:
    Woloszynska, Magdalena
    Gola, Edyta
    Piechota, Janusz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039695.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Phaseolus vulgaris
    sublimons
    heteroplasmy
    quantitative real-time PCR
    plant mitochondrial genome
    mtDNA recombination
    Opis:
    Recombination via short repeats in plant mitochondrial genomes results in sublimons - DNA molecules with a copy number much lower compared to the main mitochondrial genome. Coexistence of stoichiometrically different mitotypes, called heteroplasmy, plays an important evolutionary role, since sublimons occasionally replace the main genome resulting in a new plant phenotype. It is not clear, how frequency of recombination and sublimon production is regulated and how it is related to changes in the quantity of the main genome and sublimons. We analyzed the accumulation of two recombining main genome sequences and two resulting sublimons in apical meristems, undifferentiated tissues and leaves of different age of Phaseolus vulgaris. Copy numbers of the main genome sequences varied greatly depending on tissue type and organ age while accumulation of sublimons remained much more stable. Although the overall accumulation of plant mtDNA decreased with the leaf age, the quantity of sublimons increased relative to the main genome indicating a higher frequency of recombination via the short 314 bp repeat. Recombination was symmetrical in young developing leaves while in senescent tissues it shifted towards asymmetric events resulting in overrepresentation of one product. We propose that during plant lifetime replication and recombination frequencies change oppositely sustaining heteroplasmic compositions of the genome, which are favorable for inheritance and maintenance of complex plant mtDNA.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 4; 703-709
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The expression of androgen receptor in neurons of the anterior pelvic ganglion and celiac-superior mesenteric ganglion in the male pig
    Autorzy:
    Kaleczyc, J.
    Kasica-Jarosz, N.
    Pidsudko, Z.
    Przyborowska, A.
    Sienkiewicz, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087598.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    androgen receptor
    neurons
    anterior pelvic ganglion
    celiac-superior mesenteric ganglion
    quantitative real-time PCR
    immunohistochemistry
    male pig
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 1; 151-155
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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